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1. WO2002020829 - CULTURE MEDIUM AND METHOD FOR IDENTIFYING GRAM-NEGATIVE MICROORGANISMS

Publication Number WO/2002/020829
Publication Date 14.03.2002
International Application No. PCT/CU2001/000006
International Filing Date 24.08.2001
Chapter 2 Demand Filed 08.04.2002
IPC
C12Q 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
CPC
A61P 29/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
29Non-central analgesic, antipyretic or anti-inflammatory agents, e.g antirheumatic agents; Non-steroidal anti-inflammatory drugs (NSAIDs)
C12Q 1/045
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
045Culture media therefor
Y02A 50/30
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
50in human health protection, e.g. against extreme weather
30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Applicants
  • CENTRO NACIONAL DE BIOPREPARADOS [CU]/[CU] (AllExceptUS)
  • RODRIGUEZ MARTINEZ, Claudio [CU]/[CU] (UsOnly)
  • QUESADA MUÑIZ, Vivian de Jesus [CU]/[CU] (UsOnly)
  • ZHURBENKO, Raisa [RU]/[CU] (UsOnly)
Inventors
  • RODRIGUEZ MARTINEZ, Claudio
  • QUESADA MUÑIZ, Vivian de Jesus
  • ZHURBENKO, Raisa
Agents
  • MORENO SAMPER, Olga Lidia
Priority Data
195/200007.09.2000CU
Publication Language Spanish (ES)
Filing Language Spanish (ES)
Designated States
Title
(EN) CULTURE MEDIUM AND METHOD FOR IDENTIFYING GRAM-NEGATIVE MICROORGANISMS
(ES) MEDIO DE CULTIVO Y MÉTODO PARA LA IDENTIFICACIÓN DE MICROORGANISMOS GRAM-NEGATIVOS
(FR) MILIEU DE CULTURE ET METHODE DESTINES A L'IDENTIFICATION DE MICRO-ORGANISMES GRAM-NEGATIFS
Abstract
(EN)
The invention relates to a novel culture medium and a method for the identification of gram-negative microorganisms based on the differentiation of said microorganisms by the appearance of 10 different colors in the colonies, which may be regular or irregular, and halos of at least 5 different colors and sizes. Said medium comprises a mixture of components favoring the appearance of halos of different colors and sizes and consists of siliceous earth, skim milk, starches and activated carbon. The medium according to the invention also comprises a mixture of nutritional bases, substances ensuring the appearance of different colorations in the colonies, substances ensuring inhibition of gram-positive microorganisms and substances providing the necessary solid matrix for the growth and development of the colonies.
(ES)
La invención consiste en un nuevo medio de cultivo y en un método para la identificación de microorganismos Gram negativos basados en la diferenciación de éstos por la aparición de al menos 10 colores diferentes de las colonias, que pueden ser regulares o irregulares, y de halos de al menos 5 diferentes coloraciones y tamaños. Dicho medio comprende una mezcla de componentes que propician la aparición de halos de colores y tamaños distintos, constituida por tierra silícea, leche descremada, almidones y carbón activado. El medio de la invención comprende además una mezcla de bases nutritivas, sustancias que garantizan la aparición de diferentes coloraciones en las colonias, sustancias que garantizan la inhibición de los microorganismos Gram positivos y sustancias que proporcionan la matriz sólida necesaria para el crecimiento y desarrollo de las colonias.
(FR)
L'invention concerne un milieu de culture et une méthode destinés à l'identification d'organismes gram-négatifs se basant sur la différenciation de ces derniers par apparition d'au moins 10 couleurs différentes des colonies, qui peuvent être régulières ou irrégulières, et de halos d'au moins 5 différentes colorations et dimensions. Ce milieu comprend un mélange de composants qui favorisent l'apparition de halos de différentes couleurs et dimensions, ce mélange étant constitué de terre siliceuse, de lait écrémé, d'amidons et de charbon activé. En outre, ce milieu comprend un mélange de bases nutritives, de substances qui garantissent l'apparition de différentes colorations dans les colonies, de substances qui garantissent l'inhibition des micro-organismes gram-positifs et de substances qui fournissent la matrice solide nécessaire à la croissance et au développement des colonies.
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