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1. (WO2002030962) METAL BINDING PROTEINS, RECOMBINANT HOST CELLS AND METHODS

Pub. No.:    WO/2002/030962    International Application No.:    PCT/US2001/031819
Publication Date: Apr 18, 2002 International Filing Date: Oct 12, 2001
IPC: C07K 14/25
Applicants: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
Inventors: SUMMERS, Anne, O.
CAGUIAT, Jonathan, J.
Title: METAL BINDING PROTEINS, RECOMBINANT HOST CELLS AND METHODS
Abstract:
The present disclosure provides artificial heave metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metal taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.