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1. WO2002006497 - TRANSPLASTOMIC PLANTS

Publication Number WO/2002/006497
Publication Date 24.01.2002
International Application No. PCT/EP2001/008132
International Filing Date 13.07.2001
Chapter 2 Demand Filed 12.02.2002
IPC
C07K 14/57 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
52Cytokines; Lymphokines; Interferons
555Interferons (IFN)
57IFN-gamma
C12N 15/62 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
62DNA sequences coding for fusion proteins
C12N 15/82 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C07K 14/57
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
52Cytokines; Lymphokines; Interferons
555Interferons [IFN]
57IFN-gamma
C07K 2319/21
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
20containing a tag with affinity for a non-protein ligand
21containing a His-tag
C07K 2319/50
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
50containing protease site
C07K 2319/61
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
61containing an enzyme fusion for detection (lacZ, luciferase)
C07K 2319/75
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
70containing domain for protein-protein interaction
74containing a fusion for binding to a cell surface receptor
75containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
C07K 2319/95
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
95containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
Applicants
  • INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY [IT]/[IT] (AllExceptUS)
  • REDDY, Vanga, Siva [IN]/[IN] (UsOnly)
  • SADHU, Leelavathi [IN]/[IN] (UsOnly)
Inventors
  • REDDY, Vanga, Siva
  • SADHU, Leelavathi
Agents
  • WOODS, Geoffrey, Corlett
Priority Data
0017397.114.07.2000GB
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TRANSPLASTOMIC PLANTS
(FR) PLANTES TRANSPLASTOMIQUES
Abstract
(EN) The present invention relates to a method of producing a protein of interest comprising allowing a polynucleotide fusion construct to be expressed in a plastid thereby to produce a fusion protein comprising the protein of interest, wherein the fusion construct comprises a polynucleotide coding sequence of the protein of interest operably linked to a polynucleotide coding sequence of a fusion protein partner and wherein the fusion protein thus expressed has a greater half-life than the individually expressed protein of interest, is resistant to internal cleavage in vivo and comprises a cleavage site.
(FR) L'invention porte sur un procédé de production d'une protéine d'intérêt consistant à faire exprimer un polynucléotide produit d'assemblage de fusion dans un plastide de manière à produire une protéine de fusion consistant dans la protéine d'intérêt. Le produit d'assemblage de fusion de la protéine d'intérêt comporte une séquence de codage de polynucléotide de la protéine d'intérêt fonctionnellement liée à la séquence de codage de polynucléotide d'une protéine de fusion partenaire. La protéine de fusion ainsi exprimée: a une demi-vie supérieure à celle d'une protéine d'intérêt exprimée individuellement, est résistante aux clivages internes in vivo, et comporte un site de clivage.
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