WO2003008074 BUFFERS FOR ELECTROPHORESIS AND USE THEREOF

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Publication Number WO/2003/008074

Publication Date 30.01.2003

International Application No. PCT/US2002/023039

International Filing Date 19.07.2002

IPC

CPC

Applicants

APPLERA CORPORATION [US]/[US]

Inventors

HACKER, Kevin
VOSS, Karl

Agents

FRAZIER, Jeffery

Priority Data

09/909,649

19.07.2001

Publication Language English (en)

Filing Language English (EN)

Designated States

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Title

(EN) BUFFERS FOR ELECTROPHORESIS AND USE THEREOF

(FR) TAMPONS POUR ELECTROPHORESE ET UTILISATION CORRESPONDANTE

Abstract

(EN) Buffer compositions with or without sieving formulations are useful when used in connection with a capillary electrophoresis systeem as shown in Figure. The buffer composition can include Bis-Tris, TAPS and/or TAPSO and optionally a chelating agent such as EDTA. Methods of separating samples containing DNA or RNA with the capillary electrophoresis system are also described.

(FR) L'invention concerne des compositions de tampon avec ou sans tamisage des formulations, lesquelles sont utiles lorsqu'elles sont utilisées avec un système d'électrophorèse capillaire tel qu'illustré dans la figure 1. La composition de tampon peut contenir du Bis-Tris, du TAPS et/ou du TAPSO et éventuellement un agent chélatant tel que EDTA. L'invention concerne également des procédés destinés à séparer des échantillons contenant de l'ADN ou de l'ARN avec le système d'électrophorèse capillaire.

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27	Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26	by investigating electrochemical variables; by using electrolysis or electrophoresis
416	Systems
447	using electrophoresis

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27	Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26	by investigating electrochemical variables; by using electrolysis or electrophoresis
416	Systems
447	using electrophoresis

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27	Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26	by investigating electrochemical variables; by using electrolysis or electrophoresis
416	Systems
447	using electrophoresis
44704	Details; Accessories
44747	Composition of gel or of carrier mixture

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27	Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26	by investigating electrochemical variables; by using electrolysis or electrophoresis
416	Systems
447	using electrophoresis
44704	Details; Accessories
44747	Composition of gel or of carrier mixture

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1. WO2003008074 - BUFFERS FOR ELECTROPHORESIS AND USE THEREOF