WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2002029034) EXBB-EXBD-TONB GENE CLUSTER OF PSEUDOMONAS PUTIDA DOT-T1E CONFERRING TOLERANCE TO AROMATIC COMPOUNDS AND RESISTANCE TO ANTIBIOTICS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2002/029034    International Application No.:    PCT/US2001/031180
Publication Date: 11.04.2002 International Filing Date: 28.09.2001
Chapter 2 Demand Filed:    10.04.2002    
IPC:
C07K 14/21 (2006.01), C12N 15/52 (2006.01), C12P 7/42 (2006.01)
Applicants: NORTH CAROLINA STATE UNIVERSITY [US/US]; 2401 Research Drive Campus Box 8210 Raleigh, NC 27695-8210 (US)
Inventors: RAMOS, Juan, L.; (ES).
BEN-BASSAT, Arie; (US).
GODOY, Patricia; (US).
RAMOS-GONZALES, M., Isabel; (ES).
DUQUE, Estrella; (US)
Agent: SIBLEY, Kenneth, D.; Meyers Bigel Sibley & Sajovec, P.O. Box 37428, Raleigh, NC 27627 (US)
Priority Data:
50/236,879 30.09.2000 US
Title (EN) EXBB-EXBD-TONB GENE CLUSTER OF PSEUDOMONAS PUTIDA DOT-T1E CONFERRING TOLERANCE TO AROMATIC COMPOUNDS AND RESISTANCE TO ANTIBIOTICS
(FR) PROCEDES DE PRODUCTION DE P-HYDROXYBENZOATE DANS DES BACTERIES
Abstract: front page image
(EN)This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production.
(FR)L'invention concerne l'isolement d'un nouveau tonB operon du Pseudomonas putida. Ces gènes sont utilisés pour rendre les cellules plus sensibles aux antibiotiques, au toluène, au pHBA, aux composés aromatiques, aux parabènes ainsi qu'aux aminoacides aromatiques, après inactivation avec des allèles mutants spécifiques, ou plus tolérantes à ces composés, après surexpression avec un vecteur d'expression approprié. Ces observations sont importantes dans le domaine de la médecine, de la biotechnologie et de la biocatalyse. L'invention spécifie en outre un écran permettant d'identifier des gènes tolérants au pHBA, et des souches à tolérance significative au pHBA ont été identifiées. Ces souches sont importantes pour la production de pHBA.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)