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1. WO2002000169 - PRODUCTION OF VACCINES USING TRANSGENIC PLANTS

Publication Number WO/2002/000169
Publication Date 03.01.2002
International Application No. PCT/US2001/020272
International Filing Date 26.06.2001
Chapter 2 Demand Filed 24.01.2002
IPC
C07K 14/08 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
C07K 14/125 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
115Paramyxoviridae, e.g. parainfluenza virus
125Newcastle disease virus
C07K 14/16 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
155Lentiviridae, e.g. human immunodeficiency virus (HIV), visna-maedi virus, equine infectious anaemia virus
16HIV-1
C12N 15/82 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C07K 14/005
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C12N 15/8257
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8257for the production of primary gene products, e.g. pharmaceutical products, interferon
C12N 15/8258
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8242with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
8257for the production of primary gene products, e.g. pharmaceutical products, interferon
8258for the production of oral vaccines (antigens) or immunoglobulins
C12N 2740/16122
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2740Reverse Transcribing RNA Viruses
00011Reverse Transcribing RNA Viruses
10011Retroviridae
16011Human Immunodeficiency Virus, HIV
16111concerning HIV env
16122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
C12N 2760/18122
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2760ssRNA Viruses negative-sense
00011ssRNA Viruses negative-sense
18011Paramyxoviridae
18111Avulavirus, e.g. Newcastle disease virus
18122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Applicants
  • THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF AGRICULTURE [US]/[US]
Inventors
  • HAMMOND, Rosemarie
  • ZHAO, Yan
  • HAMMOND, John
Agents
  • PENDORF, Stephan, A.
Priority Data
09/603,99726.06.2000US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) PRODUCTION OF VACCINES USING TRANSGENIC PLANTS
(FR) PRODUCTION DE VACCINS PAR UTILISATION DE PLANTES TRANSGENIQUES OU DE VIRUS DE PLANTES MODIFIES COMME VECTEURS D'EXPRESSION ET PROTEINES DE COQUE VIRALES TRANSENCAPSIDEES UTILISEES COMME SYSTEMES DE PRESENTATION D'EPITOPES
Abstract
(EN) Plants infected with potato virus Y (PVY) were inoculated with infectious PVX RNA encoding PVX coat protein (CP) and modified chimeric NDV/BYMV CP. Antigen-coated plate indirect ELISA and immunoelectron microscopy of virus purified from infected plants showed that progeny virions contained from <1% to as much as 25% chimeric CP. The method can be used to produce and purify large amounts of NDV vaccine. Transencapsidated PVY virions expressing NDV were purified and used to induce anti-NDV antibodies in mice. Further, transgenic tobacco plants expressing HIV/BYMV CP were inoculated with BYMV. Progeny virions from transgenic plants contained transgenic HIV/BYMV CP.
(FR) Selon l'invention, des plantes infectées pas le virus Y de la pomme de terre (PVY) ont été inoculées avec de l'ARN de PVX infectieux codant pour la protéine de coque (PC) de PVX et pour la protéine PC chimérique de NDV/BYMV. L'ELISA indirect d'une plaque recouverte d'antigène et l'immunoélectromicroscopie d'un virus purifié à partir de plantes infectées ont montré que les virions descendants présentaient une concentration de protéine PC chimérique comprise entre moins de 1 % et 25 %. Une méthode peut être utilisée pour produire et purifier le vaccin contre NDV en grande quantité. Des virions de PVY transencapsidés exprimant NDV ont été purifiés et utilisés pour induire des anticorps dirigés contre NDV dans des souris. Par ailleurs, des plantes de tabac transgéniques exprimant la protéine PC de VIH/BYMV ont été inoculées avec BYMV. Les virions descendants issus de plantes transgéniques contenaient la protéine PC transgénique de VIH/BYMV.
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