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1. WO2002020730 - MANGANESE LIPOXYGENASE

Publication Number WO/2002/020730
Publication Date 14.03.2002
International Application No. PCT/DK2001/000574
International Filing Date 05.09.2001
Chapter 2 Demand Filed 20.02.2002
IPC
A21D 10/00 2006.01
AHUMAN NECESSITIES
21BAKING; EQUIPMENT FOR MAKING OR PROCESSING DOUGHS; DOUGHS FOR BAKING
DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
10Batters, dough or mixtures before baking
A21D 13/00 2006.01
AHUMAN NECESSITIES
21BAKING; EQUIPMENT FOR MAKING OR PROCESSING DOUGHS; DOUGHS FOR BAKING
DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
13Finished or partly finished bakery products
C11D 1/22 2006.01
CCHEMISTRY; METALLURGY
11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
1Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
02Anionic compounds
12Sulfonic acids or sulfuric acid esters; Salts thereof
22derived from aromatic compounds
C11D 3/386 2006.01
CCHEMISTRY; METALLURGY
11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
3Other compounding ingredients of detergent compositions covered in group C11D1/101
16Organic compounds
38Products with no well-defined composition
386Preparations containing enzymes
C12N 1/15 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
15modified by introduction of foreign genetic material
C12N 1/19 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
CPC
C12N 9/0069
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0069acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Applicants
  • NOVOZYMES A/S [DK]/[DK] (AllExceptUS)
  • SUGIO, Akiko [JP]/[JP] (UsOnly)
  • TAKAGI, Shinobu [JP]/[JP] (UsOnly)
  • CHRISTENSEN, Søren [DK]/[DK] (UsOnly)
  • ØSTERGAARD, Lars [DK]/[DK] (UsOnly)
  • OLIW, Ernst [SE]/[SE] (UsOnly)
Inventors
  • SUGIO, Akiko
  • TAKAGI, Shinobu
  • CHRISTENSEN, Søren
  • ØSTERGAARD, Lars
  • OLIW, Ernst
Common Representative
  • NOVOZYMES A/S
Priority Data
0004790-222.12.2000SE
60/272,60401.03.2001US
PA 2000 0132005.09.2000DK
PA 2001 0032227.02.2001DK
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MANGANESE LIPOXYGENASE
(FR) LIPOXYGENASE
Abstract
(EN)
The invention provides sequence information of a microbial protein having lipoxy-genase activity and a method of producing the protein by recombinant DNA technology. More specifically, the inventors have isolated a gene encoding a lipoxygenase from Gaeu-mannomyces graminis, cloned it into an E. coli strain and sequenced it. A comparison shows less than 25 % identity to known lipoxygenase sequences, the closest being human 15S li-poxygenase. The inventors have expressed the lipoxygenase recombinantly and found that the recombinant lipoxygenase is glycosylated.
(FR)
L'invention concerne des informations relatives à la séquence d'une protéine microbienne exerçant une activité de lipoxygénase et concerne un procédé de production de la protéine par génie génétique. Plus précisément, les inventeurs ont isolé un gène codant une lipoxygénase issue de Gaeumannomyces graminis, puis l'ont cloné dans une souche E.coli et l'ont séquencé. Une comparaison fait apparaître moins de 25 % d'identité avec les séquences de lipoxygénase connues, la plus proche étant la lipoxygénase humaine 15S. Les inventeurs ont exprimé la lipoxygénase par recombinaison et ont découvert que la lipoxygénase recombinée est glycosylée.
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