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1. WO2002018952 - BIOSENSOR ASSAY DEVICE AND METHOD

Publication Number WO/2002/018952
Publication Date 07.03.2002
International Application No. PCT/CA2001/001246
International Filing Date 30.08.2001
Chapter 2 Demand Filed 18.03.2002
IPC
G01N 33/543 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
CPC
G01N 33/54373
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54366Apparatus specially adapted for solid-phase testing
54373involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
G01N 33/5438
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
54366Apparatus specially adapted for solid-phase testing
54373involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
5438Electrodes
Applicants
  • HELIX BIOPHARMA CORPORATION [CA]/[CA] (AllExceptUS)
  • WONG, Wah, Y. [CA]/[CA] (UsOnly)
  • CHAO, Heman [CA]/[CA] (UsOnly)
  • McELROY, Jerry [CA]/[CA] (UsOnly)
  • SEGAL, Donald [CA]/[CA] (UsOnly)
Inventors
  • WONG, Wah, Y.
  • CHAO, Heman
  • McELROY, Jerry
  • SEGAL, Donald
Agents
  • ERRATT, Judy, A.
Priority Data
09/654,19101.09.2000US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BIOSENSOR ASSAY DEVICE AND METHOD
(FR) DISPOSITIF ET METHODE D'ANALYSE UTILISANT UN BIOCAPTEUR
Abstract
(EN)
A diagnostic method and device for use in detecting or quantitating an analyte present in a liquid sample. The method includes reacting an analyte-containing sample with reagents capable of generating a first coil-forming peptide in solution form. This peptide is then contacted with a biosensor whose detection surface has surface-bound molecules of a second, oppositely charged coil-forming peptide, under conditions effective to form a stable $g(a)-helical coiled-coil heterodimer on the detection surface. The formation of the coiled-coil heterodimer produces a measurable change in biosensor signal, when is measured to detect the presence of or quantitate the amount of analyte in a sample.
(FR)
L'invention concerne une méthode et un dispositif diagnostique destinés à être utilisés pour la détection et la quantification d'un analyte présent dans un échantillon liquide. Cette méthode consiste à faire réagir un échantillon contenant l'analyte avec des réactifs capables de générer un premier peptide apte à former des structures en hélice dans une solution. Ce peptide est alors mis en contact avec un biocapteur dont la surface de détection comprend des molécules fixées d'un second peptide apte à former une structure une hélice, à charge opposée, dans des conditions permettant la formation d'un hétérodimère à structure en superhélice hélicoïdal alpha sur la surface de détection. La formation de cet hétérodimère produit un changement dans le signal du biocapteur pouvant être mesuré, ce changement étant mesuré pour détecter la présence ou pour quantifier l'analyte situé dans l'échantillon.
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