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1. WO2002020831 - METHOD FOR MEASUREMENT OF THE ACTIVITY OF NO-SYNTHASE

Publication Number WO/2002/020831
Publication Date 14.03.2002
International Application No. PCT/EP2001/010151
International Filing Date 04.09.2001
Chapter 2 Demand Filed 27.03.2002
IPC
C12Q 1/26 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
26involving oxidoreductase
CPC
C12Q 1/26
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
26involving oxidoreductase
G01N 2333/90245
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
90Enzymes; Proenzymes
902Oxidoreductases (1.)
90245acting on paired donors with incorporation of molecular oxygen (1.14)
G01N 2500/02
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2500Screening for compounds of potential therapeutic value
02Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
Applicants
  • GRÜNENTHAL GMBH [DE]/[DE] (AE, AG, AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BY, BZ, CA, CF, CG, CH, CI, CM, CN, CO, CR, CU, CY, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GW, HR, HU, ID, IE, IL, IN, IS, IT, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MC, MD, MG, MK, ML, MN, MR, MW, MX, MZ, NE, NL, NO, NZ, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, SN, SZ, TD, TG, TJ, TM, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZW)
  • HENNIES, Hagen-Heinrich [--]/[DE] (UsOnly)
  • SUNDERMANN, Bernd [DE]/[DE] (UsOnly)
Inventors
  • HENNIES, Hagen-Heinrich
  • SUNDERMANN, Bernd
Priority Data
100 43 845.806.09.2000DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) VERFAHREN ZUR MESSUNG DER AKTIVITÄT DER NO-SYNTHASE
(EN) METHOD FOR MEASUREMENT OF THE ACTIVITY OF NO-SYNTHASE
(FR) PROCEDE POUR MESURER L'ACTIVITE DE LA NO SYNTHASE
Abstract
(DE)
Die Erfindung betrifft ein Verfahren zur Messung der Aktivität der NO-Synthase, ein entsprechendes Verfahren zur Identifizierung von NO-Synthase-Modulatoren, insbesondere zum Einsatz in einem HTS (high throughput screening), und die Verwendung der entsprechenden Kationenaustausch-Filterplattenmembranen.
(EN)
The invention relates to a method for measuring the activity of NO-synthase, a corresponding method for identification of NO-synthase modulators, in particular for application in a HTS (High Throughput Screening) and use of the corresponding cation-exchange filter-plate membranes.
(FR)
L'invention concerne un procédé pour mesurer l'activité de la NO synthase, un procédé correspondant pour l'identification de modulateurs de NO synthase, en particulier pour application dans une sélection à haut rendement (HTS), et l'utilisation des membranes à plaque filtrante échangeuses de cations correspondantes.
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