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1. WO2002031523 - METHOD AND DEVICE FOR ANALYSING THE INTRACELLULAR CHEMICAL STATE OF LIVING CELLS BY NUCLEAR MAGNETIC RESONANCE

Publication Number WO/2002/031523
Publication Date 18.04.2002
International Application No. PCT/FR2001/003167
International Filing Date 12.10.2001
Chapter 2 Demand Filed 29.04.2002
IPC
G01N 1/42 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
42Low-temperature sample treatment, e.g. cryofixation
G01R 33/465 2006.01
GPHYSICS
01MEASURING; TESTING
RMEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
33Arrangements or instruments for measuring magnetic variables
20involving magnetic resonance
44using nuclear magnetic resonance
46NMR spectroscopy
465applied to biological material, e.g. in vitro testing
CPC
G01N 1/42
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation ; including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
42Low-temperature sample treatment, e.g. cryofixation
G01R 33/465
GPHYSICS
01MEASURING; TESTING
RMEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
33Arrangements or instruments for measuring magnetic variables
20involving magnetic resonance
44using nuclear magnetic resonance [NMR]
46NMR spectroscopy
465applied to biological material, e.g. in vitro testing
Y10T 436/24
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
24Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
Applicants
  • METABOLIC EXPLORER [FR]/[FR] (AllExceptUS)
  • BRUKER SA [FR]/[FR] (AllExceptUS)
  • GONZALEZ, Benjamin [FR]/[FR] (UsOnly)
  • PIOTTO, Martial [FR]/[FR] (UsOnly)
  • HUBER, Gaspard [FR]/[FR] (UsOnly)
Inventors
  • GONZALEZ, Benjamin
  • PIOTTO, Martial
  • HUBER, Gaspard
Agents
  • MARTIN, Jean-Jacques
Priority Data
00/1318313.10.2000FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) METHOD AND DEVICE FOR ANALYSING THE INTRACELLULAR CHEMICAL STATE OF LIVING CELLS BY NUCLEAR MAGNETIC RESONANCE
(FR) PROCEDE ET DISPOSITIF D'ANALYSE DE L'ETAT CHIMIQUE INTRACELLULAIRE DE CELLULES VIVANTES PAR RESONANCE MAGNETIQUE NUCLEAIRE
Abstract
(EN)
The invention concerns a method for analysing the intracellular chemical state of living cells by nuclear magnetic resonance (NMR). Said method comprises a step which consists in preparing the cell sample and a step which consists in an NMR analysis of the sample, both steps being carried out at very low temperature so as to freeze the biochemical state of the living cells to obtain specific and accurate reproducible measurements of the cellular functioning in given conditions. The invention also concerns the device coupled to the NMR spectrometer for implementing the method of analysis.
(FR)
La présente invention concerne un procédé d'analyse de l'état chimique intracellulaire de cellules vivantes par résonance magnétique nucléaire (RMN). Ce procédé comprend une étape de préparation de l'échantillon de cellules et une étape d'analyse de l'échantillon par RMN réalisées à très basse température afin de figer l'état biochimique des cellules vivantes de façon à avoir des mesures reproductibles spécifiques et justes du fonctionnement cellulaire dans des conditions données. L'invention concerne également le dispositif couplé au spectromètre RMN pour mettre en oeuvre le procédé d'analyse.
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