Some content of this application is unavailable at the moment.
If this situation persists, please contact us atFeedback&Contact
1. (WO2001072751) PYRROLOPYRIMIDINES AS TYROSINE KINASE INHIBITORS
Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

PYRROLOPYRIMIDINES AS TYROSINE KINASE INHIBITORS

BACKGROUND OF THE INVENTION
There are at least 400 enzymes identified as protein kinases. These enzymes catalyze the phosphorylation of target protein substrates. The phosphorylation is usually a transfer reaction of a phosphate group from ATP to the protein substrate. The specific structure in the target substrate to which the phosphate is transferred is a tyrosine, serine or threonine residue. Since these amino acid residues are the target structures for the phosphoryl transfer, these protein kinase enzymes are commonly referred to as tyrosine kinases or serine/threonine kinases.
The phosphorylation reactions, and counteracting phosphatase reactions, at the tyrosine, serine and threonine residues are involved in countless cellular processes that underlie responses to diverse intracellular signals (typically mediated through cellular receptors), regulation of cellular functions, and activation or deactivation of cellular processes. A cascade of protein kinases often participate in intracellular signal transduction and are necessary for the realization of these cellular processes. Because of their ubiquity in these processes, the protem kinases can be found as an integral part of the plasma membrane or as cytoplasmic enzymes or localized in the nucleus, often as components of enzyme complexes. In many instances, these protein kinases are an essential element of enzyme and structural protein complexes that determine where and when a cellular process occurs within a cell.
Protein Tyrosine Kinases. Protein tyrosine kinases (PTKs) are enzymes which catalyse the phosphorylation of specific tyrosine residues in cellular proteins. This post-translational modification of these substrate proteins, often enzymes themselves, acts as a molecular switch regulating cell proliferation, activation or differentiation (for review, see Schlessinger and Ullrich, 1992, Neuron 9:383-391). Aberrant or excessive PTK activity has been observed in many disease states including benign and malignant proliferative disorders as well as diseases resulting from inappropriate activation of the immune system (e.g., autoimmune disorders), allograft rejection, and graft vs. host disease. In addition, endothelial-cell specific receptor PTKs such as KDR and Tie-2 mediate the angiogenic process, and are thus involved in supporting the progression of cancers and other diseases involving inappropriate vascularization (e.g., diabetic retinopathy, choroidal
neovascularization due to age-related macular degeneration, psoriasis, arthritis, retinopathy of prematurity, infantile hemangiomas).
Tyrosine kinases can be of the receptor-type (having extracellular, transmembrane and intracellular domains) or the non-receptor type (being wholly intracellular).
Receptor Tyrosine Kinases (RTKs). The RTKs comprise a large family of transmembrane receptors with diverse biological activities. At present, at least nineteen (19) distinct RTK subfamilies have been identified. The receptor tyrosine kinase (RTK) family includes receptors that are crucial for the growth and differentiation of a variety of cell types (Yarden and Ullrich, Ann. Rev. Biochem. 57:433-478, 1988; Ullrich and Schlessinger, Cell 61 :243-254, 1990). The intrinsic function of RTKs is activated upon ligand binding, which results in phosphorylation of the receptor and multiple cellular substrates, and subsequently in a variety of cellular responses (Ullrich & Schlessinger, 1990, Cell 61:203-212). Thus, receptor tyrosine kinase mediated signal transduction is initiated by extracellular interaction with a specific growth factor (ligand), typically followed by receptor dimerization, stimulation of the intrinsic protein tyrosine kinase activity and receptor trans-phosphorylation. Binding sites are thereby created for intracellular signal transduction molecules and lead to the formation of complexes with a spectrum of cytoplasmic signaling molecules that facilitate the appropriate cellular response. (e.g., cell division, differentiation, metabolic effects, changes in the extracellular microenvironment) see Schlessinger and Ullrich, 1992, Neuron 9:1-20.
Proteins with SH2 (src homology -2) or phosphotyrosine binding (PTB) domains bind activated tyrosine kinase receptors and their substrates with high affinity to propagate signals into cell. Both of the domains recognize
phosphotyrosine. (Fantl et al, 1992, Cell 69:413-423; Songyang et al, 1994, Mol.

Cell Biol. 14:2777-2785; Songyang et al, 1993, Cell 12:161-11S; and Koch et al, 1991, Science 252:668-678; Shoelson, Curr. Opin. Chem. Biol (1997), 1(2), 227-234; Cowburn, Curr. Opin. Struct. Biol (1997), 7(6), 835-838). Several
intracellular substrate proteins that associate with receptor tyrosine kinases (RTKs) have been identified. They may be divided into two principal groups: (1) substrates which have a catalytic domain; and (2) substrates which lack such a domain but serve as adapters and associate with catalytically active molecules (Songyang et al, 1993, Cell 72:767-778). The specificity of the interactions between receptors or proteins and SH2 or PTB domains of their substrates is determined by the amino acid residues immediately surrounding the phosphorylated tyrosine residue. For example, differences in the binding affinities between SH2 domains and the amino acid sequences surrounding the phosphotyrosine residues on particular receptors correlate with the observed differences in their substrate phosphorylation profiles (Songyang et al, 1993, Cell 72:767-778). Observations suggest that the function of each receptor tyrosine kinase is determined not only by its pattern of expression and ligand availability but also by the array of downstream signal transduction pathways that are activated by a particular receptor as well as the timing and duration of those stimuli. Thus, phosphorylation provides an important regulatory step which determines the selectivity of signaling pathways recruited by specific growth factor receptors, as well as differentiation factor receptors.
Several receptor tyrosine kinases such as FGFR-1, PDGFR, TIE-2 and c-Met, and growth factors that bind thereto, have been suggested to play a role in angiogenesis, although some may promote angiogenesis indirectly (Mustonen and Alitalo, J. Cell Biol. 129:895-898, 1995). One such receptor tyrosine kinase, known as "fetal liver kinase 1" (FLK-1), is a member of the type III subclass of RTKs. An alternative designation for human FLK-1 is "kinase insert domain-containing receptor" (KDR) (Terman et al, Oncogene 6:1677-83, 1991). Another alternative designation for FLK-1/KDR is "vascular endothelial cell growth factor receptor 2" (NEGFR-2) since it binds VEGF with high affinity. The murine version of FLK-l/VEGFR-2 has also been called ΝYK (Oelrichs et al, Oncogene 8(1):11-15, 1993).

DNAs encoding mouse, rat and human FLK-1 have been isolated, and the nucleotide and encoded amino acid sequences reported (Matthews et al, Proc. Natl. Acad. Sci. USA, 88:9026-30, 1991; Terman et al, 1991, supra; Terman et al, Biochem. Biophys. Res. Comm. 187:1579-86, 1992; Sarzani et al, supra; and Millauer et al, Cell 72:835-846, 1993). Numerous studies such as those reported in

Millauer et al, supra, suggest that VEGF and FLK-1 /KDR/VEGFR-2 are a ligand-receptor pair that play an important role in the proliferation of vascular endothelial cells, and formation and sprouting of blood vessels, termed vasculogenesis and angiogenesis, respectively.
Another type III subclass RTK designated "fins-like tyrosine kinase- 1" (Flt- 1) is related to FLK-1/KDR (DeVries et al. Science 255;989-991, 1992; Shibuya et al., Oncogene 5:519-524, 1990). An alternative designation for Flt-1 is "vascular endothelial cell growth factor receptor 1" (VEGFR-1). To date, members of the FLK-1/ KDR/VEGFR-2 and Flt-1/ VEGFR-1 subfamilies have been found expressed primarily on endothelial cells. These subclass members are specifically stimulated by members of the vascular endothelial cell growth factor (VEGF) family of ligands (Klagsburn and D'Amore, Cytokine & Growth Factor Reviews 7: 259-270, 1996). Vascular endothelial cell growth factor (VEGF) binds to Flt-1 with higher affinity than to FLK-1 /KDR and is mitogenic toward vascular endothelial cells (Terman et al., 1992, supra; Mustonen et al. supra; DeVries et al., supra). Flt- 1 is believed to be essential for endothelial organization during vascular
development. Flt-1 expression is associated with early vascular development in mouse embryos, and with neovascularization during wound healing (Mustonen and Alitalo, supra). Expression of Flt-1 in monocytes, osteoclasts, and osteoblasts, as well as in adult tissues such as kidney glomeruli suggests an additional function for this receptor that is not related to cell growth (Mustonen and Alitalo, supra).
As previously stated, recent evidence suggests that VEGF plays a role in the stimulation of both normal and pathological angiogenesis (Jakeman et al,
Endocrinology 133: 848-859, 1993; Kolch et al, Breast Cancer Research and Treatment 36: 139-155, 1995; Ferrara et al, Endocrine Reviews 18(1); 4-25, 1997;

Ferrara et al., Regulation of Angiogenesis (ed. L. D. Goldberg and E.M. Rosen), 209-232, 1997). In addition, VEGF has been implicated in the control and enhancement of vascular permeability (Connolly, et al, J. Biol. Chem. 264: 20017-20024, 1989; Brown et al, Regulation of Angiogenesis (ed. L.D. Goldberg and E.M. Rosen), 233-269, 1997). Different forms of VEGF arising from alternative splicing of mRNA have been reported, including the four species described by Ferrara et al. (J. Cell. Biochem. 47:211-218, 1991). Both secreted and predominantly cell-associated species of VEGF have been identified by Ferrara et al supra, and the protein is known to exist in the form of disulfide linked dimers.
Several related homologs of VEGF have recently been identified. However, their roles in normal physiological and disease processes have not yet been elucidated. In addition, the members of the VEGF family are often coexpressed with VEGF in a number of tissues and are, in general, capable of forming heterodimers with VEGF. This property likely alters the receptor specificity and biological effects of the heterodimers and further complicates the elucidation of their specific functions as illustrated below (Korpelainen and Alitalo, Curr. Opin. Cell Biol, 159-164, 1998 and references cited therein).
Placenta growth factor (PIGF) has an amino acid sequence that exhibits significant homology to the VEGF sequence (Park et al, J. Biol. Chem. 269:25646-54, 1994; Maglione et al. Oncogene 8:925-31, 1993). As with VEGF, different species of PIGF arise from alternative splicing of mRNA, and the protein exists in dimeric form (Park et al, supra). P1GF-1 and P1GF-2 bind to Flt-1 with high affinity, and P1GF-2 also avidly binds to neuropilin-1 (Migdal et al, J. Biol. Chem. 273 (35): 22272-22278), but neither binds to FLK-1/KDR (Park et al, supra). PIGF has been reported to potentiate both the vascular permeability and mitogenic effect of VEGF on endothelial cells when VEGF is present at low concentrations
(purportedly due to heterodimer formation) (Park et al, supra).
VEGF-B is produced as two isofoπns (167 and 185 residues) that also appear to bind Fit- 1/VEGFR- 1. It may play a role in the regulation of extracellular matrix degradation, cell adhesion, and migration through modulation of the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1 (Pepper et al, Proc. Natl. Acad. Sci. U. S. A. (1998), 95(20): 11709-11714).
VEGF-C was originally cloned as a ligand for VEGFR-3/Flt-4 which is primarily expressed by lymphatic endothelial cells. In its fully processed form, VEGF-C can also bind KDR/VEGFR-2 and stimulate proliferation and migration of endothelial cells in vitro and angiogenesis in in vivo models ( Lymboussaki et al, Am. J. Pathol (1998), 153(2): 395-403; Witzenbichler et al, Am. J. Pathol (1998), 153(2), 381-394). The transgenic overexpression of VEGF-C causes proliferation and enlargement of only lymphatic vessels, while blood vessels are unaffected. Unlike VEGF, the expression of VEGF-C is not induced by hypoxia (Ristimaki et al, J. Biol. Chem. (1998), 273(14),8413-8418).
The most recently discovered VEGF-D is structurally very similar to VEGF-C. VEGF-D is reported to bind and activate at least two VEGFRs, VEGFR-3/Flt-4 and KDR/VEGFR-2. It was originally cloned as a c-fos inducible mitogen for fibroblasts and is most prominently expressed in the mesenchymal cells of the lung and skin (Achen et al, Proc. Natl. Acad. Sci. U. S. A. (1998), 95(2), 548-553 and references therein).
As for VEGF, VEGF-C and VEGF-D have been claimed to induce increases in vascular permeability in vivo in a Miles assay when injected into cutaneous tissue (PCT/US97/14696; WO98/07832, Witzenbichler et al, supra). The physiological role and significance of these ligands in modulating vascular hyperpermeability and endothelial responses in tissues where they are expressed remains uncertain.
There has been recently reported a virally encoded, novel type of vascular endothelial growth factor, VEGF-E (NZ-7 VEGF), which preferentially utilizes KDR/Flk-l receptor and carries a potent mitotic activity without heparin-binding domain (Meyer et al, EMBO J. (1999), 18(2), 363-374; Ogawa et al, J. Biol Chem. (1998), 273(47), 31273-31282.). VEGF-E sequences possess 25% homology to mammalian VEGF and are encoded by the parapoxvirus Orf virus (OV). This parapoxvirus that affects sheep and goats and occasionally, humans, to generate lesions with angiogenesis. VEGF-E is a dimer of about 20 kDa with no basic domain nor affinity for heparin, but has the characteristic cysteine knot motif present in all mammalian VEGFs, and was surprisingly found to possess potency and bioactivities similar to the heparin-binding VEGF165 isoform of VEGF-A, i.e. both factors stimulate the release of tissue factor (TF), the proliferation, chemotaxis and sprouting of cultured vascular endothelial cells in vitro and angiogenesis in vivo.

Like VEGF 165, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation and a biphasic rise in free intracellular Ca2+ concentrations, while in contrast to VEGF 165, VEGF-E did not bind to VEGF receptor-1 (Flt-1).
Based upon emerging discoveries of other homologs of VEGF and VEGFRs and the precedents for ligand and receptor heterodimerization, the actions of such VEGF homologs may involve formation of VEGF ligand heterodimers, and/or heterodimerization of receptors, or binding to a yet undiscovered VEGFR
(Witzenbichler et al, supra). Also, recent reports suggest neuropilin-1 (Migdal et al, supra) or VEGFR-3/Flt-4 (Witzenbichler et al, supra), or receptors other than

KDR/NEGFR-2 may be involved in the induction of vascular permeability (Stacker, S.A., Vitali, A., Domagala, T., Nice, E., and Wilks, A.F., "Angiogenesis and Cancer" Conference, Amer. Assoc. Cancer Res., Jan. 1998, Orlando, FL; Williams, Diabetelogia 40: S118-120 (1997)).
Tie-2 (TEK) is a member of a recently discovered family of endothelial cell specific receptor tyrosine kinases which is involved in critical angiogenic processes, such as vessel branching, sprouting, remodeling, maturation and stability. Tie-2 is the first mammalian receptor tyrosine kinase for which both agonist ligand(s) (e.g., Angiopoietinl ("Angl"), which stimulates receptor autophosphorylation and signal transduction), and antagonist ligand(s) (e.g., Angiopoietin2 ("Ang2")), have been identified. Knock-out and transgenic manipulation of the expression of Tie-2 and its ligands indicates tight spatial and temporal control of Tie-2 signaling is essential for the proper development of new vasculature. The current model suggests that stimulation of Tie-2 kinase by the Angl ligand is directly involved in the branching, sprouting and outgrowth of new vessels, and recruitment and interaction of periendothelial support cells important in maintaimng vessel integrity and inducing quiescence. The absence of Angl stimulation of Tie-2 or the inhibition of Tie-2 autophosphorylation by Ang2, which is produced at high levels at sites of vascular regression, may cause a loss in vascular structure and matrix contacts resulting in endothelial cell death, especially in the absence of growth/survival stimuli. The situation is however more complex, since at least two additional Tie-2 ligands (Ang3 and Ang4) have recently been reported, and the capacity for heterooligomerization of the various agonistic and antagonistic angiopoietins, thereby modifying their activity, has been demonstrated. Targeting Tie-2 ligand-receptor interactions as an antiangiogenic therapeutic approach is thus less favored and a kinase inhibitory strategy preferred.
The soluble extracellular domain of Tie-2 ("ExTek") can act to disrupt the establishment of tumor vasculature in a breast tumor xenograft and lung metastasis models and in tumor-cell mediated ocular neovasculatization. By adenoviral infection, the in vivo production of mg/ml levels ExTek in rodents may be achieved for 7-10 days with no adverse side effects. These results suggest that disruption of Tie-2 signaling pathways in normal healthy animals may be well tolerated. These Tie-2 inhibitory responses to ExTek may be a consequence sequestration of ligand(s) and/or generation of a nonproductive heterodimer with full-length Tie-2.
Recently, significant upregulation of Tie-2 expression has been found within the vascular synovial pannus of arthritic joints of humans, consistent with a role in the inappropriate neovascularization. This finding suggests that Tie-2 plays a role in the progression of rheumatoid arthritis. Point mutations producing constitutively activated forms of Tie-2 have been identified in association with human venous malformation disorders. Tie-2 inhibitors are, thereful, useful in treating such disorders, and in other situations of inappropriate neovascularization.
The Non-Receptor Tyrosine Kinases. The non-receptor tyrosine kinases represent a collection of cellular enzymes which lack extracellular and
transmembrane sequences. At present, over twenty-four individual non-receptor tyrosine kinases, comprising eleven (11) subfamilies (Src, Frk, Btk, Csk, Abl, Zap70, Fes/Fps, Fak, Jak, Ack and LIMK) have been identified. At present, the Src subfamily of non-receptor tyrosine kinases is comprised of the largest number of PTKs and include Src, Yes, Fyn, Lyn, Lck, Blk, Hck, Fgr and Yrk. The Src subfamily of enzymes has been linked to oncogenesis and immune responses. A more detailed discussion of non-receptor tyrosine kinases is provided in Bohlen,

1993, Oncogene 8:2025-2031, which is incorporated herein by reference.
Many of the tyrosine kinases, whether an RTK or non-receptor tyrosine kinase, have been found to be involved in cellular signaling pathways involved in numerous pathogenic conditions, including cancer, psoriasis, and other
hyperproliferative disorders or hyper-immune responses.
Development of Compounds to Modulate the PTKs. In view of the surmised importance of PTKs to the control, regulation, and modulation of cell proliferation, the diseases and disorders associated with abnormal cell proliferation, many attempts have been made to identify receptor and non-receptor tyrosine kinase "inhibitors" using a variety of approaches, including the use of mutant ligands (U.S.

Application No. 4,966,849), soluble receptors and antibodies (Application No. WO 94/10202; Kendall & Thomas, 1994, Proc. Natl. Acad. Sci 90:10705-09; Kim et al, 1993, Nature 362:841-844), RNA ligands (Jellinek, etal, Biochemistry 33:10450-56; Takano, et al, 1993, Mol. Bio. Cell 4:358A; Kinsella, et al. 1992, Exp. Cell Res. 199:56-62; Wright, et al, 1992, J. Cellular Phys. 152:448-57) and tyrosine kinase inhibitors (WO 94/03427; WO 92/21660; WO 91/15495; WO 94/14808; U.S. Patent No. 5,330,992; Mariani, et al, 1994, Proc. Am. Assoc. Cancer Res. 35:2268).
More recently, attempts have been made to identify small molecules which act as tyrosine kinase inhibitors. For example, bis monocyclic, bicyclic or heterocyclic aryl compounds (PCT WO 92/20642) and vinylene-azaindole derivatives (PCT WO 94/14808) have been described generally as tyrosine kinase inhibitors. Styryl compounds (U.S. Patent No. 5,217,999), styryl-substituted pyridyl compounds (U.S. Patent No. 5,302,606), certain quinazoline derivatives (EP
Application No. 0 566 266 Al; Expert Opin. Ther. Pat. (1998), 8(4): 475-478), selenoindoles and selenides (PCT WO 94/03427), tricyclic polyhydroxylic compounds (PCT WO 92/21660) and benzylphosphonic acid compounds (PCT WO 91/15495) have been described as compounds for use as tyrosine kinase inhibitors for use in the treatment of cancer. Anilinocinnolines (PCT WO97/34876) and quinazoline derivative compounds (PCT WO97/22596; PCT WO97/42187) have been described as inhibitors of angiogenesis and vascular permeability.
In addition, attempts have been made to identify small molecules which act as serine/threonine kinase inhibitors. For example, bis(indolylmaleimide) compounds have been described as inhibiting particular PKC serine/threonine kinase isoforms whose signal transducing function is associated with altered vascular permeability in VEGF-related diseases (PCT WO97/40830; PCT WO97/40831).

Plk-1 Kinase Inhibitors
Plk-1 is a serine/threonine kinase which is an important regulator of cell cycle progression. It plays critical roles in the assembly and the dynamic function of the mitotic spindle apparatus. Plk-1 and related kinases have also been shown to be closely involved in the activation and inactivation of other cell cycle regulators, such as cyclin-dependent kinases. High levels of Plk-1 expression are associated with cell proliferation activities. It is often found in malignant tumors of various origins. Inhibitors of Plk-1 are expected to block cancer cell proliferation by disrupting processes involving mitotic spindles and inappropriately activated cyclin-dependent kinases.

Cdc2/Cvclin B Kinase Inhibitors (Cdc2 is also known as cdkl)
Cdc2/cyclin B is another serine/threonine kinase enzyme which belongs to the cyclin-dependent kinase (cdks) family. These enzymes are involved in the critical transition between various phases of cell cycle progression. It is believed that uncontrolled cell proliferation, which is the hallmark of cancer is dependent upon elevated cdk activities in these cells. The inhibition of elevated cdk activities in cancer cells by cdc2/cyclin B kinase inhibitors could suppress proliferation and may restore the normal control of cell cycle progression.
The regulation of CDK activation is complex, but requires the association of the CDK with a member of the cyclin family of regulatory subunits (Draetta, Trends in Cell Biology, 3:287-289 (1993)); Murray and Kirschner, Nature, 339:275-280 (1989); Solomon et al, Molecular Biology of the Cell, 3:13-27 (1992)). A further level of regulation occurs through both activating and inactivating phosphorylations of the CDK subunit (Draetta, Trends in Cell Biology, 3:287-289 (1993)); Murray and Kirschner, Nature, 339:275-280 (1989); Solomon et al, Molecular Biology of the Cell, 3:13-27 (1992); Ducommun et al, EMBO Journal, 10:3311-3319 (1991); Gautier et al, Nature 339:626-629 (1989); Gould and Nurse, Nature, 342:39-45 (1989); Krek and Nigg, EMBO Journal, 10:3331-3341 (1991); Solomon et al, Cell, 63 : 1013 - 1024 (1990)). The coordinate activation and inactivation of different cyclin/CDK complexes is necessary for normal progression through the cell cycle (Pines, Trends in Biochemical Sciences, 18:195-197 (1993); Sherr, Cell, 73:1059-1065 (1993)). Both the critical Gl-S and G 2-M transitions are controlled by the activation of different cyclin/CDK activities. In GI, both cyclin D/CDK4 and cyclin

E/CDK2 are thought to mediate the onset of S-phase (Matsushima et al, Molecular & Cellular Biology, 14:2066-2076 (1994); Ohtsubo and Roberts, Science, 259:1908-1912 (1993); Quelle et al, Genes & Development, 7:1559-1571 (1993); Resnitzky et al, Molecular & Cellular Biology, 14:1669-1679 (1994)). Progression through S-phase requires the activity of cyclin A/CDK2 (Girard et al, Cell, 67:1169-1179 (1991); Pagano et al, EMBO Journal, 11:961-971 (1992); Rosenblatt etal, Proceedings of the National Academy of Science USA, 89:2824-2828 (1992);
Walker and Mailer, Nature, 354:314-317 (1991); Zindy et al, Biochemical & Biophysical Research Communications, 182:1144-1154 (1992)) whereas the activation of cyclin A cdc2 (CDK1) and cyclin B/cdc2 are required for the onset of metaphase (Draetta, Trends in Cell Biology, 3:287-289 (1993)); Murray and
Kirschner, Nature, 339:275-280 (1989); Solomon et al, Molecular Biology of the Cell, 3:13-27 (1992); Girard et al, Cell, 67:1169-1179 (1991); Pagano et al, EMBO Journal, 11:961-971 (1992); Rosenblatt et al, Proceedings of the National Academy of Science USA, 89:2824-2828 (1992); Walker and Mailer, Nature, 354:314-317

(1991); Zindy et al, Biochemical & Biophysical Research Communications, 182:1144-1154 (1992)). It is not surprising, therefore, that the loss of control of CDK regulation is a frequent event in hyperproliferative diseases and cancer.
(Pines, Current Opinion in Cell Biology, 4:144-148 (1992); Lees, Current Opinion in Cell Biology, 7:773-780 (1995); Hunter and Pines, Cell, 79:573-582 (1994)).
Inhibitors of kinases involved in mediating or maintaining disease states represent novel therapies for these disorders. Examples of such kinases include, but are not limited to: (1) inhibition of c-Src Brickell, Critical Reviews in Oncogenesis, 3:401-406 (1992); Courtneidge, Seminars in Cancer Biology, 5:236-246 (1994), raf (Powis, Pharmacology & Therapeutics, 62:57-95 (1994)) and the cyclin-dependent kinases (CDKs) 1, 2 and 4 in cancer (Pines, Current Opinion in Cell Biology, 4:144-148 (1992); Lees, Current Opinion in Cell Biology, 7:773-780 (1995); Hunter and Pines, Cell, 79:573-582 (1994)), (2) inhibition of CDK2 or PDGF-R kinase in restenosis (Buchdunger et al, Proceedings of the National Academy of Science USA, 92:2258-2262 (1995)), (3) inhibition of CDK5 and GSK3 kinases in Alzheimers

(Hosoi et al, Journal of Biochemistry (Tokyo), 117:741-749 (1995); Aplin et al, Journal ofNeurochemistry, 67:699-707 (1996), (4) inhibition of c-Src kinase in osteoporosis (Tanaka et al, Nature, 383:528-531 (1996), (5) inhibition of GSK-3 kinase in type-2 diabetes (Borthwick et al, Biochemical & Biophysical Research Communications, 210:738-745 (1995), (6) inhibition of the p38 kinase in
inflammation (Badger et al, The Journal of Pharmacology and Experimental Therapeutics, 279:1453-1461 (1996)), (7) inhibition of VEGF-R 1-3 and TIE-1 and -2 kinases in diseases which involve angiogenesis (Shawver et al, Drug Discovery Today, 2:50-63 (1997)), (8) inhibition of UL97 kinase in viral infections (He et al, Journal of Virology, 71:405-411 (1997)), (9) inhibition of CSF- IR kinase in bone and hematopoetic diseases (Myers et al, Bioorganic & Medicinal Chemistry Letters, 7:421-424 (1997), and (10) inhibition of Lck kinase in autoimmune diseases and transplant rejection (Myers et al, Bioorganic & Medicinal Chemistry Letters, 7:417-420 (1997)).
It is additionally possible that inhibitors of certain kinases may have utility in the treatment of diseases when the kinase is not misregulated, but it nonetheless essential for maintenance of the disease state. In this case, inhibition of the kinase activity would act either as a cure or palliative for these diseases. For example, many viruses, such as human papilloma virus, disrupt the cell cycle and drive cells into the S-phase of the cell cycle (Vousden, FASEB Journal, 7:8720879 (1993)). Preventing cells from entering DNA synthesis after viral infection by inhibition of essential S-phase initiating activities such as CDK2, may disrupt the virus life cycle by preventing virus replication. This same principle may be used to protect normal cells of the body from toxicity of cycle-specific chemotherapeutic agents (Stone et al, Cancer Research, 56:3199-3202 (1996); Kohn et al, Journal of Cellular Biochemistry, 54:44-452 (1994)). Inhibition of CDKs 2 or 4 will prevent progression into the cycle in normal cells and limit the toxicity of cytotoxics which act in S-phase, G2 or mitosis. Furthermore, CDK2/cyclin E activity has also been shown to regulate NF-kB. Inhibition of CDK2 activity stimulates NF-kB-dependent gene expression, an event mediated through interactions with the p300 coactivator (Perkins et al, Science, 275:523-527 (1997)). NF-kB regulates genes involved in inflammatory responses (such as hematopoetic growth factors, chemokines and leukocyte adhesion molecules) (Baeuerle and Henkel, Annual Review of
Immunology, 12:141-179 (1994)) and may be involved in the suppression of apoptotic signals within the cell (Beg and Baltimore, Science, 274:782-784 (1996); Wang et al, Science, 274:784-787 (1996); Van Antwerp et al, Science, 274:787-789 (1996)). Thus, inhibition of CDK2 may suppress apoptosis induced by cytotoxic drugs via a mechanism which involves NF-kB. This therefore suggests that inhibition of CDK2 activity may also have utility in other cases where regulation of NF-kB plays a role in etiology of disease. A further example may be take from fungal infections: Aspergillosis is a common infection in immune-compromised patients (Armstrong, Clinical Infectious Diseases, 16:1-7 (1993)). Inhibition of the Aspergillus kinases Cdc2/CDC28 or Nim A (Osmani et al, EMBO Journal, 10:2669-2679 (1991); Osmani et al, Cell, 67:283-291 (1991)) may cause arrest or death in the fungi, improving the therapeutic outcome for patients with these infections.
The identification of effective small compounds which specifically inhibit signal transduction and cellular proliferation by modulating the activity of receptor and non-receptor tyrosine and serine/threonine kinases to regulate and modulate abnormal or inappropriate cell proliferation, differentiation, or metabolism is therefore desirable. In particular, the identification of methods and compounds that specifically inhibit the function of a tyrosine kinase which is essential for angiogenic processes or the formation of vascular hyperpermeability leading to edema, ascites, effusions, exudates, and macromolecular extravasation and matrix deposition as well as associated disorders would be beneficial.

SUMMARY OF THE INVENTION

The present invention provides compounds of Formula I,



(I)

and pharmaceutically acceptable salts thereof.
In Formula I, Ring A is a six membered aromatic ring or a five or six ' membered heteroaromatic ring. Ring A is optionally substituted such as with one or more of the following substituents: a substituted or unsubstituted aliphatic group, a halogen, a substituted or unsubstituted aromatic group, substituted or unsubstituted heteroaromatic group, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aralkyl, substituted or unsubstituted heteroaralkyl, cyano, nitro, -NR4R5, -C(O)2H, -OH, a substituted or unsubstituted alkoxycarbonyl, -C(O)2-haloalkyl, a substituted or unsubstituted alkylthio ether, a substituted or unsubstituted alkylsulfoxide, a substituted or unsubstituted alkylsulfone, a substituted or unsubstituted arylthio ether, a substituted or unsubstituted arylsulfoxide, a substituted or unsubstituted arylsulfone, a substituted or unsubstituted alkyl carbonyl, -C(O)-haloalkyl, a substituted or unsubstituted aliphatic ether, a substituted or unsubstituted aromatic ether, carboxamido, tetrazolyl, trifluoromethylsulphonamido,
trifluoromethylcarbonylamino, a substituted or unsubstituted alkynyl, a substituted or unsubstituted alkyl amido, a substituted or unsubstituted aryl a ido, a substituted or unsubstituted styryl and a substituted or unsubstituted aralkyl amido.
L is one of the following linkers: -O-; -S-; -S(O)-; -S(O)2-; -N(R)-; -N(C(O)OR)-; -N(C(O)R)-; -N(SO2R)-; -CH2O-; -CH2S-; -CH2N(R)-; -CH(NR)-;-CH2N(C(O)R))-; -CH2N(C(O)OR)-;-CH2N(SO2R)-; -CH(NHR)-; -CH(NHC(0)R)-; -CH(NHSO2R)-; -CH(NHC(O)OR)-;-CH(OC(O)R)-;-CH(OC(O)NHR)-; -CH=CH-; -C(=NOR)-; -C(O)-; -CH(OR)-;-C(O)N(R)-; -N(R)C(O)-; -N(R)S(O)-;-N(R)S(O)2-;-OC(O)N(R)-;-N(R)C(O)N(R)-; -NRC(O)O-;-S(O)N(R)-;-S(O)2N(R)-;
N(C(O)R)S(O)-; N(C(O)R)S(O)2-; -N(R)S(O)N(R)-; -N(R)S(O)2N(R)-; -C(O)N(R)C(O)-; -S(O)N(R)C(O)-; -S(O)2N(R)C(O)-; -OS(O)N(R)-; -OS(O)2N(R)-; -N(R)S(O)O-; -N(R)S(O)2O-; -N(R)S(O)C(O)-; -N(R)S(O)2C(O)-; -SON(C(O)R)-; -SO2N(C(O)R)-; -N(R)SON(R)-; -N(R)SO2N(R)-; -C(O)O-; -N(R)P(OR')O-; -N(R)P(OR')-; -N(R)P(O)(OR')O-; -N(R)P(O)(OR ; -N(C(O)R)P(OR')O-; -N(C(O)R)P(OR')-; -N(C(O)R)P(O)(OR')O-, -N(C(O)R)P(OR')-, CH(R)S(O)-; -CH(R)S(O)2-; -CH(R)N(C(O)OR)-; -CH(R)N(C(O)R)-; -CH(R)N(SO2R); -CH(R)O-; -CH(R)S-; -CH(R)N(R)-; -CH(R)N(C(O)R))-; -CH(R)N(C(O)OR)-;-CH(R)N(SO2R)-; - CH(R)C(=NOR)-; -CH(R)C(O)-; -CH(R)CH(OR)-;-CH(R)C(O)N(R)-; - CH(R)N(R)C(O)-; -CH(R)N(R)S(O)-;-CH(R)N(R)S(O)2-;-CH(R)OC(O)N(R ; -CH(R)N(R)C(O)N(R)-; -CH(R)NRC(O)O-;- CH(R)S(0)N(R)-;-CH(R)S(O)2N(R)-; -CH(R)N(C(O)R)S(O)-; - CH(R)N(C(O)R)S(0)2-; -CH(R)N(R)S(O)N(R>; -CH(R)N(R)S(O)2N(R)-; -CH(R)C(O)N(R)C(O)-; -CH(R)S(O)N(R)C(O)-; -CH(R)S(O)2N(R)C(O)-; - CH(R)OS(O)N(R)-; -CH(R)OS(O)2N(R)-; -CH(R)N(R)S(O)O-; - CH(R)N(R)S(O)2O-; -CH(R)N(R)S(O)C(O)-; -CH(R)N(R)S(O)2C(O)-; - CH(R)SON(C(0)R)-; -CH(R)SO2N(C(O)R)-; -CH(R)N(R)SON(R)-; - CH(R)N(R)SO2N(R)-; -CH(R)C(O)O-; -CH(R)N(R)P(OR')O-; - CH(R)N(R)P(OR')-; -CH(R)N(R)P(O)(OR')O-; -CH(R)N(R)P(O)(OR')-; - CH(R)N(C(0)R)P(OR')O-; -CH(R)N(C(O)R)P(OR')-; - CH(R)N(C(O)R)P(O)(OR')O- or -CH(R)N(C(O)R)P(OR')-. R and R' are each, independently, -H, an acyl group, a substituted or unsubstituted aliphatic group, a substituted or unsubstituted aromatic group, a substituted or unsubstituted heteroaromatic group, or a substituted or unsubstituted cycloalkyl group.
Alternatively, L is -RbN(R)S(O)2-, -RbN(R)P(O)-, or -RbN(R)P(O)O-. R„ is an alkylene group which when taken together with the sulphonamide,
phosphinamide, or phosphonamide group to which it is bound forms a five or six membered ring fused to ring A.
Alternatively, L is represented by one of the following structural formulas:




R85 taken together with the phosphinamide, or phosphonamide is a 5-, 6-, or

7-membered, aromatic, heteroaromatic or heterocycloalkyl ring system.
In Formula I, R, is a substituted aliphatic group, a substituted cycloalkyl, a substituted bicycloalkyl, a substituted cycloalkenyl, an optionally substituted aromatic group, an optionally substituted heteroaromatic group, an optionally substituted heteroaralkyl, an optionally substituted heterocycloalkyl, an optionally substituted heterobicycloalkyl, an optionally substituted alkylamindo, and optionally substituted arylamido, an optionally substituted -S(O)2-alkyl or optionally substituted -S(O)2-cycloalkyl, a -C(O)-alkyl or an optionally substituted -C(O)-alkyl.
Rj can be substituted with one or more substituents. Preferably, Rj is substituted with a substituted or unsubstituted aliphatic group, a substituted or unsubstituted aromatic group, a substituted or unsubstituted heteroaromatic, a substituted or unsubstituted aralkyl, a substituted or unsubstituted heteroaralkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted
heterocycloalkyl, a substituted or unsubstituted aromatic ether, a substituted or unsubstituted aliphatic ether, a substituted or unsubstituted alkoxycarbonyl, a substituted or unsubstituted alkylcarbonyl, a substituted or unsubstituted
arylcarbonyl, a substituted or unsubstituted heteroarylcarbonyl, substituted or unsubstituted aryloxycarbonyl, -OH, a substituted or unsubstituted aminocarbonyl, an oxime, a substituted or unsubstituted azabicycloalkyl, heterocycloalkyl, oxo, aldehyde, a substituted or unsubstituted alkyl sulfonamido group, a substituted or unsubstituted aryl sulfonamido group, a substituted or unsubstituted bicycloalkyl, a substituted or unsubstituted heterobicycloalkyl, cyano, -NH2, an alkylamino, ureido, thioureido and -B-E.
B is a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted heterocycloalkyl, a substituted or unsubstituted aromatic, a substituted or unsubstituted heteroaromatic, an alkylene, an aminoalkyl, an alkylenecarbnonyl, or an aminoalkylcarbonyl.
E is a substituted or unsubstituted azacycloalkyl, a substituted or
unsubstituted azacycloalkylcarbonyl, a substituted or unsubstituted
azacycloalkylsulfonyl, a substituted or unsubstituted azacycloalkylalkyl, a substituted or unsubstituted heteroaryl, a substituted or unsubstituted
heteroarylcarbonyl, a substituted or unsubstituted heteroarylsulfonyl, a substituted or unsubstituted heteroaralkyl, a substituted or unsubstituted alkyl sulfonamido, a substituted or unsubstituted aryl sulfonamido, a substituted or unsubstituted bicycloalkyl, a substituted or unsubstituted ureido, a substituted or unsubstituted thioureido or a substituted or unsubstituted aryl.

