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1. WO2002037967 - METHODS FOR GENERATING GENETICALLY ALTERED ANTIBODY-PRODUCING CELL LINES WITH IMPROVED ANTIBODY CHARACTERISTICS

Publication Number WO/2002/037967
Publication Date 16.05.2002
International Application No. PCT/US2000/030588
International Filing Date 07.11.2000
Chapter 2 Demand Filed 07.06.2002
IPC
C07K 16/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C07K 16/42 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
42against immunoglobulins (anti-idiotypic antibodies)
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
A01K 2217/05
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
2217Genetically modified animals
05Animals comprising random inserted nucleic acids (transgenic)
C07K 16/4291
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
42against immunoglobulins
4283against an allotypic or isotypic determinant on Ig
4291against IgE
C07K 2317/92
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2317Immunoglobulins specific features
90characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
C12N 15/1024
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
102Mutagenizing nucleic acids
1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
Applicants
  • MORPHOTEK INC. [US]/[US] (AllExceptUS)
  • NICOLAIDES, Nicholas, C. [US]/[US] (UsOnly)
  • GRASSO, Luigi [US]/[US] (UsOnly)
  • SASS, Philip, M. [US]/[US] (UsOnly)
Inventors
  • NICOLAIDES, Nicholas, C.
  • GRASSO, Luigi
  • SASS, Philip, M.
Agents
  • CALDWELL, John, W.
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR GENERATING GENETICALLY ALTERED ANTIBODY-PRODUCING CELL LINES WITH IMPROVED ANTIBODY CHARACTERISTICS
(FR) PROCEDES POUR GENERER DES LIGNEES CELLULAIRES PRODUISANT DES ANTICORPS GENETIQUEMENT MODIFIES PRESENTANT DES CARACTERISTIQUES AMELIOREES
Abstract
(EN)
Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production.
(FR)
Des allèles dominants négatifs de gènes humains de réparation de mésappariements peuvent être utilisés pour générer des cellules et des organismes hypermutables. En introduisant ces gènes dans des cellules et des animaux transgéniques, on peut produire de nouvelles lignées cellulaires et de nouvelles variétés animales présentant de nouvelles propriétés utiles avec une plus grande efficacité qu'en se basant sur le taux naturel de mutation. Ces procédés permettent de générer une diversité génétique à l'intérieur de gènes des immunoglobulines visant un antigène spécifique pour produire des anticorps modifiés présentant une activité biochimique accrue. Ces procédés permettent en outre de générer des cellules produisant des anticorps avec un niveau accru de production d'anticorps.
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