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1. WO2002004658 - COMPOSITIONS TO MEASURE PROTEIN CONCENTRATION

Publication Number WO/2002/004658
Publication Date 17.01.2002
International Application No. PCT/US2000/022357
International Filing Date 16.08.2000
IPC
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/6839
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6827Total protein determination, e.g. albumin in urine
6839involving dyes, e.g. Coomassie blue, bromcresol green
Applicants
  • CYTOSKELETON, INC. [US]/[US]
Inventors
  • DAVIS, Ashley
  • MIDDLETON, Kim
Common Representative
  • DAVIS, Ashley
Priority Data
09/610,95006.07.2000US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) COMPOSITIONS TO MEASURE PROTEIN CONCENTRATION
(FR) COMPOSITIONS PERMETTANT DE MESURER UNE CONCENTRATION DE PROTEINES
Abstract
(EN) Protein assays have general utility in the fields of research, clinical, diagnostic and other biotechnology areas. This invention relates to a novel protein assay reagent that has improved characteristics such as speed, simplicity, visible semi-quantitation with a color coded chart and low variance between proteins. The process of making and storing the reagent is also described. Figure (1) illustrates optimizing the dye content of the invention by varying the Serva blue G dye content between 35 and 70 mg/l, and keeping BSA at 100 ug protein per ml of reagent. Absorbance was measured at 595 nm.
(FR) L'invention concerne des analyses protéiques généralement utilisées dans le domaine de la recherche, le domaine clinique, le domaine diagnostique et d'autres domaines de la biotechnologie. L'invention concerne un nouveau réactif d'analyse protéique présentant des caractéristiques améliorées telles que la vitesse, la simplicité, la semi-quantification visible par un graphique chromocodé et une faible variance entre les protéines. L'invention concerne également un procédé de fabrication et de stockage du réactif. La figure 1 illustre l'optimisation du contenu en colorant de l'invention grâce à la variation du contenu en colorant Serva Blue G entre 35 et 70mg/l, et au maintien de l'albumine bovine (BSA) à 100ug de protéine par ml de réactif. L'absorption est mesurée à 595nm.
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