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1. WO2002038809 - METHOD AND APPARATUS FOR DETECTING A MUTAITON IN A NUCLEIC ACID FRAGMENT IN A SAMPLE

Publication Number WO/2002/038809
Publication Date 16.05.2002
International Application No. PCT/NL2000/000769
International Filing Date 23.10.2000
Chapter 2 Demand Filed 23.05.2002
IPC
B01L 3/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B01L 7/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus; Heat insulating devices
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 27/447 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
B01L 2300/0838
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0832cylindrical, tube shaped
0838Capillaries
B01L 2300/1844
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
18Means for temperature control
1838using fluid heat transfer medium
1844using fans
B01L 2400/0421
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2400Moving or stopping fluids
04Moving fluids with specific forces or mechanical means
0403specific forces
0415electrical forces, e.g. electrokinetic
0421electrophoretic flow
B01L 3/5025
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5025for parallel transport of multiple samples
B01L 3/502753
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502753characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
B01L 7/525
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus
52with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
525with physical movement of samples between temperature zones
Applicants
  • INGENY HOLDING BV [NL]/[NL] (AllExceptUS)
  • DE VOS, Gerrit, Johannis [NL]/[NL] (UsOnly)
Inventors
  • DE VOS, Gerrit, Johannis
Agents
  • 'T JONG, Bastiaan, Jacobus
  • VAN SOMEREN, Petronella, Francisca, Hendrika, Maria
Priority Data
Publication Language English (EN)
Filing Language Dutch (NL)
Designated States
Title
(EN) METHOD AND APPARATUS FOR DETECTING A MUTAITON IN A NUCLEIC ACID FRAGMENT IN A SAMPLE
(FR) PROCEDE ET APPAREIL DE DETECTION DE MUTATION DANS UN FRAGMENT D'ACIDE NUCLEIQUE CONTENU DANS UN ECHANTILLON
Abstract
(EN)
The present invention refers to methods and devices for the detection of polymorphisms in a nucleic acid sample (e.g. blood, sperm, saliva, cells, ...). To enhance the efficiency and the reliability of the known methods (e.g. DGGE, SSCP and TGGE) the amplification process (e.g. PCR) preceding the actual detection step is performed in or on the polyacrylamide gel. Multiple gradients (of chemical denaturants, thermal denaturants and of porosity of the gel matrix) are used for the separation of DNA fragments, by zone electrophoresis on gel slabs or by capillary electrophoresis.
(FR)
Cette invention se rapporte à des procédés et à des dispositifs permettant de détecter les polymorphismes dans un échantillon d'acide nucléique (par exemple un échantillon de sang, de sperme, de salive, de cellules, etc). Pour améliorer l'efficacité et la fiabilité des procédés connus (tels que DGGE, SSCP et TGGE), le processus d'amplification (par exemple PCR) précédant la phase de détection effective est effectué dans ou sur le gel de polyacrylamide. De multiples gradients (de dénaturants chimiques, de dénaturants thermiques et de porosité de la matrice de gel) sont utilisés pour la séparation des fragments d'ADN, par électrophorèse de zone sur plaques de gel ou par électrophorèse capillaire.
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