G is a direct bond; -(CH2)j-, wherein j is 1 to 6; a C2-C6- alkenylene group, a C3-C8-cycloalkylene group or a CrC6-oxaalkylene group;
However, when Rj is an aliphatic group or cycloalkyl group, Rλ is not exclusively substituted with one or more substitutent selected from the group consisting of hydroxyl and lower alkyl ethers. In addition, a heterocycloalkyl is not 2-phenyl-l,3-dioxan-5-yl, and an aliphatic group is not substituted exclusively with one or more aliphatic groups.
In Formula I, R2 is -H, a substituted or unsubstituted aliphatic group, a substituted or unsubstituted cycloalkyl, a halogen, -OH, cyano, a substituted or unsubstituted aromatic group, a substituted or unsubstituted heteroaromatic group, a substituted or unsubstituted heterocycloalkyl, a substituted or unsubstituted aralkyl, a substituted or unsubstituted heteroaralkyl, -NR4R5, or -C(O)NR4R5.
In Formula I, R3 is a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aromatic group, a substituted or unsubstituted heteroaromatic group, or a substituted or unsubstituted heterocycloalkyl.
In Formula I, R4, R5 and the nitrogen atom together form a 3, 4, 5, 6 or 7-membered, substituted or unsubstituted heterocycloalkyl, substituted or
unsubstituted heterobicycloalkyl or a substituted or unsubstituted heteroaromatic.
Alternatively, R4 and R5 are each, independently, -H, azabicycloalkyl, heterocycloalkyl, a substituted or unsubstituted alkyl group or Y-Z.
Y is selected from the group consisting of -C(O)-, -(CH2)p-,-S(O)2-, -C(O)O-, -SO2NH-, -CONH-, (CH2)pO-, -(CH2)pNH-, -(CH2)pS-, -(CH2)pS(O)-, and -(CH2)pS(O)2-.
p is an integer from 0 to to about 6.
Z is a substituted or unsubstituted alkyl, substituted or unsubstituted amino, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl or substituted or unsubstituted heterocycloalkyl group.
j an integer from 0 to 6.
However, when L is -CH2NR-, -C(O)NR- or -NRC(O)- and R3 is azacycloalkyl or azaheteroaryl, j is 0. In addition, when L is -O- and R3 is phenyl, j is O.

In a preferred subset of the compounds of Formula I, Rj is B-E, where E is a heterocyclyl group; L is -CH2NHC(O)-; -CH2NHC(O)NH-; -CH2NHC(O)O-; -CH2C(O)NH-; -CH2NHS(O)2-; -NHC(O)-; -NHC(O)NH-; -NHC(O)O-; -C(0)NH--NS(O)2-; A is 1,4-phenylene or 1,4-phenylene substituted with one or more methoxy groups or fluorine atoms; R3 is phenyl or phenyl substituted with one or more substituents selected from the group consisting of chloro, cyano, bromo, fluoro, trifluoromethoxy, methoxy, methylenedioxy, methyl, amino, dimethylamino and nitro; R2 is hydrogen; and G is a direct bond or -(CH2)j-, wherein j is 0 to 4.
In another preferred subset of the compounds of Formula I, ring A is 1,4-phenylene, L is -O-, G is a direct bond and R3 is phenyl.
The compounds of this invention are useful as inhibitors of serine/threonine and tyrosine kinases. In particular, compounds of this invention are useful as inhibitors of tyrosine kinases that are important in hyperproliferative diseases, especially in cancer and in the process of angiogenesis. For example, certain of these compounds are inhibitors of such receptor kinases as KDR, Fit- 1 , FGFR, PDGFR, c-Met, TIE-2 or IGF-l-R. Since certain of these compounds are anti-angiogenic, they are important substances for inhibiting the progression of disease states where angiogenesis is an important component. Certain compounds of the invention are effective as inhbitors of such serine/threonine kinases as PKCs, erk, MAP kinases, MAP kinase kinases, MAP kinase kinase kinases, cdks, Plk-1 or Raf-1. These compounds are useful in the treatment of cancer, and hyperproliferative disorders. In addition, certain compounds are effective inhibitors of non-receptor kinases such as those of the Src (for example, Ick, blk and lyn), Tec, Csk, Jak, Map, Nik and Syk families. These compunds are useful in the treatment of cancer, hyperproliferative disorders and immunologic diseases.
Certain compounds of this invention are selective TIE-2 kinase inhibitors which may be anti-angiogenic (especially in combination with one or more VEGFR inhibitors), or pro-angiogenic, when employed in the presence of, or in conjunction with, a VEGF-related stimulus. In this manner such inhibitors can be used in the promotion of therapeutic angiogenesis to treat, for example, ischemia, infarct or occlusion, or to promote wound healing.

The present invention provides a method of inhibiting the kinase activity of tyrosine kinases and serine/threonine kinases comprising the administration of a compound represented by formula I to said kinase in sufficient concentration to inhibit the enzyme activity of said kinase.
The present invention further includes the use of these compounds in pharmaceutical compositions with a pharmaceutically effective amount of the above-described compounds and a pharmaceutically acceptable carrier or excipient. These pharmaceutical compositions can be administered to individuals to slow or halt the process of angiogenesis in angiogenesis-aided diseases, or to treat edema, effusions, exudates or ascites and other conditions associated with vascular hyperpermeability. Certain pharmaceutical compositions can be administered to individuals to treat cancer and hyperproliferative disorders by inhibiting serine/threonine kinases such as cdk, Plk-1, erk, etc.

DETAILED DESCRIPTION OF THE INVENTION
The values of substituents in a first preferred group of compounds of formula I are given below.
Preferably, L is -N(R)C(O)N(R)-, -N(R)S(O)2-, -S(O)2N(R)-, -N(R)C(O)-, -C(O)N(R)-, or-O-.
Preferably, G is a direct bond; -(CH2)r, wherein j is from 1 or 2; trans - CH=CH-; -cydoC3H4-; or-CH2O-.
Preferably, R3 is a substituted or unsubstituted phenyl, a substituted or unsubstituted naphthyl, a substituted or unsubstituted pyridyl, a substituted or unsubstituted thienyl, a substituted or unsubstituted benzotriazole, a substituted or unsubstituted tetrahydropyranyl, a substituted or unsubstituted tefrahydrofuranyl, a substituted or unsubstituted dioxane, a substituted or unsubstituted dioxolane, a substituted or unsubstituted quinoline, a substituted or unsubstituted thiazole, substituted or unsubstituted isoxazole, substituted or unsubstituted cyclopentanyl, a substituted or unsubstituted benzofuran, substituted or unsubstituted
benzothiophene, substituted or unsubstituted benzisoxazole, substituted or unsubstituted benzisothiazole, substituted or unsubstituted benzothiazole, substituted or unsubstituted benzoxazole, substituted or unsubstituted benzimidazole, substituted or unsubstituted benzoxadiazole, substituted or unsubstituted
benzothiadiazole, substituted or unsubstituted isoquinoline, substituted or unsubstituted quinoxaline, substituted or unsubstituted indole or substituted or unsubstituted pyrazole. Alternatively, R3 can be a substituted or unsubstituted aliphatic group or a substituted or unsubstituted alkenyl, provided that
L is -SN(R)-, -S(O)N(R)-, -S(O)2N(R , -N(R)S-, -N(R)S(O)-, -N(R)S(O)2-,
-N(R)C(O)N(R)-, -N(R)SN(R')-, -N(R)S(O)N(R')-, or -N(R)S(O)2N(R')-;
In one embodiment, R3 is a substituted or unsubstituted phenyl or phenyl fused to a five- or six-membered heterocyclic group. In this embodiment, suitable examples of R3 include, but are not limited to, the groups shown below.


where R is hydrogen or alkyl.
R3 can be substituted by one or more substituents. Preferable substituents for

R3 are F, Cl, Br, I, CH3, NO2, OCF3, OCH3, CN, CO2CH3, CF3, t-butyl, pyridyl, substituted or unsubstituted oxazolyl, substituted or unsubstituted benzyl, substituted or unsubstituted benzenesulfonyl, substituted or unsubstituted phenoxy, substituted or unsubstituted phenyl, substituted or unsubstituted amino, carboxyl, substituted or unsubstituted tetrazolyl, styryl, -S-(substituted or unsubstituted aryl), -S-(substituted or unsubstituted heteroaryl), substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, alkynyl, -C(O)NRfRg, R. , CH2OR,..
Rf, Rg and the nitrogen atom together form a 3-, 4-, 5-, 6- or 7-membered, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted heterobicycloalkyl or a substituted or unsubstituted heteroaromatic.

Alternatively, R_ and Rg are each, independently, a substituted or
unsubstituted aliphatic group or a substituted or unsubstuituted aromatic group.
Rc is hydrogen, or substituted or unsubstituted alkyl or substituted or unsubstituted aryl; -W-(CH2)t-NRdRe, -W-(CH2)t-O-alkyl, , -W-(CH2)t-S-alkyl, or -W-(CH2)rOH.
t is an integer from 0 to about 6.
W is a bond or -O-, -S-, -S(O)-, -S(O)2-, or -NRk-.
Rk is -H or alkyl.
Rd, Re and the nitrogen atom to which they are attached together form a 3, 4, 5, 6 or 7-membered substituted or unsubstituted heterocycloalkyl or substituted or unsubstituted heterobicyclic group.
Alternatively, Rd and Re are each, independently, -H, alkyl, alkanoyl or -K-D.
K is -S(O)2-, -C(O)-, -C(O)NH-, -C(O)2-, or a direct bond.
D is a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl, a substituted or unsubstituted aralkyl, a substituted or unsubstituted heteroaromatic group, a substituted or unsubstituted heteroaralkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted heterocycloalkyl, a substituted or unsubstituted amino, a substituted or unsubstituted aminoalkyl, a substituted or unsubstituted aminocycloalkyl, COORj, or substituted or unsubstituted alkyl.
R; is a substituted or unsubstituted aliphatic group or a substituted or unsubstituted aromatic group.
More preferred substituents for R3 are F, Cl, Br, I, cyano, nitro, OCF3, CH3, and CF3.
Preferably, ring A is a substituted or unsubstituted phenyl, a substituted or unsubstituted naphthyl, a substituted or unsubstituted pyridyl, or a substituted or unsubstituted indole. La one embodiment, ring A is a substituted or unsubstituted phenyl. In one embodiment, ring A is a substituted or unsubstituted 1,4-phenylene group which is optionally substituted with one or more methoxy or fluoro groups.

Ring A can be substituted by one or more substituents. Preferable substituents for ring A are F, Cl, Br, I, CH3, NO2, OCF3, OCH3, CN, CO2CH3, CF3, t-butyl, pyridyl, substituted or unsubstituted oxazolyl, substituted or unsubstituted benzyl, substituted or unsubstituted benzenesulfonyl, substituted or unsubstituted phenoxy, substituted or unsubstituted phenyl, substituted or unsubstituted amino, carboxyl, substituted or unsubstituted tetrazolyl, styryl, -S-(substituted or unsubstituted aryl), -S-(substituted or unsubstituted heteroaryl), substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, alkynyl, -C(O)NRfRg, R0 and CH2ORc. R_ , Rg and Rc are defined as above.
Ring A is more preferably substituted with F, Cl, and nitro.
R2 is preferably hydrogen.
In one embodiment, Rj is of the formula


m is an integer from 0 to about 3.
In another embodiment, Rt is of the formula



m, t are defined as above. R8, g and the nitrogen atom together form a 3-, 4-, 5-, 6-or 7-membered, substituted or unsubstituted heterocycloalkyl, a substituted or unsubstituted heteroaromatic or substituted or unsubstituted heterobicyclicalkyl group. Alternatively, R8 and Rg are each, independently, -H, azabicycloalkyl, heterocycloalkyl, alkyl; hydroxyalkyl; dihydroxyalkyl or Y2-Z2. Y2 is -C(O)-, -(CH2)q-,-S(O)2-, -C(O)O-, -SO2NH-, -CONH-, (CH2)qO-, -(CH2)qNH-, -(CH2)qS-, ■ (CH2)qS(O)-, or -(CH2)qS(O)2-. q is an integer from 0 to 6. Z2 is a substituted or unsubstituted alkyl, a substituted or unsubstituted amino, a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl or a substituted or unsubstituted heterocycloalkyl group.

In another embodiment, Rx is of the formula


m, t , R8, and R_ are defined as above, s is an integer from 0 to 6. q is an integer from 0 to about 6. R77 is -OR78, or -NR79R80. R78 is -H or a substituted or unsubstituted aliphatic group. R79, R80 and the nitrogen atom together form a 3, 4, 5, 6 or 7-membered, substituted or unsubstituted heterocycloalkyl group, substituted or unsubstituted heteroaryl group, or a substituted heterobicyclicalkyl group. R79 and R80 are each, independently, -H, azabicycloalkyl, heterocycloalkyl or — Y3-Z3. Y3 is selected from the group consisting of -C(O)-, -(CH2)q-,-S(O)2-, -C(0)O-, -SO2NH-, - CONH-, (CH2)qO-, -(CH2)qNH-, -(CH2)qS-, -(CH2)qS(O)- and-(CH2)qS(O)2-. Z3 is - H, a substituted or unsubstituted alkyl, a substituted or unsubstituted amino, a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl or a substituted or unsubstituted heterocycloalkyl. In one embodiment, m is 2; s is 0; and R77 is -OH. In one embodiment, Rj is selected from the groups shown below.





In another embodiment, R, is of the formula

1(d)

v is an integer from 1 to about 3. R10 is -H, azabicycloalkyl, heterocycloalkyl or Y2 Z2. Y2 and Z2 are as defined previously.
In another embodiment, Rj is of the formula



m and R10 are as previously defined. Rn represents one or more substituents independently selected from the group consisting of hydrogen, hydroxy, oxo, a substituted or unsubstituted aliphatic group, a substituted or unsubstituted aromatic group, a substituted or unsubstituted heteroaromatic group, a substituted or unsubstituted alkoxycarbonyl, a substituted or unsubstituted alkoxyalkyl, a substituted or unsubstituted aminocarbonyl, a substituted or unsubstituted alkylcarbonyl, a substituted or unsubstituted arylcarbonyl, a substituted or unsubstituted heteroarylcarbonyl, a substituted or unsubstituted aminoalkyl and a substituted or unsubstituted aralkyl groups, provided that the carbon atoms adjacent to the nitrogen atom are not substituted by a hydroxy group.

In another embodiment, Rt is of the formula


Ri0 is as previously defined. Preferably, R10 is methyl, isopropyl or methoxyethyl In another embodiment, Ri is of the formula


r is an integer from 1 to about 6. R8 and Rg are as previously defined.

Ih another embodiment, R1 is of the formula



R8, Rg and t are as previously defined, w is an integer from 0 to about 4. u is 0 or 1. R!2 is hydrogen or a substituted or unsubstituted alkyl group.
In another embodiment, Ri is of the formula


w, t, Rio, R12 are as previously defined.
In yet another embodiment, Rj is of the formula

wherein m is 0, 1 or 2; R8X and R82 are each, independently, selected from the group consisting of hydrogen, hydroxyl, cyanomethyl, carboxymethyl,
aminocarbonylmethyl, aminocarbonyl; aminomethyl, hydroxymethyl and amino,



provided that no more than one of R and R82 is hydrogen. R8j and RS2 can also together form oxo; -O-(CH2)rO, wherein i is 2 or 3; -NH-C(O)-NH-C(O)-; or-NH- C(O)-NH-CH2-. For example, Rt can be, but is not limited to, one of the groups shown below.

In another subset of the compounds of Formula I, R! is of the formula B-E, wherein B is cyclohexyl and E is substituted or unsubstituted heterocyclyl, heterocyclylalkylor heterocyclylazaalkyl. For example, E can be a substituted or unsubstituted pyrazolyl, diazepinyl, piperazyl, piperidyl or morpholyl group.
Suitable examples of R2 include, but are not limited to, the groups shown below.


In another embodiment, when Rt is 1(g) or 1(H), R8, Rg and the nitrogen atom together form a heterocycloalkyl group of the formula


u is as previously defined. R13, R14, R15, Rι6, R17, Ri8, R19 and R20 are each, independently, lower alkyl or hydrogen. Alternatively, at least one pair of substituents R13 and RM; R15 and Rl6; R17 and R18; or Rj9 and R20 together are an oxygen atom. Alternatively, at least one of R and Rl5 is cyano, CONHR21, COOR2ι, CH2OR2ι or CH2NR2ι(R22). R21, R22 and the nitrogen atom together form a 3-, 4-, 5-, 6- or 7-membered, substituted or unsubstituted heterocycloalkyl group, substituted or unsubstituted heteroaryl group, or a substituted heterobicyclicalkyl group. Alternatively, R2l and R22 are each, independently, -H, azabicycloalkyl, heterocycloalkyl or Y3-Z3; Y3 and Z3 are as previously defined. X is -O-, -S-, -SO-, -SO2~, -CH2-, -CH(OR23)- or NR23. R23 is -H, substituted or unsubstituted alkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted aralkyl, -C(NH)NH2, -C(O)R24, or -C(O)OR24. R24 is hydrogen, substituted or unsubstituted alkyl, a substituted or unsubstituted aryl or a substituted or unsubstituted aralkyl.
In another embodiment, R8, Rg and the nitrogen atom together form a heterocycloalkyl of the formula


t, R and R22 are as previously defined. R25 and R26 are each, independently, hydrogen or lower alkyl. Alternatively, R25 and R26 together are an oxygen atom, i is an integer from 1 to about 6.
In another embodiment, R8, Rg and the nitrogen atom together form a heterocycloalkyl group; of the formula


i is as previously defined. R27 is CH2OH, C(O)NR24R28 or COOR24. R24 and R28 are as previously defined.
In another embodiment, R8, Rg and the nitrogen atom together form a heteroaromatic group of the formula


R29 is a substituted or unsubstituted alkyl, a substituted or unsubstituted aryl or a substituted or unsubstituted aralkyl group, carboxylic acid, cyano, C(O)OR30,

CH2OR30, CH2NR2ιR22 or C(O)NR21R22. R30 is a substituted or unsubstituted alkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted aralkyl, a substituted or unsubstituted heterocycloalkyl or heterocycloaryl group. R and R22 are as previously defined.
In another embodiment, at least one of R8 and Rg is of the formula Y3-D, wherein D is of the formula


Y3 is as previously defined, x is 0, 1 or 2. T is -O-, -C(O)-, -S-, -SO-, -SO2-, -CH2-, -CH(OR24)- or -N(R24)-. R24 is as previously defined.
In another embodiment, at least one of R8 and Rg is of the formula Y3- N(R31)R32, Y3 is as previously defined. R3i and R32 are each, independently, substituted or unsubstituted carboxyalkyl, a substituted or unsubstituted
alkoxycarbonylalkyl, a substituted or unsubstituted hydroxyalkyl, a substituted or unsubstituted alkylsulfonyl, a substituted or unsubstituted alkylcarbonyl or a substituted or unsubstituted cyanoalkyl. Alternatively, R3X and R32, together with the nitrogen atom, form a five- or six-membered heterocycloalkyl group, a substituted or unsubstituted heteroaromatic or a substitutituted or unsubstituted heterobicycloalkyl.

In another embodiment, when R{ is 1(e), Z2 is of the formula N(R35)R36. R35 and R36 are each, independently, hydrogen, alkyl, alkoxycarbonyl, alkoxyalkyl, hydroxyalkyl, aminocarbonyl, cyano, alkylcarbonyl or aralkyl.
In another embodiment, when Ri is 1(e), Z2 is of the formula


Each Xj is, independently, CH or N. R37 is hydrogen, cyano or a substituted or unsubstituted alkyl, a substituted or unsubstituted alkoxycarbonyl, a substituted or unsubstituted alkoxyalkyl, a substituted or unsubstituted hydroxyalkyl, a substituted or unsubstituted aminocarbonyl, a substituted or unsubstituted alkylcarbonyl or a substituted or unsubstituted aralkyl group.

In another embodiment, when Rλ is 1(e), Z2 is of the formula


g is an integer from 0 to about 3. T is as previously defined. R37 is hydrogen, cyano or a substituted or unsubstituted alkyl, a substituted or unsubstituted alkoxycarbonyl, a substituted or unsubstituted alkoxyalkyl, a substituted or unsubstituted
hydroxyalkyl, a substituted or unsubstituted aminocarbonyl, a substituted or unsubstituted alkylcarbonyl or a substituted or unsubstituted aralkyl group.
In another embodiment, when Rt is 1(e), Z2 is of the formula


g and R37 are as previously defined unsubstituted aralkyl group.
In another embodiment, when Ri is 1(e), Z2 is of the formula

T, g and R37 are as previously defined.
In another embodiment, when Ri is 1(e), Z2 is of the formula


R37 is as previously defined. R38 is hydrogen, substituted or unsubstituted alkyl, a ' substituted or unsubstituted alkoxycarbonyl, a substituted or unsubstituted alkoxyalkyl, a substituted or unsubstituted aminocarbonyl, perhaloalkyl, a substituted or unsubstituted alkenyl, a substituted or unsubstituted alkylcarbonyl or a substituted or unsubstituted aralkyl.
In another embodiment, Ri is of the formula



u is as previously defined. R39, R40, R4i, R42, R43, , R45 and R46 are each, independently ,methyl or hydrogen. Alternatively, at least one pair of substituents R39 and R40; R36 and R37; R38 and R39. Alternatively, R40 and R together are an oxygen atom. R47 is H, azabicycloalkyl, heterocycloalkyl or Y2-Z2. Y2 and Z2 are as previously defined. Alternatively, R47 is of the formula


y is 0 or 1. R48, R49, R50, R5ι, R52, R53, R5 and R55 are each, independently, methyl or hydrogen. Alternatively, at least one pair of substituents R48 and R49; R50 and R5ι; R52 and R53; or R54 and R55 together are an oxygen atom. R_6 is -H, azabicycloalkyl, heterocycloalkyl or Y3-Z3. Y3 and Z3 are defined as above.
In another embodiment, Rt is of the formula


e, f, h, u and y are independently 0 or 1. R57, R58, Rsg, R60, R«, Rβ2, es, R.4>.R65 and R66 are each, independently, methyl or hydrogen. Alternatively, at least one pair of substituents R57 and R^; R59 and R^; R6i and Rg2; or R63 and R_4 together are an oxygen atom. R67 is H, azabicycloalkyl, heterocycloalkyl or Y2-Z2. Y2 and Z2 are defined as above. Alternatively, R67 is of the formula

d is 0 or 1. R68, Rg9, R70, R71, R72, R73, R74 and R75 are each, independently, lower alkyl or hydrogen. Alternatively, at least one pair of substituents R68 and R69; R70 and R71; R72 and R73. R74 and R75 together are an oxygen atom. R76 is -H, azabicycloalkyl, heterocycloalkyl or Y3-Z3. Y3 and Z3 are defined as above.
As used herein, aromatic groups include carbocyclic ring systems (e.g.
benzyl and cinnamyl) and fused polycyclic aromatic ring systems (e.g. naphthyl and 1,2,3,4-tetrahydronaphthyl). Aromatic groups are also referred to as aryl groups herein.
Heteroaromatic groups, as used herein, include heteroaryl ring systems (e.g., thienyl, pyridyl, isoxazolyl, thiadiazolyl, oxadiazolyl, indazolyl, furanyls, pyrroles, ' imidazoles, pyrazoles, triazoles, pyrimidines, pyrazyls, thiazolyls, isoxazolyls, isothiazolyls, tetrazolyls, oxadiazolyls,) and heteroaryl ring systems in which a carbocyclic aromatic ring, carbocyclic non-aromatic ring or heteroaryl ring is fused to one or more other heteroaryl rings (e.g., benzo(b)thienyl, benzimidazole, indole, tetrahydroindole, azaindole, indazole, quinoline, imidazopyridine, purine, pyrrolo[2,3-d]pyrimidine, pyrazolo[3,4-d]pyrimidine, 2,1,3-benzoxadiazolyl, 2,1,3- benzothiadiazolyl, benzoxazolyl, 3,4-dihydro-2H-benzoxazyl, benzothiazolyl, quinazolyl, quinoxalyl, isoquinolyl, indolizyl) and their N-oxides.
An aralkyl group, as used herein, is an aromatic substituent that is linked to a compound by an aliphatic group having from one to about six carbon atoms.
An heteroaralkyl group, as used herein, is a heteroaromatic substituent that is linked to a compound by an aliphatic group having from one to about six carbon atoms.
A heterocycloalkyl group, as used herein, is a non-aromatic ring system that has 3 to 8 atoms and includes at least one heteroatom, such as nitrogen, oxygen, or sulfur.
An acyl group, as used herein, is an -C(O)NRxRz, -C(O)ORx, -C(O)R-., in which R_ and R_ are each, independently, -H, a substituted or unsubstituted aliphatic group or a substituted or unsubstituted aromatic group.
As used herein, aliphatic groups include straight chained, branched or cyclic -Cg hydrocarbons which are completely saturated or which contain one or more units of unsaturation (e.g. one or more double or triple bonds). The term "alkyl" refers to a saturated hydrocarbyl group; "alkoxy" refers to an alkyl-O- group. A "lower alkyl group" is a saturated aliphatic group having form 1-6 carbon atoms; a "lower alkoxy group" is a lower-alkyl-O- group.
As used herein, the term "oxaalkylene" refers to an alkylene chain which is interrupted at one or more points by an oxygen atom. Examples of oxaalkylene groups include, but are not limited to, -OCH2-, -CH2O- and -CH2OCH2-.
For substituted groups described above, substituents can include, but are not to be construed as being limited to, one or more substituents independently selected from halo, hydroxy, oxo, nitro, amino, mono- or di-alkylamino, alkoxy, cyano, perfluoroalkyl (preferably CF3), perfluoroalkoxy (preferably OCF3), COOR (where R is H or alkyl), carboxamide, acetyl, cycloalkyl, aryloxy, heteroaryl, heteroaryloxy, heterocycloalkyl, amido, aminocarbonyl, alkylthio ether, alkylsulfonyl,
alkylsulfonamido, aliphatic group (optionally substituted with one or more of the following: halo, hydroxy, oxo, nitro, amino, mono- or di-alkylamino, alkoxy, cyano, perfluoroalkyl, perfluoroalkoxy and COOR (where R is H or alkyl)), phenyl (optionally substituted with one or more of the following: halo, hydroxy, nitro, amino, alkylamino, mono- or di-alkylamino, mono- or di-alkylaminoalkyl, alkoxy, cyano, perfluoroalkyl, perfluoroalkoxy and COOR (where R is H or alkyl)).
Compounds of formula I may exist as salts with pharmaceutically acceptable acids. The present invention includes such salts. Examples of such salts include hydrochlorides, hydrobromides, sulfates, methanesulfonates, nitrates, maleates, acetates, citrates, fumarates, tartrates [eg (+)-tartrates, (-)-tartrates or mixtures thereof including racemic mixtures], succinates, benzoates and salts with amino acids such as glutamic acid. These salts may be prepared by methods known to those skilled in the art.
Certain compounds of formula I which have acidic substituents may exist as salts with pharmaceutically acceptable bases. The present invention includes such salts. Example of such salts include sodium salts, potassium salts, lysine salts and arginine salts. These salts may be prepared by methods known to those skilled in the art.
Certain compounds of formula I and their salts may exist in more than one crystal form and the present invention includes each crystal form and mixtures thereof.
Certain compounds of formula I and their salts may also exist in the form of solvates, for example hydrates, and the present invention includes each solvate and mixtures thereof.
Certain compounds of formula I may contain one or more chiral centres, and exist in different optically active forms. When compounds of formula I contain one chiral centre, the compounds exist in two enantiomeric forms and the present invention includes both enantiomers and mixtures of enantiomers, such as racemic mixtures. The enantiomers may be resolved by methods known to those skilled in the art, for example by formation of diastereoisomeric salts which may be separated, for example, by crystallization; formation of diastereoisomeric derivatives or complexes which may be separated, for example, by crystallization, gas-liquid or liquid chromatography; selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic esterification; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support for example silica with a bound chiral ligand or in the presence of a chiral solvent. It will be appreciated that where the desired enantiomer is converted into another chemical entity by one of the separation procedures described above, a further step is required to liberate the desired enantiomeric form. Alternatively, specific enantiomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer into the other by asymmetric transformation.

When a compound of formula I contains more than one chiral center it may exist in diastereoisomeric forms. The diastereoisomeric pairs may be separated by methods known to those skilled in the art, for example chromatography or crystallization and the individual enantiomers within each pair may be separated as described above. The present invention includes each diastereoisomer of
compounds of formula I and mixtures thereof.
Certain compounds of formula I may exist in different tautomeric forms or as different geometric isomers, and the present invention includes each tautomer and/or geometric isomer of compounds of formula I and mixtures thereof.
Certain compounds of formula I may exist in different stable conformational forms which may be separable. Torsional asymmetry due to restricted rotation about an asymmetric single bond, for example because of steric hindrance or ring strain, may permit separation of different conformers. The present invention includes each conformational isomer of compounds of formula I and mixtures thereof.
Certain compounds of formula I may exist in zwitterionic form and the present invention includes each zwitterionic form of compounds of formula I and mixtures thereof.
A preferred group of compounds of the present invention are:

Cw-5-(4-phenoxyphenyl)-7-(4-pyrrolidinocyclohex-l-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine

rrαn_.-5-(4-phenoxyphenyl)-7-(4-pyrrolidinocyclohex-l-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine

Cw-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohex-l-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine hydrochloride

Era?._.-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohex-l-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine Eraπ_.-7-(4-dimethylaιninocyclohexyl)-5-(4-phenoxyphenyl)-7H-pyrrolo
[2,3-d]pyrimidin-4-ylamine

Ct5-7-(4-dimemylaminocyclohexyl)-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine

5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pyrrolo[2,3-_ ]pyrimidin-4-ylamine dihydrochloride
5-(4-phenoxyphenyl)-7-(3-pyrrolidinyl) -7H-pyrrolo[2,3-cT]pyrimidin-4-ylamine dihydrochloride

Cώ-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-

Jrα«5-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-J]pyrimidin-4-amine

C _f-7-{4-[4-(2-methoxyethyl)piperazino]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-_ ]pyrimidin-4-amine

7 _.«_.-7-{4-[4-(2-methoxyethyl)piperazino]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-_ ]pyrimidin-4-amine

Ct_?-7-[-4-(4-ethylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3- ]pyrimidin-4-amine

trαn_f-7-[4-(4-ethylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-_ ]pyrimidin-4-amine

Ct_.-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3- c?]pyrimidin-4-amine tris maleate

_ r .5-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3- ]pyrimidin-4-amine tris maleate

Ct5-7-{4-[4-(2-methoxyethyl)piperazino]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-cT|pyrimidin-4-amine tris maleate

_Tran_.-7-{4-[4-(2-methoxyethyl)piperazino]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pyιτolo[2,3- ^pyrimidin-4-amine tris maleate

Cis-l-(4- { [3 -( IH- 1 -imidazolyl)propyl] amino } cyclohexyl)-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-(_]pyrimidin-4-amine trimaleate salt

Trans-l-(4- {[3-(lH-l -imidazolyl)propyl] amino } cy clohexyl)-5 -(4-phenoxyphenyl)-7H-pyrrolo[2,3-_ ]pyrimidin-4-amine dimaleate salt

Cz_.-7-[4-(dimethylamino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-<i]pyrimidin-4-amine dimaleate salt

rr_.«5-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohexyl)-7H-pyrrolo[2,3-J]pyrimidin-4-amine dimaleate salt

_ rarø-5-(4-phenoxyphenyl)-7-(4-tetrahydro-lH-l-pyrrolylcyclohexyl)-7H-pyrrolo[2,3-J]pyrimidin-4-amine dimaleate salt

Ct5-5-(4-phenoxyphenyl)-7-(4-piperazinocyclohexyl)-7H-pyrrolo[2,3-.f]pyr_midin-4-amine trimaleate salt

rrαR5-5-(4-phenoxyphenyl)-7-(4-piperazinocyclohexyl)-7H-pyrrolo[2,3-_i]pyrimidin-4-amine trimaleate salt 7-[3-(4-methylpiperazino)cyclopentyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-cT]pyrimidin-4-amine tri-maleate

_ rαw-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-_ ]pyrimidin-4-amine

rrΩR_.-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3- ]ρyrimidin-4-amine tri-maleate

trαn-.-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3- ]pyrimidin-4-amine tri-hydrochloride

cis-1 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-cTjpyrimidin-4-amine tri-maleate salt

cis-1 -[3 -(4-methylpiperazino)cyclohexyl]-5 -(4-phenoxyphenyl)-7H-pyrrolo [2,3 -JJpyrimidin-4-amine tri-hydrochloride

Er n5-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo [2 ,3 -_φyrimidin-4-amine trimaleate

Cis- benzyl N-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7Η-pyrrolo[2,3-d]pyrimidin-5-yl} 2-methoxyphenyl)carbamate tri-maleate

Trans- benzyl N-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyi]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-methoxyphenyl)carbamate tri-maleate

Er «_.-Nl-(4-{4-aιnino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5 -yl} -2-methoxyphenyl)benzamide rran5-Nl-(4-{4-an_dno-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-djpyrimidin-5-yl} -2-methoxyphenyl)benzamide tri-maleate

Ct5-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-methoxyphenyl)-3-phenylpropanamide

rrα«_- Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-methoxyphenyl)-3-phenylpropanamide

cis- Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-^pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide trimaleate salt

trα7._.-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-cT]pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide tri-maleate

ct_f-2-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-ylphenoxy)-6-[(3-methoxypropyl)amino]benzonitrile tri-maleate

trαn5-2-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-<i]pyrimidin-5-ylphenoxy)-6-[(3-methoxypropyl)amino]benzonitrile tri-maleate

ct-.-2-amino-6-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-ύf]pyrimidin-5-ylphenoxy)benzonitrile tri-maleate

trαR_.-2-ammo-6-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-J]pyrimidin-5-ylphenoxy)benzorιitrile tri-maleate

ct_.-2-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-_ ]pyrimidin-5-ylphenoxy)-6-[(4-methylphenyl)sulfanyl]benzonitrile tri-maleate

trαn_.-2-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3- <f[pyrimidin-5-ylphenoxy)-6-[(4-methylphenyl)sulfanyl]benzonitrile tri-maleate

ct_f-2-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-_i]pyriιrήdm-5-ylphenoxy)-6-(2-pyridylsulfanyl)benzonitrile tri-maleate

tr_zn5-2-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-rf]pyrimidin-5-ylphenoxy)-6-(2-pyridylsulfanyl)benzonitrile tri-maleate

ct5-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-_f|pyrimidin-4-amine tri-maleate

trα?2_.-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-_ ]pyrimidin-4-amine tri-maleate

cis-Nl -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7Η-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l-benzenesulfonamide tri-maleate

trans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-4-fluoro- 1 -benzenesulfonamide tri-maleate

Nl-4-[4-ammo-7-(l-benzyl-4-piperidyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro- 1 -benzenesulfonamide

Nl-4-[4-amino-7-(l-benzyl-4-piperidyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-2,3-dichloro-l-benzenesulfonamide

Nl-4-[4-amino-7-(4-piperidyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro- 1 -benzenesulfonamide Nl-4-[4-amino-7-(l-formyl-4-piperidyl)-7H-pyιτolo[2,3-d]pvrimidin-5-yl]-2-fluoroρhenyl-4-fluoro- 1 -benzenesulfonamide

Nl-[4-(4-amino-7-l-[(l-methyl-lH-4-imidazolyl)sulfonyl]-4-piperidyl-7H-pyrrolo[2,3-d]pyrimidm-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide dimaleate

Nl-[4-(4-amino-7-l-[(l,2-dimethyl-lH-4-imidazolyl)sulfonyl]-4-piperidyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide

Nl-[4-(4-amino-7-l-[(l,3-dimethyl-lH-5-pyrazolyl)carbonyl]-4-piperidyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide

Nl-(4-{4-amino-7-[l-(2-pyridylcarbonyl)-4-piperidyl]-7H-pyιτolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-4-fluoro- 1 -benzenesulfonamide

Nl-4-(4-amino-7-{4-[l-(l-methylpiperid-4-yl)piperidyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl})-2-fluorophenyl-4-fluoro-l-benzenesulfonamide tri-maleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-(trifluoromethoxy)- 1 -benzenesulfonamide trimaleate

trans-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-5-chloro-2-thiophenesulfonamide
benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3- djpyrimidin-5-yl} -2-fluorophenyl)-2-chloro-4-fluoro- 1 -benzenesulfonamide
benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3-dichloro-l-benzenesulfonamide trimaleate

cis-N- 1 -(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyrrolo [2,3 -djpyrimidin-5-yl} -2-fluorophenyl)-2-chloro-4-fluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrin idin-5-yl-2-fluorophenyl)-2,5-difluoro-l-benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,6-difluoro-l-benzenesulfonamide trimaleate

trans- N-4-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2, 1 ,3-benzothiadiazole-4-sulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3,4-trifluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-me1hylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2 -nitro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyιτolo[2,3-d]pyrirnidin-5-yl} -2-fluorophenyl)-2-fluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyιτolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,4,6-trichloro- 1 -benzenesulfonamide trimaleate cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,6-dichloro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-chloro-l -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)- 3 -fluoro- 1 -benzenesulfonamide dimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-5-chloro-2-thiophenesulfonamide dimaleate

cis-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-4-bromo-2,6-difluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyiimidin-5-yl}-2-fluorophenyl)-3-chloro-4-fluoro-l-benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl-2-iodo-l-benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-(trifluoromethoxy)- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3-dichloro-l-berιzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin- 5-yl}-2-fluorophenyl)-2-chloro-6-methyl-l-benzenesulfonamide trimaleate

cis-N-l-(4-{4-anιino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-chloro-4-cyano- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-anιmo-7-[4-(4-me1hylpiperazmo)cyclohexyl]-7H-pyιτolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3,4-trifluoro-l-benzenesulfonamide trimaleate

cis-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-3 ,4-difluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-4-bromo-2-fluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-5-bromo-2-thiophenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,4-dichloro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3 ,4-trichloro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-3-bromo-5-chloro-2-thiophenesulfonamide trimaleate

cis- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2, 1 ,3-benzothiadiazole-4-sulfonamide trimaleate

cis- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3- d]pyrimidin-5-yl}-2-fluorophenyl)-2,l,3-benzoxadiazole-4-sulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,5-dichloro- 1 -thiophenesulfonamide trimaleate

cis- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(7-chloro-2, 1 ,3-benzoxadiazole)-4-sulfonamide trimaleate

cis- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(7-methyl-2, 1 ,3-benzothiadiazole)-4-sulfonamide trimaleate

cis- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(5-methyl-2, 1 ,3-benzothiadiazole)-4-sulfonamide trimaleate
cis- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(5-chloro-2, 1 ,3-benzothiadiazole)-4-sulfonamide trimaleate
cis-N- 1 -(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyrrolo [2,3 -djpyrimidin- 5-yl}-2-fluorophenyl)-3-chloro-2-methyl-l-benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-bromo-l-benzenesulfonamide trimaleate

cis-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,5-dibromo-3 ,6-difluoro- 1 -benzenesulfonamide trimaleate

cis-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3-dichloro- 1 -benzenesulfonamide frimaleate cis-N-l-(4-{4-an_ino-7-[4-(4-methylpiperazmo)cyclohexyl]-7H-pyιτolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)- (2-nitrophenyl)methanesulfonamide trimaleate

trans-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-nitro- 1 -benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-fluoro- 1 -benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,4,6-trichloro-l-benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,6-dichloro- 1 -benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2 -chloro- 1 -benzenesulfonamide trimaleate

trans-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)- 3-fluoro-l-benzenesulfonamide dimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidm-5-yl}-2-fluorophenyl)-4-bromo-2,5-difluoro-l-benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluoroρhenyl)-3-chloro-4-fluoro-l -benzenesulfonamide trimaleate trans-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3- d]pyrirm^m-5-yl}-2-fluorophenyl-2-iodo-l-benzenesulfonamide trimaleate

frans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3-dichloro- 1 -benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-chloro-6-methyl-l-benzenesulfonamide trimaleate

xrans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-chloro-4-cyano- 1 -benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-3,4-difluoro-l-benzenesulfonamide trimaleate

trans-N- 1 -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-4-bromo-2-fluoro- 1 -benzenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-5-bromo-2-thiophenesulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,4-dichloro-l-benzenesulfona_nide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3 ,4-trichloro- 1 -benzenesulfonamide trimaleate

frans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrinn^in-5-yl}-2-fluorophenyl)-3-bromo-5-chloro-2-tMophenesulfonamide frimaleate

frans- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3- d]pyrimidin-5-yl} -2-fluorophenyl)-2, 1 ,3-benzoxadiazole-4-sulfonamide trimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluoroρhenyl)-2,5-dichloro-l-thiophenesulfonamide trimaleate

trans- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-(7-chloro-2,l,3-benzoxadiazole)-4-sulfonamide trimaleate

trans- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(7-methyl-2, 1 ,3-benzotlιiadiazole)-4-sulfonamide trimaleate

trans- N-4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(5-methyl-2, 1 ,3-benzothiadiazole)-4-sulfonamide trimaleate
trans- -4-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-(5-chloro-2,l,3-benzotMadiazole)-4-sulfonamide trimaleate
trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-3-chloro-2-methyl-l-ber_zenesulfonamide frimaleate

trans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-bromo- 1 -benzenesulfonamide trimaleate

trans-N- 1 -(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyrrolo [2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,5-dibromo-3,6-difluoro-l-benzenesulfonamide trimaleate

frans-N-l-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3- d]pyrimidin-5-yl} -2-fluorophenyl)- (2-nitrophenyl)methanesulfonamide trimaleate

The compounds of this invention have antiangiogenic properties. These antiangiogenic properties are due at least in part to the inhibition of protein tyrosine kinases essential for angiogenic processes. For this reason, these compounds can be used as active agents against such disease states as arthritis, atherosclerosis, restenosis, psoriasis, hemangiomas, myocardial angiogenesis, coronary and cerebral collaterals, ischemic limb angiogenesis, ischemia reperfusion injury, wound healing, peptic ulcer Helicobacter related diseases, virally-induced angiogenic disorders, fractures, Crow-Fukase syndrome (POEMS), preeclampsia, menometrorrhagia, cat scratch fever, rubeosis, neovascular glaucoma and retinopathies such as those associated with diabetic retinopathy, retinopathy of prematurity, or age-related macular degeneration. In addition, some of these compounds can be used as active agents against solid tumors, malignant ascites, von Hippel Lindau disease, hematopoietic cancers and hyperproliferative disorders such as thyroid hyperplasia (especially Grave's disease), and cysts (such as hypervascularity of ovarian stroma characteristic of polycystic ovarian syndrome (Stein-Leventhal syndrome) and polycystic kidney disease since such diseases require a proliferation of blood vessel cells for growth and/or metastasis.
Further, some of these compounds can be used as active agents against burns, chronic lung disease, stroke, polyps, anaphylaxis, chronic and allergic inflammation, delayed-type hypersensitivity, ovarian hyperstimulation syndrome, brain tumor-associated cerebral edema, high-altitude, trauma or hypoxia induced cerebral or pulmonary edema, ocular and macular edema, ascites, glomerulonephritis and other diseases where vascular hyperpermeability, effusions, exudates, protein
extravasation, or edema is a manifestation of the disease. The compounds will also be useful in treating disorders in which protein extravasation leads to the deposition of fibrin and extracellular matrix, promoting stromal proliferation (e.g. keloid, fibrosis, cirrhosis and carpal tunnel syndrome). Increased VEGF production potentiates inflammatory processes such as monocyte recruitment and activation.

