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1. WO2002004956 - METHOD OF IDENTIFYING CONFORMATION-SENSITIVE BINDING PEPTIDES AND USES THEREOF

Publication Number WO/2002/004956
Publication Date 17.01.2002
International Application No. PCT/US2001/021867
International Filing Date 11.07.2001
Chapter 2 Demand Filed 11.02.2002
IPC
G01N 33/566 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
566using specific carrier or receptor proteins as ligand binding reagent
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
H01S 5/026 2006.1
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
SDEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
5Semiconductor lasers
02Structural details or components not essential to laser action
026Monolithically integrated components, e.g. waveguides, monitoring photo-detectors or drivers
H01S 5/042 2006.1
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
SDEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
5Semiconductor lasers
04Processes or apparatus for excitation, e.g. pumping
042Electrical excitation
H01S 5/0625 2006.1
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
SDEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
5Semiconductor lasers
06Arrangements for controlling the laser output parameters, e.g. by operating on the active medium
062by varying the potential of the electrodes
0625in multi-section lasers
H01S 5/12 2006.1
HELECTRICITY
01BASIC ELECTRIC ELEMENTS
SDEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
5Semiconductor lasers
10Construction or shape of the optical resonator
12the resonator having a periodic structure, e.g. in distributed feed-back  lasers
CPC
B82Y 20/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
20Nanooptics, e.g. quantum optics or photonic crystals
C40B 30/04
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
30Methods of screening libraries
04by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
G01N 2500/10
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2500Screening for compounds of potential therapeutic value
10involving cells
G01N 33/566
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
566using specific carrier or receptor proteins as ligand binding reagents ; where possible specific carrier or receptor proteins are classified with their target compounds
G01N 33/6845
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6845Methods of identifying protein-protein interactions in protein mixtures
H04B 10/2914
HELECTRICITY
04ELECTRIC COMMUNICATION TECHNIQUE
BTRANSMISSION
10Transmission systems employing electromagnetic waves other than radio-waves, e.g. infrared, visible or ultraviolet light, or employing corpuscular radiation, e.g. quantum communication
29Repeaters
291in which processing or amplification is carried out without conversion of the main signal from optical form
2912characterised by the medium used for amplification or processing
2914using lumped semiconductor optical amplifiers [SOA]
Applicants
  • KARO BIO USA, INC. [US]/[US] (AllExceptUS)
  • FOWLKES, Dana, M. [US]/[US] (UsOnly)
  • BARNETT, Thomas, R. [US]/[US] (UsOnly)
  • BUEHRER, Benjamin [US]/[US] (UsOnly)
Inventors
  • FOWLKES, Dana, M.
  • BARNETT, Thomas, R.
  • BUEHRER, Benjamin
Agents
  • BROWDY AND NEIMARK, P.L.L.C.
Priority Data
09/614,86512.07.2000US
09/860,68821.05.2001US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF IDENTIFYING CONFORMATION-SENSITIVE BINDING PEPTIDES AND USES THEREOF
(FR) PROCEDE D'IDENTIFICATION DE PEPTIDES DE LIAISON SENSIBLES A LA CONFORMATION ET UTILISATIONS CORRESPONDANTES
Abstract
(EN) Peptides which bind a cellular (surface or intracellular) receptor, such as a nuclear receptor, may be identified by screening a combinatorial peptide library presented in the form of cells each of which coexpress one member peptide and the receptor, together with a signal producing system for reporting binding. A 'two-hybrid' assay is of particular interest. The screen may be carried out in the presence of a ligand, in particular, an exogenous ligand. If this screening is carried out for a plurality of different receptor conformations, then this library screening will also serve to identify conformation-specific peptides for the receptor, which may then be used in a panel for 'fingerprinting'query compounds as to their ability to interact with the receptor in the presence of each of the panel peptides. These fingerprints may be compared to those of reference compounds with known biological activities mediated by that receptor.
(FR) L'invention concerne un procédé d'identification de peptides qui se lient avec un récepteur cellulaire (en surface ou intracellulaire), du type récepteur nucléaire, par criblage de bibliothèque de peptides combinatoire se présentant sous la forme de cellules qui co-expriment chacune un peptide de la bibliothèque et le récepteur, avec utilisation de système de production de signaux notifiant la liaison. A cet égard, le dosage en 'deux hybrides' présente un intérêt particulier. Le criblage peut être réalisé en présence d'un ligand, en particulier un ligand exogène. S'il s'applique à une pluralité de conformations différentes de récepteurs, ce criblage de bibliothèque permet aussi d'identifier les peptides spécifiques à telle ou telle conformation pour le récepteur considéré, offrant ainsi la possibilité d'une utilisation dans un panel de peptides pour relever les 'empreintes digitales' peptidiques de composés d'intérêt dont on cherche à déterminer le potentiel d'interaction avec le récepteur en présence de chacun des peptides du panel. Il est possible de comparer les empreintes en question avec celles de composés de référence pour lesquels la médiation d'activités biologiques connues est assurée par le récepteur concerné.
Latest bibliographic data on file with the International Bureau