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1. (WO2003052134) OLIGONUCLEOTIDES COMPRISING INTERCALATOR PSEUDONUCLEOTIDE(S) FOR DETECTION OF NUCLEIC ACIDS AND MUTANTS HEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2003/052134 International Application No.: PCT/DK2002/000877
Publication Date: 26.06.2003 International Filing Date: 18.12.2002
Chapter 2 Demand Filed: 07.07.2003
IPC:
A61K 38/00 (2006.01) ,C07H 19/04 (2006.01) ,C07H 21/00 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/11 (2006.01) ,C12Q 1/68 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38
Medicinal preparations containing peptides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19
Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro derivatives thereof
02
sharing nitrogen
04
Heterocyclic radicals containing only nitrogen as ring hetero atom
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
HUMAN GENETIC SIGNATURES PTY LTD [--/AU]; 63 Kananook Avenue Bayview New South Wales 2104, AU (AllExceptUS)
CHRISTENSEN, Ulf, Bech [DK/DK]; DK (UsOnly)
PEDERSEN, Erik, Bjerregaard [DK/DK]; DK (UsOnly)
Inventors:
CHRISTENSEN, Ulf, Bech; DK
PEDERSEN, Erik, Bjerregaard; DK
Priority Data:
60/365,54520.03.2002US
PA 2001 0189718.12.2001DK
PA 2001 0189818.12.2001DK
PA 2001 0189918.12.2001DK
PA 2001 0190018.12.2001DK
PA 2002 0157514.10.2002DK
PA 2002 0157614.10.2002DK
PA 2002 0157714.10.2002DK
PA 2002 0157814.10.2002DK
Title (EN) OLIGONUCLEOTIDES COMPRISING INTERCALATOR PSEUDONUCLEOTIDE(S) FOR DETECTION OF NUCLEIC ACIDS AND MUTANTS HEREOF
(FR) OLIGONUCLEOTIDES COMPRENANT UN OU DES PSEUDONUCLEOTIDES INTERCALANT DESTINES A DETECTER DES ACIDES NUCLEIQUES ET DES MUTANTS DE CEUX-CI
Abstract:
(EN) The present invention relates to oligonucleotides or oligonucleotide analogues comprising at least two intercalator pseudonucleotides. Intercalator pseudonucleotides are capable of being incorporated into the backdone of a nucleic acid analogue and comprise an intercalator comprising at least one essentially flat conjugated system, which is capable of co-stacking with nucleobases of a nucleic acid. Furthermore, the invention relates to methods of detecting nucleic acids or nucleic acid analogues comprising a specific target sequence, methods of differentiating between a nucleic acid or nucleic acid analogue comprising a specific target sequence and a nucleic acid comprising a mutant sequence which differs from the target sequence by at least one nucleobase, and methods for detecting a target sequence and/or a mutant sequence, which offer from the target sequence by at least one nucleobase. In preferred embodiments of the invention said methods involve the use of oligonucleotides or oligonucleotide analogues comprising at least two intercalator pseudonucleotides.
(FR) La présente invention concerne des oligonucléotides ou des analogues d'oligonucléotides comprenant au moins deux pseudonucléotides intercalant. Des pseudonucléotides intercalant peuvent être incorporés dans le squelette d'un analogue d'acide nucléique et comprennent un intercalant, lequel comprend au moins un système conjugué sensiblement plat qui est capable de s'empiler avec des nucléobases d'un acide nucléique. Cette invention concerne aussi des techniques de détection d'acides nucléiques ou d'analogues d'acide nucléique comprenant une séquence cible spécifique, des techniques de différentiation entre un acide nucléique ou un analogue d'acide nucléique comprenant une séquence cible spécifique et un acide nucléique comprenant une séquence mutante qui diffère de la séquence cible par au moins une nucléobase, et des techniques de détection d'une séquence cible et/ou d'une séquence mutante qui diffère de la séquence cible par au moins une nucléobase. Dans un mode de réalisation de l'invention, ces techniques consistent à utiliser des oligonucléotides ou des analogues d'oligonucléotide comprenant au moins deux pseudonucléotides intercalant.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)