The compounds of this invention will also be useful in treating inflammatory disorders such as inflammatory bowel disease (IBD) and Crohn's disease.
VEGF's are unique in that they are the only angiogenic growth factors known to contribute to vascular hyperpermeability and the formation of edema. Indeed, vascular hyperpermeability and edema that is associated with the expression or administration of many other growth factors appears to be mediated via VEGF production. Inflammatory cytokines stimulate VEGF production. Hypoxia results in a marked upregulation of VEGF in numerous tissues, hence situations involving infarct, occlusion, ischemia, anemia, or circulatory impairment typically invoke VEGF/VPF mediated responses. Vascular hyperpermeability, associated edema, altered transendothelial exchange and macromolecular extravasation, which is often accompanied by diapedesis, can result in excessive matrix deposition, aberrant stromal proliferation, fibrosis, etc. Hence, VEGF-mediated hyperpermeability can significantly contribute to disorders with these etiologic features.
Because blastocyst implantation, placental development and embryogenesis are angiogenesis dependent, certain compounds of the invention areuseful as contraceptive agents and antifertility agents.
It is envisaged that the disorders listed above are mediated to a significant extent by protein tyrosine kinase activity involving the KDR VEGFR-2 and/or the Flt-l/VEGFR-1 and/or TIE-2 tyrosine kinases. By inhibiting the activity of these tyrosine kinases, the progression of the listed disorders is inhibited because the angiogenic or vascular hyperpermeability component of the disease state is severely curtailed. The action of certain compounds of this invention, by their selectivity for specific tyrosine kinases, result in a minimization of side effects that would occur if less selective tyrosine kinase inhibitors were used. Certain compounds of the invention are also effective inhibitors of FGFR, PDGFR, c-Met and IGF-l-R. These receptor kinases can directly or indirectly potentiate angiogenic and
hyperproliferative responses in various disorders, hence their inhibition can impede disease progression.
The compounds of this invention have inhibitory activity against protein kinases. That is, these compounds modulate signal transduction by protein kinases.

Compounds of this invention inhibit protein kinases from serine/threoriine and tyrosine kinase classes. In particular, these compounds selectively inhibit the activity of the KDR/FLK-1 /VEGFR-2 tyrosine kinases. Certain compounds of this invention also inhibit the activity of additional tyrosine kinases such as Flt-1/VEGFR-l, Tie-2, FGFR, PDGFR, IGF-IR, c-Met, Src-subfamily kinases such as Lck, Src, fyn, yes, etc. Additionally, some compounds of this invention
significantly inhibit serine/threonine kinases such as PKC, MAP kinases, erk, CDKs, Plk-1, or Raf-1 which play an essential role in cell proliferation and cell-cycle progression. The potency and specificity of the generic compounds of this invention towards a particular protein kinase can often be altered and optimized by variations in the nature, number and arrangement of the substituents (i.e., Rt, R2, R3, A and ring 1) and conformational restrictions. In addition the metabolites of certain compounds may also possess significant protein kinase inhibitory activity.
The compounds of this invention, when admimstered to individuals in need of such compounds, inhibit vascular hyperpermeability and the formation of edema in these individuals. These compounds act, it is believed, by inhibiting the activity of KDR tyrosine kinase which is involved in the process of vascular
hyperpermeability and edema formation. The KDR tyrosine kinase may also be referred to as FLK-1 tyrosine kinase, NYK tyrosine kinase or VEGFR-2 tyrosine kinase. KDR tyrosine kinase is activated when vascular endothelial cell growth factor (VEGF) or another activating ligand (such as VEGF-C, VEGF-D, VEGF-E or HIV Tat protein) binds to a KDR tyrosine kinase receptor which lies on the surface of vascular endothelial cells. Following such KDR tyrosine kinase activation, hyperpermeability of the blood vessels occurs and fluid moves from the blood stream past the blood vessel walls into the interstitial spaces, thereby forming an area of edema. Diapedesis also often accompanies this response. Similarly, excessive vascular hyperpermeability can disrupt normal molecular exchange across the endothelium in critical tissues and organs (e.g., lung and kidney), thereby causing macromolecular extravasation and deposition. Following this acute response to KDR stimulation which is believed to facilitate the subsequent angiogenic process, prolonged KDR tyrosine kinase stimulation results in the proliferation and chemotaxis of vascular endothelial cells and formation of new vessels. By inhibiting KDR tyrosine kinase activity, either by blocking the production of the activating ligand, by blocking the activating ligand binding to the KDR tyrosine kinase receptor, by preventing receptor dimerization and
transphosphorylation, by inhibiting the enzyme activity of the KDR tyrosine kinase (inhibiting the phosphorylation function of the enzyme) or by some other
mechanism that interrupts its downstream signaling (D. Mukhopedhyay et al, Cancer Res. 55.T278-1284 (1998) and references therein), hyperpermeability, as well as associated extravasation, subsequent edema formation and matrix deposition, and angiogenic responses, may be inhibited and minimized.
One group of prefened compounds of this invention have the property of inhibiting KDR tyrosine kinase activity without significantly inhibiting Flt-1 tyrosine kinase activity (Flt-1 tyrosine kinase is also referred to as VEGFR-1 tyrosine kinase). Both KDR tyrosine kinase and Flt-1 tyrosine kinase are activated by VEGF binding to KDR tyrosine kinase receptors and to Flt-1 tyrosine kinase receptors, respectively. Certain prefened compounds of this invention are unique because they inhibit the activity of one VEGF-receptor tyrosine kinase (KDR) that is activated by activating ligands but do not inhibit other receptor tyrosine kinases, such as Flt-1, that are also activated by certain activating ligands. In this manner, certain prefened compounds of this invention are, therefore, selective in their tyrosine kinase inhibitory activity.
In one embodiment, the present invention provides a method of treating a protein kinase-mediated condition in a patient, comprising adiminstering to the patient a therapeutically or prophylactically effective amount of one or more compounds of Formula I.
A "protein kinase-mediated condition" is a medical condition, such as a disease or other undesirable physical condition, the genesis or progression of which depends, at least in part, on the activity of at least one protein kinase. The protein kinase can be, for example, a protein tyrosine kinase or a protein serine/threonine kinase.
The patient to be treated can be any animal, and is preferably a mammal, such as a domesticated animal or a livestock animal. More preferably, the patient is a human.
A "therapeutically effective amount" is an amount of a compound of Formula I or a combination of two or more such compounds, which inhibits, totally or partially, the progression of the condition or alleviates, at least partially, one or more symptoms of the condition. A therapeutically effective amount can also be an amount which is prophylactically effective. The amount which is therapeutically effective will depend upon the patient's size and gender, the condition to be treated, the severity of the condition and the result sought. For a given patient, a
therapeutically effective amount can be determined by methods known to those of skill in the art.
The method of the present invention is useful in the treatment of protein kinase-mediated conditions, such as any of the conditions described above. In one embodiment, the protein kinase-mediated condition is characterized by undesired angiogenesis, edema, or stromal deposition. For example, the condition can be one or more more ulcers, such as ulcers caused by bacterial or fungal infections, Mooren ulcers and ulcerative colitis. The condition can also be due to a microbial infection, such as Lyme disease, sepsis, septic shock or infections by Herpes simplex, Herpes Zoster, human immunodeficincy virus, protozoa, toxoplasmosis or parapoxvirus; an angiogenic disorders, such as von Hippel Lindau disease, polycystic kidney disease, pemphigoid, Paget's disease and psoriasis; a reproductive condition, such as endometriosis, ovarian hyperstimulation syndrome, preeclampsia or
menometrorrhagia; a fibrotic and edemic condition, such as sarcoidosis, fibrosis, cirrhosis, thyroiditis, hyperviscosity syndrome systemic, Osier- Weber-Rendu disease, chronic occlusive pulmonary disease, asthma, and edema following burns, trauma, radiation, stroke, hypoxia or ischemia; or an iriflammatory/irnmunologic condition, such as systemic lupus, chronic inflammation, glomerulonephritis, synovitis, inflammatory bowel disease, Crohn's disease, rheumatoid arthritis, osteoarthritis, multiple sclerosis and graft rejection. Suitable protein kinase-mediated conditions also include sickle cell anaemia, osteoporosis, osteopetrosis, tumor-induced hypercalcemia and bone metastases. Additional protein kinase- mediated conditions which can be treated by the method of the present invention include ocular conditions such as ocular and macular edema, ocular neovascular disease, scleritis, radial keratotomy, uveitis, vitritis, myopia, optic pits, chronic retinal detachment, post-laser complications, conjunctivitis, Stargardt's disease and Eales disease, in addition to retinopathy and macular degeneration.
The compounds of the present invention are also useful in the treatment of cardiovascular conditions such as atherosclerosis, restenosis, vascular occlusion and carotid obstructive disease.
The compounds of the present invention are also useful in the treatment of cancer related indications such as solid tumors, sarcomas (especially Ewing's sarcoma and osteosarcoma), retinoblastoma, rhabdomyosarcomas, neuroblastoma, hematopoietic malignancies, including leukaemia and lymphoma, tumor-induced pleural or pericardial effusions, and malignant ascites.
The compounds of the present invention are also useful in the treatment of Crow-Fukase (POEMS) syndrome and diabetic conditions such as glaucoma, diabetic retinopathy and microangiopathy.
The Src, Tec, Jak, Map, Csk, NFKB and Syk families of kinases play pivotal roles in the regulation of immune function. The Src family currently includes Fyn, Lck, Fgr, Fes, Lyn, Src, Yrk, Fyk, Yes, Hck, and Blk. The Syk family is currently understood to include only Zap and Syk. The TEC family includes Tec, Btk, Rlk and Itk. The Janus family of kinases is involved in the transduction of growth factor and proinflammatory cytokine signals through a numlrer of receptors. Although BTK and ITK, members of the Tec family of kinases, play a less well understood role in immunobiology, their modulation by an inhibitor may prove therapeutically beneficial. The Csk family is currently understood to include Csk and Chk. The kinases RIP, IRAK-1, IRAK-2, NIK, p38 MAP kinases, Jnk, IKK-1 and IKK-2 are involved in the signal transduction pathways for key pro-inflammatory cytokines, such as TNF and IL-1. By virtue of their ability to inhibit one or more of these kinases, compounds of formula I may function as immunomodulatory agents useful for the maintenance of allografts, the treatment of autoimmune disorders and treatment of sepsis and septic shock. Through their ability to regulate the migration or activation of T cells, B-cells, mast cells, monocytes and neutrophils, these compounds could be used to treat such autoimmune diseases and sepsis. Prevention of transplant rejection, either host versus graft for solid organs or graft versus host for bone marrow, are limited by the toxicity of currently available
immunosuppressive agents and would benefit from an efficacious drug with improved therapeutic index. Gene targeting experiments have demonstrated the essential role of Src in the biology of osteoclasts, the cells responsible for bone resorption. Compounds of formula I, through their ability to regulate Src, may also be useful in the treatment of osteoporosis, osteopetrosis, Paget's disease, tumor-induced hypercalcemia and in the treatment of bone metastases.
A number of protein kinases have been demonstrated to be protooncogenes. Chromosome breakage (at the Itk kinase break point on chromosome 5),
translocation as in the case of the Abl gene with BCR (Philadelphia chromosome), truncation in instances such as c-Kit or EGFR, or mutation (e.g., Met) result in the creation of dysregulated proteins converting them from protooncogene to oncogene products. In other tumors, oncogenesis is driven by an autocrine or paracrine ligand/growth factor receptor interactions. Members of the src-family kinases are typically involved in downstream signal transduction thereby potentiating the oncogenesis and themselves may become oncogenic by over-expression or mutation. By inhibiting the protein kinase activity of these proteins the disease process may be disrupted. Vascular restenosis may involve FGF and/or PDGF - promoted smooth muscle and endothelial cell proliferation. The ligand stimulation of FGFR, PDGFR, IGF1-R and c-Met in vivo is proangiogenic, and potentiates angiogenesis dependent disorders. Inhibition of FGFr, PDGFr , c-Met, or IGF1-R kinase activities individually or in combination may be an efficacious strategy for inhibiting these phenomena. Thus compounds of formula I which inhibit the kinase activity of normal or aberrant c-kit, c-met, c-fins, src-family members, EGFr, erbB2, erbB4, BCR- Abl, PDGFr, FGFr, IGF1-R and other receptor or cytosolic tyrosine kinases may be of value in the treatment of benign and neoplastic proliferative diseases.
In many pathological conditions (for example, solid primary tumors and metastases, Kaposi's sarcoma, rheumatoid arthritis, blindness due to inappropriate ocular neovascularization, psoriasis and atherosclerosis) disease progression is contingent upon persistent angiogenesis. Polypeptide growth factors often produced by the disease tissue or associated inflammatory cells, and their corresponding endothelial cell specific receptor tyrosine kinases (e.g., KDR VEGFR-2, Flt-l/VEGFR-1, Tie-2/Tek and Tie) are essential for the stimulation of endothelial cell growth, migration, organization, differentiation and the establishment of the requisite new functional vasculature. As a result of the vascular permeability factor activity of VEGF in mediating vascular hyperpermeability, VEGF-stimulation of a VEGFR kinase is also believed to play an important role in the formation of tumor ascites, cerebral and pulmonary edema, pleural and pericardial effusions, delayed-type hypersensitivity reactions, tissue edema and organ dysfunction following trauma, burns, ischemia, diabetic complications, endometriosis, adult respiratory distress syndrome (ARDS), post-cardiopuhnonary bypass-related hypotension and hyperpermeability, and ocular edema leading to glaucoma or blindness due to inappropriate neovascularization. In addition to VEGF, recently identified VEGF-C and VEGF-D, and virally-encoded VEGF-E or HIV-Tat protein can also cause a vascular hyperpermeability response through the stimulation of a VEGFR kinase. KDR/VEGFR-2 and/or Tie-2 are expressed also in a select population of hematopoietic stem cells. Certain members of this population are pluripotent in nature and can be stimulated with growth factors to differentiate into endothelial cells and participate in vasculogenetic angiogenic processes. For this reason these have been called Endothelial Progenitor Cells (EPCs) (J Clin. Investig. 103 : 1231 -1236 (1999)). In some progenitors, Tie-2 may play a role in their recruitment, adhesion, regulation and differentiation (Blood , 4317-4326 (1997)). Certain agents according to formula I capable of blocking the kinase activity of endothelial cell specific kinases could therefore inhibit disease progression involving these situations.
Vascular destabilization of the antagonist ligand of Tie-2 (Ang2) is believed to induce an unstable "plastic" state in the endothelium. In the presence of high VEGF levels a robust angiogenic response may result; however, in the absence of VEGF or a VEGF-related stimulus, frank vessel regression and endothelial apoptosis can occur (Genes and Devel. 13: 1055-1066 (1999)). In an analogous manner a Tie-2 kinase inhibitor can be proangiogenic or antiangiogenic in the presence or absence of a VEGF-related stimulus, respectively. Hence, Tie-2 inhibitors can be employed with appropriate proangiogenic stimuli, such as VEGF, to promote therapeutic angiogenesis in situations such as wound healing, infarct and ischemia.
The compounds of formula I or a salt thereof or pharmaceutical compositions containing a therapeutically effective amount thereof may be used in the treatment of protein kinase-mediated conditions, such as benign and neoplastic proliferative diseases and disorders of the immune system, as described above. For example, such diseases include autoimmune diseases, such as rheumatoid arthritis, thyroiditis, type 1 diabetes, multiple sclerosis, sarcoidosis, inflammatory bowel disease, Crohn's disease, myasthenia gravis and systemic lupus erythematosus; psoriasis, organ transplant rejection (eg. kidney rejection, graft versus host disease), benign and neoplastic proliferative diseases, human cancers such as lung, breast, stomach, bladder, colon, pancreas, ovarian, prostate and rectal cancer and hematopoietic malignancies (leukemia and lymphoma), and diseases involving inappropriate vascularization for example diabetic retinopathy, retinopathy of prematurity, choroidal neovascularization due to age-related macular degeneration, and infantile hemangiomas in human beings. In addition, such inhibitors may be useful in the treatment of disorders involving VEGF mediated edema, ascites, effusions, and exudates, including for example macular edema, cerebral edema, acute lung injury and adult respiratory distress syndrome (ARDS).
The compounds of the present invention may also be useful in the
prophylaxis of the above diseases.
It is envisaged that the disorders listed above are mediated to a significant extent by protein tyrosine kinase activity involving the VEGF receptors (e.g. KDR, Flt-1 and/or Tie-2). By inhibiting the activity of these receptor tyrosine kinases, the progression of the listed disorders is inhibited because the angiogenic component of the disease state is severely curtailed. The action of the compounds of this invention, by their selectivity for specific tyrosine kinases, result in a mimmization of side effects that would occur if less selective tyrosine kinase inhibitors were used.

In another aspect the present invention provides compounds of formula I as defined initially above for use as medicaments, particularly as inhibitors of protein kinase activity for example tyrosine kinase activity, serine kinase activity and threonine kinase activity. In yet another aspect the present invention provides the use of compounds of formula I as defined initially above in the manufacture of a medicament for use in the inhibition of protein kinase activity.
Physiologically acceptable salts can refer to those salts which retain the biological effectiveness and properties of the free bases and which are obtained by reaction with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid or organic acids such as sulfonic acid, carboxylic acid, organic phosphoric acid, methanesulfonic acid, ethanesulfonic acid, p- toluenesulfonic acid, salicylic acid, lactic acid, tartaric acid and the like.
"Alkyl" refers to a saturated aliphatic hydrocarbon, including straight-chain and branched-chain groups having 1 to 6 carbons or cyclic hydrocarbons having 3 to 6 carbons.
"Alkoxy" refers to an "O-alkyl" group, where "alkyl" is defined as described ' above.

Phamaceutical Formulations
The compounds of this invention can be administered to a human patient by themselves or in pharmaceutical compositions where they are mixed with suitable carriers or excipient(s) at doses to treat or ameliorate vascular hyperpermeability, edema and associated disorders. Mixtures of these compounds can also be administered to the patient as a simple mixture or in suitable formulated
pharmaceutical compositions. A therapeutically effective dose further refers to that amount of the compound or compounds sufficient to result in the prevention or attenuation of inappropriate neovascularization, progression of hyperproliferative disorders, edema, VEGF-associated hyperpermeability and/or VEGF-related hypotension. Techniques for formulation and administration of the compounds of the instant application may be found in "Remington's Pharmaceutical Sciences," Mack Publishing Co., Easton, PA, latest edition.

Routes of Administration
Suitable routes of administration may, for example, include oral, eyedrop, rectal, transmucosal, topical, or intestinal administration; parenteral delivery, including intramuscular, subcutaneous, intramedullary injections, as well as intrathecal, direct intraventricular, intravenous, intraperitoneal, intranasal, or intraocular injections.
Alternatively, one may administer the compound in a local rather than a systemic manner, for example, via injection of the compound directly into an edematous site, often in a depot or sustained release formulation.
Furthermore, one may administer the drug in a targeted drug delivery system, for example, in a liposome coated with endothelial cell-specific antibody.

Composition/Formulation
The pharmaceutical compositions of the present invention may be
manufactured in a manner that is itself known, e.g., by means of conventional ' mixing, dissolving, granulating, dragee-making, levigating, emulsifying,
encapsulating, entrapping or lyophilizing processes.
Pharmaceutical compositions for use in accordance with the present invention thus may be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen.
For injection, the agents of the invention may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiological saline buffer. For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art.
For oral administration, the compounds can be formulated readily by combining the active compounds with pharmaceutically acceptable carriers well known in the art. Such carriers enable the compounds of the invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a patient to be treated.
Pharmaceutical preparations for oral use can be obtained by combining the active compound with a solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium
carboxymethylcellulose, and/or polyvinylpynolidone (PVP). If desired,
disintegrating agents may be added, such as the cross-linked polyvinyl pyπolidone, agar, or alginic acid or a salt thereof such as sodium alginate.
Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyπolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures.
Dyestuffs or pigments may be added to the tablets or dragee coatings for
identification or to characterize different combinations of active compound doses.
Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. La soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. All formulations for oral administration should be in dosages suitable for such administration.
For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.

For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, xrichlorofluoromethane,
dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.
The compounds can be formulated for parenteral administration by injection, e.g. bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g.in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.
Pharmaceutical formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes.
Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.
Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.
In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly or by intramuscular injection). Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
An example of a pharmaceutical carrier for the hydrophobic compounds of the invention is a cosolvent system comprising benzyl alcohol, a nonpolar surfactant, a water-miscible organic polymer, and an aqueous phase. The cosolvent system may be the VPD co-solvent system. VPD is a solution of 3% w/v benzyl alcohol, 8% w/v of the nonpolar surfactant polysorbate 80, and 65% w/v polyethylene glycol 300, made up to volume in absolute ethanol. The VPD co-solvent system
(VPD:5W) consists of VPD diluted 1:1 with a 5% dextrose in water solution. This co-solvent system dissolves hydrophobic compounds well, and itself produces low toxicity upon systemic administration. Naturally, the proportions of a co-solvent system may be varied considerably without destroying its solubility and toxicity characteristics. Furthermore, the identity of the co-solvent components may be varied: for example, other low-toxicity nonpolar surfactants may be used instead of polysorbate 80; the fraction size of polyethylene glycol may be varied; other biocompatible polymers may replace polyethylene glycol, e.g. polyvinyl
pynolidone; and other sugars or polysaccharides may substitute for dextrose.
Alternatively, other delivery systems for hydrophobic pharmaceutical compounds may be employed. Liposomes and emulsions are well known examples of delivery vehicles or carriers for hydrophobic drugs. Certain organic solvents such as dimethysulfoxide also may be employed, although usually at the cost of greater toxicity. Additionally, the compounds may be delivered using a sustained-release system, such as semipermeable matrices of solid hydrophobic polymers containing the therapeutic agent. Various sustained-release materials have been established and are well known by those skilled in the art. Sustained-release capsules may, depending on their chemical nature, release the compounds for a few weeks up to over 100 days. Depending on the chemical nature and the biological stability of the therapeutic reagent, additional strategies for protein stabilization may be employed.

The pharmaceutical compositions also may comprise suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include but are not limited to calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.
Many of the compounds of the invention may be provided as salts with pharmaceutically compatible counterions. Pharmaceutically compatible salts may be formed with many acids, including but not limited to hydrochloric, sulfuric, acetic, lactic, tartaric, malic, succinic, etc. Salts tend to be more soluble in aqueous or other pro tonic solvents than are the conesponding free base forms.

Effective Dosage
Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained in an effective amount to achieve its intended purpose. More specifically, a therapeutically effective amount means an amount effective to prevent development of or to alleviate the existing symptoms of the subject being treated. Determination of the effective amounts is well within the capability of those skilled in the art.
For any compound used in the method of the invention, the therapeutically effective dose can be estimated imtially from cellular assays. For example, a dose can be formulated in cellular and animal models to achieve a circulating
concentration range that includes the IC50 as determined in cellular assays (i.e., the concentration of the test compound which achieves a half-maximal inhibition of a given protein kinase activity). In some cases it is appropriate to determine the IC50 in the presence of 3 to 5% serum albumin since such a determination approximates the binding effects of plasma protein on the compound. Such information can be used to more accurately determine useful doses in humans. Further, the most prefened compounds for systemic administration effectively inhibit protein kinase signaling in intact cells at levels that are safely achievable in plasma.
A therapeutically effective dose refers to that amount of the compound that results in amelioration of symptoms in a patient. Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the maximum tolerated dose (MTD) and the ED50 (effective dose for 50% maximal response). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between MTD and ED50. Compounds which exhibit high therapeutic indices are prefened. The data obtained from these cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. (See e.g. Fingl et al, 1975, in "The Pharmacological Basis of Therapeutics", Ch. 1 pi). In the treatment of crises, the administration of an acute bolus or an infusion approaching the MTD may be required to obtain a rapid response.
Dosage amount and interval may be adjusted individually to provide plasma levels of the active moiety which are sufficient to maintain the kinase modulating effects, or minimal effective concentration (MEC). The MEC will vary for each compound but can be estimated from in vitro data; e.g. the concentration necessary to achieve 50-90% inhibition of protein kinase using the assays described herein.

Dosages necessary to achieve the MEC will depend on individual characteristics and route of administration. However, HPLC assays or bioassays can be used to determine plasma concentrations.
Dosage intervals can also be determined using the MEC value. Compounds should be admimstered using a regimen which maintains plasma levels above the MEC for 10-90% of the time, preferably between 30-90% and most preferably between 50-90% until the desired amelioration of symptoms is achieved. In cases of local administration or selective uptake, the effective local concentration of the drugmay not be related to plasma concentration.
The amount of composition administered will, of course, be dependent on the subject being treated, on the subject's weight, the severity of the affliction, the manner of administration and the judgment of the prescribing physician.
Packaging
The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration.

Compositions comprising a compound of the invention formulated in a compatible pharmaceutical carrier may also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition.
In some formulations it may be beneficial to use the compounds of the present invention in the form of particles of very small size, for example as obtained by fluid energy milling.
The use of compounds of the present invention in the manufacture of pharmaceutical compositions is illustrated by the following description. In this description the term "active compound" denotes any compound of the invention but particularly any compound which is the final product of one of the preceding

Examples.

a) Capsules

In the preparation of capsules, 10 parts by weight of active compound and 240 parts by weight of lactose can be de-aggregated and blended. The mixture can be filled into hard gelatin capsules, each capsule containing a unit dose or part of a unit dose of active compound.

b) Tablets

Tablets can be prepared from the following ingredients.
Parts by weight
Active compound 10
Lactose 190 Maize starch 22
Polyvinylpynolidone 10
Magnesium stearate 3
The active compound, the lactose and some of the starch can be de-aggregated, blended and the resulting mixture can be granulated with a solution of the polyvinyl- pyπolidone in ethanol. The dry granulate can be blended with the magnesium stearate and the rest of the starch. The mixture is then compressed in a tabletting machine to give tablets each containing a unit dose or a part of a unit dose of active compound.

c) Enteric coated tablets

Tablets can be prepared by the method described in (b) above. The tablets can be enteric coated in a conventional manner using a solution of 20% cellulose acetate phthalate and 3% diethyl phthalate in ethanohdichloromethane (1:1).

d) Suppositories
In the preparation of suppositories, 100 parts by weight of active compound can be incorporated in 1300 parts by weight of triglyceride suppository base and the mixture formed into suppositories each containing a therapeutically effective amount of active ingredient.
In the compositions of the present invention the active compound may, if desired, be associated with other compatible pharmacologically active ingredients. For example, the compounds of this invention can be admimstered in combination with one or more additional pharmaceutical agents that inhibit or prevent the production of VEGF or angiopoietins, attenuate intracellular responses to VEGF or angiopoietins, block intracellular signal transduction, inhibit vascular
hyperpermeability, reduce inflammation, or inhibit or prevent the formation of edema or neovascularization. The compounds of the invention can be admimstered prior to, subsequent to or simultaneously with the additional pharmaceutical agent, whichever course of administration is appropriate. The additional pharmaceutical agents include but are not limited to anti-edemic steroids, NSAIDS, ras inhibitors, anti-TNF agents, anti-ILl agents, antihistamines, PAF-antagonists, COX-1 inhibitors, COX-2 inhibitors, NO synthase inhibitors, Akt/PTB inhibitors, IGF-IR inhibitors, PKC inhibitors and PI3 kinase inhibitors. The compounds of the invention and the additional pharmaceutical agents act either additively or synergistically. Thus, the administration of such a combination of substances that inhibit angiogenesis, vascular hyperpermeability and/or inhibit the formation of edema can provide greater relief from the deletrious effects of a hyperproliferative disorder, angiogenesis, vascular hyperpermeability or edema than the administration of either substance alone. In the treatment of malignant disorders combinations with antiproliferative or cytotoxic chemotherapies or radiation are anticipated.
The present invention also comprises the use of a compound of formula I as a medicament.
A further aspect of the present invention provides the use of a compound of formula I or a salt thereof in the manufacture of a medicament for treating vascular hyperpermeability, angiogenesis-dependent disorders, proliferative diseases and/or disorders of the immune system in mammals, particularly human beings.
The present invention also provides a method of treating vascular
hyperpermeability, inappropriate neovascularization, proliferative diseases and/or disorders of the immune system which comprises the administration of a
therapeutically effective amount of a compound of formula I to a mammal, particularly a human being, in need thereof.
The in vitro potency of compounds in inhibiting these protein kinases may be determined by the procedures detailed below.
The potency of compounds can be determined by the amount of inhibition of the phosphorylation of an exogenous substrate (e.g., synthetic peptide (Z. Songyang et al, Nature. 373:536-539)) by a test compound relative to control.

KDR Tyrosine Kinase Production Using Baculovirus System:
The coding sequence for the human KDR intra-cellular domain (aa789-1354) was generated through PCR using cDNAs isolated from HUVEC cells. A poly-His6 sequence was introduced at the N-terminus of this protein as well. This fragment was cloned into transfection vector pVL1393 at the Xba 1 and Not 1 site.
Recombinant baculovirus (BV) was generated through co-transfection using the BaculoGold Transfection reagent (PharMingen). Recombinant BV was plaque purified and verified through Western analysis. For protein production, SF-9 cells were grown in SF-900-II medium at 2 x 106/ml, and were infected at 0.5 plaque forming units per cell (MOI). Cells were harvested at 48 hours post infection.

Purification of KDR
SF-9 cells expressing (His)6KDR(aa789-1354) were lysed by adding 50 ml of Triton X-100 lysis buffer (20 mM Tris, pH 8.0, 137 mM NaCl, 10% glycerol, 1% Triton X-100, ImM PMSF, lOμg/ml aprotinin, 1 μg/ml leupeptin) to the cell pellet from IL of cell culture. The lysate was centrifuged at 19,000 rpm in a Sorval SS-34 rotor for 30 min at 4°C. The cell lysate was applied to a 5 ml NiCl2 chelating sepharose column, equilibrated with 50 mM HEPES, ρH7.5, 0.3 M NaCl. KDR was eluted using the same buffer containing 0.25 M imidazole. Column fractions were analyzed using SDS-PAGE and an ELISA assay (below) which measures kinase activity. The purified KDR was exchanged into 25mM HEPES, ρH7.5, 25mM NaCl, 5 mM DTT buffer and stored at -80 °C.

Human Tie-2 Kinase Production and Purification
The coding sequence for the human Tie-2 intra-cellular domain (aa775-l 124) was generated through PCR using cDNAs isolated from human placenta as a template. A poly-His6 sequence was introduced at the N-terminus and this construct was cloned into transfection vector pVL 1939 at the Xba 1 and Not 1 site.
Recombinant BV was generated through co-transfection using the BaculoGold Transfection reagent (PharMingen). Recombinant BV was plaque purified and verified through Western analysis. For protein production, SF-9 insect cells were grown in SF-900-II medium at 2 x 106/ml, and were infected at MOI of 0.5.
Purification of the His-tagged kinase used in screening was analogous to that described for KDR.

Human Flt-1 Tyrosine Kinase Production and Purification
The baculoviral expression vector pVL 1393 (PharMingen, Los Angeles, CA) was used. A nucleotide sequence encoding poly-His6 was placed 5' to the nucleotide region encoding the entire intracellular kinase domain of human Flt-1 (amino acids 786-1338). The nucleotide sequence encoding the kinase domain was generated through PCR using cDNA libraries isolated from HUVEC cells. The histidine residues enabled affinity purification of the protein as a manner analogous to that for KDR and ZAP70. SF-9 insect cells were infected at a 0.5 multiplicity and harvested 48 hours post infection.

EGFR Tyrosine Kinase Source
EGFR was purchased from Sigma (Cat # E-3641 ; 500 units/50 μl) and the EGF ligand was acquired from Oncogene Research Products/Calbiochem (Cat # PF011-100).

Expression of ZAP70
The baculoviral expression vector used was pVL1393. (Pharmingen, Los Angeles, Ca.) The nucleotide sequence encoding amino acids M(H)6 LVPRgS was placed 5' to the region encoding the entirety of ZAP70 (amino acids 1-619). The nucleotide sequence encoding the ZAP70 coding region was generated through PCR using cD A libraries isolated from Jurkat immortalized T-cells. The histidine residues enabled affinity purification of the protein (vide infra). The LVPRgS bridge constitutes a recognition sequence for proteolytic cleavage by thrombin, enabling removal of the affinity tag from the enzyme. SF-9 insect cells were infected at a multiplicity of infection of 0.5 and harvested 48 hours post infection.

Extraction and purification of ZAP70 SF-9 cells were lysed in a buffer consisting of 20 mM Tris, pH 8.0, 137 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1 μg/ml leupeptin, 10 μg/ml aprotinin and 1 mM sodium orthovanadate. The soluble lysate was applied to a chelating sepharose HiTrap column (Pharmacia) equilibrated in 50 mM HEPES, pH 7.5, 0.3 M NaCl. Fusion protein was eluted with 250 mM imidazole. The enzyme was stored in buffer containing 50 mM HEPES, pH 7.5, 50 mM NaCl and 5 mM DTT.

Protein kinase source
Lck, Fyn, Src, Blk, Csk, and Lyn, and truncated forms thereof may be commercially obtained ( e.g. from Upstate Biotechnology Inc. (Saranac Lake, N,Y) and Santa Cruz Biotechnology Inc. (Santa Cruz, Ca.)) or purified from known natural or recombinant sources using conventional methods.

Enzyme Linked Immunosorbent Assay (ELISA) For PTKs
Enzyme linked immunosorbent assays (ELISA) were used to detect and measure the presence of tyrosine kinase activity. The ELISA were conducted according to known protocols which are described in, for example, Voller, et al, 1980, "Enzyme-Linked Immunosorbent Assay," In: Manual of Clinical Immunology, 2d ed., edited by Rose and Friedman, pp 359-371 Am. Soc. of Microbiology, Washington, D.C.
The disclosed protocol was adapted for determining activity with respect to a specific PTK. For example, prefened protocols for conducting the ELISA experiments is provided below. Adaptation of these protocols for determimng a compound's activity for other members of the receptor PTK family, as well as non-receptor tyrosine kinases, are well within the abilities of those in the art. For purposes of determining inhibitor selectivity, a universal PTK substrate (e.g., random copolymer of poly(Glu4 Tyr), 20,000-50,000 MW) was employed together with ATP (typically 5 μM) at concentrations approximately twice the apparent Km in the assay.
The following procedure was used to assay the inhibitory effect of compounds of this invention on KDR, Flt-1, Tie-2, EGFR, FGFR, PDGFR, IGF-1- R, c-Met, Lck, Blk, Csk, Src, Lyn, Fyn and ZAP70 tyrosine kinase activity:

Buffers and Solutions:
PGTPoly (Glu,Tyr) 4:l
Store powder at -20°C. Dissolve powder in phosphate buffered saline (PBS) for

50mg/ml solution. Store 1ml aliquots at -20°C. When making plates dilute to 250μg/ml in Gibco PBS .
Reaction Buffer: lOOmM Hepes, 20mM MgCl2, 4mM MnCl2, 5mM DTT,
0.02%BSA, 200μM NaVO4, pH 7.10
ATP: Store aliquots of lOOmM at -20°C. Dilute to 20μM in water
Washing Buffer: PBS with 0.1% Tween 20
Antibody Diluting Buffer: 0.1 % bovine serum albumin (BSA) in PBS
TMB Substrate: mix TMB substrate and Peroxide solutions 9:1 just before use or use K-Blue Substrate from Neogen
Stop Solution: IM Phosphoric Acid

Procedure
1. Plate Preparation:
Dilute PGT stock (50mg/ml, frozen) in PBS to a 250μg/ml. Add 125μl per well of Corning modified flat bottom high affinity ELISA plates (Corning #25805-96). Add 125μl PBS to blank wells. Cover with sealing tape and incubate overnight 37°C. Wash lx with 250μl washing buffer and dry for about 2hrs in 37°C dry incubator. Store coated plates in sealed bag at 4°C until used.

