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1. WO2002018428 - SEQUENCES WHICH CODE FOR THE SIGE GENE OF CORYNEBACTERIUM GLUTAMICUM

Publication Number WO/2002/018428
Publication Date 07.03.2002
International Application No. PCT/EP2001/008146
International Filing Date 14.07.2001
Chapter 2 Demand Filed 27.03.2002
IPC
C07K 14/34 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
34from Corynebacterium (G)
C12P 13/08 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta-amino acids
08Lysine; Diaminopimelic acid; Threonine; Valine
CPC
C07K 14/34
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
34from Corynebacterium (G)
C12P 13/08
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta- amino acids
08Lysine; Diaminopimelic acid; Threonine; Valine
C12R 1/15
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RPROCESSES USING MICROORGANISMS
1Processes using microorganisms
01using bacteria or actinomycetales
15Corynebacterium
Applicants
  • DEGUSSA AG [DE]/[DE]
Inventors
  • MÖCKEL, Bettina
  • HERMANN, Thomas
  • FARWICK, Mike
  • BINDER, Michael
  • PFEFFERLE, Walter
Priority Data
100 43 336.702.09.2000DE
101 26 422.431.05.2001DE
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SEQUENCES WHICH CODE FOR THE SIGE GENE OF CORYNEBACTERIUM GLUTAMICUM
(FR) SEQUENCES CODANT LE GENE SIGE
Abstract
(EN)
The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
(FR)
L'invention concerne un polynucléotide isolé comprenant une séquence polynucléotidique choisie dans le groupe comprenant: a) un polynucléotide identique au moins à 70 % à un polynucléotide codant un polypeptide contenant la séquence d'acides aminés SEQ ID No. 2; b) un polynucléotide codant pour un polypeptide contenant une séquence d'acides aminés identique au moins à 70 % à la séquence d'acides aminés SEQ ID No. 2; c) un polynucléotide complémentaire des polynucléotides susmentionnés aux points a) ou b); et d) un polynucléotide comprenant au moins 15 nucléotides successifs de la séquence polynucléotidique décrite aux points a), b) ou c). L'invention concerne également un procédé de préparation par fermentation d'acides aminés L à l'aide de bactéries corynéformes dans lesquelles au moins le gène sigE est présent sous sa forme renforcée, ainsi que l'utilisation de polynucléotides comprenant les séquences selon l'invention comme sondes d'hybridation.
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