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1. (WO2003006650) METHOD OF PURIFYING NUCLEIC ACID USING NONWOVEN FABRIC AND DETECTION METHOD
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2003/006650    International Application No.:    PCT/JP2002/006939
Publication Date: 23.01.2003 International Filing Date: 09.07.2002
Chapter 2 Demand Filed:    09.07.2002    
IPC:
C12N 15/10 (2006.01)
Applicants: ASAHI KASEI KABUSHIKI KAISHA [JP/JP]; 2-6, Dojimahama 1-chome, Kita-ku, Kita-ku, Osaka-shi, Osaka 530-8205 (JP) (For All Designated States Except US).
KANNO, Kimiyoshi [JP/JP]; (JP) (For US Only).
ODA, Naozumi [JP/JP]; (JP) (For US Only).
ARITOMI, Masaharu [JP/JP]; (JP) (For US Only).
SATO, Akiko [JP/JP]; (JP) (For US Only)
Inventors: KANNO, Kimiyoshi; (JP).
ODA, Naozumi; (JP).
ARITOMI, Masaharu; (JP).
SATO, Akiko; (JP)
Agent: HIRAKI, Yusuke; Toranomon No.5 Mori Building Third Floor 17-1, Toranomon 1-chome, Minato-ku, Tokyo 105-0001 (JP)
Priority Data:
2001-208514 09.07.2001 JP
2001-364878 29.11.2001 JP
Title (EN) METHOD OF PURIFYING NUCLEIC ACID USING NONWOVEN FABRIC AND DETECTION METHOD
(FR) PROCEDE DE PURIFICATION D'ACIDE NUCLEIQUE AU MOYEN D'UN NON-TISSE ET PROCEDE DE DETECTION
Abstract: front page image
(EN)A method of separating and purifying a nucleic acid from a sample containing cells such as blood or a liquid culture medium. In this method, a cell extract obtained by disrupting cell is adsorbed by a filter made of a nonwoven fabric and, after washing the filter, the nucleic acid is eluted therefrom. The nucleic acid may be eluted under alkaline conditions (pH 12 or higher). It is also possible to elute the nucleic acid by treating the nucleic acid adsorbed by the filer with active oxygen or using a surfactant. Nucleic acids separated and purified by the above method are usable in nucleic acid amplification or base sequence analysis techniques.
(FR)Procédé servant à séparer un acide nucléique d'un spécimen contenant des cellules, telles que du sang ou un milieu de culture liquide, et à purifier cet acide nucléique. Ce procédé consiste à effectuer l'adsorption d'un extrait cellulaire obtenu par dislocation cellulaire à l'aide d'un filtre en non-tissé et, après lavage de ce filtre, à en éluer l'acide nucléique. On peut éluer cet acide nucléique dans des conditions alcalines (pH12 ou supérieur). Il est également possible d'éluer l'acide nucléique par traitement de l'acide nucléique adsorbé par le filtre au moyen d'oxygène actif ou d'un tensioactif. On peut utiliser les acides nucléiques séparés et purifiés par l'intermédiaire de ce procédé dans des techniques d'analyse de séquences de base ou d'amplification d'acide nucléique.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)