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1. WO2002037106 - METHODS FOR COMPARITIVE ANALYSIS OF CARBOHYDRATE POLYMERS

Publication Number WO/2002/037106
Publication Date 10.05.2002
International Application No. PCT/US2001/047064
International Filing Date 05.11.2001
Chapter 2 Demand Filed 30.05.2002
IPC
C12Q 1/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
34involving hydrolase
G01N 33/53 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
CPC
C12Q 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
G01N 33/5308
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
5308for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
Applicants
  • PROCOGNIA, LTD. [IL]/[IL] (AllExceptUS)
  • MARKMAN, Ofer [IL]/[IL] (UsOnly)
  • ROTHMAN, Chana [IL]/[IL] (UsOnly)
  • AMOR, Yehudit [IL]/[IL] (UsOnly)
  • ORON, Assaf [US]/[IL] (UsOnly)
  • OR, Einat [IL]/[IL] (UsOnly)
Inventors
  • MARKMAN, Ofer
  • ROTHMAN, Chana
  • AMOR, Yehudit
  • ORON, Assaf
  • OR, Einat
Agents
  • JOHNSON, David, E.
Priority Data
60/245,81703.11.2000US
60/245,88703.11.2000US
60/246,00603.11.2000US
60/246,00903.11.2000US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR COMPARITIVE ANALYSIS OF CARBOHYDRATE POLYMERS
(FR) PROCEDES D'ANALYSE COMPARATIVE DE POLYMERES A BASE D'HYDRATES DE CARBONE ET POLYMERES A BASE D'HYDRATES DE CARBONE AINSI IDENTIFIES
Abstract
(EN)
Disclosed is a method for characterizing a carbohydrate polymer by identifying at least two binding agents that bind to the carbohydrate polymer. Binding is preferably determined by contacting the carbohydrate polymer with substrate that contains a plurality of first saccharide-binding agents affixed at predetermined locations on the substrate. The carbohydrate polymer is allowed to contact the substrate under conditions that allow for formation of a first complex between the first saccharide-binding agent and the carbohydrate polymer. A second saccharide-binding agent, which preferably includes a label, is also contacted with the carbohydrate polymer under conditions that allow for formation of a second complex between the second binding agent and the first complex. Identification of the first and second binding agent allows for characterization of the polysaccharide.
(FR)
L'invention concerne un procédé de caractérisation de polymère à base d'hydrates de carbone reposant sur l'identification d'au moins deux agents de liaison liés au polymère. De préférence, la liaison est déterminée par contact entre le polymère et un substrat renfermant des premiers agents de liaison de saccharide fixés en des points préétablis du substrat. Le polymère entre en contact avec le substrat dans des conditions qui permettent la formation d'un premier complexe entre le premier agent de liaison de saccharide et le polymère. Un second agent de liaison de saccharide, comprenant de préférence un marqueur, entre également en contact avec le polymère dans des conditions qui permettent la formation d'un second complexe entre le second agent de liaison et le premier complexe. L'identification des premier et second agents de liaison permet de caractériser le polysaccharide.
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