2. Tyrosine Kinase Reaction:
-Prepare inhibitor solutions at a 4x concentration in 20% DMSO in water.
-Prepare reaction buffer -Prepare enzyme solution so that desired units are in 50μl, e.g. for KDR make to 1 ng/μl for a total of 50ng per well in the reactions. Store on ice.
-Make 4x ATP solution to 20μM from lOOmM stock in water. Store on ice -Add 50μl of the enzyme solution per well (typically 5-50 ng enzyme/well depending on the specific activity of the kinase)
-Add 25μl 4x inhibitor
-Add 25μl 4x ATP for inhibitor assay
-Incubate for 10 minutes at room temperature
-Stop reaction by adding 50μl 0.05N HCI per well
-Wash plate

**Final Concentrations for Reaction: 5μM ATP, 5% DMSO
3. Antibody Binding
-Dilute lmg/ml aliquot of PY20-HRP (Pierce) antibody(a phosphotyrosine antibody)to 50ng/ml in 0.1 % BSA in PBS by a 2 step dilution (1 OOx, then 200x) -Add lOOμl Ab per well. Incubate 1 hr at room temp. Incubate lhr at 4C.
-Wash 4x plate
4. Color reaction
-Prepare TMB substrate and add lOOμl per well
-Monitor OD at 650nm until 0.6 is reached
-Stop with IM Phosphoric acid. Shake on plate reader.
-Read OD immediately at 450nm
Optimal incubation times and enzyme reaction conditions vary slightly with enzyme preparations and are determined empirically for each lot.

For Lck, the Reaction Buffer utilized was 100 mM MOPSO, pH 6.5, 4 mM MnCl2,

20 mM MgCl2, 5 mM DTT, 0.2% BSA, 200 mM NaVO4 under the analogous assay conditions.
Compounds of formula I may have therapeutic utility in the treatment of diseases involving both identified, including those not mentioned herein, and as yet unidentified protein tyrosine kinases which are inhibited by compounds of formula I.

All compounds exemplified herein significantly inhibit either FGFR, PDGFR, KDR, Tie-2, Lck, Fyn, Blk, Lyn or Src at concentrations of 50 micromolar or below.

Some compounds of this invention also significantly inhibit other tyrosine or serine/threonine kinases such as cdc2 (cdkl) at concentrations of 50 micromolar or below.

Cdc2 source
The human recombinant enzyme and assay buffer may be obtained commercially (New England Biolabs, Beverly, MA. USA) or purified from known natural or recombinant sources using conventional methods.

Cdc2 Assay
The protocol used was that provided with the purchased reagents with minor modifications. In brief, the reaction was carried out in a buffer consisting of 50mM Tris pH 7.5, lOOmM NaCl, ImM EGTA, 2mM DTT, 0.01% Brij, 5% DMSO and lOmM MgCl2 (commercial buffer) supplemented with fresh 300 μM ATP (31 μCi/ml) and 30 μg/ml histone type IIIss final concentrations. A reaction volume of 80μL, containing units of enzyme, was run for 20 minutes at 25 degrees C in the presence or absence of inhibitor. The reaction was terminated by the addition of 120 L of 10%) acetic acid. The substrate was separated from unincorporated label by spotting the mixture on phosphocellulose paper, followed by 3 washes of 5 minutes each with 75mM phosphoric acid. Counts were measured by a betacounter in the presence of liquid scintillant.

Certain compounds of this invention significantly inhibit cdc2 at concentrations below 50 uM.

PKC kinase source
The catalytic subunit of PKC may be obtained commercially (Calbiochem).

PKC kinase assay
A radioactive kinase assay was employed following a published procedure (Yasuda, I., IQrshimoto, A., Tanaka, S., Tominaga, M., Sakurai, A., Nishizuka, Y. Biochemical and Biophysical Research Communication 5: 166, 1220-1227 (1 90)). Briefly, all reactions were performed in a kinase buffer consisting of 50 mM Tris-HCl pH7.5, lOmM MgCl2, 2mM DTT, ImM EGTA, 100 μM ATP, 8 μM peptide, 5% DMSO and 33P ATP (8Ci/mM). Compound and enzyme were mixed in the reaction vessel and the reaction initiated by addition of the ATP and substrate mixture. Following termination of the reaction by the addition of 10 μL stop buffer (5 mM ATP in 75mM phosphoric acid), a portion of the mixture was spotted on phosphocellulose filters. The spotted samples were washed 3 times in 75 mM phosphoric acid at room temperature for 5 to 15 minutes. Incorporation of radiolabel was quantified by liquid scintillation counting.

Erk2 enzyme source
The recombinant murine enzyme and assay buffer may be obtained commercially (New England Biolabs, Beverly MA. USA) or purified from known natural or recombinant sources using conventional methods.

Erk2 enzyme assay
In brief, the reaction was carried out in a buffer consisting of 50 mM Tris pH 7.5, ImM EGTA, 2mM DTT, 0.01% Brij, 5% DMSO and 10 mM MgCl2
(commercial buffer) supplemented with fresh 100 μM ATP (31 μCi/ml) and 30μM myelin basic protein under conditions recommended by the supplier. Reaction volumes and method of assaying incorporated radioactivity were as described for the PKC assay (vide supra).

In Vitro Models for T-cell Activation
Upon activation by mitogen or antigen, T-cells are induced to secrete IL-2, a growth factor that supports their subsequent proliferative phase. Therefore, one may measure either production of IL-2 from or cell proliferation of, primary T-cells or appropriate T-cell lines as a suπogate for T-cell activation. Both of these assays are well described in the literature and their parameters well documented (in Cunent Protocols in Immunology, Vol 2, 7.10.1-7.11.2).
In brief, T-cells may be activated by co-culture with allogenic stimulator cells, a process termed the one-way mixed lymphophocyte reaction. Responder and stimulator peripheral blood mononuclear cells are purified by Ficoll-Hypaque gradient (Pharmacia) per directions of the manufacturer. Stimulator cells are mitotically inactivated by treatment with mitomycin C (Sigma) or gamma iπadiation. Responder and stimulator cells are co-cultured at a ratio of two to one in the presence or absence of the test compound. Typically 105responders are mixed with 5 x 104 stimulators and plated (200 μl volume) in a U bottom microtiter plate (Costar Scientific). The cells are cultured in RPMI 1640 supplemented with either heat inactivated fetal bovine serum (Hyclone Laboratories) or pooled human AB serum from male donors, 5 x 10"5M 2mercaptoethanol and 0.5% DMSO, The cultures are pulsed with 0.5 μCi of 3H thymidine (Amersham) one day prior to harvest (typically day three). The cultures are harvested (Betaplate harvester, Wallac) and isotope uptake assessed by liquid scintillation (Betaplate, Wallac).
The same culture system may be used for assessing T-cell activation by measurement of IL-2 production. Eighteen to twenty-four hours after culture initiation, the supernatants are removed and the IL-2 concentration is measured by ELISA (R and D Systems) following the directions of the manufacturer.

In-vivo Models of T-Cell Activation
The in vivo efficacy of compounds can be tested in animal models known to directly measure T-cell activation or for which T-cells have been proven the effectors. T-cells can be activated in vivo by ligation of the constant portion of the T-cell receptor with a monoclonal anti-CD3 antibody (Ab). In this model, BALB/c mice are given lOμg of anti-CD3 Ab intraperitoneally two hours prior to exsanguination. Animals to receive a test drug are pre-treated with a single dose of the compound one hour prior to anti-CD3 Ab administration. Serum levels of the proinflammatory cytokines interferon-γ (IFN- γ) and tumor necrosis
factor-α(TNF-α), indicators of T-cell activation, are measured by ELISA. A similar model employs in vivo T-cell priming with a specific antigen such as keyhole limpet hemocyanin (KLH) followed by a secondary in vitro challenge of draining lymph node cells with the same antigen. As previously, measurement of cytokine production is used to assess the activation state of the cultured cells. Briefly, C57BL/6 mice are immunized subcutaneously with 100 μg KLH emulsified in complete Freund's adjuvant (CFA) on day zero. Animals are pre-treated with the compound one day prior to immunization and subsequently on days one, two and three post irnmumzation. Draining lymph nodes are harvested on day 4 and their cells cultured at 6 x 106per ml in tissue culture medium (RPMI 1640 supplemented with heat inactivated fetal bovine serum (Hyclone Laboratories) 5 x 10"5M
2-mercaptoethanol and 0.5% DMSO) for both twenty-four and forty-eight hours. Culture supernatants are then assessed for the autocrine T-cell growth factor Interleukin-2 (IL-2) and/or IFN-γ levels by ELISA.
Lead compounds can also be tested in animal models of human disease. These are exemplified by experimental auto-immune encephalomyelitis (EAE) and collagen-induced arthritis (CIA). EAE models which mimic aspects of human multiple sclerosis have been described in both rats and mice (reviewed FASEB J. 5:2560-2566, 1991; murine model: Lab. Invest. 4(3):278, 1981; rodent model: J. Immunol 146(4):1163-8, 1991 ). Briefly, mice or rats are immunized with an emulsion of myelin basic protein (MBP), or neurogenic peptide derivatives thereof, and CFA. Acute disease can be induced with the addition of bacterial toxins such as bordetella pertussis. Relapsing/remitting disease is induced by adoptive transfer of T-cells from MBP/ peptide immunized animals.
CIA may be induced in DBA/1 mice by immunization with type II collagen (J. Immunol: 142(7):2237-2243). Mice will develop signs of arthritis as early as ten days following antigen challenge and may be scored for as long as ninety days after immunization. In both the EAE and CIA models, a compound may be admimstered either prophylactically or at the time of disease onset. Efficacious drugs should reduce severity and/or incidence.
Certain compounds of this invention which inhibit one or more angiogenic receptor PTK, and/or a protein kinase such as lck involved in mediating
inflammatory responses can reduce the severity and incidence of arthritis in these models.
Compounds can also be tested in mouse allograft models, either skin
(reviewed in Ann. Rev. Immunol., 10:333-58, 1992; Transplantation: 57(12):
1701-17D6, 1994) or heart (Am.J.Anat: 113:273, 1963). Briefly, full thickness skin grafts are transplanted from C57BL/6 mice to BALB/c mice. The grafts can be examined daily, beginning at day six, for evidence of rejection. In the mouse neonatal heart transplant model, neonatal hearts are ectopically transplanted from C57BL/6 mice into the ear pinnae of adult CBA/J mice. Hearts start to beat four to seven days post transplantation and rejection may be assessed visually using a dissecting microscope to look for cessation of beating.

Cellular Receptor PTK Assays
The following cellular assay was used to determine the level of activity and effect of the different compounds of the present invention on KDRVEGFR2.
Similar receptor PTK assays employing a specific ligand stimulus can be designed along the same lines for other tyrosine kinases using techniques well known in the art.
VEGF-induced KDR Phosphorylation in Human Umbilical Vein Endothelial

Cells (HUVEC) as Measured by Western Blots:
1. HUVEC cells (from pooled donors) were purchased from Clonetics

(San Diego, CA) and cultured according to the manufacturer directions. Only early passages (3-8) were used for this assay. Cells were cultured in 100 mm dishes (Falcon for tissue culture; Becton Dickinson; Plymouth, England) using complete

EBM media (Clonetics).

2. For evaluating a compound's inhibitory activity, cells were trypsinized and seeded at 0.5-1.0 x 10s cells/well in each well of 6-well cluster plates (Costar; Cambridge, MA).
3. 3-4 days after seeding, plates were 90-100% confluent. Medium was removed from all the wells, cells were rinsed with 5-10ml of PBS and incubated 18- 24h with 5ml of EBM base media with no supplements added (i.e., serum starvation).
4. Serial dilutions of inhibitors were added in 1ml of EBM media (25μM, 5μM, or lμM final concentration to cells and incubated for one hour at 37 C. Human recombinant VEGFι65 ( R & D Systems) was then added to all the wells in 2 ml of EBM medium at a final concentration of 50ng/ml and incubated at 37 C for 10 minutes. Control cells untreated or treated with VEGF only were used to assess background phosphorylation and phosphorylation induction by VEGF.
All wells were then rinsed with 5-10ml of cold PBS containing ImM Sodium Orthovanadate (Sigma) and cells were lysed and scraped in 200μl of RIP A buffer (50mM Tris-HCl) pH7, 150mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, ImM EDTA) containing protease inhibitors (PMSF ImM, aprotinin lμg/ml, pepstatin lμg/ml, leupeptin lμg/ml, Na vanadate ImM, Na fluoride ImM) and lμg/ml of Dnase (all chemicals from Sigma Chemical Company, St Louis, MO). The lysate was spun at 14,000 rpm for 30min, to eliminate nuclei.
Equal amounts of proteins were then precipitated by addition of cold (-20 C) Ethanol (2 volumes) for a minimum of 1 hour or a maximum of overnight. Pellets were reconstituted in Laemli sample buffer containing 5% -mercaptoethanol (BioRad; Hercules, CA) and boiled for 5min. The proteins were resolved by polyacrylamide gel electrophoresis (6%, 1.5mm Novex, San Deigo, CA) and transfened onto a nitrocellulose membrane using the Novex system. After blocking with bovine serum albumin (3%), the proteins were probed overnight with anti-KDR polyclonal antibody (C20, Santa Cruz Biotechnology; Santa Cruz, CA) or with anti-phosphotyrosine monoclonal antibody (4G10, Upstate Biotechnology, Lake Placid, NY) at 4 C. After washing and incubating for 1 hour with HRP-conjugated F(ab)2 of goat anti-rabbit or goat-anti-mouse IgG the bands were visualized using the emission chemiluminescience (ECL) system (Amersham Life Sciences, Arlington Height, IL). Certain examples of the present invention significantly inhibit cellular VEGF-induced KDR tyrosine kinase phosphorylation at concentrations of less than 50 μM.

In vivo Uterine Edema Model
This assay measures the capacity of compounds to inhibit the acute increase in uterine weight in mice which occurs in the first few hours following estrogen stimulation. This early onset of uterine weight increase is known to be due to edema caused by increased permeability of uterine vasculature. Cullinan-Bove and Koss (Endocrinology (1993), 733:829-837) demonstrated a close temporal relationship of estrogen-stimulated uterine edema with increased expression of VEGF mRNA in the uterus. These results have been confirmed by the use of neutralizing monoclonal antibody to VEGF which significantly reduced the acute increase in uterine weight following estrogen stimulation (WO 97/42187). Hence, this system can serve as a model for in vivo inhibition of VEGF signalling and the associated
hyperpermeability and edema.

Materials: All hormones were purchased from Sigma (St. Louis, MO) or Cal Biochem (La Jolla, CA) as lyophilized powders and prepared according to supplier instructions.
Vehicle components (DMSO, Cremaphor EL) were purchased from Sigma (St.
Louis, MO).
Mice (Balb/c, 8-12 weeks old) were purchased from Taconic (Germantown, NY) and housed in a pathogen-free animal facility in accordance with institutional

Animal Care and Use Committee Guidelines.

Method:

Day 1 : Balb/c mice were given an intraperitoneal (i.p.) injection of 12.5 units of pregnant mare's serum gonadotropin (PMSG).
Day 3: Mice received 15 units of human chorionic gonadotropin

(hCG) i.p.
Day 4: Mice were randomized and divided into groups of 5-10. Test compounds were administered by i.p., i.v. or p.o. routes depending on solubility and vehicle at doses ranging from 1-100 mg/kg. Vehicle control group received vehicle only and two groups were left untreated.
Thirty minutes later, experimental, vehicle and 1 of the untreated groups were given an i.p. injection of 17 -estradiol (500 μg/kg). After 2-3 hours, the animals were sacrificed by CO2 inhalation. Following a midline incision, each uterus was isolated and removed by cutting just below the cervix and at the junctions of the uterus and oviducts. Fat and connective tissue were removed with care not to disturb the integrity of the uterus prior to weighing (wet weight). Uteri were blotted to remove fluid by pressing between two sheets of filter paper with a one liter glass bottle filled with water. Uteri were weighed following blotting (blotted weight). The difference between wet and blotted weights was taken as the fluid content of the uterus. Mean fluid content of treated groups was compared to untreated or vehicle treated groups. Significance was determined by Student's test. Non-stimulated control group was used to momtor estradiol response.
Results demonstrate that certain compounds of the present invention inhibit the formation of edema when administered systemically by various routes.
Certain compounds of this invention which are inhibitors of angiogenic receptor tyrosine kinases can also be shown active in a Matrigel implant model of neovascularization. The Matrigel neovascularization model involves the formation of new blood vessels within a clear "marble" of extracellular matrix implanted subcutaneously which is induced by the presence of proangiogenic factor producing tumor cells (for examples see: Passaniti, A., et al, Lab. Investig. (1992), 67(4), 519-528; Anal Rec. (1997), 249(1), 63-73; Int. J. Cancer (1995), 63(5), 694-701; Vase. Biol. (1995), 15(11), 1857-6). The model preferably runs over 3-4days and endpoints include macroscopic visual/image scoring of neovascularization, microscopic microvessel density determinations, and hemoglobin quantitation (Drabkin method) following removal of the implant versus controls from animals untreated with inhibitors. The model may alternatively employ bFGF or HGF as the stimulus.
Certain compounds of this invention which inhibit one or more oncogenic, protooncogenic, or proliferation-dependent protein kinases, or angiogenic receptor PTK also inhibit the growth of primary murine, rat or human xenograft tumors in mice, or inhibit metastasis in murine models.

EXAMPLES
Processes for the preparation of compounds of formula I will now be described. These processes form a further aspect of the present invention. The processes are preferably carried out at atmospheric pressure.
Compounds of formula I may be prepared by condensing a compound of formula



(H)

in which R,, R2, R3, L and ring A are as previously defined with formamide at a temperature in the range of 50 to 250°C optionally in the presence of a catalyst for example 4-dimethylaminopyridine.

Compounds of formula I may be prepared by reacting a compound of formula (HI)



(in)
wherein R,. is bromo or iodo bromo or iodo with one of the following compounds: R3B(OH)2, R3Sn(CH3)3 or a compound represented by formula IV




in which R3 is as defined above, in the presence of a catalyst for example palladium (0) compounds eg. Pd(PPh3)4.
Compounds of formula I in which R{ represents an alkyl group or an aralkyl group may be prepared by alkylating a compound of formula (V)



(V)

in which R2 and R3 are as previously defined with a compound of formula Ri ' in which Rj represents an alkyl group or an aralkyl group and X' represents a leaving group, for example halo, mesyloxy or tosyloxy.
Compounds of formula I in which Ri represents an optionally substituted cyclic ether, such as tefrahydrofuryl or tetrahydropyranyl, may be prepared by alkylating a compound of formula VI



(VI)

in which R2 and R3 are as previously defined with a compound of formula RχX5 in which X' is as previously defined and Rj is an optionally substituted cyclic ether. Compounds of formula I in which Ri represents cyclic ether, such as tefrahydrofuryl or tetrahydropyranyl, optionally substituted by formyl may be prepared by alkylating a compound of formula VI with a compound R]X in which Rl represents a cyclic ether substituted by a formyl group which has been protected, by a method known to those skilled in the art, for example by means of an acetal, (See for example Test. Letts. 30(46):6259-6262 (1989)) followed by deprotection. Compounds in which Rj represents a cyclic ether, such as tetrahydrofuryl or tetrahydropyranyl, substituted by an (optionally substituted amino)methyl group may be prepared by reductive amination of a compound in which Ri represents a cyclic ether substituted by formyl.
Compounds of formula I in which Ri represents optionally substituted furyl, thienyl or pynolyl may be prepared by reacting 4-chloro-5-iodo-7H-pynolo[2,3-d]pyrmιidine with the appropriate heteroarylboronic acid in the presence of a copper salt catalyst, for example copper (II) acetate in the presence of a solvent for the reactants, e.g. a halogenated solvent for example, dichloromethane, in the presence of a drying agent, for example 4A molecular sieves, in the presence of an organic base, e.g. triethylamine or pyridine, at a temperature in the range of 0-50°C, preferably at ambient temperature. (For conditions see Eet. Letts. (1998),
39:2942-2944 and references cited therein. This paper is incorporated herein by reference.) These compounds may be formulated by methods known to those skilled in the art to give compounds in which R\ represents furyl, thienyl or pynolyl substituted by formyl. The formyl group in these compounds may be productively aminated by methods known to those skilled in the art to give compounds in which Rl represents furyl, thienyl or pynolyl substituted by aminomethyl groups.
Alternatively intermediates in which Ri represents furyl, thienyl or pynolyl may be subjected to a Mannich reaction to give intermediates in which Rl represents furyl, thienyl or pynolyl substituted by an aminomethyl group.

Compounds of formula I may be prepared by reacting a compound of formula VTJ



VII

in which Rl9 R2, R3, L and ring A are as previously defined and Ry represents a leaving group, for example halo or phenoxy, with ammonia or an ammonium salt, for example ammonium acetate, at a temperature in the range of 15-250°C, preferably in a pressure vessel.
Compounds of formula I in which R2 represents chloro, bromo or iodo rnay be prepared by reacting a compound of formula Vffl



(vm)

in which Rl5 R3 L and ring A are as previously defined with a halogenating agent for example an iodinating agent, e.g. N-iodosuccinimide, or a brominating agent, e.g. N-bromosuccinimide, or a chlorinating agent, e.g. N-chlorosuccinimide.

Compounds of formula I in which -L-R3 represents -NHC(O)R3 may be prepared by reacting a compound of formula IX


IX

in which Rj, R2 and ring A are as previously defined and Y represents a protected amine, with a compound of formula R3COR_. in which R_ represents a leaving group, for example chloro. Alternatively compounds of formula IX in which Y represents halo, for example chloro, may be reacted with a compound of formula R3COR-. and the product reacted with ammonia to give a compound of formula I. Analogous methods may be used to prepare compounds of formula I in which -L-R3 is -NRSO2R3. Analogous methods may be used to prepare compound of formula I in which -L-R3 is -NRCO2-R3 or -NRCONR' . R and R' are as previously defined.
Compounds of formula I in which -L-R3 is -OSO2- may be prepared by reacting a compound of formula X



X in which ~R._, R2 and ring A are as previously defined with a compound of formula R4SOA.
Compounds of formula I may then be prepared from such intermediates following Scheme 2 or the alternative for Scheme 2, which is described later.
Compounds of formula II may be prepared as shown in Scheme 1 in which IPA represents propan-2-ol,

Scheme I


1) HJ RL IPA
or CH 3CN
2) HCl IPA


1) NaOCH2CH3/CH3CH2OH
2) NCCH2CN NaOCH2CH3,
CH3CH2OH, 50°C


It will be appreciated by those skilled in the art that compounds of fprmula I may be converted into other compounds of formula I by known chemical reactions. For example, an alkoxy group may be cleaved to give hydroxy, nitro groups may be reduced to amines, amines may be acylated or sulfonylated and N-acyl compounds may be hydrolyzed to amines. Compounds of formula I in which -L- is S may be oxidized to give compounds of formula I in which -L- represents SO and SO2, respectively, by methods known to those skilled in the art.
Compounds of formula m are commercially available or may be prepared by methods known to those skilled in the art.
Compounds of formula V in which R2 represents hydrogen may be prepared as shown in Scheme 2. The amino group may be protected prior to the final step and then deprotected after the final step of scheme 2 by methods known to those skilled in the art. Compounds of formula V in which R2 is other than hydrogen may be prepared by analogous methods. (see J. Med. Chem. (1990), 33, 1984.)

Scheme 2


Alternatively in Scheme 2, (ring A)-L-R3 may be coupled first, prior to amination. Alternatively a substituent Rj as defined previously may be present prior to carrying out either process.
Compounds of formula VTI, in which Ry is a -Cl, may be prepared as shown in Scheme 3.

Scheme 3

N- iodosuccinamide



Compounds in which (ring A)-L-R3 is absent may be prepared as in Scheme 4 and as described in J.Med. Chem., (1988), 37:390 and references cited therein. Compounds in which (ring A)-L-R3 is other than hydrogen may be prepared by analogous methods,



Compounds of formula VH may be prepared by coupling a 5-iodcPcompound in an analogous manner to that described for the preparation of compounds of
Scheme 4 formula V.
R, may be modified by the method depicted in Schemes 5 and 6. In Schemes 5 and 6 P represents a protecting group.
Scheme 5



" "R
Na(OAc)3BH, HoAc
C1CH2CH2C1


Scheme 6


It will be appreciated by those skilled in the art that in cases where a substituent is identical with, or similar to, a functional group which has been modified in one of the above processes that these substituents will require protection before the process is undertaken, followed by deprotection after the process.

Otherwise competing sidereactions will occur. Alternatively, another of the processes described above, in which the substituent does not interfere, may be used. Examples of suitable protecting groups and methods for their addition and removal may be found in the textbook "Protective Groups in Organic Synthesis" by T.W. Green, John Wiley and Sons, 1981. For example suitable protecting groups for amines are formyl or acetyl.
The following synthetic examples were prepared using the general preparative procedures described above:

Example 1: Benzyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate

a) Tetrahydro-2H-4-pyranyl trifluoromethanesulfonate. Pyridine (1.7 ml, 20.97 mmol) was added to a solution of tetrahydro-2H-4-pyranol (2 ml, 20.97 mmol) in dichloromethane (16 ml). The flask was immersed in an ice water bath and trifluoromethanesulfonic anhydride (3.6 ml, 20.97 mmol) in dichloromethane (7 ml) was added dropwise over 10 minutes. After 20 minutes, the reaction mixture was filtered and the solid was washed with minimum amount of
dichloromethane. The combined filtrate was washed with water, 1.0 N HCI, water and brine. The organic layer was dried (MgSO4) and filtered. The solvent was evaporated to give tetrahydro-2H-4-pyranyl trifluoromethanesulfonate. 'H NMR (CDCI3) δ 1.99 (m, 2H), 2.11 (m, 2H), 3.58 (m, 2H), 3.96 (m, 2H), 5.17 (m, IH).

b) 4-chloro-5-iodo-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidine. 4- Chloro-5-iodo-7H-pynolo[2,3-d]pyrimidine (3.0 g, 10.73 mmol) was added in small portions to a solution of sodium hydride ( 0.891g 22.2 mmol) in N,N- dimethylformamide (40 ml) at 0°C. After completed the addition the ice water bath was removed and the resulting mixture was stined for 30 minutes.
Tetrahydro-2H-4-pyranyl trifluoromethanesulfonate was added dropwise and the reaction mixture was stined at ambient temperature for 24 hours. The mixture was poured to ice water (lOOml) and the solid was collected by filtration and purified by re-crystallization to give 4-chloro-5-iodo-7-tetrahydro-2H-4-pyranyl- 7H-pynolo[2,3-d]pyrimidine. Η NMR (CDC13) δ 2.06 (m, 2H), 3.63 (m, 2H), 4.16 ( , 2H), 5.00 (m, IH), 7.45 (s, IH), 8.61 (s, IH). LC/MS (MH+=364)

c) tert-Butyl N-(4-(4-chloro-7-tetrahydro-2H-4-ρyranyl-7H-ρynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate. tert-Butyl N-[2-methoxy-4- (4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl]carbamate (1.66g, 4.75 mmol) in water was degassed by sonication under vacuum for 1 minute. 4- Chloro-5-iodo-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidine (1.1 g, 3.17 mmol), tetrakis(triphenylphosphine) palladium(O) (0.22 g, 0.19 mmol), Sodium carbonate (0.8g, 7.60 mmol) and 1,2-dimethoxyethane (30 ml) was added to the aqueous mixture. The resulting suspension was degassed again for 2 minutes and then headed to 85°C for 24 hours. The reaction mixture was cooled to ambient temperature and solvent was evaporated. The residue was dissolved in ethyl acetate. The organic layer washed and dried (MgSO4). The solid was purified by flash column chromatography on silica using heptane/ethyl acetate (7:3) as the mobile phase to give tert-butyl N-(4-(4-chloro-7-tetrahydro- 2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl) carbamate.

Η NMR (CDCI3) δ 1.55 (s, 9H), 2.10 (m, 4H), 3.66 (m, 2H), 3.92 (s, 3H), 4.16 (m, 2H), 5.05 (m, IH), 7.06 (m, 2H), 7.14 (s, IH), 7.32 (s, IH), 8.13 (br.d, J=8 Hz, IH), 8.64(s, IH). LC/MS (MH+=459)

d) 4-(4-chloro-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2- methoxyaniline. A solution often percent trifluoroacetic acid in
dichloromethane (50 ml) was added to tert-butyl N-(4-(4-chloro-7-tetrahydro- 2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate at 0°C. After 20 minutes, the ice water bath was removed and the resulting solution was stined at ambient temperature for 4 hours. The solvent was removed and the residue taken into dichloromethane. Saturated sodium bicarbonate was added and the layers separated. The aqueous layer was extracted with dichloromethane. The combined organic layer was washed with brine, dried (MgSO4), filtered and concentrated. The solid was purified by passing though a pat of silica gel using heptane/ethyl acetate (3 :2) as the mobile phase to give 4- (4-chloro-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2- methoxyaniline. Η NMR (CDC13) δ 2.09 (m, 4H), 2.51 (br. s, NH2), 3.66 (m, 2H), 3.91 (s, 3H), 4.16 (m, 2H), 5.05 (m, IH), 6.79 (d, J=8 Hz, 2H), 6.93 (d, J=8 Hz, IH), 6.98 (s, IH), 7.28 (s, IH), 8.63 (s, IH). LC/MS (MH+=359)

e) 5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-4-amine. Ammonium hydroxide (25 ml) was added to a solution of 4-(4-chloro-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2- methoxyaniline (0.73g, 2.03 mmol) in dioxane (25 ml) in a pressure tube. The pressure tube was sealed and heated to 122°C for 2 days. The tube was cooled to ambient temperature and the solvent was evaporated. Ethyl acetate was added and the organic layer was washed, dried (MgSO4), filtered and concentrated to give 5-(4-amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-4-amine. Η NMR (DMSO-d6) δ 1.87 (m, 2H), 2.11 (m, 2H), 3.52 (m, 2H), 3.79 (s, 3H), 3.99 (m, 2H), 4.87 (m, 3H), 6.02 (br. s, NH2), 6.73 (d, J=8

Hz, 2H), 6.77 (d, J=8 Hz, IH), 6.88 (s, IH), 7.33 (s, IH), 8.10 (s, IH). LC/MS

f) Benzyl N-(4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrirnidin- 5-yl)-2-methoxyphenyl)carbamate. Benzylchloroformate(16 uL, 0.110 mmol) was added dropwise to a stirring solution of 5-(4-amino-3-methoxyphenyl)-7- tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidm-4-amine (25 mg, 0.074 mmol) in pyridine (0.7 ml) and dichloromethane (0.7 ml) under nitrogen at 0°C. After 10 minutes, the ice water bath was removed and the resulting mixture was stined for 4 hours. The solvent was evaporated and the residue was purified by preparative TLC using dichloromethane/methanol (95:5) as the mobile phase to give benzyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl) carbamate. lH NMR (CDC13) δ 2.07 (m, 4H), 3.65 (m, 2H), 3.9 (s, 3H), 4.13 (m, 2H), 4.97 (m, IH), 5.23 (s, 2H), 6.96 (s, IH), 7.03 (s, IH), 7.08 (d, J=8 Hz, IH), 7.42 (m, 6H), 8.20 (br. s, J=8 Hz, IH).

8.32 (s, IH). LC/MS (MH"=474).

Example 2: Neopentyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate.
Neopentylchloroformate(13 uL, 0.110 mmol) was added dropwise to a stirring solution of 5-(4-amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4-amine (25 mg, 0.074 mmol) in pyridine (0.7 ml) and dichloromethane (0.7 ml) under nitrogen at 0°C. After 10 minutes, the ice water bath was removed and the resulting mixture was stined for 4 hours. The solvent was evaporated and the residue was purified by preparative TLC using
dichloromethane/methanol (95:5) as the mobile phase to give neopentyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyρhenyl)carbamate. Η MR (CDC13) δ 1.00 (s, 3H), 2.07 (m, 4H), 3.65 (m, 2H), 3.91 (s, 2H), 3.94 (s, 3H), 4.13 (m, 2H), 4.97 (m, IH), 5.18 (s, 2H), 6.97 (s, IH), 7.03 (s, IH), 7.07 (d, J=8 Hz, IH), 7.25 (s, IH), 8.19 (br. s, J=8 Hz, IH). 8.33 (s, IH). LC/MS (MH+=454).

Example 3: Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4-amine (100 mg, 0.294 mmol) was dissolved in dichloromethane (2 mL). Pyridine (2mL) was added followed by phenylchloroformate (44 uL, 0.353 mmol). After stirring for 3 hours, another 44 uL of phenylmethanesulfonyl chloride was added and the reaction mixture was stined overnight. The solvent was removed and the residue was purified by preparative LC/MS to give phenyl N-[4-(4-amino-7- tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (52 mg, 0.113 mmol). IH NMR (CDCl3-d) δ 2.09 (m, 4H), 3.66 (m, 2H), 3.98 (s, 3H), 4.16 (m, 2H), 4.98 (m, IH), 5.24 (s, 2H), 7.09 (m ,3H), 7.23 (m, 4H), 7.41 (m, 2H), 7.62 (s, IH), 8.20(bd, J=7.80 Hz, IH), 8.33 (s, IH). LC/MS MH+=460.

Example 4: Tetrahydro-2H-4-pyranyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate
Tetrahydro-2H-4-pyranol (1.0 ml, 10.5 mmol) was mixed with 4-methylmorpholine (2.0 ml) in dichloromethane (20 mL). 4- Nifrochloroformate (1.98 g, 9.82 mmol) was added slowly to the reaction mixture. After stirring for 5 hours, the reaction mixture was diluted with dichloromethane. The organic layer was washed with water, 1.0 N HCI, saturated sodium bicarbonate, brine, dried over MgSO4, filtered and evaporated. The crude product was purified by flash column chromatography chromatography using ethyl acetate/heptane (4: 1) as the mobile phase to give 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate (1.5 g, 5.62 mmol). IH NMR (CDCl3-d) δ 1.87 (m, 2H), 2.06 (m, 2H), 3.58 (m, 2H), 3.98 (m, 2H), 4.97 (m,lH), 7.40(d, J=9.0Hz, 2H), 8.30 (d, J=9.0Hz, 2H).

a) Tetrahydro-2H-4-pyranyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H- pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate. 5-(4-Amino-3- methoxyphenyl)-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4- amine (57 mg, 0.168 mmol) and 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate (90 mg, 0.336 mmol) was mixed in pyridine (1 mL). After stirring for 5 hours, another 90 mg of 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate was added and the reaction mixture was stined for 2 days. The reaction mixture was heated at 70°C for 2 hours. The solvent was removed and the residue was purified by preparative thin layer chromatography to give tetrahydro-2H-4- pyranyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrirm'din- 5-yl)-2-methoxyρhenyl]carbamate (30 mg, 0.064 mmol). IH NMR (CDCl3-d) δ 1.78 (m, 4H), 2.08 (m, 4H), 3.60 (m, 4H), 3.94 (s, 3H), 3.97 (m, 2H), 4.15 (m, 2H), 4.98 (m, 2H), 5.23 (s, 2H), 6.78 (s ,1H), 7.04 (s, IH), 7.07 (d, J=8.3 Hz, IH), 8.16(bd, J=7.90 Hz, IH), 8.33 (s, IH). LC/MS MH+=468.

Example 5 : 3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride

a) 4-Nitrophenyl (3-pyridylmethyl) carbonate. 4- Nifrochloroformate (2.49 g, 12.3 mmol) in dichloromethane (20 mL) was cooled on an ice-water bath. 3- pyridylmethanol (1.0 mL, 10.3 mmol) and 4-methylmorpholine (2.0 mL, 18.5 mmol) was added slowly. After 20 minutes, the ice-water bath was removed and the reaction mixture was allowed to warm up to room temperature. 30 minues later, ethyl acetate was added and the reaction mixture was filtered. The filtrate was washed with water, saturated sodium bicarbonate, brine, dried over MgSO4, filtered and evaporated to give a dark brown solid which was re-crystallized with ethyl acetate/heptane to give 4-nitrophenyl (3-pyridylmethyl) carbonate (1.52 g, 5.54 mmol).lH NMR (CDCl-d) δ 7.38 (m, 3H), 7.79 (m, IH), 8.28 (d,
J=9.09Hz, 2H), 8.65 (m, IH), 8.72 (s,lH).

b) 3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate. 5-(4-Amino-3-methoxyphenyl)- 7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4-amine (25 mg, 0.074 mmol) was dissolved in dichloromethane (0.7 mL). Pyridine (0.7 mL) was added followed by 4-nitrophenyl (3-pyridylmethyl) carbonate (30 mg, 0.110 mmol). After heating at 100°C overnight, the solvent was removed and the residue was purified by preparative LC/MS to give 3-pyridylmethyl N-[4-(4- ammo-7-tefrahydro-2H-4-pyranyl-7H-ρynolo[2,3-d]pyrimidin-5-yl)-2- methoxyphenyl]carbamate (12 mg, 0.025 mmol). IH NMR (CDCl3-d) δ 2.08 (m, 4H), 3.65 (m, 2H), 3.92 (s, 3H), 4.15 (m, 2H), 4.96 (m, IH), 5.26 (s, 2H), 5.54 (bs, 2H), 6.97 (s, IH), 7.04(s, IH), 7.08 (d, J=8.2Hz, IH), 7.35 (m, 2H), 7.79 (d, J=7.8Hz, IH), 8.15 (m, IH), 8.29 (s, IH), 8.61 (s, IH), 8.71 (s, IH). LC/MS MH 475

c) 3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride. 3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]ρyrimidin-5-yl)-2- methoxyphenyl]carbamate (12 mg, 0.025 mmol) was dissolved in ethyl acetate (2.0mL). 1.0N HCI in ether (1 mL) was added slowly. The precipatate was collected through filtration under nitrogen to give 3-pyridylmethyl N-[4-(4- amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2- methoxyphenyljcarbamate hydrochloride(13 mg, 0.25 mmol). IH NMR
(DMSO-d6) δ 1.91 (m, 2H), 2.17(m, 2H), 3.54 (m, 2H), 3.87 (s, 3H), 4.03 (m, 2H), 4.97(m, IH), 5.23 (s, 2H), 7.05 (d, J=8.2Hz, IH), 7.13 (s, IH), 7.51 ( , IH), 7.81 (d, J=8.2Hz, IH), 7.84 (s, IH), 7.95 (m, IH), 8.42 (s, IH), 8.60(s, IH),

8.71 (s, IH), 8.82 (s, IH). LC/MS MH 475.

