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1. (WO2003046212) METHOD FOR IDENTIFYING TARGET EPITOPES OF THE T CELL MEDIATED IMMUNE RESPONSE AND FOR ASSAYING EPITOPE-SPECIFIC T CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2003/046212 International Application No.: PCT/DE2002/004270
Publication Date: 05.06.2003 International Filing Date: 20.11.2002
IPC:
C07K 14/705 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/85 (2006.01) ,G01N 33/50 (2006.01) ,G01N 33/68 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
DEML, Ludwig [DE/DE]; DE
Inventors:
DEML, Ludwig; DE
Agent:
BETTENHAUSEN, Berthold ; Dehmel & Bettenhausen Müllerstr. 1 80469 München, DE
Priority Data:
101 56 863.020.11.2001DE
Title (EN) METHOD FOR IDENTIFYING TARGET EPITOPES OF THE T CELL MEDIATED IMMUNE RESPONSE AND FOR ASSAYING EPITOPE-SPECIFIC T CELLS
(FR) PROCEDE D'IDENTIFICATION D'EPITOPES CIBLES DE LA REPONSE IMMUNITAIRE INDUITE PAR LES LYMPHOCYTES T ET DE DETECTION DES LYMPHOCYTES T SPECIFIQUES A L'EPITOPE
(DE) VERFAHREN ZUR IDENTIFIZIERUNG VON ZIELEPITOPEN DER T−ZELL−VERMITTELTEN IMMUNANTWORT UND ZUM NACHWEIS EPITOP−SPEZIFISCHER T−ZELLEN
Abstract:
(EN) The invention relates to a method for assaying epitope-specific T cells and target epitopes of reactive T cells. The present invention further relates to vectors, comprising a first promoter that can be specifically induced in antigen-presenting cells by the epitope-specific contact with a T cell, a nucleic acid functionally linked with said first promoter and encoding a marker gene, a second promoter that is constitutive in antigen-presenting cells, and a nucleic acid that is functionally linked with said second promoter. The present invention further relates to antigen-presenting cells that are transduced with the vectors according to the invention.
(FR) La présente invention concerne un procédé de détection de lymphocytes T spécifiques à l'épitope et d'épitopes cibles de lymphocytes T réactifs. La présente invention concerne également des vecteurs contenant un premier promoteur pouvant être induit spécifiquement dans les cellules présentant un antigène par contact spécifique à l'épitope avec un lymphocyte T, un acide nucléique fonctionnellement lié à ce premier promoteur et codant un gène marqueur, un deuxième promoteur constitutif dans les cellules présentant un antigène, et un acide nucléique fonctionnellement lié à ce deuxième promoteur. La présente invention concerne également des cellules présentant un antigène et transduites par les vecteurs selon l'invention.
(DE) Die vorliegende Erfindung betrifft ein Verfahren zum Nachweis Epitop−spezifischer T−Zellen und von Zielepitopen reaktiver T−Zellen. Die vorliegende Erfindung betrifft ferner Vektoren, umfassend einen spezifisch in antigenpräsentierenden Zellen durch den epitop−spezifischen Kontakt mit einer T−Zelle induzierbaren ersten Promotor, eine mit diesem ersten Promotor funktionell verbundene Nukleinsäure kodierend ein Markergen, einen in antigenpräsentierenden Zellen konstitutiven zweiten Promotor, und eine mit diesem zweiten Promotor funktionell verbundene Nukleinsäure. Die vorliegende Erfindung betrifft ferner antigenpräsentierende Zellen, transduziert mit den erfindungsgemäßen Vektoren.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SC, SD, SE, SG, SI, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)