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1. WO2002019960 - PURIFICATION, CHARACTERIZATION AND USE OF PROTECTIVE ANTIGENIC STRUCTURES AGAINST TRYPANOSOMES AND RELATED PARASITES

Considered void:  10.12.2001
Publication Number WO/2002/019960
Publication Date 14.03.2002
International Application No. PCT/BE2001/000146
International Filing Date 06.09.2001
IPC
A61K 39/005 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
002Protozoa antigens
005Trypanosoma antigens
C07K 16/20 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
20from protozoa
H04L 29/06 2006.01
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
LTRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
29Arrangements, apparatus, circuits or systems, not covered by a single one of groups H04L1/-H04L27/136
02Communication control; Communication processing
06characterised by a protocol
H04L 29/08 2006.01
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
LTRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
29Arrangements, apparatus, circuits or systems, not covered by a single one of groups H04L1/-H04L27/136
02Communication control; Communication processing
06characterised by a protocol
08Transmission control procedure, e.g. data link level control procedure
CPC
A61K 2039/505
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
505comprising antibodies
A61K 39/005
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39Medicinal preparations containing antigens or antibodies
002Protozoa antigens
005Trypanosoma antigens
C07K 16/20
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
18against material from animals or humans
20from protozoa
H04L 29/06027
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
LTRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
29Arrangements, apparatus, circuits or systems, not covered by a single one of groups H04L1/00 - H04L27/00
02Communication control
06characterised by a protocol
0602Protocols characterised by their application
06027Protocols for multimedia communication
H04L 67/00
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
LTRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
67Network-specific arrangements or communication protocols supporting networked applications
H04L 69/16
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
LTRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
69Application independent communication protocol aspects or techniques in packet data networks
16Transmission control protocol/internet protocol [TCP/IP] or user datagram protocol [UDP]
Applicants
  • UNIVERSITE LIBRE DE BRUXELLES [BE]/[BE] (AllExceptUS)
  • BLACK, Samuel, J. [US]/[US] (AllExceptUS)
  • MURPHY, Noel [US]/[KE] (AllExceptUS)
  • PEARSON, Terry [CA]/[CA] (AllExceptUS)
  • PAYS, Etienne [BE]/[BE] (UsOnly)
  • NOLAN, Derek [IE]/[BE] (UsOnly)
Inventors
  • PAYS, Etienne
  • NOLAN, Derek
Agents
  • VAN MALDEREN, Eric
Priority Data
60/231,83906.09.2000US
null16.10.2000US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PURIFICATION, CHARACTERIZATION AND USE OF PROTECTIVE ANTIGENIC STRUCTURES AGAINST TRYPANOSOMES AND RELATED PARASITES
(FR) PURIFICATION, CARACTERISATION ET UTILISATION DE STRUCTURES ANTIGENIQUES PROTECTRICES CONTRE DES TRYPANOSOMES ET DES PARASITES APPARENTES
Abstract
(EN)
The present invention is related to a method for the recovering and the characterization of a surface antigenic structure of a trypanosome flagellar pocket (fp), characterized in that it comprises the following steps:- purifying membrane glycoproteins located on the surface of the flagellar pocket (fp) of said trypanosome, preferably glycoproteins containing linear poly-N-acetyllactosamine (pNAL), from a biological extract comprising said trypanosome,- deglycosylating said glycoproteins into proteins,- obtaining hybridomas secreting monoclonal antibodies directed against said proteins, - screening the resulting hybridomas for selecting a positive hybridoma which secretes an antibody able to inhibit the trypanosome growth (trypanosome activity in vitro), - obtaining said antibody from said positive hybridoma, - selecting and purifying an antigenic structure among the proteins of the trypanosome flagellar pocket (fp) by binding said proteins to said monoclonal antibody, and- possibly identifying the amino acid sequence of said antigenic structure and possibly the nucleotide sequence encoding said antigenic structure.
(FR)
L'invention concerne un procédé de récupération et de caractérisation d'une structure antigénique de surface d'une poche flagellaire de trypanosome (fp), caractérisé en ce qu'il consiste à purifier des glycoprotéines de membrane situées sur la surface de la poche flagellaire (fp) du trypanosome, de préférence des glycoprotéines contenant une poly-N-acétyllactosamine linéaire (pNAL), à partir d'un extrait biologique contenant le trypanosome, à déglycosyler ces glycoprotéines en protéines, à obtenir des hybridomes sécrétant des anticorps monoclonaux dirigés centre ces protéines, à cribler les hybridomes résultants afin de sélectionner un hybridome positif qui secrète un anticorps capable d'inhiber la croissance du trypanosome (activité du trypanosome in vitro), à obtenir cet anticorps à partir de cet hybridome, à sélectionner et à purifier une structure antigénique parmi les protéines de la poche flagellaire du trypanosome (fp) par liaison de ces protéines à l'anticorps monoclonal, et à éventuellement identifier la séquence des acides aminés de la structure antigénique ainsi que la séquence nucléotidique codant pour cette structure antigénique.
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