Example 6: 2-Morpholinoethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (25 mg, 0.054 mmol) was mixed with 2-morpholino-l -ethanol (0.1 mL) in pyridine (0.7 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase HPLC to give 2-morpholinoethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (24 mg, 0.048mmol). The solid was dissolved in ethyl acetate (2 mL) and 1.0N HCI in ether (0.2 mL) was added slowly. The precipitate was collected through filtration under nitrogen to give 2-morpholinoethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyri_midin-5-yl)-2-methoxyphenyl] carbamate hydrochloride (24 mg, 0.045 mmol). IH NMR (DMSO- d6) δ 1.88(m, 2H), 2.16(m, 2H), 3.55 (m, 8H), 3.90 (s, 3H), 4.03 (m, 4H), 4.49(m, 2H), 4.92 (m, IH), 7.07 (m, IH), 7.15 (s, IH), 7.65 (bs, 2H), 7. 84 (s, IH), 8.45 (s, IH), 8.75(s, IH) 10.95 (bs, IH). LC/MS MH+=497.

Example 7 (4-Bromo-l ,3 -thiazol-5-yl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate.

a) 2,4-Dibromo-l,3-thiazole-5-carbaldehyde. l,3-Thiazolane-2,4-dione (3.52 g, 30 mmol) and phosphorus oxybromide (43 g, 150 mmol) were mixed with dimethyl formamide (2.56 mL, 34 mmol). The mixture was then heated at 75°C for 1 hours and at lOOoC for 5 hours. After cooled to room temperature, the mixture was added to ice-water (500ml) and the aqueous layer was extracted with dichloromethane. The combined organic layer was washed with saturated sodium bicarbonate, dried over MgSO4, filtered and evaporated to give a brown solid which was washed with petroleum ether. Evaporation of solvent gave 2,4- dibromo-l,3-thiazole-5-carbaldehyde (1.74 g, 6.42 mmol). IH NMR (CDCl3-d) δ 9.90 (S, IH).

b) (2,4-Dibromo-l,3-thiazol-5-yl)methanol. 2,4-Dibromo-l,3-thiazole-5- carbaldehyde (1.74 g, 6.42 mmol) was dissolved in methanol (70 ml) at 0°C. Sodium borohydride (0.244 g, 6.42 mmol) was added in small portions. The ice- water bath was removed 10 minutes later and the reaction mixture was stined at room temperature overnight. Solvent was removed and saturated ammonium chloride was added. 1.ON NaOH was added to adjust the pH to 10. The aqueous layer was extracted with ethyl acetate. The combined organic layer was washed with brine, dried over MgSO4, filtered and evaporated. The residue was purified by flash column chromatogrphy to give (2,4-dibromo-l,3-thiazol-5-yl)methanol (0.946 g, 3.47 mmol). IH NMR (CDCl3-d) δ 2.11 (bs, IH) δ 4.79 (S, 2H).

c) (4-Bromo-l,3-thiazol-5-yl)methanol. (2,4-Dibromo-l,3-thiazol-5~yl)methanol (0.94 g, 3.44 mmol), sodium carbonate xri-hydrade (1.34 g) and palladium on carbon (10%, 0.07g) were mixed in methanol (33 mL). The resulting mixture was hydrogenated at 60 psi for 2 days. The solid was filtered off through a pat of celite. The solvent was evaporated and the residue was purified by frash column chromatography to give (4-bromo-l,3-thiazol-5-yl)methanol (0.32 g, 2.78 mmol). IH NMR (CDCl3-d) δ 2.29 (bs, IH) δ 4.86 (s, 2H), 8.72 (s, IH).

d) (4-Bromo- 1 ,3-thiazol-5-yl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl- 7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate. Phenyl N-[4-(4- amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2- methoxyphenyl]carbamate (28 mg, 0.061 mmol) was mixed with (4-bromo-l,3- thiazol-5-yl)methanol (50 mg, 0.434 mmol) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give (4-bromo-l ,3- thiazol-5-yl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]ρyrimidin-5-yl)-2-methoxyρhenyl]carbamate. IH NMR (CDCl-d) δ 2.07(m, 4H), 3.65 (m, 2H), 3.92 (s, 3H), 4.13 (m, 2H), 4.98 (m, IH), 5.35 (s, IH), 5.40(s, 2H), 6.97 (s, IH), 7.04 (s, IH), 7.09 (m, IH), 7.35 (s, IH), 8.17 (s, IH), 8.32 (s, IH), 8.78(s, IH). LC/MS MH+=481.

Example 8: Tefrahydro-3-furanyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-ρynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed with tefrahydro-3-furanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase PHLC to give tefrahydro-3-furanyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (14 mg, 0.031mmol). IH NMR (CDCl-d) δ 2.07(m, 6H), 3.66 (m, 2H), 3.96 (m, 7H), 4.13 (m, 2H), 4.98 (m, IH), 5.26 (s, 2H), 5.40(m, IH), 6.97 (s, IH), 7.04 (s, IH), 7.08 (d, J=8.2Hz, IH), 7.26 (s, IH), 8.30 (s, IH), 8.32 (s, IH). LC/MS MH+=455.

Examples 9 and 10: l,3-Dioxan-5-yl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
1 ,3 -Dioxolan-4-ylmethyl N-(4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H~ pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed glycerol formal (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase PHLC to give tetrahydro-3-furanyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (2 mg, 0.004mmol). IH NMR (CDCl-d) δ 2.06(m, 4H), 3.66 (m, 2H), 3.92 (m, 3H), 4.07 (m, 6H), 4.79 (m, IH), 4.83 (d, J=6.3Hz, IH), 4.96 (m, IH), 5.04(d, J-6.3HZ, IH), 6.15 (vbs, 2H), 6.96 (s, IH), 7.05 (m, 2H), 7.53 (s, IH), 8.15 (d, J=8.2Hz, IH), 8.22 (s, IH). LC/MS
and l,3-dioxolan-4-ylmethyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl) carbamate(6.0mg, 0.013 mmol). IH NMR (CDCl-d) δ 2.06(m,

4H), 3.66 (m, 2H), 3.75 (m, IH), 3.92 (m, 3H), 4.03 (m, IH), 4.13 (m, IH), 4.34 (m, 2H), 4.94 (s, IH), 4.97 (m, IH), 5.10(s, IH), 5.32 (bs, 2H), 6.97 (s, IH), 7.03 (m, 2H), 7.06 (d, J=8.2Hz, IH), 7.38(s, IH), 8.15 (d, J=7.9Hz, IH), 8.31 (s, IH). LC/MS MH 71.

Example 11 : 2-Pyridyhnethyl N-[4-(4-aιnino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrirnidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride
Phenyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed 2-pyridylmethanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC MS to give 2-pyridylmethyl N-[4-(4-amino-7-tefrahydro-2H-4-ρyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (11 mg, 0.023 mmol). The solid was dissolved in ethyl acetate (2 mL) and l.ON HCI in ether (0.1 mL) was added slowly. The precipitate was collected through filtration under nitrogen to give 2-pyridylmethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate
hydrochloride (12 mg, 0.023 mmol). IH NMR (DMSO-d6) δ 1.92(m, 2H), 2.16(m, 2H), 3.55 (m, 2H), 3.89 (s, 3H), 4.02 (m, 2H), 4.91 (m, IH), 5.23 (s, 2H), 7.05 (d, J-8.2Hz, IH), 7.14 (s, IH), 7.37 (m, IH), 7. 53 (d, J=7.8Hz, IH), 7.87 (m, 3H), 8.42(s, IH), 8.57 (d, J=4.2Hz, IH), 8.85 (s, IH). LC/MS MH+=475.

Example 12: 4-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate Hydrochloride
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed 4-pyridylmethanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give 2-pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl] carbamate (11 mg, 0.023 mmol). The solid was dissolved in ethyl acetate (2 mL) and l.ON HCI in ether (0.1 mL) was added slowly. The precipatate was collected through filtration under nitrogen to give 4-pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2 -methoxyphenyl] carbamate
hydrochloride (12 mg, 0.023 mmol). IH NMR (DMSO-d6) δ 1.91(m, 2H), 2.16(m, 2H), 3.55 (m, 2H), 3.90 (s, 3H), 4.03 (m, 2H), 4.92 (m, IH), 5.34 (s, 2H), 7.06 (d, J=8.2Hz, IH), 7.16 (s, IH), 7.73 (m, IH), 7. 81 (m, IH), 7.87 (s, IH), 8.46(s, IH), 8.76 (d, J=5.6Hz, IH), 9.05 (s, IH). LC/MS: MH+=475.

Example 13 : (5-Methyl-3-isoxazolyl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-ρynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed with (5-methyl-3-isoxazolyl)methanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give (5-methyl-3-isoxazolyl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-' d]pyrimidin-5 -yl)-2-methoxyphenyl] carbamate (18 mg, 0.038mmol) . 1 H NMR (CDCl-d) δ 2.06(m, 4H), 2.44 (s, 3H), 3.64 (m, 2H), 3.91 (s, 3H), 4.13 (m, 2H), 4.96 (m, IH), 5.26 (s, 2H), 6.12(s, IH), 6.95 (s, IH), 7.06 (m, 2H), 7.39 (s, IH), 8.17 (bs, IH), 8.21(s, IH). LC/MS: MH" 479.

Example 14: [(2S)-5-Oxotefrahydro-lH-2-pynolyl]methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed with (5S)-5-(hydroxymethyl)tetrahydro-lH-2-pynolone (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give [(2S)-5-oxotefrahydro-lH-2-pynolyl]methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (10 mg, 0.021mmol).

IH NMR (CDCl-d) δ 1.90 (m, IH), 2.06(m, 4H), 2.34 (m, IH), 2.41 (m, 2H), 3.64 (m, 2H), 3.94 (s, 3H), 4.04(m, 2H), 4.14 (m, 2H), 4.98 (m, IH), 5.33 (m, 3H),

6.10(s, IH), 6.98 (s, IH), 7.04 (s, IH), 7.09 (m, IH), 7.31(s, IH), 8.11 (bs, IH), 8.32

(s, IH). LC/MS: MH+ 481.

Example 15: 4-Ammobeι__^l N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo [2,3 -d]pyrimidin-5 -yl)-2-methoxyphenyl)carbamate a) tert-Butyl N-(4-(hydroxymethyl)phenyl)carbamate. (4-Aminophenyl)methanol (1.23 g, 10 mmol) and diisopropylethylamine ( 2.6 mL, 15 mmol) was mixed with di-tert-butyl dicarbonate (2.62 g, 12 mmol) in dichloromethane (50 mL). The mixture was stined at room temperature overnight. Ethyl acetate was added and the organic layer was washed with water, 1.ON HCI, saturated sodium carbonate, water, brine, dried over MgSO4, filtered and evaporated. The crude product was purified by flash column chromatography with Ethyl acetate/ heptane (2:3) to give tert-butyl N-(4-(hydroxymethyl)phenyl) carbamate (2.16g, 9.67 mmol). IH NMR (CDCl-d) δ 1.52 (s, 9H), 4.63 (s, 2H), 6.47 (bs, IH), 7.30

(d, 8.5Hz, 2H), 7.36 (d, 8.5Hz,2H).

b) 4-Aminobenzyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate. Phenyl N-[4-(4-amino-7- tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2- methoxyphenyl]carbamate (51mg, 0.111 mmol) was mixed with tert-butyl N-(4- (hydroxymethyl)phenyl)carbamate (119 mg, 0.533) in pyridine (0.8 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give 4- aminobenzyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl)carbamate (9 mg, 0.015mmol). IH NMR (CDCl-d) δ 1.52(s, IH), 2.08(m, 4H), 3.65 (m, 2H), 3.90 (s, 3H), 4.14(m, 2H), 4.97 (m, IH), 5.17 (s, 2H), 5.37(bs, IH), 6.55 (s, IH), 6.95 (s, IH), 7.03 (s, IH), 7.06 (m, IH), 7.31(s, IH), 7.38 (m, 3H), 8.16 (bs, IH), 8.30 (s, IH). LC/MS: MH+ 589.

Example 16: Nl-[4-(4-Amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]benzamide
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4-amine (80mg, 0.236 mmol) was dissolved in dichloromethane (2.0 mL). Pyridine (2.0 mL) was added followed by benzoyl chloride (41 uL, 0.353 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give Nl-[4-(4- amino-7-tetrahydro-2H-4-pyranyl-7H-pyτrolo[2,3-d]pyrimidin-5-yl)-2- methoxyphenyl]benzamide (64 mg, 0.144 mmol). IH NMR (CDCl3-d) δ 2.12 (m, 4H), 3.67 (m, 2H), 3.99 (s, 3H), 4.17(m, 2H), 4.99 (m, IH), 7.03(s, IH), 7.04 (s, IH), 7.14 (d, J=8.2Hz, IH), 7.53 (m, 3H), 7.94(d, J=7.8Hz, IH), 8.33 (s, IH), 8.58 (s, IH), 8.63 (d, J=8.2Hz, IH). LC/MS: MlT=444

Example 17: N2-[4-(4-Amino-7-tefrahydro-2H-4-pyranyl-7H-ρynolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl]-2-pyridinecarboxamide
5-(4-Amino-3-methoxyphenyl)-7-tefrahydro-2H-4-ρyranyl-7H-pynolo[2,3- d]pyrimidin-4-amine (80mg, 0.236 mmol) was dissolved in dichloromethane (2.0 mL). Pyridine (2.0 mL) was added followed by 2-pyridinecarbonyl chloride hydrochloride (63 mg, 0.353 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1

mL) was added and precipitate was formed. The solid was collected by filtration to give Nl-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)- 2-methoxyphenyl]benzamide (84 mg, 0.189 mmol). IH NMR (CDCl3-d) δ 2.12 (m, 4H), 3.67 (m, 2H), 4.03 (s, 3H), 4.14(m, 2H), 5.00 (m, IH), 5.37 (s, IH), 7.04(s, IH), 7.09 (s, IH), 7.14 (d, J=8.2Hz, IH), 7.50 (m, IH), 7.92 (m, IH), 8.33 (s, IH), 8.70(d, J=8.2Hz, IH), 10.62 (s, IH). LC/MS: MH+=445.

Example 18: N5-[4-(4-Amino-7-tefrahydro-2H-4-ρyranyl-7H-ρyπolo[2,3- d]pyrimidin-5-yl)-2-methoxyphenyl]-l,3~dimethyl-lH-5-pyrazolecarboxamide
5 -(4- Amino-3 -methoxypheny l)-7~tetrahydro-2H-4-pyranyl-7H-pynolo [2,3 - d]pyrimidin-4-amine (80mg, 0.236 mmol) was dissolved in dichloromethane (2.0 mL). Pyridine (2.0 mL) was added followed by 2-pyridinecarbonyl chloride hydrochloride (63 mg, 0.353 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give N5-[4-(4-amino-7-tefrahyά^o-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-memoxyphenyl]-l,3-dimethyl-lH-5-pyrazolecarboxamide (30 mg, 0.065 mmol). IH NMR (CDCl3-d) δ 2.11 (m, 4H), 2.32 (s, 3H), 3.66 (m, 2H), 3.99 (s, 3H), 4.13(m, 2H), 4.17 (s, 3H), 4.99 (m, IH), 5.22 (bs, 2H), 6.46 (s, IH), 7.03 (s, IH), 7.07 (s, IH), 7.12 (d, J=8.2Hz, IH), 8.33 (2, 2H), 8.49(d, J=8.2Hz, IH). LC/MS: MH+=462.

Example 19: Nl-[4-(4-Amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylpropanamide
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-ρynolo[2,3-d]pyrimidin-4-amine (50mg, 0.147 mmol) was dissolved in dichloromethane (1.5 mL). Pyridine (1.5 mL) was added followed by 2,2-dimethylpropanoyl chloride (31 mg, 0.221 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give Nl-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylproρanamide (27 mg, 0.064 mmol). IH NMR (CDC13-d) δ 1.35 (s, 9H), 2.09 (m, 4H), 3.66 (m, 2H), 3.96 (s, 3H), 4.13(m, 2H), 4.97 (m, IH), 5.46(bs, 2H), 6.98 (s, IH), 7.04 (s, IH), 7.07 (d, J=8.2Hz, IH), 8.15 (s, IH), 8.29 (s, IH), 8.49(d, J=8.2Hz, IH). LC/MS: MH+=424.

Example 20: Nl-[4-(4-Amino-7-tetrahydro-2H-4-ρyranyl-7H-ρynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]- 1 -cyclopentanecarboxamide
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4-amine (50mg, 0.147 mmol) was dissolved in dichloromethane (1.5 mL). Pyridine (1.5 mL) was added followed by 1-cyclopentanecarbonyl chloride (31 mg, 0.221 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give Nl-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylpropanamide (33 mg, 0.076mmol). IH NMR (CDC13-d) δ 1.66 (m, 2H), 1.81 (m, 2H), 1.95 (m, 4H), 2.06 (m, 4H), 2.77 (m, IH), 3.65 (m, 2H), 3.94 (s, 3H), 4.15(m, 2H), 4.96 (m, IH), 5.37(bs, 2H), 6.98 (s, IH), 7.03 (s, IH), 7.07 (d, J=8.2Hz, IH), 7.84 (s, IH), 8.30 (s, IH), 8.49(d, J=8.2Hz, IH).
LC/MS: MH+=437.

Example 21 : Nl -[4-(4-Amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]-3-phenylpropanamide
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-4-amine (50mg, 0.147 mmol) was dissolved in dichloromethane (1.5 mL). Pyridine (1.5 mL) was added followed by 3-phenylpropanoyl chloride (37 mg, 0.221 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give Nl-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylpropanamide (7 mg, 0.015mmol). IH NMR (CDCl3-d) δ 2.07 (m, 4H), 2.75 (m, 2H), 3.09 (m,2H), 3.65 (m, 2H), 3.88 (s, 3H), 4.13(m, 2H), 4.96 (m, IH), 5.97(bs, 2H), 6.93 (s, IH), 7.05 (m, 2H), 7.26 (m, 5H), 7.70 (s, IH), 8.24 (s, IH), 8.46(d, J=8.2Hz, IH). LC MS: MH+=472.

Example 22: 5-(4-phenoxyphenyl)-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine.

a) Tosyl chloride (12.0 g) was added in portions to a mixture of 3- hydroxytetrahydofuran (5.0 g) in pyridine (100 ml) at 0 C under nitrogen with stirring. The mixture was sti ed at 0°C for 2 hours and then warmed to ambient temperature. The mixture was stined at ambient temperature for 72 hours. The mixture was cooled to 0°C and 5M hydrochloric acid (200 ml) was added. The mixture was extracted with ethyl acetate and the combined ethyl acetate extracts were washed with 2M hydrochloric acid and then with brine, then dried, filtered and evaporated to give 3-tosyloxytetrahydrofuran as an oil.

b) Sodium hydride (120 mg, of a 60% dispersion in mineral oil) was added to a solution of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine
(906 mg) and dimethylformamide (30 ml) with stirring under nitrogen. The mixture was stined for 30 minutes and then a solution of 3-(tosyloxy) tefrahydrofuran (750 mg) in dimethyl formamide (10 ml) was added with stirring. The mixture was stined and heated at 95°C for 18 hours and then evaporated under vacuum. The residue was partitioned between ethyl acetate and water. The ethyl acetate layer was separated, dried and evaporated to give a residual gummy solid which was triturated with ether and filtered to give 5-(4- phenoxyphenyl)-7-(3-tefrahydfofuryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine m.p. 196-196.5°C.

Example 23 : 5 -(4-phenoxyphenyl)-7-(4-tetrahydropyranyl)-7H-pynolo [2,3 -d]pyrimidin-4-ylamine.
In a similar manner to Example 1, 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine was reacted with 4-tosyloxytefrahydropyran to give after flash column chromatography 5-(4-phenoxyphenyl)-7-(4-tetrahydropyranyl)-7H-pyπolo[2,3-d]pyrimidin-4-ylamine, m.p. 193-193.5°C.

Example 24: 4-amino-5-(4-phenoxyphenyl)-7-[4-(N-tert-butoxycarbonyl) tetrahydroisoxazolyl]-7H-pynolo[2,3-d]pyrimidin-4-ylamine.

a) Di-tert-butyl dicarbonate (4.56 g) was added to a solution of 4- hydroxytetrahydroisoxazole (2.4 g) and triethylamine (4.2 g) in tetrahydrofuran (100 ml) with stirring at 0°C under nitrogen. The mixture was stined at ambient temperature for 72 hours and then filtered. The filtrate was evaporated under reduced pressure to give N-(tert-butoxycarbonyl)-4-hydroxytetrahydroisoxazole as an oil which was used directly in the next part of this example.

b) The product from a) above (3.6 g) was stined in pyridine (50 ml) at 0°C under nitrogen and then tosyl chloride (3.62 g) was added in portions at 0°C with stirring. The mixture was stined at 0°C for 1 hour and then allowed to warm to ambient temperature over 18 hours. The pyridine was removed under reduced pressure and ethyl acetate (50 ml) and citric acid (50 ml of a IM solution in water) were added. The organic layer was separated and washed with IM citric acid solution and then brine, then dried, filtered and evaporated to give an oil which was purified by flash column chromatography using petroleum ether, b.p 40-60°C containing 20-30% of ethyl acetate as the mobile phase.
Appropriate fractions were collected and combined to give N-(tert- butoxycarbonyl)-4-tosyloxy tefrahydroisoxazole, m.p. 63-65°C.

c) A solution of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (1.0 g) in dimethylformamide (40 ml) was added dropwise with stirring to a suspension of sodium hydride (0.145 g, of a 60% dispersion in mineral oil) in dimethylformamide (60 ml) with stirring under nitrogen at 0°C. The mixture was stined at 0°C for 1 hour and then the product from b) (1.25 g) was added. The mixture was heated at 100°C for 3 hours and then cooled to ambient temperature, quenched with water and extracted with ethyl acetate to give an oil. The oil was triturated with ethyl acetate and the solid obtained was collected by filtration to give 4-amino-5-(4-phenoxyphenyl)-7-[4-(N-tert- butoxycarbonyl)tefrahydroisoxazolyl]-7H-pynolo[2,3-d]pyrirmdm-4-yl_mιine, m.p. 162-163°C.

Example 25: 5-(4-phenoxyphenyl)-7-(4-tetrahydroisoxazolyl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine dihydrochloride.

The product from Example 3 (0.29 g) was dissolved in dichloromethane (8 ml) and then stined at 0°C whilst trifluoroacetic acid (2.0 ml) was added. The mixture was allowed to warm to ambient temperature and stined at ambient temperature for 2 hours. The mixture was basified with sodium bicarbonate solution and extracted with dichloromethane to give an oil which was purified by flash column chromatography using ethyl acetate and then ethyl acetate/methanol (9:1) as the mobile phase. The appropriate fractions were collected and combined, then evaporated to give a solid which was dissolved in ethyl acetate and then treated with ethereal hydrogen chloride (3.0 ml, of a IM solution). The solid obtained was collected by filfration, washed with ether and dried under vacuum at 45 °C for 2 hours to give 5-(4-phenoxyphenyl)-7-(4-tefrahydroisoxazolyl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine dihydrochloride, m.p. 208°C (with decomposition).

Example 26: 4-chloro-5-iodo-7-(3-tefrahydroft_ryl)-7H-pynolo[2,3-d]pyrimidine

a) 4-Chloro-5-iodo-7H-pynolo[2,3-d]pyrimidine (5.0 g) was added to a mixture of sodium hydride (0.79 g of a 60% dispersion in mineral oil) in
dimethylformamide (100 ml) with stirring under nitrogen at 0°C. The mixture was stined until hydrogen evolution ceased. 3-Tosyloxytetrahydrofuran (4.65 g) was added at 0°C and then the mixture was warmed to 90°C. The mixture was stined at this temperature for 2 hours and then overnight at ambient temperature. Water (100 ml) was added cautiously and the mixture was extracted with ethyl acetate to give 4-chloro-5-iodo-7-(3-tefrahyα_rofuryl)-7H-pynolo[2,3- d]ρyrimidine, m.p. 184-186°C.

b) A mixture of 4-iodophenol (25.0 g), 2-fluorobenzaldehyde (14.14 g), potassium carbonate (31.5 g) and dimethylformamide (500 ml) was heated at 120°C under nitrogen with stirring for 15 hours. The mixture was cooled to ambient temperature and filtered. Water (500 ml) was added to the filtrate and the mixture was extracted with ethyl acetate to give a solid which was triturated with hot hexane (500 ml). The supernatant liquid was decanted from a residual gum and cooled. The solid which precipitated was collected by filtration to give 2-(4-iodophenoxy) benzaldehyde, m.p. 84.5-86°C.

c) Toluene (250 ml) was deoxygenated and then nitrogenated for 30 minutes. 2-(4- Iodophenoxy)benzaldehyde (6.46 g), hexamethylditin (10.0 g) and tetrakis (triphenylphosphine) palladium (0) (1.4 g) were added to the toluene. The mixture was boiled under reflux under nitrogen with stirring for 7 hours. The mixture was cooled to ambient temperature then filtered. The filtrate was evaporated and the residue was purified by flash column chromatography on silica using 3% ethyl acetate in petroleum ether, b.p. 40-60°C as the mobile phase to give 2-(4-trimethylstannylphenoxy)benzaldehyde as an oil.

d) A mixture of the product from c) (1.80 g), the product from b) (1.76 g), tris(dibenzylideneacetone)dipalladium (228 mg), triphenylarsine (383 mg) and dimethylformamide (75 ml) was heated at 65°C under nitrogen with stirring for 70 hours. The mixture was cooled to ambient temperature and quenched with water. The mixture was extracted with ethyl acetate to give a residue which was purified by flash column chromatography on silica using increasing amounts of ethyl acetate from 30-50% in petroleum ether, b.p. 40-60°C as the mobile phase to give a solid which was triturated with diethyl ether and filtered to give 2-[(4- (4-chloro-7-(3-tetrahydrofuryl)-7H-pynolo[2,3-d]ρyrimidin-5- yl)phenoxy]benzaldehyde as a solid.

e) The product from d) (360 mg) was dissolved in methanol (5 ml) and sodium borohydride (65 mg) was added at 0°C with stirring. The mixture was warmed to ambient temperature and stined at this temperature for 1 hour. The mixture was quenched with dilute sodium hydroxide solution and then evaporated under reduced pressure to give a residue which was extracted with ethyl acetate to give 2-[(4-(4-chloro-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyι_imidin-5- ylphenoxyjbenzyl alcohol.

f) A mixture ofthe product from e) (280 mg), 1,4-dioxane (15 ml) and
concentrated aqueous ammonia solution (15 ml, S.G. 0.88) was heated at 120°C in a pressure vessel for 20 hours. The mixture was cooled to ambient temperature and the solvent removed under reduced pressure. The residue was- taken up in ethyl acetate, washed with water, then dried, filtered and evaporated to give an oil which was purified by flash column chromatography on silica using ethyl acetate/methanol (9:1) as the mobile phase to give 2-[(4-(4-amino-7- (3-tefrahydroftιryl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]benzyl alcohol as a glassy solid, m.p. 92-96°C.

Example 27: 2-[4-(4-amino-7-(3-tefrahydroft_ryl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-N,N-diethylbenzylamine

a) Sodium triacetoxyborohydride (264 mg) was added to a mixture of 2-[(4- (4-chloro-7-(3 -tetrahydrofuryl)-7H-pynolo [2,3 -d]pyrimidin-5 - yl)phenoxy]benzaldehyde (330 mg) and diethylamine (121 mg) in 1,2- dichloroethane in a vial (5 ml) and the vial septum sealed. The mixture was stined at ambient temperature for 20 hours then quenched with saturated aqueous sodium bicarbonate solution (5 ml). The mixture was extracted with ethyl acetate to give 2-[4-(4-chloro-7-(3-tefrahydrofuryl)-7H-pynolo[2,3- d)pyrimidin-5-yl)phenoxy]-N,N-diethylber zylamine.

b) A mixture ofthe product from a) (280 mg), concentrated aqueous ammonia solution (10 ml, S.G. 0.88) and 1,4-dioxane (10 ml) was heated in a pressure vessel for 16 hours at 120°C. The mixture was cooled and the solvent removed under reduced pressure. The residue was taken up in ethyl acetate, washed with water, then dried, filtered and evaporated to give an oil which was purified by flash column chromatography using ethyl acetate/methanol as a mobile phase to give 2-[4-(4-ammo-7-(3-tefrahydrofttryl)-7H-pynolo[2,3-d]pyrimidin-5- yl)ρhenoxy]-N,N-diethylbenzylamine, m.p. 107-110°C.

Example 28: 2-[4-(4-armno-7-(3-tefrahydroflιιyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-benzonitrile

a) A mixture of 2-fluorobenzonitrile (28.8 g), 4-bromophenol (36.9 g), potassium carbonate (58.9 g) and dimethylformamide (30 ml) was heated with stirring under nitrogen at 120°C for 5 hours. The mixture was allowed to stand overnight at ambient temperature and then partitioned between ethyl acetate and water. The organic layer was separated, washed, dried and evaporated to give an oil which solidified on standing. The solid was triturated with petroleum ether b.p. 40-60°C and filtered to give 2-(4-bromophenoxy)benzonitrile.

b) A mixture from the product of part a) (5.57 g), hexamethylditin (10.0 g), tefrakis (triphenylphosphine) palladium (0) (1.4 g) and degassed toluene (250 ml) was heated at 110°C with stirring under nitrogen for 4.5 hours. The mixture was allowed to stand for 18 hours at ambient temperature and then filtered through a silica pad. The pad was washed with ethyl acetate and the combined filtrate and washes evaporated to dryness. The residue was purified by flash column chromatography on silica using petroleum ether b.p. 40-60°C and diethyl ether (2%) increasing to 5% as the mobile phase. Appropriate fractions were collected combined and evaporated to give 2-(4-trimethylstannylphenoxy)benzonixrile.

c) A mixture 4-chloro-5-iodo-7-(3-tefrahycfro_nιyl)pynolo[2,3-d]ρyrimidihe (1.8 g, prepared as described in Example 5) and the product from part b) (1.23 g) were reacted and then worked up in a similar manner to Example 5d) to give 2-[4-(4- chloro-7-(3-tefrahy(fro_hryl)-7H-pynolo[2,3-d]pyrirnidin-5- yl)phenoxy]benzonitrile.

d) A mixture ofthe product from c) (470 mg), concenfrated aqueous ammonia (33 ml, SG 0.880) and 1,4-dioxane (33 ml) were heated together in a pressure vessel at 120°C for 18 hours and then worked up on a similar manner to Example 5 to give 2-[4-(4-aιnmo-7-(3-tefrahydrofiιryl)-7H-pynolo[2,3-d]ρyrimidin-5- yl)phenoxy]-benzonitrile, m.p. 201-203°C.

Example 29: 2-[4-(4-amino-7-(3-tefrahydroftιryl)-7H-ρynolo[2,3-d)pyrimidin-5-yl)phenoxy]benzaldehyde

a) In a similar manner to Example 2, 3-tosyloxytetrahydrofuran (1.84 g) was reacted with 5-(4-benzyloxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (2.9 g) using sodium hydride (0.30 g, of a 60% dispersion in mineral oil) and dimethylformamide (40 ml) except that the mixture was heated for 4.5 hours at 90°C to give 5-(4-beι__2yloxyphenyl)-7-(3-tefrahydrofuryl)-7H-pynolo[2,3- d]pyrimidin-4-ylamine as a solid.

b) A mixture ofthe product from part a) (6.0 g), 10% palladium on charcoal (3.0 g), ammonium formate (4.9 g) and ethanol (500 ml) was heated on a steam bath with stirring under nitrogen for 2 hours. The mixture was cooled and filtered and the solvent evaporated. The filtrate was concentrated to half volume and filtered to give a solid which was identified as 4-[4-amino-7-(3-tefrahydrofiιryl)- 7H-ρynolo[2,3-d]pyrimidin-5-yl]phenol m.p. 257-259°C.

c) A mixture of 4-[4-amino-7-(3-tefrahyάϊofuryl-7H-pynolo[2,3-d]pyrimidin-5- yl]phenol (2.55 g), 2-fluorobenzaldehyde (1.07 g), potassium carbonate (2.13 g) and dimethylformamide (80 ml) was heated at 120°C with stirring under nitrogen for 5 hours. The mixture was cooled to ambient temperature quenched with water and extracted with ethyl acetate to give 2-[4-(4-amino-7-(3- tefrahy(frofuιyl)-7H-pynolo[2,3-d)pyrimidin-5-yl)phenoxy]benzaldehyde, m.p. 185-187°C.

Example 30: 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo-[2,3-d]pyrimidin-7-yl]tetrahydrofuran-3-ol
Sodium hydride (120 mg of a 60% dispersion in mineral oil) was added to a solution of 4-ammo-5-(4-phenoxyphenyl-7H-pynolo[2,3-d]pyιimidine (902 mg) and dimethylformamide (30 ml) with stirring under nitrogen. The mixture was stined for 30 minutes and then 3,6-dioxabicyclo[3.1.0]hexane (300 mg) was added and the mixture was warmed to 80°C. The mixture was left for 64 hours and then evaporated under reduced pressure. The residue was triturated with water which left an oily gum. Ether was added and the mixture was stined rapidly for 30 minutes which gave a solid which was collected by filtration and washed with methanol. The solid was discarded. The filtrate produced a second crop of solid which was recrystallised from ethanol to give 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo-[2,3-d]pyrimidin-7-yl]tefrahydrofuran-3-ol, m.p. 234.5-235.5°C.

Example 31: 5-[4-(2-moιpholinomethylphenoxy)phenyl]-7-(3-tefrahydrofiιryl)-7H-pynolo [2 ,3 -d]pyrimidin-4-y lamine
A mixture of 2-[4-(4-ammo-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d)pyrimidin-5-yl)phenoxy]benzaldehyde (0.15 g), morpholine (64 mg), sodium triacetoxyborohydride (117 mg) and 1,2 dichloroethane (5 ml) was stined at ambient temperature for 18 hours. Saturated aqueous sodium bicarbonate solution was added and the mixture was filtered through an EMPORE® cartridge. The filtrate was evaporated and the residue was dissolved in dichloromethane (5 ml) and then tris(2-aminoethyl)amine-polymer bound (0.3 g) and 2 drops of glacial acetic acid were added and the mixture was stined at ambient temperature overnight. The polymer was removed by filfration and washed with dichloromethane and then with methanol. The combined organic filtrate and washings were evaporated under reduced pressure to give an oil which was triturated with diethyl ether/ethyl acetate with warming to dissolve the solid and then the solution was cooled in ice and filtered to give 5-[4-(2-morpholmomethylphenoxy)phenyl]-7-(3-tefrahydrofuryl)-7H-ρynolo[2,3-d]pyrimidin-4-ylamine, m.p. 169-171°C.

Example 32: 5-[4-(2-piperidinomemylphenoxy)phenyl]-7-(3-tetrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
In a similar manner to Example 10, 2-[4-(4-an_ no-7-(3-tefr_ιhydrofuryl)-7H-pynolo[2,3-d)pyrimidin-5-yl)phenoxy]benzaldehyde (0.15 g) was reacted with piperidine (63 mg) to give 5-[4-(2-piperidinomethylphenoxy)phenyl]-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine m.p. 76-78°C (glassy foam).

Example 33: 5-{4-[2-(2-methoxyethyl)aminomethylphenoxy]phenyl}-7-(3-tetrahydrofiιι l)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine
In a similar manner to Example 10, 2-[4-(4-amino-7-(3-tefr_ώ.ydrofuryl)-7H-pyπolo[2,3-d)pyrimidin-5-yl)phenoxy]benzaldehyde (0.15 g) and 2-methoxyethylamine (56 mg) were reacted together to give 5-{4-[2-(2-methoxyethyl)aminomethylphenoxy]phenyl} -7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine m.p. 66-68°C (glassy foam).

Example 34: 4-[(4-(4-amino-7-(3-tefrahyαϊo_uι l)-7H-pynolo[2,3-d]-pyrimidin-5-yl)phenoxy]benzyl alcohol

a) In a similar manner to Example 9, 4-[4-amino-7-(3-tefrahydrofuryl)-7H- pynolo[2,3-d]pyrimidin-5-yl]phenol was reacted with 4-fluorobenzaldehyde to give 4-[4-(4-amino-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-5- yl)phenoxy] benzaldehyde.

b) The product from a) (0.35 g) was dissolved in methanol (10 ml) and to this solution was added sodium borohydride (32 mg) at 0°C. The mixture was warmed at ambient temperature and stined at this temperature for 10 minutes. 1,2-Dichloroethane (4 ml) was added to aid solubility. The mixture was stined to ambient temperature for 18 hours and then glacial acetic acid (1 ml) was added and the mixture evaporated under reduced pressure. The residue was partitioned between ethyl acetate and saturated aqueous sodium carbonate solution. The ethyl acetate was separated, dried, filtered and evaporated to give 4-[(4-(4-ammo-7-(3-tefrahydrofui l)-7H-pynolo[2,3-d]-pyrimidin-5- yl)phenoxy]benzyl alcohol, m.p. 92-95°C.

Example 35: 5-[4-(4-fluorophenoxy)phenyl]-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
A mixture of 4-[4-ammo-7-(3-tefrahydrofuryl-7H-pynolo[2,3-d]pyrimidin-5-yl]phenol (0.59 g), 4-fluorophenylboronic acid (0.56 g), copper (II) acetate (0.36 g), triethylamine (1.01 g), dichloromethane (20 ml) and activated ground 4 molecular sieves (0.5 g) was stined under nitrogen in a dry atmosphere for 64 hours. The reaction mixture was filtered through a small pre-flushed silica pad and eluted with dichloromethane (200 ml) then ethyl acetate (250 ml) and finally ethyl
acetate/methanol 9:1 (250 ml). The dichlormethane and ethyl acetate fractions were combined and purified by flash column chromatography on silica using ethyl acetate/methanol as the mobile phase to give 5-[4-(4-fluorophenoxy)phenyl]-7-(3-tetrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine, m.p. 198-199°C.

Example 36: 5-[4-(4-morpholinomethylphenoxy)-ρhenyl]-7-(3-tefrahydroftιryl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
In a similar manner to Example 10 a mixture of 4-[4-(4-amino-7-(3-tefrahydroftιryl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]benzaldehyde (336 mg), and morpholine (146 mg) were reacted to give 5-[4-(4-morpholinomethylphenoxy)-phenyl]-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidm-4-yl__mine, m.p 142-144°C.

Example 37: 5-[4-(3-morpholinomemylphenoxy)phenyl]-7-(3-tefrahydrofuryl)-7H-ρynolo[2,3-d]pyrimidin-4-ylamine

a) A mixture of 4-[4-amino-7-(3-tefrahydro_nr l-7H-pynolo[2,3-d]pyrimidin-5- yl)phenol (0.297 g), was reacted with 3-formylphenylboronic acid in a similar manner to Example 14 to give 3-[4-(4-amino-7-(3-tefrahy< oftιryl)-7H- pynolo[2,3-d]pyrimidin-5-yl)phenoxy]benzaldehyde.

b) The product from part a) (100 mg) and morpholine (44 mg) were reacted together using similar reagents and conditions as described in Example 10 to give 5- [4-(3 -morpholinomethylphenoxy)phenyl] -7-(3 -tetrahydrofuryl)-7H- pynolo[2,3-d]pyrimidin-4-ylamine, m.p. 83-85°C.

