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Machine translation
1. (WO2003054162) METHOD AND SYSTEM FOR DEPLETING rRNA POPULATIONS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2003/054162    International Application No.:    PCT/US2002/041014
Publication Date: 03.07.2003 International Filing Date: 19.12.2002
Chapter 2 Demand Filed:    20.06.2003    
IPC:
C12N 15/10 (2006.01), C12N 15/11 (2006.01)
Applicants: AMBION, INC. [US/US]; 2130 Woodward St., Suite 200, Austin, TX 78744-1832 (US) (For All Designated States Except US).
MURPHY, George, L. [US/US]; (US) (For US Only).
WHITLEY, J., Penn [US/US]; (US) (For US Only)
Inventors: MURPHY, George, L.; (US).
WHITLEY, J., Penn; (US)
Agent: SHISHIMA, Gina, N.; Fulbright & Jaworski L.L.P., Suite 2400, 600 Congress Avenue, Austin, TX 78701 (US)
Priority Data:
10/029,397 20.12.2001 US
Title (EN) METHOD AND SYSTEM FOR DEPLETING rRNA POPULATIONS
(FR) PROCEDE ET SYSTEME D'APPAUVRISSEMENT DE POPULATIONS D'ARNR
Abstract: front page image
(EN)The present invention concerns a system for isolating, depleting, or separating a targeted nucleic acid, such as rRNA, from a sample comprising targeted and nontargeted nucleic acids. It effects a way of enriching for nontargeted nucleic acids, such as mRNAs. The invention further concerns methods of implementing the system and kits for implementing the system, which involves at least one bridging nucleic acid comprising 1) a targeting region complementary to a region on the targeted nucleic acid and 2) a bridging region complementary to the capture region of a capture nucleic acid that comprises a nonreactant structure. The nonreactant structure can be used to isolate the hybridizing molecules after incubation under conditions that allows hybridization.
(FR)L'invention porte sur un système d'isolement, appauvrissement ou séparation d'un acide nucléique ciblé tel qu'un ARNr d'avec un échantillon comprenant des acides nucléiques ciblés ou non ciblés, et consistant en un moyen d'enrichissement en acides nucléiques non ciblés tels que des ARNm. L'invention porte également sur des procédés et des trousses de mise en oeuvre du système, qui comprennent au moins un acide nucléique passerelle comportant: 1) une région à cibler complémentaire de l'acide nucléique ciblé, et 2) une région passerelle complémentaire de la région d'un acide nucléique de capture à structure non réactive, ladite structure pouvant servir à isoler les molécules hybridantes après incubation dans des conditions permettant l'hybridation.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)