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1. (WO2003040397) ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASE-RESISTANT MOLECULAR BEACONS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2003/040397 International Application No.: PCT/US2002/034388
Publication Date: 15.05.2003 International Filing Date: 24.10.2002
IPC:
C12Q 1/68 (2006.01) ,C12Q 1/70 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
Applicants:
GORILLA GENOMICS, INC. [US/US]; 850 Marina Village Parkway Alameda, CA 94501, US (AllExceptUS)
BECKMAN, Kenneth, B. [US/US]; US (UsOnly)
LARSEN, Robert, D. [US/US]; US (UsOnly)
LEE, Kathleen, Y. [US/US]; US (UsOnly)
Inventors:
BECKMAN, Kenneth, B.; US
LARSEN, Robert, D.; US
LEE, Kathleen, Y.; US
Agent:
QUINE, Jonathan, Alan ; Quine Intellectual Property Law Group, P.C. P.O. Box 458 Alameda, CA 94501, US
Priority Data:
60/336,85130.10.2001US
60/346,26325.10.2001US
Title (EN) ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASE-RESISTANT MOLECULAR BEACONS
(FR) PCR ASYMETRIQUE AVEC UNE POLYMERASE NON NUCLEASIQUE OU DES BALISES MOLECULAIRES RESISTANT AUX NUCLEASES
Abstract:
(EN) The invention provides methods for performing combined asymmetric amplification (e.g. asymmetric PCR amplification) and detection of nucleic acid targets using molecular beacons to detect the products. Methods using a polymerase having reduced or eliminated 5' to 3' nuclease activity are provided, as are methods using nuclease-resistant molecular beacons. Asymmetric amplifications using nuclease-free polymerase or nucleease-resistant molecular beacons provide dramatic improvements in signal intensity detected as a result of molecular beacon binding to a target nucleic acid, e.g., during asymmetric PCR. Attendant compositions, systems, devices and kits are also features of the invention.
(FR) L'invention concerne des méthodes destinées à réaliser une amplification asymétrique combinée (telle qu'une amplification PCR asymétrique) et une détection de cibles d'acides nucléiques au moyen de balises moléculaires en vue de détecter des produits. Elle concerne des méthodes utilisant une polymérase présentant une activité nucléasique de 5' à 3' réduite ou nulle, ainsi que des méthodes utilisant des balises moléculaires résistant aux nucléases. Les amplifications asymétriques utilisant une polymérase non nucléasique ou des balises moléculaires résistant aux nucléases permettent d'améliorer considérablement l'intensité de signal détectée à la suite d'une liaison des balises moléculaires à un acide nucléique cible, notamment pendant une PCR asymétrique. L'invention concerne également des compositions, des systèmes, des dispositifs et des trousses associés.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1444365CA2462505AU2002363464