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1. (WO2002026956) CALCIUM FREE SUBTILISIN MUTANTS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2002/026956    International Application No.:    PCT/US2001/030092
Publication Date: 04.04.2002 International Filing Date: 26.09.2001
Chapter 2 Demand Filed:    02.04.2002    
IPC:
C11D 3/386 (2006.01), C12N 9/54 (2006.01)
Applicants: UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE [US/US]; Office of Research Admin./Tech. Dev., Suite 200, 701 E. Pratt Street, Baltimore, MD 21202 (US)
Inventors: BRYAN, Philip, N.; (US)
Agent: HULTQUIST, Steven, J.; Intellectual Property/Technology Law, P.O. Box 14329, Research Triangle Park, NC 27709 (US)
Priority Data:
09/672,105 29.09.2000 US
Title (EN) CALCIUM FREE SUBTILISIN MUTANTS
(FR) MUTANTS DE SUBTILISINE SANS CALCIUM
Abstract: front page image
(EN)Novel calcium free subtilisin mutants are taught, in particular subtilisins which have been mutated to eliminate amino acids 75-83 and part or all of amino acids 1-22 (the N-terminal region) and which retain enzymatic activity and stability. Recombinant methods for producing the same and recombinant DNA encoding for such subtilisin mutants are also provided.
(FR)L'invention a trait à de nouveaux mutants de subtilisine sans calcium, notamment des subtilisines ayant été mutées pour éliminer les acides aminés 75-83 ainsi qu'une partie ou la totalité des acides aminés 1-22 (la région N-terminale) et lesquels conservent une activité et une stabilité enzymatiques. L'invention a également trait à des méthodes de recombinaison permettant leur production ainsi qu'au codage d'ADN recombiné pour ces mutants de subtilisine.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)