Example 38: 2-[4-(4-amino-7-(3-tefrahydroftιryl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(2-(4-pyridyl)ethylamino)-benzonitrile
A mixture of 4-[4-amino-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-phenol (0.517 g), 2-fluoro-6-(2-(4-pyridinyl)ethylamino)benzonitrile (0.42 g), potassium carbonate (0.48 g) and dimethylformamide (20 ml) were heated at 120°C under nitrogen for 8 hours. The mixture was allowed to cool, diluted with water then extracted with ethyl acetate to give a solid which was recrystallised from ethyl acetate to give solid which was purified by flash column chromatography on silica using ethyl acetate and then ethyl acetate/methanol (9:1, 8:1, 4:1) to give 2-[4-(4-amino-7-(3-tefrahydrofuryl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(2-(4-pyridyl) ethylamino)-benzonitrile, m.p 212-213°C.

Example 39: 2-[4-(4-amino-7-(3-tefrahydroftιryl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(3-imidazol-l-yl)propylaminobenzonixrile
4-[4-Ammo-7-(3-tefrahydro_uryl)-7H-pynolo[2,3-d]pyrimidin-5-ylphenol (0.49 g), 2-fluoro-6-(3-iιrndazol-l-yl)propylamino benzonitrile, potassium carbonate (0.45 g) and dimethylformamide were reacted in a similar manner to Example 17 to give 2-[4-(4-ammo-7-(3-tefrahydro_^l)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(3-imidazol-l-yl)propylaminobenzonitrile, m.p.H0°C (glassy foam).

Example 40: 4-amino-6-bromo-5-(4-phenoxyphenyl)-7-(3-tefrahyά_rofuryl)-7H-pynolo[2,3-d]pyrimidine

a) A mixture of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (302 mg) was dissolved in dimethylacetamide (10 ml) and dichloromethane (50 ml) and then treated with N-bromosuccinimide (178 mg) in dichloromethane (10 ml). The mixture was left stirring ambient temperature for 16 hours. The mixture was evaporated under reduced pressure and the residue was triturated with water to give a solid which was collected by filtration and dried to give 4- amino-6-bromo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine, m.p. 282- 283°C.

b) A mixture ofthe product from a) (1.14 g) in dry dimethylformamide (30 ml) was stined under nitrogen whilst sodium hydride (120 mg of a 60% dispersion in mineral oil) was added. This was followed by 3-tosyloxytetrahydrofuran (0.8 g) in dimethylformamide (10 ml). The mixture was heated at 90°C overnight. The mixture was evaporated under reduced pressure and the residue was triturated with water to give a solid which was collected by filtration and dried to give a solid which was purified by dissolving in ethanol, adding water to cloud point and filtering. The filtrate was evaporated under reduced pressure to give a residue which was purified by flash column chromatography on silica to give 4- amino-6-bromo-5-(4-phenoxyphenyl)-7-(3-tefrahydrofuryl)-7H-pynolo[2,3- d]pyrimidine, m.p. 205-206°C.

Example 41: 2-[4-(4-aιnino-7-(3-tefrahydro_nιyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(3-methoxypropylamino) benzonitrile In a similar manner to Example 17, 4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidine (0.65 g), 2-fluoro-6-(3-methoxypropylamino)benzonitrile (0.46 g), potassium carbonate (0.61 g) and dimethylformamide (40 ml) was heated under nitrogen at 120°C for 8 hours to give, after workup, 2-[4-(4-amino-7-(3-tefrahydroftuyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(3-memoxypropylamino) benzonitrile, m.p.l83-184°C.
Example 42 : 2-[4-(4-amino-7-(4-tetrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]benzonitrile

a) A mixture of 5-(4-benzyloxyphenyl)-7-(tefrahydropyran-4-yl)-7H-pvnolo[2,3- d]pyrimidin-4-ylamine (2.83 g), 10% palladium on carbon (1.41 g), ammonium formate (2.31 g) and ethanol (250 ml) was boiled under reflux under nitrogen with stirring for 1.5 hours. The mixture was cooled to ambient temperature, filtered, then the filtrate cooled and filtered. The filtrate was evaporated to give a solid 4-[4-amino-7-(4-tetrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5- yl]phenol.

b) A warm solution of 4-[4-amino-7-(4-tetrahydropyranyl)-7H-pynolo[2,3- d]pyrimidin-5-yl]phenol (0.082 g) in dimethylformamide (3.4 ml) was added to a mixture of 2-fluorobenzonitrile (80 mg) and potassium carbonate (76 mg) in a vial. The vial was flushed with nitrogen then sealed. The mixture was shaken at 120°C for 6 hours and then left to cool to ambient temperature over 16 hours. The mixture was diluted with water (11 ml) and then extracted with ethyl acetate to give 2-[4-(4-amino-7-(4-tefrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5- yl)phenoxy]benzonitrile, m.p. 125°C (softens).

Examples 43-48 were prepared in a similar manner to the previous example by reacting 4-[4-amino-7-(4-tefrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]phenol with the appropriate nitrile except that the mixtures were shaken together for periods up to 48 hours. The reactions were monitored for the disappearance of starting material and heated for the appropriate time.

Example 49: 2-[4-(4-Amino-7-(4-tetrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(3-imidazol-l-yl)propylaminobenzonitrile from 2-fluoro-6-(3-(imidazol- 1 -yl)propyl_ιmino)-benzonitrile.

Example 50 : 2-(4-(4-Amino-7-(4-tefrahydropyranyl)-7H-pynolo [2,3 -d]pyrimidin-5-yl)phenoxy]- 6-(2-morpholinoethoxy)benzonitrile, m.p. 110°C (glass), from 2-fluorobenzonitrile.

Example 51 : 2-[4-(4-Amino-7-(4-tetrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-6-(2-(4-pyridyl)ethylamino)benzonitrile, m.p. 120-123°C (glass), from 2-fluoro-6-(2-(4-pyridyl)ethylamino)benzonitrile.

Example 52 : 2- [4-(4- Amino-7-(4-tetrahydropyranyl)-7H-pynolo [2,3 -d]pyrimidin-5-yl)phenoxy)-6-(3-methoxypropylamino)benzonitrile, m.p. 205-207°C, from 2-fluoro-6-(3-methoxy-propylamino)benzonitrile.

Example 53: 2-[4-(4-Amino-7-(4-tetrahydropyranyl)-7H-pynolo[2,3-d]pyrimidin-5-yl)phenoxy]-5-fluorobenzonitrile, m.p. 216-217°C, from 2,5-difluorobenzonitrile.

Examples 54-101

General Method
Portions ofthe amines listed in Table 1 (9 molar equivalents with respect to the ester employed, weights ranging from 47.5 mg to 184.5 mg) were weighed into separate vials and methanol (1 ml) was added to each vial. A solution of ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl acetate (1 molar equivalent) in a mixture of methanol and triemylamine (4 ml, ratio of methanol to Iriethylamine is 23.2:1 v/v. The reaction mixtures were shaken at 60-65° C for 36 hours. The methanol and triethylamine were removed under reduced pressure at 50°C for 3 hours and to each vial was added water (3 ml) followed by
dichloromethane (3 ml). The vials were agitated for 15 seconds and then allowed to stand for 18 hours. The mixtures were poured into EMPORE®(10 mm/6 ml) extraction disk cartridges and the dichloromethane phases were collected and evaporated at 50°C for 3 hours. During work-up it was observed that solid had separated out in the vials on standing for 18 hours. Consequently the aqueous layer in each cartridge was forced through with compressed air. Dichloromethane (4 ml) was added to each extraction cartridge. Each filtrate was evaporated under reduced pressure at 50°C for 3 hours. The desired products were either found in the original dichloromethane extract, in which case they are indicated as being present in the liquid, or were found in the insoluble solid on reworking and are refened to as being found in the solid. Certain products were found in both phases. These phases are indicated in Table 1.
Each sample was analysed by LCMS and in each case the target ion was found. The retention time for each product is given in Table 1. The conditions used are given below.

Column: 5 μm hypersil BDS c18 (100 x 2.1 mm).
Mobile Phase: 0.1M NH40Ac [pH 4.55] : MeCN (gradient - see below).

Conditions: 10-100% MeCN in 8 minutes.
(Gradient) 100% MeCN for 1 minute.
100-10% MeCN in 2 minutes.
(Total analysis run time 11 minutes.
Flow Rate: 1 ml/minute (no split in MS).
Wavelength Range: 250-320 nm
Injection Volume: 20 μl.
MS
Method: APCI11H.
Ionisation APcI +ve/-ve.
Mass Range: 100-700 m/z.
Cone voltage: 20.

In a similar manner to Examples 54-101, the amines listed in Table 2 were reacted, respectively, with ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-7-yl]propionate to give the products listed in Examples 102-146 respectively. The work-up and the analysis conditions were identical to those used for Examples 54-101. In each case the target ion was found by LCMS.

TABLE 1

The compounds prepared are given below.

Example 54: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-hydroxyethyl)acetamide

Example 55 : 4-Amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-7-yl-N-(l-hydroxyprop-2-yl)acetamide

Example 56: 4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-hydroxypropyl)acetamide Example 57: 4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyriιmdin-7-yl-N-(2-methoxyethyl)acetamide

Example 58 : 4-An_ no-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(3-hydroxypropyl)acetamide

Example 59: (S)-4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-( 1 -hydroxyprop-2-yl)acetamide

Example 60: (R )-4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-hydroxypropyl)acetamide

Example 61 : 4-Aniino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(N,N-dimethylamino)ethyl]acetamide

Example 62: 4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(1 -hydroxybut-2-yl)acetamide

Example 63 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-hydroxybutyl)acetamide

Example 64: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2 ,3 -dihy droxypropyl)acetamide

Example 65: (S)-4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2,3-dihydroxypropyl)acetamide

Example 66: (R)-4-AnΗno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrintidin-7-yl-N-(2,3-dihydroxypropyl)acetamide Example 67: 4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]ρyrimidin-7-yl-N,N-(3-azapentamethylene)acetamide

Example 68: 4-An___no-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3 -(N,N-dimethylamino)propyl] acetamide

Example 69: 4-An___no-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[ 1 -CNf,N-dimethylamino)prop-2-yl]acetamide

Example 70: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(N,N-dimethylamino)propyl]acetamide

Example 71 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-( 1 -hydroxy-3 -methylbut-2-yl)acetamide

Example 72: 7-{2-[4-(2-Morpholino-2-oxoethyl)piperazin-l-yl]-2-oxo-ethyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine

Example 73 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(l-hydroxy-3-methylprop-2-yl)acetamide

Example 74: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(l,3-dihydroxy-2-methylprop-2-yl)acetamide

Example 75: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(2-hydroxyethoxy)ethyl]acetamide

Example 76: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(pynolidin- 1 -yl)ethyl]acetamide Exa ple 77: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N,N-(3-azahexamethylene)acetamide

Example 78: 4-An_dno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyriniidin-7-yl-N-[ 1 -(hydroxymethyl)cyclopentyl] acetamide

Example 79: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-hydroxycyclohexyl)acetamide

Example 80: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(N,N-diethylamino)ethyl] acetamide

Example 81 : 4-Amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(3-hydroxypropylamino)ethyl]acetamide

Example 82: 4-Amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(2-hydroxyethylthio)ethyl]acetamide

Example 83 : 4-Amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(pyrid-2-yl)ethyl]acetamide

Example 84: 4-Amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2-(pyrid-3-yl)ethyl]acetamide

Example 85: 4-_ mino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3 -(imidazol- 1 -yl)propyl] acetamide

Example 86: 7-{2-[4-(2-Morpholinoethyl)piperazin-l-yl]-2-oxo-ethyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrh din-4-ylamine Example 87: 4-Aιr__no-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(N-ethylpynolidin-2-yl)methylacetamide

Example 88: 4-An__ino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-piperidinoethyl)acetamide

Example 89: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3-(pynolidin-l-yl)propyl]acetamide

Example 90: (R)-4-Amino-5-(4-phenoxvphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(N-ethylpynolidin-2-yl)methylacetamide

Example 91 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(2-morpholinoethyl)acetamide

Example 92: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3-(N,N-diethylamino)propyl]acetamide

Example 93 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3-(N,N-dimethylamino)-2,2-dimethylpropyl]acetamide

Example 94: 7-[2-(4-Ethoxycarbonylpiperazin-l-yl)-2-oxoethyl]-5-(4-phenoxyphenyl)-7H-pynolo [2,3 -d]pyrimidin-4-ylamine

Example 95: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[2,2-bis(hydroxymethyl)butyl]acetamide

Example 96: 4-Annno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3 -(2-pynolidinon- 1 -yl)propyl] acetamide Example 97: 4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(3-piperidinoρropyl)acetamide

Example 98 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(3-morpholinopropyl)acetamide

Example 99: 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-(3 -hydroxy- 1 -methylprop-2-yl)acetamide

Example 100: 4-An_ino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[3-(N-3-aminopropyl,N-methyl)aminopropyl]acetamide

Example 101 : 4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl-N-[N-bis(2-aminoethyl)aminoethyl]acetamide

TABLE 2

Example 102: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-hydroxyethyl)propanamide

Example 103: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-( 1 -hydroxyρrop-2-yl)propanamide

Example 104: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-hydroxypropyl)propanamide

Example 105: l-[4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-methoxyethyl)propanamide

Example 106: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(3 -hydroxypropyl)proρanatnide

Example 107: (S)-l-[4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(l-hydroxyprop-2-yl)propanamide Example 108: (R)-l-[4-Ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-hydroxypropyl)propanamide

Example 109: l-[4-An__ino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrirnidin-7-yl]-N-[2-(N,N-dimethylamino)ethyl]propanamide

Example 110: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(l -hydroxybut-2-yl)propanamide

Example 111: 1 -[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-hydroxybutyl)propanamide

Example 112: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2,3-dihydroxypropyl)propanamide

Example 113: (S)-l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2,3-dihydroxypropyl)propanamide

Example 114: (R)-l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2,3-dihydroxyproρyl)propanamide

Example 115: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[3-(N,N-dimethylamino)propyl]propanamide

Example 116: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2-(N,N-dimethylamino)propyl]propanarnide

Example 117: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N- [ 1 -(N,N-dimethylamino)prop-2-yl]propanamide Example 118: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-( 1 -hydroxy-3 -methylbut-2-yl)propanamide

Example 119: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyιirnidin-7-yl]-N-[2-(2-hydroxyethylamino)ethyl]propanamide

Example 120: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-( 1 -hydroxy-2-methylprop-2-yl)propanamide

Example 121: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(l,3-dihydroxy-2-methylprop-2-yl)propanamide

Example 122: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N~[2-(2-hydroxyethoxy)ethyl]propanamide

Example 123: 1 -[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyιimidin-7-yl]-N-[2-(pynolidin-l-yl)ethyl]propanamide

Example 124: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[ 1 -(hydroxymethyl)cyclopentyl]propanamide

Example 125 : l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-hydroxycyclohexyl)propanamide

Example 126: l-[4-An_ano-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimi(_in-7-yl]-N-[2-(N,N-diethylamino)ethyl]propanamide

Example 127: l-[4-Anfrno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2-(3-hydroxypropylaιnino)ethyl]propanamide Example 128: 1 -[4-Anaino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2-(2-hydroxyethylthio)ethyl]propanamide

Example 129: l-[4-An_ no-5-(4-phenox} henyl)-7H-ρynolo[2,3-d]pyrirnidin-7-yl]-N-[2-(pyrid-2-yl)ethyl]propanamide

Example 130: 1 -[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2-(pyrid-3-yl)ethyl]propanamide

Example 131: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[3-(imidazol-l-yl)propyl]propanamide

Example 132: 1 -[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2-(N-methylpynolidin-2-yl)ethyl]propanamide

Example 133: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[(N-ethylpynolidin-2-yl)methyl]propanamide

Example 134: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-piperidinoethyl)propanamide

Example 135: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[3-(pynolidin-l-yl)propyl]propanamide

Example 136: (R)-l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[(N-ethylpynolidin-2-yl)methyl]propanamide

Example 137: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(2-morpholinoethyl)propanamide Example 138: 1 -[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[3-(N,N-diethylamino)propyl]propanamide

Example 139: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[3-(N,N-dimethylamino)-2,2-dimethylpropyl]propanamide

Example 140: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2,2-bis(hydroxymethyl)butyl]propanamide

Example 141: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[3-(2-pynolidinon-l-yl)propyl]propanamide

Example 142: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(3 -piperidinopropyl)propanamide

Example 143: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-(3 -morpholinopropyl)propanamide

Example 144: 1 -[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-[2-(N,N-di-isopropylamino)ethyl]propanamide

Example 145: 1 - [ Amino-5 -(4-phenoxyphenyl)-7H-pynolo [2,3 -d]pyrimidin-7-yl] -N-[3-(N-3-aminopropyl,N-methyl)aminopropyl]propanamide

Example 146: l-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-N-P r-bis(2-aminoethyl)aminoethyl]propanamide

Example 147 : 2- [4-amino-5 -(4-phenoxyphenyl)-7H-pynolo [2,3 -d]pyrirnidin-7-yl]--buxyrolactone
a) 4-Ammo-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (1.0 g) was added to a mixture of sodium hydride (0.158 g of a 60% dispersion in mineral oil) in dimethyl formamide (70 ml) with stirring under nitrogen at 0°C. The mixture was stined at 0°C for 1 hour and then α-bromo-γ-buxyrolactone (0.60 g) in dimethylformamide (6 ml) was added dropwise with stirring at 0°C. The mixture was stined at ambient temperature for 18 hours and then quenched with water

(100 ml). The mixture was extracted with ethyl acetate. The combined extracts were dried and evaporated to give 2-[4-amino-5-(4-phenoxyphenyl)-7H- pynolo[2,3-d]pyrimidin-7-yl]~butyrolactone as an oil which was used directly in b).

b) N,N-Dimethylethylenediamine (5.0 ml) was added to a mixture ofthe product from a) (1.2 g) and pyridin-2-one (50 mg) in toluene (100 ml). The mixture was heated to 100°C for 2 hours and then evaporated to dryness under reduced pressure. The residue was suspended in ethyl acetate and washed with water. The organic extracts were then extracted with 5M hydrochloric acid (3 x 50 ml) and the acidic extracts were washed with ethyl acetate then basified with 6M sodium hydroxide solution at 0°C and then back extracted with ethyl acetate and then dichloromethane. The combined organic extracts were dried, filtered and evaporated to give an oil which was crystallised from ethyl acetate/ether to give 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-4-hydroxy- N-[2-dimethylamino)ethyl]utyramide, m.p. 178-179°C.

Example 148: Ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]propionate
Sodium hydride (120 mg, of a 60% dispersion in mineral oil) was added to a mixture of 4-__mino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (906 mg) in dry dimethyformamide (30 ml) and the mixture was stined under nitrogen for 30 minutes at ambient temperature. A solution of ethyl 2-bromopropionate (543 mg) in dry DMF (10 ml) was added dropwise via a syringe over 10 minutes. The mixture was stined at ambient temperature for 2 hours and then left for 18 hours. The mixture was evaporated under vacuum and the residue was washed with water to give a solid which was triturated with ether and filtered to give ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo [2,3 -d]pyrimidin-7-yl]propionate, m.p . 139- 140°C .

Example 149: N-(2-dimethylaminoethyl)-2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)propionamide
A mixture of ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-7-yl]propionate (425 mg), N,N-dimethylethylenediamine (2 ml) and methanol (20 ml) was boiled under reflux for 18 hours with the exclusion of carbon dioxide. The mixture was cooled and filtered, the filtrate was diluted with water (50 ml) and stined with ether. The mixture was left standing for 18 hours and the solid which precipitated was collected by filtration, washed with water and then ether and dried to give N-(2-dimethylaminoethyl)-2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)propionamide, m.p. 163-164°C.

Example 150: Ethyl 2-[4-amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]acetate
A mixture of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (906 mg), sodium hydride (120 mg, of a 60% dispersion in mineral oil) and dry dimethylformamide (30 ml) was stined at ambient temperature under nitrogen for 30 minutes. Ethyl bromoacetate (0.5 g) in dimethylformamide (10 ml) was added over 5 minutes at 0-5°C with stirring. The mixture was stined for 30 minutes at ambient temperature and then allowed to stand for 18 hours. The mixture was evaporated under vacuum and the residue was triturated with water and ether. The solid obtained was collected by filtration, washed with water and then with ether to give ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]acetate, m.p. 161-161.3°C.

Examples 151-156
General Method
Ethyl 2-[4-armno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl] acetate (194 mg) was heated at 62°C and stined with 10 molar equivalents ofthe appropriate amine as listed below in methanol (12 ml) for 18 hours to give after work up the following compounds:

Example 151
N-[2-hydroxyethyl-l,l-di(hydroxymethyl)]-2-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo [2,3 -d]pyrimidin-7-yl] acetamide, m.p. 222-223°C with decomposition, from 2-hydroxyethyl-l,l-di(hydroxymethyl)ethylamine.

Example 152
N-[2-(piperazin-l-yl)ethyl]-2-[4-amino-5-[4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-7-yl]acetamide, m.p.l38-140°C, from 2-(piperazin-l-yl)ethylamine.

Example 153
N-(2-morpholinoethyl)-2- [4-amino-5 -(4-phenoxyphenyl)-7H-pyπolo [2,3 -d]pyrimidin-7-yl] acetamide, m.p. 164-165°C, from 2-morpholinoethylamine.

Example 154
N-[3 -( 1 -imidazol)propyl] -2-[4-amino-5 -(4-phenoxyphenyl)-7H-pynolo [2,3 -d]pyrimidin-7-yl] acetamide, m.p. 170-171°C, from 3-(l-imidazolyl)propylamine.

Example 155
N-(N-ethylpynolidin-2-ylmemyl)-2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-7-yl]acetamide, m.p. 122-122.5°C, from 1-(N-ethylpynolodin-2-yl)methyl-amine.

Example 156
N-[-2(2-hydroxyethoxy)ethyl]-2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-7-yl]acetamide, m.p. 145-147°C, from 2-(2-hydroxyethoxy)ethylamine.

Example 157: 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yljpropionic acid
A mixture of ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidin-7-yl]propionate (201 mg), aqueous potassium hydroxide solution (4 ml of 2M solution) and methanol (20 ml) was boiled under reflux for 1 hour. The mixture was concentrated under reduced pressure to around 5 ml and then diluted with water (30 ml). The mixture was hot filtered and filtrate was cooled and then acidified with dilute acetic acid until no further precipitation occuned. The mixture was heated on a hot plate until the gel which had been obtained became a finely divided solid. The solid was collected by filtration to give 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]propionic acid, m.p. 239.5-241°C.

Example 158: Ethyl 4-[4-amido-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]butyrate
A mixture of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine

(1.5 g) was dissolved in DMF (30 ml) and treated with sodium hydride (0.22 g of a 60% dispersion in mineral oil) and then with ethyl 4-bromobutyrate (1.08 g) in DMF (15 ml) in a similar manner to Example 95 to give ethyl 4-[4-amido-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]butyrate, m.p. 104-104.5°C.

Example 159: ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]carbox-amide
In a similar manner to Example 97, 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-4-yl amine (1.0 g), sodium hydride (1.032 g of a 60% dispersion in mineral oil), 2-bromoacetamide (0.55 g) and dimethylformamide (50 ml) were reacted together to give after work-up a solid which was recrystallised from isopropanol to give ethyl 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]carbox-amide, m.p. 232-233°C.

Example 160: 2-[4-amino-5-(4-phenoxyphenyl)pynolo[2,3-d]pyrimidin-7-yl]-2-methylpropionamide
4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (200 mg) was dissolved in l,3-dimethyl-3,4,5,6-tefrahydro-2-(lH)-pyrimidinone (1.5 ml) with stirring and sodium hydroxide (0.158 g) was added at ambient temperature and the mixture stined for 15 minutes. 2-Bromo-2-methylpropanamide (0.5 g) was added and the mixture was stined vigorously for 18 hours at ambient temperature under a water-free atmosphere, then further 2-bromo-2-methylpropanamide (0.15 g) was added and stined for a further 24 hours. Water (3 ml) was added to the reaction mixture together with dilute hydrochloric acid (5M) to adjust the pH to 0. The suspension was added to water (60 ml) and the mixture left to stand for 18 hours at ambient temperature. The solid was collected by filtration, washed well with water and dried under high vacuum at 50°C. The solid was purified by preparative HPLC (reverse phase). Appropriate fractions were collected and combined and extracted with dichloromethane. Evaporation ofthe dichloromethane gave 2-[4-amino-5-(4-phenoxyphenyl)pynolo[2,3-d]pyrimidin-7-yl]-2-methylρropionamide, m.p. 227-228°C.

Example 161: 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin-7-yl] N-(2-dimethylaminoethyl)buxyramide A mixture of ethyl 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin-7-yl]butyrate (100 mg) in 30 ml methanol was heated under reflux with 0.6 ml 2-dimethylammoe ylamine for 18 hours. The mixture was evaporated under reduced pressure and the residue was heated with 2-dimethylaminoethylamine (10 ml) on a steam bath for 18 hours. Excess amine was removed under reduced pressure. Water was added to the residue and the mixture filtered to give 4-[4-__mino-5-(4-phenoxyphenyl)-7H-pyndlo[2,3-d]pyi__min-7-yl] N-(2-dimemyl_uninoethyl)butyramide.

Examples 162, 163 and 164 were prepared in a similar manner to Example 108 by reacting the same ester with the appropriate amine listed.

Example 165
4-[4-amino-5-(4-phenoxyphenyl)-7H-ρynolo[2,3-d]pyrimin-7-yl]-N-[3-(l-imidazolyl)propyl]butyramide from 3-(l -imidazolyl)propylamine.

Example 166
4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin-7-yl]-N-(2-mo holinoethyl)butyramide from 2-moφholinoethylamine.

Example 167
4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin-7-yl]-N-(3-morpholinopropyl)butyramide from 3-morpholinopropylamine.

Preparation of Starting Materials
a) Tert-butylamine (15 ml) was added with stirring to a solution of 2-bromo-4'- phenoxyacetophenone (12.7 g, prepared by bromination of 4'- phenoxyacetophenone according to Tetrahedron Letters, 1993, 34, 3177) in propan-2-ol and the mixture heated at 80°C for 3 hours. The mixture was cooled to 0°C and concenfrated hydrochloric acid (10 ml) added. The suspension was stined at ambient temperature for 18 hours and the solid collected by filfration to give 4'-phenoxy-2-(tert-butylamino)acetophenone hydrochloride (3.75 g), m.p. 210-212°C.

1) 4'-Phenoxy-2-(tert-butylamino)acetophenone hydrochloride (3.75 g) was added in one portion to sodium ethoxide (prepared by dissolving sodium
(93 mg) in ethanol (50 ml)) and the mixture was stined at 40°C for 30
minutes under nitrogen.

2) In a separate flask sodium (331 mg) was dissolved in ethanol (50 ml) and malononitrile (858 mg) was added. The solution was stined at ambient temperature for 5 minutes and then to this solution was added the solution of 4 '-phenoxy-2-(tert-butylamino)acetophenone obtained in part ( 1 ) in one portion excluding the precipitated sodium chloride. The resultant mixture was heated at 50°C for 3 hours and then at 80°C for 2 hours. The solvent was removed under reduced pressure and the resultant oil was partitioned
between water and ethyl acetate. The organic phase was separated, dried and evaporated to give a black solid. This solid was dissolved in hot ethanol and triturated with water, filtered and dried to give 2-amino-3-cyano-4-(4- phenoxyphenyl)- 1 -(tert-butyl)pynole.

b) A mixture of 2-amino-3 -cyano-4-(4-phenoxyphenyl)- 1 -(tert-butyl)pyπole (1.9 g), formamide (30 ml) and 4-dimethylaminopyridine (10 mg) was heated at

180°C for 6 hours. The mixture was cooled to ambient temperature and water was added to precipitate a dark solid. The solid was collected by filtration, washed with water, then boiled up in ethanol and the insoluble material collected by hot filtration and dried. The solid was purified by preparative HPLC on a silica column using dichloromethane/propan-2-ol/ethanol, 98:1:1 as the mobile phase to give 7-tert-butyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4- ylamme (4-ammo-5-(4-phenoxyphenyl)-7-(tert-butyl)pynolo[2,3-d]pyrimidine), m.p. 157-158°C. IH NMR (d6 DMSO) δ 8.15 (lH,s), 7.50-7.35 (4H,m), 7.30 (lH,s), 7.15 (lH,t), 7.10 (4H,m), 6.05 (2H,brs), 1.75 (9H,s).

c) A mixture of 4-amino-5-(4-phenoxyphenyl)-7-(tert-butyl)pynolo[2,3-d]- pyrimidine (5.8 g), glacial acetic acid (55 ml) and hydrobromic acid (55 ml of a 48%o solution) was boiled under reflux for 18 hours under nitrogen. The mixture was allowed to cool and a solid was collected by filfration. This solid was washed with methanol and then with ether to give 4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidine hydrobromide, m.p. 288-292°C.

The hydrobromide salt was converted into the free base by warming with dilute sodium hydroxide solution (100 ml of 5% w/v solution) and ethanol (60 ml) with stirring and removing the ethanol by distillation. The mixture was cooled and the solid was collected by filfration and washed well with water to give 5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine, m.p. 272°C.

Example 168: 7-cyclopentanesulphonyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrirnidin-4-ylamine
Sodium hydride (0.132 g of a 60% dispersion in mineral oil) was added to a solution of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (1.0 g) in dry dimethylformamide (30 ml) with stirring under nitrogen. The mixture was stined for 30 minutes and then cyclopentanesulphonyl chloride (0.558 g, prepared as described in J.O.C.1952, 17, 1529-1533) in dry dimethylformamide (5 ml) was added dropwise. The mixture was allowed to stand for 72 hours and then evaporated under vacuum. The residue was triturated with water and filtered to give a solid which was washed well with water, then stined with ethyl acetate then filtered. The filtrate was purified by flash column chromatography on silica using ethyl acetate as the mobile phase. Appropriate fractions were collected and evaporated to give 7-cyclopentanesulphonyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine, m.p. 188-188.5°C.

Example 169: 5-(4-phenoxyphenyl)-7-(8-phmalimidoocxyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
Sodium hydride (120 mg of a 60% dispersion in mineral oil) was added to a solution of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (906 mg) in dry dimethylformamide (30 ml) with stirring under nitrogen. The mixture was stined for 30 minutes under nitrogen and then N-(8-bromooctyl)phthalimide (1.4 g) in dimethyl-formamide (5 ml) was added. The mixture was stined at ambient temperature for 18 hours under nitrogen and then partitioned between water and ethyl acetate. The ethyl acetate layer was separated and purified by flash column chromatography using ethyl acetate as the mobile phase to give 5-(4-phenoxyphenyl)-7-(8-phthalinndooctyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine, m.p. 85-86°C.

Example 170: 7-(8-aminooctyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine dihydrochloride dihydrate
A mixture of 5-(4-phenoxyphenyl)-7-(8-phthalimidooctyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (1.0 g), hydrazine hydrate (1.0 ml) and ethanol (40 ml) was boiled under reflux for 2 hours with the exclusion of carbon dioxide. The mixture was cooled for 18 hours and a solid which precipitated was collected by filfration and discarded. The filtrate was evaporated under reduced pressure and the residue was dissolved in ethyl acetate, dried and then treated with a solution of concentrated hydrochloric acid in isopropanol dropwise until no further precipitation occuned. The mixture was left to stand overnight, then supernatent liquid was decanted off and the semi-solid residue was triturated with ethyl acetate to give 7-(8-aminooctyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine dihydrochloride dihydrate, m.p 120°C.

Example 171 : N-{2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl] ethyl} phthalimide In a similar manner to Example 468, but with additional heating at 90°C for 3 hours, 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine was reacted with 2-bromoethylphthalimide to give N-{2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]ethyl}phthalimide, m.p. 111-112°C.

Example 172: 7-(2-aminoethyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine hydrochloride
In a similar manner to Example 469, the product from the previous example was treated with hydrazine hydrate to give 7-(2-aminoethyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine hydrochloride, m.p. 284-285°C.

Example 173 : 7-isobuxyryl-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
Isobutyryl chloride (1.8 g) was added dropwise to a mixture of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (4.32 g), dry dimethyl-formamide (200 ml) and dry pyridine (2 ml) with stirring under nitrogen at 20°C. The mixture was stined at ambient temperature for 1 hour and evaporated under vacuum. The residue was partitioned between water and ethyl acetate. The ethyl acetate was separated, dried and evaporated and the residue obtained was recrystallised from toluene to give 7-isobutyryl-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine, m.p. 160.5-161°C.

Example 174: 5-(4-phenoxyphenyl)-7-(l ,4-dioxaspiro[4,5]decan-8-yl)-7H-pynolo [2,3 -d]pyrimidin-4-ylamine
Sodium hydride (0.26 g of a 60% dispersion in mineral oil) was added to a mixture of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (1.94 g) in dimethylformamide (50 ml) at ambient temperature with stirring. The mixture was stined until the evolution of hydrogen ceased and then 8-tosyloxy-l,4-dioxaspiro[4,5] decane (2.0 g, prepared as described in US 4,360,531 from 1,4-dioxaspiro[4,5]decan-8-one, (which was prepared according to J. Med. Chem. 1992, 2246)) was added. The mixture was heated at 120°C for 5 hours under nitrogen, cooled to ambient temperature, quenched with water and extracted with ethyl acetate to give a residue which was purified by flash column chromatography on silica using ethyl acetate followed by ethyl acetate containing increasing amounts of methanol up to 6% to give 5-(4-phenoxyphenyl)-7-(l,4-dioxaspiro[4,5]decan-8-yl)-7H-pyπolo[2,3-d]pyrimidin-4-ylamine, m.p. 193-194°C.

Example 175 : 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexanone
The product from the previous example (500 mg), acetone (20 ml) and 3M hydrochloric acid (10 ml) was stined under nitrogen at ambient temperature for 20 minutes. The mixture was then heated at 60°C for 1 hour and then the acetone was removed under reduced pressure. The residue was basified with aqueous 5M sodium hydroxide solution and then extracted with ethyl acetate to give a solid which was triturated with diethyl ether and filtered to give 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexanone, m.p. 252-254°C.

Example 176 and 177: cis-5-(4-phenoxyphenyl)-7-(4-morpholinocyclohex-l-yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine,-andfrans-5-(4-phenoxyphenyl)-7-(4-morpholinocyclohex-l-yl)-7H-pynolo[2,3-d] pyrimidin-4-ylamine
Sodium triacetoxyborohydride (42 mg) and glacial acetic acid (18 mg) were added to the product from the previous example (120 mg) and morpholine (31 mg) in 1,2 -dichloroethane. The mixture was stined at 40°C for 2 hours and then a further portion of morpholine (0.15 g) and sodium triacetoxyborohydride (0.21 g) were added. The mixture was stined at ambient temperature for 20 hours then quenched with saturated aqueous bicarbonate solution. The mixture was filtered through an EMPORE® cartridge and the filtrate was extracted with 3M hydrochloric acid. The acidic extracts were basified with 5M sodium hydroxide solution and extracted with dichloromethane to give a residue which was purified by chromatography on silica to give cis-5-(4-phenoxyphenyl)-7-(4-morpholinocyclohex-l-yl)-7H-pynolo[2,3- d]pyrimidin-4-ylamine, and trans-5-(4-phenoxyphenyl)-7-(4-morpholinocyclohex-l -yl)-7H-pynolo[2,3 -d] pyrimidin-4-ylamine.

Examples 178 and 179: cis-7-(4-N-ethoxycarbonyl)piperazin-l-ylcyclohexyl)-5-(4-phenoxy-phenyI)-7H-pynolo[2,3-d]pyrimidin-4-ylamine and trans-7-(4-N-ethoxycarbonyl)-piperazin-l-ylcyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
In a similar manner to the previous Example, 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexanone (0.4 g from 1.0 g of 40%) pure material) and 1-ethoxycarbonyl-piperidine (158 mg) were reacted together in the presence of sodium triacetoxyborohydride (296 mg) in
dichloromethane (15 ml) containing glacial acetic acid (60 mg) to give after workup and chromatography cis-7-(4-N-ethoxycarbonyl)piperazin-l-ylcyclohexyl)-5-(4-phenoxy-phenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine and trans-7-(4-N-ethoxycarbonyl)-piperazin-l-ylcyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidm-4-ylamine.

Example 180: 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]pyridine-3 -carbonitrile
5-(4-Phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine (906 mg) was reacted with 2-chloronicotinonitrile (510 mg) in the presence of sodium hydride (150 mg) in dimethylformamide (30 ml) at 100°C for 5 hours to give 2-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyriιmdin-7-yl]pyridine-3-carboι_itrile, m.p. 242-242.5°C, after workup.

Example 181: 7-[3-(aminomethyl)pyrid-2-yl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine dimaleate
The product from the previous example (468 mg), ethanol saturated with ammonia (200 ml) and Raney® nickel (2 ml) was shaken under hydrogen at a pressure of 26 bar at 80°C for 6 hours and then left standing at ambient temperature for 68 hours. The mixture was filtered and the residue was washed well with ethanol. The filtrate was evaporated under reduced pressure and the residue was taken up in ethyl acetate and filtered. Maleic acid (135 mg) dissolved in ethyl acetate (20 ml) was added in portions to the filtrate until no further precipitation occuned. The mixture was warmed and decanted from a small residual amount of gum. The gum was firrther heated with ethyl acetate and decanted. The combined ethyl acetate extracts were cooled and the solid which precipitated was collected by filtration to give 7-[3-(aminomethyl)pyrid-2-yl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine dimaleate, m.p. 131-134°C.

Example 182: 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-8-methyl-8-azabicyclo[3.2. l]octane
Sodium hydride (168 mg, of a 60% dispersion in mineral oil) was added to a mixture of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine (770 mg, in dimethylformamide (30 ml). 3-Mesyloxy-8-methyl-8-azabicyclo[3.2.1]octane

(900 mg, prepared as described in J.A.C.S. 1958, 80, 4679) in dimethylformamide (10 ml) was added under nitrogen with stirring. The mixture was warmed at 75°C for 5 hours (and left standing at ambient temperature for 7 days). The solvent was removed under reduced pressure. Water was added to the residue and the mixture was extracted with ethyl acetate to give a residue which was purified by flash column chromatography on silica using ethyl acetate/methanol (50:50) as the mobile phase to remove starting material and then a mixture of ethyl acetate/methanol triethylamine (5:5:1) as the mobile phase to elute the product. Appropriate fractions were combined and evaporated to give a solid which was triturated with ether and filtered to give 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-8-methyl-8-azabicyclo[3.2.1]octane, m.p. 238-250°C.

Examples 183 and 184: cis-7-(N-methylhomopiperazin-l-ylcyclohexyl)-5-(4-phenoxyphenyl)-7H-ρynolo[2,3-d]pιyrnidin-4-ylamine and trans 7-(N-methylhomo-piperazin-l-ylcyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]prymidin-4-ylamine In a similar manner to Examples 176 and 177, 4-[4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexanone (0.4 g from 1.0 g of a 40% pure material), N-methylhomopiperazine (114 mg), sodium
triacetoxyborohydride (296 mg), glacial acetic acid (60 mg) and 1,2-dichloroethane (15 ml) were reacted together. After filtration, the filtrate was evaporated and the residue was purified by chromatography on silica to give cis-7-(N- methylhomopiperazin- 1 -ylcyclohexyl)-5 -(4-phenoxyphenyl)-7H-pynolo [2,3 - d]prymidin-4-ylamine and trans 7-(N-methylhomo-piperazin-l-ylcyclohexyl)-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]prymidin-4-ylamine.

