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1. WO2002018627 - METHOD OF EXAMINING DISEASES WITH INBORN ERRORS OF METABOLISM AND EXAMINATION APPARATUS THEREFOR

Publication Number WO/2002/018627
Publication Date 07.03.2002
International Application No. PCT/JP2000/005788
International Filing Date 28.08.2000
Chapter 2 Demand Filed 22.02.2002
IPC
G01N 33/52 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
G01N 33/66 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
66involving blood sugars, e.g. galactose
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/52
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper ; and including single- and multilayer analytical elements
G01N 33/66
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
66involving blood sugars, e.g. galactose
G01N 33/6812
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6806Determination of free amino acids
6812Assays for specific amino acids
Applicants
  • SAPPORO IMMUNO DIAGNOSTIC LABORATORY [JP]/[JP] (AllExceptUS)
  • YOKOYAMA, Toru [JP]/[JP] (UsOnly)
  • SHINOZUKA, Naoki [JP]/[JP] (UsOnly)
  • NAKAMURA, Kenji [JP]/[JP] (UsOnly)
Inventors
  • YOKOYAMA, Toru
  • SHINOZUKA, Naoki
  • NAKAMURA, Kenji
Agents
  • SHAMOTO, Ichio
Priority Data
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD OF EXAMINING DISEASES WITH INBORN ERRORS OF METABOLISM AND EXAMINATION APPARATUS THEREFOR
(FR) PROCEDE D'EXAMEN DE MALADIES A ERREURS INNEES DU METABOLISME, ET APPAREIL D'EXAMEN CONCU A CET EFFET
Abstract
(EN)
A method of conveniently and quickly quantitating subject substances which comprises using as reaction reagents at least dehydrogenases specific respectively to branched amino acids containing D-galactose and L-leucine and phenylalanine, which are the substances contained in biological samples to be assayed, coenzymes, an electron mediator and a tetrazolium salt and detecting formazan, which has been finally formed depending on the concentrations of the subject substances by the enzymatic reactions and oxidation reactions of the biological samples with the reaction reagents, by using both or one of an optical detection procedure and an electrochemical detection procedure, thereby embodying a convenient and quick examination of three diseases with inborn errors of metabolism, i.e., galactosemia, maple syrup urine disease and phenylketonuria.
(FR)
L'invention concerne un procédé de quantification pratique et rapide de substances, consistant à utiliser, en tant que réactifs de réaction, au moins des déshydrogénases spécifiques d'acides aminés ramifiés contenant D-galactose et L-leucine et phénylanaline (qui sont les substances sont contenues dans des échantillons biologiques à analyser), des coenzymes, un médiateur de transfert d'électrons, ainsi qu'un sel de tétrazolium, puis à détecter le formazan, lequel s'est finalement formé en fonction des concentrations desdites substances, par l'intermédiaire des réactions enzymatiques et des réactions d'oxydation des échantillons biologiques, avec les réactifs de réaction; cette détection s'effectue par utilisation de deux procédures de détection, optique et électrochimique, ou par utilisation d'une seule des deux, et elle constitue ainsi un moyen de détection pratique et rapide de trois maladies comportant des erreurs innées du métabolisme, à savoir, la galactosémie, la maladie des urines à odeur de sirop d'érable, et la phénylcétonurie.
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