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1. (WO2003008636) UNIVERSAL METHOD AND COMPOSITION FOR THE RAPID LYSIS OF CELLS FOR THE RELEASE OF NUCLEIC ACIDS AND THEIR DETECTION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2003/008636    International Application No.:    PCT/CA2002/001088
Publication Date: 30.01.2003 International Filing Date: 19.07.2002
IPC:
C12Q 1/68 (2006.01)
Applicants: INFECTIO DIAGNOSTIC (I.D.I.) INC. [CA/CA]; 2050, Boul. René-Lévesque Ouest. 4ièm étage Sainte-Foy, Quebec G1V 2K8 (CA) (For All Designated States Except US).
MENARD, Christian [CA/CA]; (CA) (For US Only).
PICARD, François, J. [CA/CA]; (CA) (For US Only)
Inventors: MENARD, Christian; (CA).
PICARD, François, J.; (CA)
Agent: DUBUC, J.; Goudreau Gage Dubuc Stock Exchange Tower Suite 3400, 800 Place Victoria P.O. Box 242 Montreal, Quebec H4Z 1E9 (CA)
Priority Data:
60/306,163 19.07.2001 US
Title (EN) UNIVERSAL METHOD AND COMPOSITION FOR THE RAPID LYSIS OF CELLS FOR THE RELEASE OF NUCLEIC ACIDS AND THEIR DETECTION
(FR) PROCEDE UNIVERSEL ET COMPOSITION POUR LA LYSE RAPIDE DE CELLULES PERMETTANT LA LIBERATION D'ACIDES NUCLEIQUES ET LEUR DETECTION
Abstract: front page image
(EN)This invention describes a rapid (10 to 15 minutes), simple, flexible and efficient method of nucleic acids extraction for nucleic acid testing assays. This method has the following basic steps: i) mechanical cell lysis using solid particles in the presence of a chelating agent, followed by ii) controlling the presence and/or activity of NAT assays inhibitors. This method is applicable to various biological samples and universal for microorganisms, as one can use it to extract nucleic acids from test samples containing target viruses, bacteria, bacterial spores, fungi, parasites or other eukaryotic cells, including animal and human cells.
(FR)L'invention concerne un procédé rapide (10 à 15 minutes), simple, flexible et efficace d'extraction d'acides nucléiques pour des tests faisant intervenir des acides nucléiques (NAT). Ce procédé comprend principalement les étapes suivantes : i) lyse mécanique de cellules au moyen de particules solides en présence d'un agent chélateur et ii) contrôle de la présence et/ou de l'activité d'inhibiteurs de tests NAT. Ce procédé peut être appliqué à divers échantillons biologiques et il est universel pour les microorganismes, ledit procédé pouvant être mis en oeuvre pour extraire des acides nucléiques d'échantillons contenant des microorganismes cibles tels que des virus, des bactéries, des spores bactériennes, des champignons, des parasites ou des cellules eucaryotes, notamment des cellules animales et humaines.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)