Examples 185 and 186: cis 7-(N-methylpiperazin-l-ylcyclohexyl)-5-(4- phenoxyphenyl)-7-pynolo[2,3-d]ρrymidin-4-ylamine and trans 7-(N- methylpiperazin-l-ylcyclohexyl)-5-(4-phenoxy-phenyl)-7-pynolo[2,3-d]prymidin-4- ylamine
In a similar manner to the previous Example, N-methylpiperazine (100 mg) was reacted with the same amounts of cyclohexanone derivative and other reagents to give cis 7-(N-methylpiperazin-l-ylcyclohexyl)-5-(4-phenoxyphenyl)-7-' pynolo[2,3-d]prymidin-4-ylamine and trans 7-(N-methylpiperazin-l-ylcyclohexyl)- 5-(4-phenoxy-phenyl)-7-pynolo[2,3-d]prymidin-4-ylamine.

Example 187: 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]- cyclopentan- 1 -one
A mixture of 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin- 7-yl]-cyclopentan-l-ol (100 mg), activated manganese dioxide (500 mg) and dichloromethane (100 ml) was stined at ambient temperature for 18 hours to give, after filfration, a solution of 3-[4-amino-5-(4-phenoxyphenyl)-7H-ρynolo[2,3- d]pyrimidin-7-yl]-cyclopentan-l-one in dichloromethane which was used in the next Example.

Example 188: cis-7-(3 -morpholinocyclopent- 1 -yl)-5 -(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine and trans-7-(3-morpholinocyclopent-l-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidin-4-ylamine
Morpholine (45 mg) was added to the solution obtained in the previous Example followed by sodium triacetoxyborohydride (151 mg) and glacial acetic acid (47 mg). The mixture was stined at ambient temperature under nitrogen for 18 hours during which time the dichloromethane evaporated. Tetrahydrofuran (100 ml) was added and the mixture was stined for a further 8 hours. The mixture was worked up to give cis-7-(3-morpholinocycloρent-l-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine and trans-7-(3-morpholinocyclopent-l-yl)-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]-pyrimidin-4-ylamine.

Example 189: 3-(4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)cyclopentyl N-(2-moφholinoethyl)-carbamate hydrochloride

a) To a solution of 3-(4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin- 7-yl)cyclopentanol (20 mg) in dichloromethane (1 ml) at 0°C was added N- methylmorpholine (7 ml) and the mixture stined for 20 minutes. The cooling bath was removed and 4-nitrophenylchlorofoιmate (12.5 mg) was added and the resulting mixture stined overnight at ambient temperature. The mixture was diluted with dichloromethane, washed with water, saturated aqueous sodium bicarbonate solution and brine. The organic solution was dried over magnesium sulphate and evaporated to give crude product.
b) The crude product from a) in dichloromethane (2 ml) was added to 2- morpholinoethylamine (0.2 ml) and the mixture stined overnight at ambient temperature. The mixture was diluted with ethyl acetate and washed with water and brine. The organics were dried, filtered and evaporated to give a crude product which was purified by preparative HPLC to give 3-(4-amino-5-(4- phenoxyphenyl)-7H- pynolo[2,3-d]pyrimidin-7-yl)cyclopentyl N-(2- morpholinoethyl)carbamate.

c) The product from b) was dissolved in ethyl acetate (2 ml) and hydrogen chloride gas was bubbled through the solution for 2 minutes. A precipitate formed and stirring was continued for a further 10 minutes. The solvent was evaporated and water added to dissolve the solid. Lyophilisation gave 3-(4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)cyclopentyl N-(2- morpholinoethyl)-carbamate hydrochloride as a solid.

Example 190: 3-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopenxyl 2-aminoacetate hydrochloride

a) 3-[4-Amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-7- yljcyclopentanol (50 mg, 0.129 mmol) and N-tert-butoxycarbonyl glycine (34 mg, 0.194 mmol) was mixed in N,N-dimethylformamide (1 ml). l-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (31 mg, 0.155 mmol) and 4-dimethylamino pyridine (16 mg, 0.129 mmol) was added. The resulting mixture was stined under nitrogen at ambient temperature for 24 hours. The reaction mixture was poured into ice water and extracted with ethyl acetate. The organic extracts were washed with brine, dried (MgSO4), filtered and
evaporated. The solid was purified by flash column chromatography on silica using ethyl acetate as the mobile phase to give 3-[4-amino-5-(4-phenoxyphenyl)- 7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl 2-[(tert- butoxycarbonyl)amino]acetate. The structure was confirmed by Η NMR.

b) 3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl 2-[(tert-butoxycarbonyl)amino]acetate (39 mg, 0.072 mmol) was disolved in ethyl ecetate (2.5 ml). Hydrogen chloride gas was passed through for 1 minute. The flask was capped and the solution stined for additional 30 minutes. Diethyl ether was added and precipitate formed. The solid was collected by filtration to give 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyιrimidin-7- yljcyclopentyl 2-aminoacetate hydrochloride. The structure was confirmed by :H NMR and LC/MS (M__T = 444).

Example 191 : 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cycloρentyl (2S)-2-amino-3-methylbutanoate hydrochloride

a) (2S)- 1 -[(tert-Butoxycarbonyl)amino]-2-methylbutanoic 2,5-dioxo-2,5-dihydro- lH-1-pynolecarboxylic anhydride ( 114 mg, 0.362 mmol) was added to a solution of 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7- yljcyclopentanol (66 mg, 0.171 mmol) in dichloromethane (1 ml). The resulting mixture was stined under nitrogen at ambient temperature for 24 hours. The reaction mixture was diluted with ethyl acetate and washed, dried (MgSO4), filtered and evaporated. The solid was purified by flash column chromatography on silica using ethyl acetate as the mobile phase to give 3-[4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl (2S)-2-[(tert- butoxycarbonyl)amino]-3-methylbutanoate. The structure was confirmed by lH

NMR and LC/MS (MH+ = 586).

b) 3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl (2S)-2-[(tert-butoxycarbonyl)amino]-3-methylbutanoate (35 mg, 0.060 mmol) was dissolved in ethyl acetate (2.5 ml). Hydrogen chloride gas was passed through for 5 minutes. The flask was capped and the solution stined for additional 30 minutes. Diethyl ether was added and precipitate formed. The solid was collected by filtration to give 3-[4-amino-5-(4-phenoxyphenyl)-7H- pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl (2S)-2-amino-3-methylbutanoate hydrochloride. The structure was confirmed by Η NMR and LC/MS (MET =

486).

Example 192: 3-(4-anιino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)cyclopentyl N-(2-moφholinoethyl)carbamate hydrochloride

a) N-Methylmoφholine (0.007 ml, 0.062 mmol) was added dropwise to solution of 4-nitrophenyl chloroformate (12.5 mg, 0.062 mmol) in dichloromethane (1 ml) with stirring under nitrogen at 0°C. After 20 minutes, the ice- water bath was removed and the mixture was allowed to warm up to ambient temperature. 3-[4- amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentanol (20 mg, 0.052 mmol) was added to the mixture and the resulting solution was stined for 24 hours. The reaction mixture was diluted with dichloromethane and washed with water, saturated sodium bicarbonate, and brine. The organic layer was dried (MgSO4), filtered and evaporated to give 3-[4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl (4-nitrophenyl) carbonate. The structure was confirmed by *H NMR.

b) 3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopenxyl (4-nitrophenyl) carbonate (0.052 mmol) in dichloromethane (1 ml) was added to 2-moφholinoethylamine (0.2 ml). The resulting mixture was stined under nitrogen at ambient temperature for 24 hours. The reaction mixture was diluted with ethyl acetate and washed, dried (MgSO4), filtered and evaporated. The solid was purified by preparative HPLC to give 3-[4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl N-(2- moφholinoethyl)carbamate. The structure was confirmed by ]H NMR and LC/MS (MET = 543).

c) 3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl N-(2-moφholinoethyl)carbamate (10 mg, 0.018 mmol) was dissolved in ethyl acetate (2.5 ml). Hydrogen chloride gas was passed through for 2 minutes, and a precipitate formed. The flask was capped and the solution stined for additional 10 minutes. The solid was collected by filfration to give 3-(4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)cyclopentyl N-(2- moφholinoethyl)carbamate hydrochloride. The structure was confirmed by !H NMR and LC/MS (MET = 543).

Preparation of Starting Materials

a) Tert-butylamine (15 ml) was added with stirring to a solution of 2-bromo-4'- phenoxyacetophenone (12.7 g, prepared by bromination of 4'- phenoxyacetophenone according to Tetrahedron Letters, 1993, 34. 3177) in propan-2-ol and the mixture heated at 80°C for 3 hours. The mixture was cooled to 0°C and concentrated hydrochloric acid (10 ml) added. The suspension was stined at ambient temperature for 18 hours and the solid collected by filtration to give 4'-phenoxy-2-(tert-butylamino)acetophenone hydrochloride (3.75 g), m.p. 210-212°C.

b) (1) 4'-Phenoxy-2-(tert-butylamino)acetophenone hydrochloride (3.75 g) was added in one portion to sodium ethoxide (prepared by dissolving sodium
(93 mg) in ethanol (50 ml)) and the mixture was stined at 40°C for 30
minutes under nitrogen.

(2) In a separate flask sodium (331 mg) was dissolved in ethanol (50 ml) and malononixrile (858 mg) was added. The solution was stined at ambient temperature for 5 minutes and then to this solution was added the solution of 4'-phenoxy-2-(tert-butylamino)acetophenone obtained in part (1) in one portion excluding the precipitated sodium chloride. The resultant mixture was heated at 50°C for 3 hours and then at 80°C for 2 hours. The solvent was removed under reduced pressure and the resultant oil was partitioned
between water and ethyl acetate. The organic phase was separated, dried and evaporated to give a black solid. This solid was dissolved in hot ethanol and triturated with water, filtered and dried to give 2-amino-3-cyano-4-(4- phenoxyphenyl)- 1 -(tert-butyl)pynole.

c) A mixture of 2-amino-3-cyano-4-(4-phenoxyphenyl)-l-(tert-butyl)pynole (1.9 g), formamide (30 ml) and 4-dimethylaminopyridine (10 mg) was heated at 180°C for 6 hours. The mixture was cooled to ambient temperature and water was added to precipitate a dark solid. The solid was collected by filtration, washed with water, then boiled up in ethanol and the insoluble material collected by hot filtration and dried. The solid was purified by preparative HPLC on a silica column using dichloromethane/propan-2-ol/ ethanol, 98:1:1 as the mobile phase to give 7-tert-butyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4- ylaιnine (4-amino-5-(4-phenoxyphenyl)-7-(tert-butyl)pynolo[2,3-d]pyrimidine), m.p. 157-158°C. IH NMR (d6 DMSO) δ 8.15 (lH,s), 7.50-7.35 (4H,m), 7.30 (lH,s), 7.15 (lH,t), 7.10 (4H,m), 6.05 (2H,brs), 1.75 (9H,s).

d) A mixture of 4-amino-5-(4-phenoxyphenyl)-7-(tert-butyl)ρynolo[2,3-d]- pyrimidine (5.8 g), glacial acetic acid (55 ml) and hydrobromic acid (55 ml of a 48%) solution) was boiled under reflux for 18 hours under nitrogen. The mixture was allowed to cool and a solid was collected by filtration. This solid was washed with methanol and then with ether to give 4-amino-5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]-pyrimidine hydrobromide, m.p. 288-292°C.

The hydrobromide salt was converted into the free base by warming with dilute sodium hydroxide solution (100 ml of 5% w/v solution) and ethanol (60 ml) with stirring and removing the ethanol by distillation. The mixture was cooled and the solid was collected by filtration and washed well with water to give 5-(4- phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine.

e) A mixture of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyι__midin-4-ylamine (600 mg) and tetrakis(triphenylphosphine) palladium (40 ml) and dry dimethyl sulphoxide (30 ml) was stined under nitrogen in an ice/water bath and then a solution of cyclopentadiene monoepoxide (200 mg) in tetrahydrofuran (10 ml) was added via syringe under nitrogen at 0°C. The mixture was stined at ambient temperature (with exclusion of light) for 66 hours and then the tetrahydrofuran was removed under reduced pressure and water was added to the residue. The mixture was allowed to stand for 18 hours and then extracted with ethyl acetate to give a residue which was purified by flash column chromatography on silica using ethyl acetate/industrial methylated spirit (9:1) as the mobile phase to give 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-cyclopent-2- enol, as an oil. The structure was confirmed by iHnrnr and mass spectra.

f) 4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopent-2- enol (110 mg) was hydrogenated in ethanol (20 ml) with gaseous hydrogen at atmospheric pressure using 10% palladium on charcoal (50 mg) as the catalyst. The catalyst was removed by filtration and the filtrate was evaporated to give 3- [4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentanol, as an oil. The structure was confirmed by IH nmr and mass spectra.

Example 193: Cis-5-(4-phenoxyphenyl)-7-(4-pynolidinocyclohex- 1 -yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
Trans-5-(4-phenoxyphenyl)-7-(4-pynolidinocyclohex-l-yl)-7H-pynolo[2,3-d]pyrimidm-4-ylamine
To a stined suspension of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidinyl-7-yl]cyclohexanone (2.34 g, 5.9 mmol) in 1,2 dichloroethane (250 mL) was added, under an atmosphere of nitrogen, pyπolidine (1.25 g, 17.6 mmol) and glacial acetic acid ( 1.00 mL, 17.6 mmol), and the resultant mixture stined at room temperature for 30 minutes. Sodium triacetoxyborohydride (1.87 g, 8.8 mmol) was added in one portion, and the resultant mixture stined for 70 hours. The mixture was extracted with 2M aqueous hydrochloric acid (2 x 200 mL). The combined extracts were washed with dichloromethane (300 mL), made basic with 12.5M aqueous sodium hydroxide solution, and extracted with dichloromethane (3 x 200 mL). The combined extracts were dried over sodium sulphate, and purified by chromatography with a Biotage 40S column using ethyl acetate / triemylamine (95:5) and ethyl acetate / triethylamine / methanol (85:10:5) as a mobile phase to yield Cis-5-(4-phenoxyphenyl)-7-(4-pynolidinocyclohex-l-yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine as an off-white solid (0.65 g, 1.4 mmol), melting point 101 — 104 deg.C, LC/MS Hypersil BDS cl8 (100 x 2.1 mm) 0.1M
ammoniumacetate/acetonitrile, 10-100% acetonitrile in 8 min.): MH+ 454 tr= 3.56 minutes and Trans-5-(4-phenoxypheny l)-7-(4-pynolidinocyclohex- 1 -yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine as an off-white solid (0.93 g, 2.1 mmol), melting point 183 - 185 deg.C, LC/MS (Hypersil BDS cl8 (100 x 2.1 mm) 0.1M
ammoniumacetate/acetonitrile, 10-100%) acetonitrile in 8 min.): MH+ 454 , t,. = 3.68 minutes

Example 194: Cis-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohex-l-yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine hydrochloride
Trans-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohex-l-yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
To a stined suspension of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin-7-yl]cyclohexanone (2.34 g, 5.9 mmol) in 1,2 dichloroethane (250 mL) was added, under an atmosphere of nitrogen, piperidine (1.50 g, 17.6 mmol) and glacial acetic acid ( 1.00 mL, 17.6 mmol), and the resultant mixture stined at room temperature for 30 minutes. Sodium triacetoxyborohydride (1.87 g, 8.8 mmol) was added in one portion, and the resultant mixture stined for 70 hours. The mixture was extracted with 2M aqueous hydrochloric acid (2 x 200 mL). The combined extracts were washed with dichloromethane (300 mL), made basic with 12.5M aqueous sodium hydroxide solution, and extracted with dichloromethane (3 x 200 mL). The combined extracts were dried over sodium sulphate, and purified by chromatography with a Biotage 40S column using ethyl acetate / triethylamine (95:5) as a mobile phase to yield Cis-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohex-l-yl)-7H-pynolo[2,3-d]pyri_nidin-4-ylamine (0.23 g) as a clear oil., LC/MS :ypersil BDS cl8 (100 x 2.1 mm) 0.1M ammoniumacetate/acetonitrile, 10-100%) acetonitrile in 8 min.) MH+ 468 1_ = 3.67 minutes and Trans-5-(4-ρhenoxyphenyl)-7-(4- piperidmocyclohex-l-yl)-7H-pynolo[2,3-d]pyriιrndin-4-ylamine as an off-white solid (193 mg, 0.4 mmol), melting point 192 - 195 deg.C, LC/MS: Hypersil BDS cl8 (100 x 2.1 mm) 0.1M ammoniumacetate/acetonitrile, 10-100%) acetonitrile in 8 min.)MH+ 468 t_= 3.71 minutes

Example 195 : Cis-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohex- 1 -yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine was dissolved in ethyl acetate (50 mL), diluted with diethyl ether (50 mL) and treated with a IM solution of hydrogen chloride in diethyl ether until no further precipitation occuπed. The resultant solid was collected and re-crystallised from absolute ethanol to give Cis-5-(4-ρhenoxyphenyl)-7-(4-piperidinocyclohex-l-yl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine hydrochloride as a colourless solid (75 mg, 0.2 mmol) melting point 185 - 189 deg.C.

Example 196: Trans-7-(4-dimethylaminocyclohexyl)-5-(4-ρhenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-4-ylamine
Cis-7-(4-dimethylaminocyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
To a stined solution of 4-[4-amino-5-(4-phenoxyphenyl)-7H-ρynolo[2,3-d]pyrimin-7-yl]cyclohexanone (3.24 g, 8.1 mmol) in dichloromethane (1000 mL) was added, under an atmosphere of nitrogen, N-methylpiperazine (1.20 g, 12.0 mmol) and glacial acetic acid ( 0.69 mL, 12.0 mmol), and the resultant solution stined at room temperature for 10 minutes. Sodium triacetoxyborohydride (1.70 g, 8.0 mmol) was added in one portion, and the resultant solution stined for 6 hours. The additions were repeated on the same scale and the resultant solution stined for 70 hours. The solution was extracted with 2M aqueous hydrochloric acid (2 x 300 mL). The combined extracts were washed with dichloromethane (300 mL), made basic with .880 aqueous ammonia solution, and extracted with ethyl acetate (3 x 250 mL). The combined extracts were washed with saturated aqueous sodium chloride solution, dried over sodium sulphate, and purified by chromatography with a Biotage 40M column using ethyl acetate / methanol / triethylamine (8 : 1 : 1) as a mobile phase to yield Cis-7-(4-dimethylaminocyclohexyl)-5-(4-phenoxyphenyl)- 7H-pynolo[2,3-d]pyrimidin-4-ylamine as an off-white solid (220 mg, 0.5 mmol.) melting point 180 - 182 deg.C, LC/MS: Hypersil BDS cl8 (100 x 2.1 mm) 0.1M ammoniumacetate/acetomtrile, 10-100% acetonitrile in 8 min^MEr1" 428 t-= 3.43 minutes
The column was flushed with ethyl acetate / methanol / triethylamine (4:1:1,

500 mL), and the solvent removed under reduced pressure. The residue was dissolved in dichloromethane (200 mL)and purified by chromatography with a Biotage 40M column using dichloromethane/methanol (9:1 to 7:3) to yield Trans-7-(4-dimethylammo-cyclohexyl)-5-(4 henoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine as an off-white solid (320 mg, 0.75 mmol) melting point 207.5 - 210 deg. C, LC/MS: Hypersil BDS cl8 (100 x 2.1 mm) 0.1M ammoniumacetate/acetonitrile, 10-100% acetonitrile in 8 min.)MH+428 t_ = 3.48 minutes
R - (+) - 4 -[4-amino-5-(4-phenoxyphenyl)-7- (3-tefrahydrofuryl)-7H-pyπolo[2,3-d]pyrimidine.

Example 197: 4 -{(S) - tetrahydrofuran-3-yl}toluenesulphonate
To a solution of (S)-3-hydroxytetrahydrofuran (2.0 g, 23 mmol) in pyridine (40 ml) at 0°C was added tosylchloride portionwise (4.8 g, 25 mmol). The solution was stined at 0°C for 1 hr and then at room temperature overnight. The pyridine was evaporated in vacuo and the residue was partioned between EtOAc and saturated aquoeus citric acid (200 ml each). The aqueous layer was extracted with EtOAc (2 x 200 ml) and the combined organics were dried (sodium sulphate), filtered and evaporated to leave an oil (4.5 g, 85%). 'H NMR (CDC13, 250 MHz): 7.78 (2H, d), 7.35 (2H, d), 5.12 (IH, m), 3.76-3.93 (4H, m), 2.45 (3H, s), 2.01-2.20 (2H, m).
To a stined suspension of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine (4.83 g, 16 mmol) in N,N-dimethylformamide (80 mL), under an atmosphere of nitrogen, was added 60% sodium hydride in mineral oil (0.75 g, 19 mmol), and the mixture stined at room temperature for 30 minutes. The resultant dark solution was treated with a solution of 4 -{(S) - tetrahydrofuran-3-yl}toluenesulphonate (4.20 g, 18 mmol) in N,N-dimethylformamide (20 mL) in 2 mL aliquots. The resultant solution was stined at room temperature for 30 minutes, then at 95 deg.C. for 18 hours. The solution was allowed to cool to ambient temperature, then poured onto ice/water (200 mL). The aqueous was extracted with ethyl acetate (3 x 200 mL). The combined organic extracts were washed with water (4 x 150 mL), dried over sodium sulphate, and the solvent was removed under reduced pressure. The residue was warmed with dichloromethane (1000 mL) until a solution was obtained, cooled to ambient temperature, and purified by
chromatography with a Biotage 40M column using ethyl acetate/ triethylamine (95:5), then ethyl acetate / Iriethylamine / methanol (90:5:5) as a mobile phase, to yield R - (+) - 4 -[4-amino-5-(4-phenoxyphenyl)-7- (3-tetrahydrofuryl)-7H-pyπolo[2,3~d]pyrimidine as an off-white solid (4.35 g, 12 mmol) melting point 165-166 deg. C, LC/MS : Hypersil BDS cl8 (100 x 2.1 mm) 0.1M
ammom'umacetate/acetonitrile, 10-100% acetonitrile in 8 min.) MH+ 373 tr = 4.44 minutes. []D + 20.5 + 0.6 (dichloromethane, 22.6 deg.C.)

Example 198: 5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-4-ylamine
N-tert-butoxycarbonylpiperidinol
To a solution of N-tert-butoxycarbonylpiperidone (10.0 g, 50 mmol) in MeOH (100 ml) at 0°C was added sodium borohydride (1.9 g, 50 mmol) portionwise. Stir at 0°C for 1 hr and then at room temperature for 20 hr. Quench with 2N NaOH (20 ml), evaporate solvent and partition residue between ethylacetate and water (100 ml each). Extract the aqueous layer with ethylacetate (3 x 100ml) and wash the combined organic layers with brine and water (1 x 100 ml each). Dry (Na^O , filter and concentrate to leave N-tert-butoxycarbonylpiperidinol as a colourless oil (10.5 g, 100%). R_ in 20% EtOAc / hexane = 0.05 ( MnO4 dip). IR (thin film) : 3428, 2939, 1693 cm"1

Example 199: tert-butyl 4-[(4-methylphenyl)sulfonyl]oxy-l-piperidinecarboxylate To a solution of N-tert-butoxycarbonylpiperidinol (10.5 g, 0.052 mol) in pyridine (150 ml) at 0°C under nifrogen was added tosylchloride (9.94 g, 0.052 mol) portionwise. Stir at 0°C for 2 hr. Warm to room temperature and stir at room temperature overnight. Evaporate the solvent and partition between citric acid solution (IM, 100 ml) and ethylacetate (200 ml). Extract acidic layer with ethylacetate (1 x 100 ml) and wash combined organics with citric acid solution (IM, 2 x 100 ml), brine (100 ml) and water (100 ml). Dry (N&fiO , filter and evaporate to leave an oil which was purifed by flash column chromatography using 10% EtOAc / cyclohexane then 15% EtOAc / cyclohexane to give in F 30-68 tert-butyl 4-[(4-methylphenyl)sulfonyl]oxy-l-piperidinecarboxylate as a white solid (11.0 g, 60%) Rf in 20% EtOAc / cyclohexane = 0.17 Η NMR (CDC13, 250 MHz) : δ 7.79 (2H, d ), 7.34 (2H, d), 4.67 (IH, m), 3.58 (2H, m), 3.27 (2H, m), 2.45 (3H, s), 1.59 -1.83 (4H, m), 1.43 (9H, s)

Example 200: tert-butyl 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]- 1 -piperidinecarboxylate
To a solution of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine

(2.0 g, 6.6 mmol) in dry DMF (100 ml) under nitrogen at OoC was added NaH (0.264 g, 60%) dispersion, 6.6 mmol) and the reaction mixture warmed to room temperature and stined for lhr. Tert-butyl 4-[(4-methylphenyl)sulfonyl]oxy-l-piperidinecarboxylate (2.34 g, 6.6 mmol) was added and the resulting solution heated at 95°C for 72 hr. The reaction was quenched by careful addition of water (150 ml). Extract with EtOAc (3 x 100 ml) and wash with water (4 x 100 ml) and brine (2 x 100 ml). The organic solution was dried (Na^O , filtered and evaporated to leave a solid which was adsorbed onto silica and purified by flash silica gel column chromatography using EtOAc then 5% MeOH/EtOAc as eluent to give in F 13-22 tert-butyl4-[4-amino-5-(4-phenoxyphenyl)-7H-ρynolo[2,3-d]ρyrimidin-7-yl]-1 -piperidinecarboxylate (1.0 g, 31%) as a white solid, m.pt. 168.5-l69.5oC. Rfin 10% EtOAc MeOH = 0.4 . Η NMR (d6 DMSO, 250 MHz) : δ 8.14 (IH, s), 7.38-7.49 (5H, m), 7.07-7.23 (5H, m), 6.14 (2H, bs), 4.76 (IH, m), 4.11 (2H, m), 2.93 (2H, m), 1.92-2.02 (4H, m), 1.43 (9H, s). Mass spec. C28H31O3N5 (485.2430).IR (KBr disc) : 3059, 1695, 1588, 1235 cm'1 Example 201 : 5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-4- ylamine
To a solution of tert-butyl 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3- d]ρyrimidin-7-yl]-l -piperidinecarboxylate (0.69 g, 1.4 mmol) in dry CH2C12 (25 ml) at 0°C was added TFA (5 ml). The solution was stined at room temperature for 20 hr and the solvent evaporated. NaOH solution (5N, 10 ml) was added and the resulting slurry was extracted with EtOAc (3 x 50 ml). Wash with brine (1 x 50 ml). Dry, filter and concentrate to leave a solid which was triturated with diethylether and filtered to leave 5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pyπolo[2,3- d]pyrimidin-4-ylamine (433258) as a white solid (500 mg, 91%). M.pt 209-211°C. Rfin 1:1 EtOAc : MeOH = 0.1. . Η NMR (d6 DMSO, 250 MHz) 8.13 (IH, s), 7.36- 7.48 (4H, m), 7.29 (IH, s), 7.04-7.16 (5H, m), 5.80 (2H, bs), 4.64 (IH, m), 3.10 (2H, m), 2.80 (IH, bs), 2.67 (2H, m), 1.94 (4H, m). Mass spec. C23H23ON5 (385.1902). IR (KBr disc) : 3278, 1620, 1585, 1490, 1245 cm"1

" Example 202: 5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-4- ylamine dihydrochloride
To 5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-4- ylamine (433258) (200 mg) in EtOAc/MeOH (15 ml, 1:1) was added ether.HCl solution (1.0 M, 3 ml). The resulting white precipitate was filtered under a stream of nitrogen and dried in vacuo for 6 hr to leave 5-(4-phenoxyphenyl)-7-(4-piperidyl)- 7H-pynolo[2,3-d]pyrimidin-4-ylamine dihydrochloride (1.4 hydrate) as a white solid (120 mg), m.pt. 304°C (dec). Η NMR (D2O, 250 MHz) 8.48(1H, s), 7-69 (IH, s), 7.50-7.58 (4H, m), 7.18-7.34 (5H, m), 5.16 (IH, m), 3.81 (2H, d), 3.46 (2H, m),

2.49 (4H, m). ). IR (KBr disc) : 3937, 1657. 1231 cm"1 Example 203: tert-butyl 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyιimidin-7-yl]-l-pynolidinecarboxylate
N-tert-butoxycarbonylpynolidin-3-ol
To a solution of pynolidin-3-ol (10.0 g, 0.11 mol) in dichloromethane (200 mL) was added triethylamine (22.2 g, 30.5 ml, 0.22 mol) followed by di-tert-butyldicarbonate (28.8 g, 0.13 mol) at 0°C. Warm to room temperature and stir at room temperature overnight. Quench with saturated aqueous citric acid (150 ml) amd wash the organic layer with water, brine and water again (1 x 100 ml each). The organic layer was dried (sodium sulphate), filtered and evaporated to leave N-tert-butoxycarbonylpynolidin-3-ol (20.0g, 93% crude) as a golden oil.

Example 204: tert-butyl 3-[(4-methylphenyl)sulfonyl]oxy-l-pynolidinecarboxylate To a solution of N-tert-butoxycarbonylpynolidin-3-ol (19.8 g, 0.106 mol) in pyridine (200 ml) at 0°C under nitrogen was added tosyl chloride (22.3 g, 0.117 mol) portionwise. Stir at 0°C for 2 hr, warm to room temperature and stir at room temperature overnight. The pyridine was evaporated in vacuo and the residue was partioned between EtOAc and saturated aquoeus citric acid (200 ml each). The aqueous layer was extracted with EtOAc (2 x 200 ml) and the combined organics were dried (sodium sulphate), filtered and evaporated to leave an oil which was purified by flash silica gel column chromatography using 10%> EtOAc/cyclohexane as eluent to give in F40-85 an oil. The oil was dissolved in a small volume of cyclohexane/ diethylether (5:1, 50 ml), cooled and scratched with a spatula to induce crystallisation. The resulting solid was filtered to give tert-butyl 3-[(4-methylphenyl)sulfonyl]oxy-l-pynolidinecarboxylate (10.5 g, 29%) as a white solid. Rf in EtOAc/cyclohexane = 0.13. Η NMR (CDC13, 250 MHz): 7.79 (2H, d), 7.35 (2H, d), 5.04 (IH, ), 3.43 (4H, m), 2.46 (3H, s), 2.03-2.20 (2H, bm), 1.43 (9H, s)

To a solution of 4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrrmidine (2.0 g, 6.6 mmol) in dry DMF (120 ml) under nitrogen at 0°C was added NaH (0.264 g, 60%) dispeersion, 6.6 mmol) and then reaction mixture warmed to room temperature and stined for lhr. tert-butyl 3-[(4-methylphenyl)sulfonyl]oxy-l-pynolidinecarboxylate (2.25 g, 6.6 mmol) was added portionwise and the mixture heated at 95°C for 72 hr. Quench with water and extract with EtOAc (4 x 100 ml). Wash the combined organic solutions with water (4 x 100 ml) and brine (2 x 100 ml). The organics were dried (sodium sulphate), filtered and evaporated to leave a solid which was dissolved in EtOAc/MeOH and adsorbed onto silica. Purification using flash silica gel column chromatography with 5% MeOH/ EtOAc as eluent gave in F17-25 tert-butyl 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l-pynolidinecarboxylate (1.0 g, 32%>) as a white solid m.pt. 168-170°C. 1^ in 9:1 EtOAc: MeOH = 0.46. ΗNMR (d6 DMSO, 250 MHz): 8.17 (IH, s), 7.38 -7.50 (5H, m), 6.19 (2H, bs), 5.31 (IH, m), 3.77 (IH, m), 3.42-3.60 (3H, m), 2.38 (2H, m), 1.40 (9H, s). Mass spec. 471.2250 (C27H29O3N5) IR (KBr disc): 3130, 1683, 1585, 1404, 1245 cm'1

Example 205: 5-(4-phenoxyphenyl)-7-(3-pynolidinyl) -7H-pynolo[2,3-d]pyrimidin-4-ylamine
To a solution of tert-butyl 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l-pynolidinecarboxylate (0.8 g, 1.7 mmol) in dichloromethane (25 ml) at 0°C was added trifluoroacetic acid (5 ml). The reaction mixture was warmed to room temperature and stined at room temperature for 20 hr. The solvent was evaporated and dilute NaOH added (5N, 10 ml). The resulting residue solution was extracted with EtOAc (3 x 50 ml) and the combined organics were washed with brine (1 x 75 ml). The organic solution was dried (sodium sulphate), filtered and evaporated in vacuo to leave 5-(4-phenoxyphenyl)-7-(3-pynolidinyl) -7H-pynolo[2,3-d]pyrimidin-4-ylamine as a white solid (0.5 g, 79%.) m.pt. 182-184°C. Rf in 1:1 EtOAc : MeOH = 0.15. Η NMR (d6 DMSO, 250 MHz): 8.14 (IH, s), 7.37-7.50 (5H, m), 7.05 - 7.18 (5H, m), 6.14 (2H, bs), 5.23 (IH, m), 3.09 -3.27 (2H, m), 2.83-2.98 (2H, m), 2.19-2.33 (IH, m), 1.88-2.01 (IH, m). Mass spec. 371.1758 (C22H21ON5). IR (KBr disc): 3106, 1585, 1489, 1232 cm'1 Example 206: 5-(4-phenoxyphenyl)-7-(3-pynolidinyl) -7H-pynolo[2,3-d]pyrimidin-4-ylamine dihydrochloride
To a solution of 5-(4-phenoxyphenyl)-7-(3-pynolidinyl) -7H-pynolo[2,3-d]ρyrimidin-4-ylamine (200 mg) in EtOAc/MeOH (2:1, 20 ml) was added ether.HCl (1.0 M, 3 ml) and the resulting precipitate was filtered under nitrogen to give 5-(4-phenoxyphenyl)-7-(3-pynolidinyl) -7H-pynolo[2,3-d]pyrimidin-4-ylamine dihydrochloride (0.4 hydrate) as a white solid (190 mg) m.pt. 298°C (dec). IR (KBr disc): 2909, 1658, 1249 cm'1

Example 207: 7-perhydro-l-pynolizinyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3,d] pyrimidin-4-amine dihydrochloride salt

a) perhydro-1-pynolizinol

Prepared as described by Schnekenburger J, Briet E, Arch. Pharm. (Wienheim) 310, 152-160 (1977).

b) perhydro-1-pynohzinyl methanesulfonate

A mixture of perhydro-1-pynolizinol (0.5 g, 3.94 mmol) and triethylamine (0.60 g, 5.91 mmol) in dichloromethane (10 ml) was stined at 0°C under an atmosphere of nitrogen. Methanesulfonyl chloride (0.68 g, 5.91 mmol) was added, then the mixture was allowed to warm to ambient temperature and stined for 8 hours. Saturated aqueous ammonium chloride (10 ml), dichloromethane (25 ml) and saturated aqueous sodium bicarboante (10 ml) were added. The organic layer was dried over magnesium sulfate filtered and the filtrate evaporated under reduced pressure to give a residue. Purification ofthe material by flash chromatography on silica gel using heptane/ethyl acetate (1 :3) as an eluent yielded perhydro-1-pynolizinyl
methanesulfonate (0.54 g): Η NMR (DMSO-d6, 400MHz) δ 4.96 (m, IH), 3.61 (m, IH), 2.9-3.3 (m, 6H), 2.35 (m, IH), 1.55-2.25 (m, 6H).

c) 7-perhyo o-l-pynolizinyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3,d]pyrimid_n-4-amine dihydrochloride salt
A mixture of 5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrhnidin-4-amine (0.49g, 1.62 mmol) and 60% sodium hydride in oil (100 mg, 2.43 mmol) in DMF was stined at ambient temperature for 15 minutes under an atmosphere of nitrogen . The mixture was heated at 100°C for 18 hours then cooled to ambient temperature. Additional 60%) sodium hydride in oil (100 mg, 2.43 mmol) was added and heating continued for another 2 hours. The mixture was cooled to ambient temperature and the solvents removed under reduced pressure. The residue was partitioned between water (10 ml) and dichloromethane (30 ml). The organic layer was dried over magnesium sulfate, filtered and the solvent was removed from the filtrate under reduced pressure. The resulting residue was purified by preparative C-18 RP HPLC to give 150 mg of white solid which was dissolved in ethyl acetate (10 ml) and treated with 1 N hydrogen chloride in diethyl ether to give 7-perhydro-l-pynolizinyl-5-(4-phenoxyphenyl)-7H-pynolo[2,3,d] pyrimidin-4-amine
dihydrochloride salt as a white solid: Η NMR (DMSO-d6, 400MHz) δ 8.52 (s, IH), 7.95 (s, IH), 7.02-7.58 (m, IH), 5.38 (m, 1H0, 4.40 (m, IH), 1.9-3.9 (m, 10H); (Hypersil HS C18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t=7.62 min; MS: MH+ 412.

Example 208 : 7-(2-methylperhydrocyclopenta[c]pynol-5-yl)-5-(4-phenoxyphenyl)-7H-ρyπolo[2,3-d]pyrimidin-4-amine dihydrochloride salt

a) 2-methylperhydrocyclopenta[c]pynol-5-ol
Prepared as described by Bohme H, Setiz G, Arch. Pharm. (Wienheim) 301,.341 (1968).

b) 4-chloro-5-iodo-7-(2-methylperhydrocyclopenta[c]pynol-5-yl)-7H-pynolo[2,3- d]pyrimidine

A mixture of 4-chloro-5-iodo-7H-pynolo[2,3-d]pyrimidine (0.38 g, 1.36 mmol), 2-methylperhydrocyclopenta[c]pynol-5-ol (0.23 g, 1.63 mmol) and
triphenylphosphine (0.71 g, 2.72 mmol) in tetrahydrofuran (20 mL) was treated with diethylazodicarboxylate (0.474 g, 2.72 mmol) and stined for 2 hours at ambient temperature. The solvent was removed under reduced pressure and the residue was partitioned between dichloromethane (30 ml) and water (10 ml). The organic layer was washed with saturated aqueous sodium chloride (10 ml) then dried over magnesium sulfate then filtered and the filtrate evaporated under reduced pressure to give a residue. The residue was purified by flash chromatography on silica using dichloromethane/ methanol (8:2) as mobile phase to yield 4-chloro-5-iodo-7-(2-methylperhydrocyclopenta[c]pynol-5-yl)-7H-ρynolo[2,3-d]pyrimidine (0.25 g): lH NMR (DMSO-d6, 400MHz) δ 8.62 (s, IH), 7.44 (s, IH), 7.26 (s, 2H), 5.36 (m, IH), 2.88 (m, 2H), 2.68 (m, 2H), 2.43 (m, 2H), 2.36 (s, 3H), 2.06-2.02 (m, 4H); TLC (dichloromethane/methanol 8:2) Rf = 0.29; RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) tr=6.50 min; MS: MH+ 403.

c) 7-(2-methylperhydrocyclopenta[c]pynol-5-yl)-5-(4-phenoxyphenyl)-7H- pynolo[2,3-d]pyrimidin-4-amine dihydrochloride salt
A mixture of 4-chloro-5-iodo-7-(2-methylperhydrocyclopenta[c]pynol-5-yl)-7H-pynolo[2,3-d]pyrimidine (0.25 g, 0.622 mmol), 4-phenoxyphenyl boronic acid (0.16 g, 0.746 mmol), tetrakis(triphenylphosphine)palladium (0.043 g, 0.037 mmol) and sodium carbonate (0.172 g, 1.62 mmol) was heated in a mixture of ethylene glycol dimethyl ether (8 mL) and water (4 mL) at 90° C for 18 hours under an atmosphere of nitrogen. The mixture was allowed to cool to ambient temperature and solvents were removed under reduced pressure. The residue was partitioned between water (10 mL) and dichloromethane (30 ml) The layers were separated and the organic solution was dried over magnesium sulfate, filtered and the filtrate concentrated to a residue under reduced pressure (0.354 g). The material was dissolved in 1,4-dioxane (10 ml) and concentrated (28 >) ammonium hydroxide (10 ml). The mixture was heated in a sealed tube at 120°C for 20 hours then cooled to ambient temperature.

