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1. WO2002000842 - METHODS FOR ISOLATING CENTROMERE DNA

Publication Number WO/2002/000842
Publication Date 03.01.2002
International Application No. PCT/US2001/019966
International Filing Date 22.06.2001
Chapter 2 Demand Filed 23.01.2002
IPC
C07H 21/00 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12N 15/1034
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
C12N 15/82
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
C12Q 1/6837
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
6837using probe arrays or probe chips
Applicants
  • THE UNIVERSITY OF CHICAGO [US]/[US] (AllExceptUS)
  • PREUSS, Daphne [US]/[US] (UsOnly)
  • COPENHAVER, Gregory [US]/[US] (UsOnly)
  • KEITH, Kevin [US]/[US] (UsOnly)
  • LUO, Song [CN]/[US] (UsOnly)
Inventors
  • PREUSS, Daphne
  • COPENHAVER, Gregory
  • KEITH, Kevin
  • LUO, Song
Agents
  • HANSON, Robert, E.
  • DEHMEL, Albrecht
Priority Data
60/228,79323.06.2000US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR ISOLATING CENTROMERE DNA
(FR) METHODES D'ISOLATION D'ADN CENTROMERE
Abstract
(EN) The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods represents an advance over the prior art in that costly and labor intensive mapping programs are not required. Using the technique, methylated centromere DNA may be isolated from potentially any centromere in an organism. The technique is amenable to mass screenings employing use of arrays comprising libraries of DNA from a target species.
(FR) L'invention concerne des méthodes efficaces d'isolation de centromères à partir d'un organisme. Ces méthodes constituent des avancées par rapport aux antériorités en ce qu'elles ne nécessitent pas de programmes de cartographie coûteux et à prédominance de main-d'oeuvre. L'utilisation de cette technique permet d'isoler de l'ADN centromère méthylé à partir d'un centromère quelconque de l'organisme. Cette technique s'applique au dépistage systématique à l'aide de réseaux de banques d'ADN d'une espèce cible.
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