The solvents were evaporated under reduced pressure then purified by flash column chromatography on silica using dichloromethane/methanol 7:3) as an eluent to give 7-(2-methylperhydrocyclopenta [c]pynol-5-yl)-5-(4-phenoxyphenyl)-7H-ρyπolo[2,3-d]pyrimidin-4-amine (0.05 g): Η NMR (DMSO-d6, 400MHz) shows two sets of peaks due to the cis and trans isomers ofthe desired compound δ 10.6-10.8 (bs, IH), 8.49 (s, IH), 6.99-7.98 (m, 11H), 5.39 and 5.48 (m, IH), 2-3.8 (m, 10H); PH 454098: RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml min ) ^=7.53 min; MS: Mrf" 426. The dihydrochloride salt of 7-(2-methylperhydrocyclopenta[c]pyπol-5-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine was prepared by dissolving the free base in 10 ml 1 N hydrochloric acid and lyophilizing.

Example 209: Cis and trans-7-[4-(N-tert-butoxycarbonyl-lS, 4S-2,5-diaza[2.2.1] heptanyl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine
A suspension of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l-cyclohexanone (0.67 g, 1.68 mmol) in dichloroethane (40 ml) was treated with tert-Butyl (IS, 4S)-(-) 2,5-diazabicyclo[2.2.1]heptane-2-carboxylate (1.0 g, 5.04 mmol) and glacial acetic acid (0.30 g, 5.04 mmol) at room temperature for 1 h. Subsequently, Na(OAc)3BH (0.46 g, 2.17 mmol) was added and stined for 8 days at 80°C. To the cooled reaction solution, a solution of NaHCO3 (0.377 g, 10.08 mmol) in water (15 ml) was added and stined for 15 min. The layers were separated and the organic layer was washed with water and brine ( 3 x 100 ml each ). The aqueous layer was extracted with CH2C12, the organic layers combined , dried (MgSO4), filtered and concenfrated. The solid was purified by flash silica gel column chromatography , (2 L, 6% MeOH in CH2C12, then 2 L 10% MeOH / 5% NH4OH in CH2C12 ) to give :

Example 210: Cis-7-[4-(N-tert-butoxycarbonyl-lS, 4S-2,5-diaza[2.2.1]heptanyl) cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyιimidin-4-amine (605 mg, lH NMR (d6 DMSO, 400 MHz) : δ 8.13 (IH, s), 7.39-7.49 (4H, m), 7.32 (IH, m), 7.07-7.17 (5H,m), 6.09 (2H, bs), 4.63 (IH, m), 4.15 (IH, m), 3.30-3.70 (2H, ), 3.03-3.08 (2H, m), 2.80-2.90 (IH, m), 2.70-2.75 (IH, m), 2.29-2.35, (IH, m), 2.09-2.21 (IH, m), 1.81-1.93 (4H, m), 1.60-1.80 (4H, m), 1.39 (9H, m).
HPLC/MS: Perkin Elmer Pecosphere C18, 3μM, 33 x 4.6, 3.5 ml/min 100 - 100% 50 mM ammonium acetate to acetonitrile in 4.5 minutes, C-^H IN ^ (581.2), 95%.

Example 211: Trans-7-[4-(N-tert-butoxycarbonyl-lS, 4S-2,5-diaza[2.2.1]heptanyl) cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine (183 mg, 20%)
Η NMR (d6 DMSO, 400 MHz) : δ 8.13 (IH, s), 7.39-7.47 (5H, m), 7.15-7.17 (IH, m), 7.07-7.11 (4H,m), 6.10 (2H, bs), 4.62 (IH, m), 4.1-4.2 (IH, m), 3.71 (IH, bs), 3.03 (2H, m), 2.35 (2H, m), 1.93-2.01 (6H, m), 1.60-1.68 (2H, m), 1.40 (9H, s). HPLC/MS Perkin Elmer Pecosphere C18, 3μM, 33 x 4.6, 3.5 ml/min 100 -100% 50 mM ammonium acetate to acetonitrile in 4.5 minutes, C30H36N6O (581.2), 99%.

Example 212: Cis-Nl -{4-[4-amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl] cyclohexyl}-Nl,N2,N2-trimethyl-l,2-ethanaediamine trimaleate salt
Trans-Nl - {4-[4-__mino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl-l,2-ethanaediamine trimaleate salt

A mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidine-7-yl]-l-cyclohexanone (1.0 g, 2.51 mmol), N,N,N'-trimethylethylenediamine (0.77 g, 7.54 mmol) and acetic acid (0.45 g, 7.54 mmol) in 1,2-dichloroethane (50 ml) was stined at ambient temperature under an atmosphere of nifrogen for 30 minutes. Sodium triacetoxyborohydride (0.69 g, 3.26 mmol) was added and the mixture stined at ambient temperature for 18 hours. Water (20 ml) and sodium bicarbonate (1.26 g, 15.1 mmol) were added, the mixture was stined for one hour, filtered through a pad of celite and the pad was washed with dichloromethane (75 ml). The filtrate was transfened to a separatory funnel and the layers were separated. The organic layer was dried over magnesium sulfate, filtered and the filtrate evaporated under reduced pressure. The cis and trans isomers were purified by flash
chromatography on silica gel using dichloromethane/methanol (7:3) as an eluent to give cis-Nl-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl- 1,2-ethanaediamine (0.442 g) and trans-Nl-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl- 1,2-ethanaediamine (0.336 g). The cis-Nl - {4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl-1,2-ethanaediamine (0.44 g, 0.909 mmol) was dissolved in warm ethyl acetate (100 ml) then maleic acid (0.32 g, 2.73 mmol) in ethyl acetate (30 ml) was added. The resulting salt formed an oily residue on the bottom and sides ofthe flask. The supernatant was poured off and the residue was dissolved in water and lyophilized to give cis-Nl-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl-l,2-ethanaediamine trimaleate salt (0.55 g): lH NMR (DMSO-d6, 400MHz) δ 8.22 (s, IH), 7.41-7.50 (m, 5H), 7.08-7.19 (m, 5H), 6.5 (bs, 2H), 6.15 (s, 6H), 4.78 (m, IH), 3.28 (m, 2H), 3.00 (m, 2H), 2.80 (m, IH), 2.79 (s, 6H), 2.50 (s, 3H), 2.19 (m, 2H), 1.99 (m, 2H), 1.78 (m, 4H); RP-HPLC (Hypersil CPS, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over 1 Omin, lml/min) t.=9.27 min; MS : MH+ 485.

frans-Nl - {4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl-l,2-ethanaediamine trimaleate salt was prepared from the free base in the same manner: lH NMR (DMSO-d6, 400MHz) δ 8.20 (s, IH), 7.41-7.48 (m, 5H), 7.08-7.19 (m, 5H), 6.45 (bs, 2H), 6.15 (s, 6H), 4.62 (m, IH), 2.9-3.3 (m, 5H), 2.74 (s, 6H), 2.56 (s, 3H), 1.9-2.2 (m, 6H), 1.73 (m, 2H); RP-HPLC (Hypersil CPS, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min) t-=8.17 min; MS: MH+ 485.

The following compounds were made in a similar manner to cis-Nl -{4-[4- ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}- Nl ,N2,N2-trimethyl- 1 ,2-ethanaediamine

Example 214: Cis-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)- 7H-pvnolo[2,3-d]pyrimidin-4-amine: Η NMR (d6 DMSO, 400 MHz): δ 8.13 (IH, s), 7.39-7.50 (4H, m), 7.28 (IH, s), 7.07-7.16 (5H, m), 6.08 (2H, bs), 4.67 (IH, m), 2.49-2.67 (9H, m), 2.06-2.16 (5H, m), 1.70-1.72 (2H, m), 1.53-1.59 (2H, m), 0.97 (d, J = 6.5 Hz, 6H). Mass spec. C3IH3gN6O (511.2). HPLC: (Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100%) acetonitrile-O.lN ammonium acetate over 10 min, 1 ml/min) t-=7.817 min., 99% TLC: Rf in 90% CH2Cl2/MeOH = 0.30 (UN visible).

Example 215: Trans-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)- 7H-pynolo[2,3-d]pyrimidin-4-amine: Η ΝMR (d6 DMSO, 400 MHz): δ 8.13 (IH, s), 740-7.47 (5H, m), 7.08-7.18 (5H, m), 6.08 (2H, bs), 4.53 (IH, m), 2.45-2.55 (9H, • m), 2.17-2.20 (IH, m), 1.86-1.96 (6H, m), 1.44-1.49 (2H, ), 0.97 (d, J = 5.5 Hz,

6H). Mass spec. C31H38Ν6O (511.2). HPLC: (Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100%) acetonitrile-O.lN ammonium acetate over 10 min, 1 ml/min) V=7.367 min., 91% TLC: Rf in 90% CH2Cl2/MeOH = 0.21 (UN visible).

Example 216: Cis-7- {4-[4-(2-methoxyethyl)piperazino]cyclohexyl} -5-(4- ρhenoxyphenyl)-7H-ρynolo[2,3-d]pyrimidin-4-amine: Η ΝMR (d6 DMSO, 400 MHz): δ 8.13 (IH, s), 7.39-7.50 (4H, m), 7.27 (IH, s), 7.07-7.11 (5H, m), 6.09 (2H, bs), 4.68 (IH, m), 3.42 (2H, t, J = 5.9 Hz), 3.22 (3H, s), 2.43-2.55 (9H, m), 2.03-2.16 (6H, m), 1.60-1.71 (2H, m), 1.52-1.59 (2H, m). Mass spec. C31H38Ν6O2 (527.2). HPLC: (Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100% acetonitrile-O.lN ammonium acetate over 10 min, 1 m^m ) ^=7.317 min, 95%> TLC: Rf in 90%> CH2Cl2/MeOH = 0.22 (UV visible).

Example 217: Trans-7-{4-[4-(2-methoxyethyl)piperazino]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidm-4-amine: *H NMR (d6 DMSO, 400 MHz): δ 8.13 (IH, s), 7.39-7.47 (5H, m), 7.07-7.16 (5H, m), 6.09 (2H, bs), 4.55 (IH, m), 3.36-3.42 (2H, m), 3.23 (3H, s), 2.33-2.55 (11H, m), 1.90-1.96 (6H, m), 1.44-1.47 (2H, m). Mass spec. C31H38N6O2 (527.2). HPLC: (Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100%) acetonitrile-O.lN ammonium acetate over 10 min, 1 ml/min) t=7.200 min, 99% TLC: Rf in 90% CH2Cl2/MeOH = 0.31 (UV visible).

Example 218: Cis-7-[-4-(4-ethylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-4-amine
Η NMR (d6 DMSO, 400 MHz) : δ 8.23 (IH, s), 7.41-7.49 (4H, m), 7.07-7.17 (6H,m), 6.57 (2H, bs), 6.20 (5H, s), 4.77 (IH, m), 2.04-2.13 (8H, m), 1.62-1.77 (5H, m), 1.21 (3H, t). HPLC (Waters delta pack C 18, 150 x 3.9 mm; 5 - 95% acetonitrile-0.1 M ammonium acetate over 30min, lml/min ) tr=13.851, 100%>.

trans-7-[4-(4-ethylρiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine
»H NMR (d6 DMSO, 400 MHz) : δ 8.19 (lH,s), 7.40-7.47 (4H, m), 7.19

(IH, ), 7.08-7.19 (5H,m), 6.40 (2H, bs), 6.18 (6H, s), 4.95 (IH, m), 3.17 (2H, bs), 2.98 (2H, bs), 2.69 (2H, bs), 1.94-2.01 (8H, m), 1.54-1.57 (2H, d, J =7.5 Hz), 1.17 (3H, t). HPLC (Waters delta pack C 18, 150 x 3.9 mm; 5 - 95% acetonitrile-0.1 M ammomum acetate over 30min, lml/min ) tr=13.701, 96%>.

The following compounds were prepared as salts in a similar manner to that offrans-Nl-{4-[4-amino-5-(4-phenoxyρhenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-Nl,N2,N2-trimethyl- 1,2-ethanaediamine trimaleate salt:

Example 219: Cis-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tris maleate: Η NMR (d6 DMSO, 400 MHz): δ 8.23 (IH, s), 7.40-7.49 (5H, m), 7.07-7.19 (5H, m), 6.55 (2H, bs), 6.16 (6H, s), 4.74 (IH, m), 3.26 (6H, bs), 2.04-2.49 (13H, m), 1.63-1.75 (5H, m), 1.25 (d, J = 6.6 Hz, 6H). Mass spec. C31H3gN6O (511.1). HPLC: (Hypersil HS Cl 8, 5μm, 254 nm, 250 x 4.6 mm; 25-100%) acetonitrile-O.lN ammonium acetate over 10 min, 1 ml/min) t-=7.967 min, 99%

Example 220: Trans-7-[4-(4-isopropylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tris maleate: Η NMR (d6 DMSO, 400 MHz): δ 8.20 (IH, s), 7.40-7.65 (5H, m), 7.08-7.19 (5H, m), 6.46 (2H, bs), 6.14 (6H, s), 4.60 (IH, m), 2.50-3.45 (17H, m), 1.95-2.02 (5H, m), 1.56-1.59 (2H, m), 1.20 (d, J = 6.5 Hz, 6H). Mass spec. C31H38N6O (511.2). HPLC: (Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100%) acetonitrile-O.lN ammonium acetate over 10 min, 1 ml/min) t_=7.733 min, 90%

Example 221 : Cis-7- {4-[4-(2-methoxyethy_)piperazino]cyclohexyl} -5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tris maleate: :H NMR (d6 DMSO, 400 MHz): δ 8.23 (IH, s), 7.41-7.49 (5H, m), 7.07-7.19 (5H, m), 6.55 (2H, bs), 6.16 (6H, s), 4.75 (IH, m), 3.62 (2H, m), 3.30 (3H, s), 3.17 (6H, bs), 2.50 (9H, m), 2.02-2.16 (5H, m), 1.74 (5H, m). Mass spec C31H38N6O2 (527.2). HPLC:
(Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100% acetonitrile-O.lN ammomum acetate over 10 min, 1 ml/min) t-=7.750 min, 99%>

Example 222: Trans-7-{4-[4-(2-methoxyethyl)piperazino]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]ρyrimidin-4-amine tris maleate: :H NMR (d6 DMSO, 400 MHz): δ 8.21 (IH, s), 7.41-7.48 (5H, m), 7.08-7.17 (5H, m), 6.53 (2H, bs), 6.17 (6H, s), 4.61 (IH, m), 3.45 (3H, s), 2.50-3.56 (19H, m), 1.99-2.08 (6H, m), 1.64 (2H, m). Mass spec C31H38N6O2 (527.2). HPLC: (Hypersil HS Cl 8, 5μm, 254 nm, 250 x 4.6 mm; 25-100%» acetonitrile-O.lN ammonium acetate over 10 min, 1 ml/min) t-=7.383 min, 99%

Example 223: Cis-Nl-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-7-yl]cyclohexyl} -N2,N2-dimethyl- 1 ,2-ethanaediamine trimaleate salt Trans-Nl - {4-[4-anιino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-N2,N2-dimethyl- 1,2-ethanaediamine monomaleate salt
cis-Nl-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-N2,N2-dimethyl- 1,2-ethanaediamine trimaleate salt: :H NMR (DMSO-d6, 400MHz) δ 8.19 (s, IH), 7.40-7.49 (m, 5H), 7.08-7.19 (m, 5H), 6.35 (bs, 2H), 6.13 (s, 6H), 4.78 (m, IH), 3.15-3.45 (m, 5H), 2.74 (s, 6H), 1.8-2.25 (m, 8H); RP-HPLC (Hypersil CPS, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t=8.90 min; MS: MH+ 471.

Example 224: trans-Nl-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-N2,N2-dimethyl-l,2-ethanaediamine monomaleate salt: Η NMR (DMSO-d6, 400MHz) δ 9.5 (bs, IH), 8.26 (s, IH), 7.41-7.55 (m, 5H), 7.08-7.19 (m, 5H), 6.7 (bs, 2H), 6.16 (s, 2H), 4.63 (m, IH), 3.12-3.55 (m, 5H), 2.85 (s, 3H), 2.27 (m, 2H), 1.99-2.05 (m, 4H), 1.67-1.75 (m, 2H); RP-HPLC (Hypersil CPS, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml min ) t-=8.6 min; MS: MH1" 471.

Example 225: Cis-7-(4-{[3-(lH-l-imidazolyl)propyl]amino}cyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate salt
Trans-7-(4-{[3-(lH-l-imidazolyl)propyl]amino}cyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dimaleate salt

Example 227: cis-7-(4-{[3-(lH-l-imidazolyl)propyl]amino}cyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate salt: :H NMR (DMSO-d6, 400MHz) δ 8.78 (bs, IH), 8.48 (bs, 2H), 8.18 (s, IH), 7.66 (s, IH), 7.55 (s, IH), 7.41-7.49 (m, 5H), 7.08-7.19 (m, 5H), 6.33 (bs, 2H), 6.12 (s, 6H), 4.78 (m,

IH), 4.27 (t, 2H), 2.99 (m, 3H), 1.8-2.25 (m, 10 H); RP-HPLC (Hypersil CPS, 5 μm,

100A, 250 x 4.6 mm; 25-100%) acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t_=9.07 min; MS: MH* 508.

Example 228: frans-7-(4-{[3-(lH-l-imidazolyl)propyl]amino}cyclohexyl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dimaleate salt: lH NMR

(DMSO-d6, 400MHz) δ 8.76 (bs, IH), 8.51 (bs, 2H), 8.18 (s, IH), 7.66 (s, IH), 7.55

(s, IH), 7.40-7.47 (m, 5H), 7.08-7.21 (m, 5H), 6.3 (bs, 2H), 6.11 (s, 4H), 4.60 ( , IH), 4.26 (t, 2H), 3.14 (m, IH), 2.97 (m, 2H), 1.9-2.25 (m, 8H), 1.53-1.61 (m, 2H);

RP-HPLC (Hypersil CPS, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammomum acetate over lOmin, lml/min ) ^=8.72 min; MS: MH1" 508.

Example 229: Cis-7-[4-(dimethylamino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dimaleate salt
Η NMR (DMSO-d6, 400MHz) δ 9.06 (bs, IH), 8.2 (s, IH), 7.41-7.50 (m, 5H), 7.08-7.19 (m, 5H), 6.4 (bs, 2H), 6.13 (s, 4H), 4.83 (m, IH), 3.34 (m, IH), 2.88 (s, 6H), 2.10-2.17 ( , 4H), 1.88-1.99 (m, 4H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25-100%) acetonitrile-0.1 M ammonium acetate over lOmin, lml mi ) t=7.38 min; MS: MH+ 428.

Example 230: Trans-5-(4-phenoxyphenyl)-7-(4-piperidinocyclohexyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dimaleate salt
Η NMR (DMSO-d6, 400MHz) δ 8.92 (bs, IH), 8.18 (s, IH), 7.4-7.5 (m, 5H), 7.08-7.19 (m, 5H), 6.3 (bs, 2H), 6.13 (s, 4H), 4.63 (m, IH), 3.15-3.5 (m, 3H), 2.9-3.1 (m, 2H), 1.16-2.18 (m, 14H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25-100%) acetonitrile-0.1 M ammomum acetate over lOmin, lml/min ) L=7.98 min; MS: MH+ 468. Trans-5-(4-phenoxyphenyl)-7-(4-tefrahydro-lH-l-pynolylcyclohexyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dimaleate salt Η MR (DMSO-d6, 400MHz) δ 9.54 (bs, IH), 8.18 (s, IH), 7.40-7.47 (m, 5H), 7.08-7.18 (m, 5H), 6.3 (bs, IH), 6.12 (s, 4H), 4.63 (m, IH), 3.1-3.55 (m, 5H), 2.24 (m, 2H), 2.00 (m, 6H), 1.86 (m, 2H), 1.67 (m, 2H); RP-HPLC (Hypersil HS C-18, 5 μm, lOOA, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) ^=7.82 min; MS: MH+ 454.

Example 231 : Cis-7-[4-(4-methyl-l ,4-diazepan-l-yl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dihydrochloride salt
Trans-7-[4-(4-methyl-l,4-diazepan-l-yl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dihydrochloride salt
cis-7-[4-(4-methyl-l,4-diazepan-l-yl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dihydrochloride salt: :H NMR (DMSO-d6,
400MHz) δ 11.7 (d, IH), 11.38 (d, IH), 8.57 (s, IH), 8.34 (d, IH), 7.42-7.51 (m, 4H), 7.03-7.20 (m, 5H), 4.93 (m, IH), 4.7 (bs, 2H), 3.4-3.99 (m, 9H), 2.8 (s, 3H), 1.86-2.57 (10 H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25- 100%) acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t =7.67 min; MS: MH+ 497.
trans-7- [4-(4-methyl- 1 ,4-diazepan- 1 -yl)cyclohexyl] -5 -(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine dihydrochloride salt: *H NMR (DMSO-d6,
400MHz) δ 11.94 (d, IH), 11.52 (d, IH), 8.56 (s, IH), 7.8 (s, IH), 7.42-7.51 (m, 4H), 7.10-7.20 (m, 5H), 4.76 (IH, m)< 3.2-4.0 (m, 9H), 2.80 (s, 3H), 1.78-2.4 (m, 10H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t.=7.42 min; MS: MH+ 497.

Example 232: Cis-5-(4-phenoxyphenyl)-7-(4-piperazinocyclohexyl)-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate salt
Trans-5-(4-phenoxyphenyl)-7-(4-piperazinocyclohexyl)-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate salt a) cis and trans-tert-butyl 4-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3- d]pyrimidin-7-yl]cyclohexyl} - 1 -piperazinecarboxylate

Example 233: cis-tert-butyl 4-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-l -piperazinecarboxylate: lH NMR (DMSO-d6,

400MHz) δ 8.14 (s, IH), 7.3-7.5 (m, 6H), 7.07-7.16 (m, 5H), 6.1 (bs, 2H), 4.69 (m, IH), 3.2-3.4 (4H, m), 2.38 (m, 4H), 2.0-2.25 (m, 5H), 1.5-1.8 (m, 4H), 1.41 (s, 9H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t=13.60 min.

frans-tert-butyl 4-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-l-piperazinecarboxylate: Η NMR (DMSO-d6, 400MHz) δ 8.13 (s, IH), 7.40-7.47 (m, 6H), 7.08-7.16 (m, 5H), 6.1 (bs, 2H), 4.55 (m, IH), 3.34 (m, 4H), 2.35-2.51 (m, 3H), 1.89-1.99 (m, 6H), 1.38-1.49 (m, 4H), 1.39 (s, 9H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOmin, lml/min ) t-=10.40 min.

b) Cis-5-(4-phenoxyphenyl)-7-(4-piperazinocyclohexyl)-7H-pyπolo[2,3- d]pyrimidin-4-amine trimaleate salt
The cis-tert-butyl 4- {4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-l -piperazinecarboxylate (1.85 g, 3.27 mmol) was treated with a 20% trifluoroacetic acid/dichloromethane solution (60 ml) and stined for 30 minutes at ambient temperature. The solvents were removed under reduced pressure then the residue was partitioned between dichloromethane (200 ml) and aqueous saturated sodium bicarbonate solution (30 ml). The organic solution was dried over magnesium sulfate, filtered and the filtrate evaporated to a residue (1.55 g). A portion of this material (1.0 g, 2.15 mmol) was dissolved in warm ethyl acetate (220 ml) then treated with maleic acid (0.75 g, 0.44 mmol) in warm ethyl acetate (75 ml). The mixture was cooled to ambient temperature then the solid was collected by filtration and dried to give Cis-5-(4-phenoxyphenyl)-7-(4- piperazinocyclohexyl)-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate salt (1.15 g) as a white solid: Η NMR (DMSO-d6, 400MHz) δ 8.5 (bs, IH), 8.23 (s, IH), 7.41-7.51 (m, 5H), 7.08-7.19 (m, 5H), 6.65 (bs, 2H), 6.16 (s, 6H), 4.74 (m, IH), 1.16-3.2 (m, 17H); RP-HPLC (Hypersil CPS, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammomum acetate over lOmin, lml min ) t-=8.63 min; MS: MET 469.

c) trans-5-(4-phenoxyphenyl)-7-(4-piperazinocyclohexyl)-7H-pynolo[2,3- d]pyrimidin-4-amine trimaleate salt
Η NMR (DMSO-d6, 400MHz) δ 8.22 (s, IH), 7.41-7.51 (m, 5H), 7.08-7.19

(m, 5H), 6.6 (bs, 2H), 6.16 (s, 6H), 4.58 (m, IH), 1.4-3.2 (m, 17 H); RP-HPLC (Hypersil HS C-18, 5 μm, 100A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammonium acetate over lOrnin, lml min ) ^=8.08 min; MS: MH+ 469.

Example 234: 7-[3-(4-methylpiperazino)cyclopentyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-maleate
3-[4-amino-5-(4-phenoxyρhenyl)-7H-ρynolo[2,3-d]pyrimidin-7-yl]cyclopentan-l-ol (2.14 g, 0.0055 mol) in 1 1 dichloromethane was stined with 12 g active manganese dioxide for 5 hours, filtered and fresh manganese dioxide (8 g) added to the filtrate. After stirring for a further 17 hours, the mixture was filtered and used directly. HPLC/MS showed starting material and 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l-cyclopentanone 62.7%> X_ 4.38 minutes. The dichloromethane solution was stined with 1.0 g N-methylpiperazine (0.01 mol) and acetic acid (0.6 g, 0.01 mol) for 15 minutes then sodium
triacetoxyborohydride (0.89 g, 0.0042 mol) was added. After 2 hours 1.0 g N-methylpiperazine, 0.6 g acetic acid and 0.89 g sodium triacetoxyborohydride was added and the mixture stined for 17 hours. Further addition of 2.0 g N-methylpiperazine, 1.2 g acetic acid and 1.2 g sodium triacetoxyborohydride and stirring for 3 days gave a mixture which was evaporated under reduced pressure. The residue was treated with water (200 ml) and 6M - hydrochloric acid (50 ml) then washed with ethyl acetate (discarded) and basified with excess aqueous ammonia. The mixture was extracted with ethyl acetate and the extract dried (sodium sulphate) then purified by flash chromatography in 9:1 ethyl acetate : ethanol to remove impurities followed by 8:1:1 ethyl acetate : ethanol : triethylamine to elute the product. Solvent was removed under reduced pressure, the residue dissolved in ethyl acetate and treated with a solution of maleic acid in ethyl acetate giving 7-[3-(4-methylpiperazino) cyclopentyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-maleate (444395) as a 1.4 solvate with ethyl acetate after drying at 80° C under reduced pressure (0.95 g, 0.001 mol) m.pt. 168-170° C (decomposes).

Example 235: [4-(4-amino-7-cyclopentyl-7H-pynolo[2,3-d]pyrimidin-5-yl)phenyl](phenyl)-methanol, Sodium borohydride ( 0.052 g, 0.0013 mol) was added to a solution of [4-(4-amino-7-cyclopentyl-7H-pynolo[2,3-d]pyrimidin-5-yl)phenyl](phenyl)mexhanone ( 0.1 g, 0.00026 mol) in tetrahydrofuran ( 4 mL) followed by the addition of Amberlyst-15 HΛ The mixture was stined at ambient temperature under an atmosphere of nitrogen for 15 min, filtered through a celite pad and the solvent removed under reduced pressure. The residue was purified by preparative RP-HPLC (Rainin, Hypersil C18, 8 μm, 100 A, 25 cm; 5%-85% acetonitrile - 0.1 %> ammonium acetate over 20 min, 21 mL/min) to yield [4-(4-amino-7-cyclopentyl-7H-pynolo[2,3-d]pyrimidin-5-yl)phenyl](phenyl)methanol (0.005 g, 0.000013 mol):
XH NMR ( DMSO-d6>400MHz) δ 8.12 (s, IH), 7.31 (m, 10H), 6.01 ( br, 2H), 5.91 (d, IH), 5.75 (d, IH), 5.06 (m, IH), 2.10(br, 2H), 1.88 ( br, 4 H), 1.67 (br, 2H) RP-HPLC( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammomum acetate over 20 min, lmL / min) R_ 16.74 min. MH+ 385

Example 236: Trans-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-maleate
trans-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine (1.30 g, 0.0027 mol) in 300 ml warm ethyl acetate was treated with a solution of maleic acid (0.94 g, 0.0081 mol) in 100 ml ethyl acetate and allowed to cool. The colourless solid was collected, washed with ethyl acetate and dried to constant weight at 90° C / 3 mbar giving 1.85 g (0.0022 mol) of trans-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrinfidin-4-amine tri-maleate solvated with 0.18 mol ethyl acetate m.p. 189° C (decomposes).

Example 237: trans-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-hydrochloride
trans-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine (0.36 g, 0.00075 mol) in 25 ml warm isopropanol was treated with a solution of 0.225 ml 12M hydrochloric acid (0.0027 mol) in 2 ml isopropanol and the suspension heated briefly to boiling then volatile material was removed under reduced pressure. The resulting colourless solid was dried to constant weight at 84° C / 5 mbar giving the trans-7-[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-hydrochloride (444626) solvated with 1 mol water and 0.25 mol isopropanol (0.25 g, 0.0004 mol) m.p. 304-306° C(dec).

Example 238: cis-7 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-maleate salt
cis-7 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-4-amine (1.45 g, 0.0030 mol) in ethyl acetate with 1.05 g (0.0091 mol) maleic acid giving colourless solid after drying to constant weight at 90° C / 3 mbar. 2.15 g cis-7 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-maleate salt solvated with 0.14 mol ethyl acetate and 0.5 mol water (0.0025 mol) obtained m.p. 186 (dec).

Example 239: cis-7 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-hydrochloride cis-7 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine 0.80 g (0.0017 mol) in isopropanol was treated with 0.5 ml 12M hydrochloric acid (0.006 mol). The resulting solid was filtered to give cis-7 -[3-(4-methylpiperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amine tri-hydrochloride as a hygroscopic solid until dried at 80° C / 3 mbar to constant weight. (0.75 g, 0.0011 mol) m.p. 224.5-226.5 (dec).

Example 240: Trans-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazmo)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate
A mixture of 3-phenoxytoluene (2.5 g, 0.0136 mol) and N-bromosucci mide (2.54 g, 0.0142 mol) was stined in acetonitrile (20 mL) for 2.5 hours under an atmosphere of nifrogen. The solvent was removed under reduced pressure. Carbon tetrachloride was added to the residue and the resulting solid was removed by filtration. The filfrate was concenfrated to yield 4-bromo-3-methylphenyl phenyl ether as yellow oil (3.5 g, 0.0133 mol):
Η NMR (Chloroform-d, 400 MHz) δ 7.45 (d, IH), 7.33 (m, 2H), 7.12 (t, IH), 7.00 (d, 2H), 6.89 (s, IH), 6.71 (d, IH), 2.34 (s, 3H) RP-HPLC (Hypersil C18, 5μm, 250 x 4.6 mm; 25% - 100%> over 23 min with 0.1 M ammonium acetate, lmL/min) Rj 14.72 min.
A mixture of 4-bromo-3-methylphenyl phenyl ether (1.7 g, 0.00646 mol), diboron pinacol ester (2.0 g, 0.00775 mol), [l, -bis(diphenylphosphino)fenocene] dichloropalladium(II) complex with dichloromethane (1:1) (0.16 g, 0.00019 mol) and potassium acetate (1.9 g, 0.01938 mol) in N,N-dimethylformamide (65 mL) was heated at 80 °C under an atmosphere of nitrogen for 22 hours. The mixture was allowed to cool to ambient temperature and the solvent was removed under reduced pressure. Dichloromethane was added to the residue and the resulting solid was removed by filtration through a pad of celite. The filtrate was concentrated into black mixture, which was purified by flash chromatography on silica using ethyl acetate/n-heptane (3:97) as mobile phase to yield 3-methyl-4-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)phenyl phenyl ether (1.05 g, 0.00338 mol):
Η NMR (Chloroform-d, 400 MHz) δ 7.73 (d, IH), 7.33(m, 2H), 7.08 (t, IH), 7.01 (d, 2H), 6.79 (d, 2H), 2.51 (s, 3H), 1.34 (s, 12H) TLC (ethyl acetate / n-heptane = 3 : 97) Rf 0.28
A mixture of 4-chloro-7-(l ,4-dioxaspiro[4.5]dec-8-yl)-5-iodo-7H-pynolo[2,3-d]pyrimidine (20 g, 47.7 mmol) and 6 N HCl(aq) (60 mL, 360 mmol) in tefrahydrofuran (120 mL) and acetone (600 mL) was stined at ambient temperature under an atmosphere of nifrogen for 17 hours. The solvent was removed under reduced pressure and 6NHCl(aq) (20 mL), tefrahydrofuran (60 mL), and acetone (300 mL) were added to the mixture. The mixture was stined at ambient
temperature under an atmosphere of nitrogen for 4.5 hour. The solvent was removed under reduced pressure and the yellow colored residue was washed with water to yield 4-(4-chloro-5-iodo-7H-pyπolo[2,3-d]pyrimidin-7-yl)-l-cyclohexanone (12.3 g, 32.7 mmol). RP-HPLC (Hypersil C18, 5μm, 250 x 4.6 mm; 25% - 100% over 15 min with 0.05 M ammonium acetate, lmL/min) ^ 10.20 min.
A mixture of 4-(4-chloro-5-iodo-7H-pyπolo[2,3-d]pyrirnidin-7-yl)-l-cyclohexanone (5.6 g, 14.9 mmol), N-methylpiperazine (3.3 mL, 29.8 mmol), acetic acid (2.6 mL, 44.7 mmol), and trimethylorthoformate (9.9 mL, 89.4 mmol) in dichloroethane (100 mL) was stined at ambient temperature under an atmosphere of nitrogen for 1 hr. Sodium triacetoxyborohydride (14.2 g, 67.05 mmol) was added into the mixture and stined at ambient temperature under an atmosphere of nitrogen for 18 hours. The solvent was removed under reduced pressure. The residue was partitioned between saturated aqueous sodium bicarbonate solution and ethyl acetate. The water phase was further extracted with ethyl acetate and the combined organic extracts were dried over sodium sulfate. The solvent was removed under reduced pressure and the residue was purified by flash chromatography on silica gel using triethylamine/ dichloromethane (2:98) followed by
melhanol triethylamine/dichloromethane (2:3:95) as mobile phase to yield trans-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidine (1.7 g, 3.7 mmol). Η NMR (DMSO-d6, 400 MHz) 8.63(s, IH), 8.12 (s, IH), 4.63 (br, IH), 2.15 (s, 3H), 1.94 (br, 6H), 1.45 (br, 2H) RP-HPLC (Hypersil C18, 5μm, 250 x 4.6 mm; 25% - 100%) over 15 min with 0.05 M ammomum acetate, lmL/min) Rt 6.17 min.

Trans-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidine (0.89 g, 1.9 mmol) in concentrated ammonium hydroxide (40 mL) and dioxane (40 mL) was heated at 120 °C in a pressure vessel for 18 hours. The mixture was allowed to cool to ambient temperature and the solvent was removed under reduced pressure. The residue was partitioned between saturated aqueous sodium bicarbonate solution and ethyl acetate. The water phase was further extracted with ethyl acetate and the combined organic exfracts were washed with brine and dried over sodium sulfate. The solvent was removed under reduced pressure to yield trans-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (0.35 g, 0.8 mmol). RP-HPLC (Hypersil C18, 5μm, 250 x 4.6 mm; 25%> - 100%> over 15 min with 0.1 M ammonium acetate, lmL/min) R_ 4.01 min. MS: MH+ 441

A mixture of frans-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrimidin-4-amine (0.347 g, 0.000788 mol), 3-methyl-4-(4,4,5,5-teframethyl-l,3,2-dioxaborolan-2-yl)phenyl phenyl ether (0.27 g, 0.000867 mol), tetrakis(friphenyl-phosphine)palladium(0) (0.054 g, 0.000047 mmol), and sodium carbonate (0.209 g, 0.00197 mol) in N,N-dimethylformamide (15 mL) and water (10 mL) was heated at 80 °C under an atmosphere of nitrogen for 16 hours. The mixture was allowed to cool to ambient temperature and the solvent removed under reduced pressure. The residue was partitioned between saturated aqueous sodium
bicarbonate solution and ethyl acetate. The water phase was further extracted with ethyl acetate and the combined organic extracts were dried over sodium sulfate. The solvent was removed under reduced pressure and the residue was purified by flash chromatography on silica gel using triethylamine/dichloromethane (5:95) followed by methanol/ triethylamine/ dichloromethane (3:5:92) as mobile phase to yield trans-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H- ρynolo[2,3-d]ρyriιnidin-4-amine (0.376 g, 0.000757 mol). Trans-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)-cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (0.376 g, 0.000757 mol) was dissolved in refluxing ethanol (10 mL) and a preheated solution of maleic acid (0.264 g, 0.00227 mol) in ethanol (5 mL) was added. The mixture was refluxed for 15 minutes, cooled to ambient temperature and the precipitate collected by filfration, washed with cool ethanol and dried to give frans-5-(2-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)-cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine trimaleate (0.153 g, 0.000181 mol): Η NMR (DMSO-d6, 400 MHz) 8.22 (s, IH), 7.42 (m, 3H), 7.25 (d, IH), 7.17 (t, IH), 7.09 (d, 2H), 7.02 (s, IH), 6.89 (d, IH), 6.16 (s, 6H), 4.58 (m, IH), 3.3 (br, 9H), 2.68 (s, 3H), 2.22 (s, 3H), 2.01 (br, 6H), 1.57 (br, 2H) RP-HPLC (Hypersil C18, 5μm, 250 x 4.6 mm; 25%> - 100%> over 23 min with 0.1 M ammonium acetate, lmL/min) R, 7.30 min. MS: MH+ 497

Example 241: 3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl 2-aminoacetate hydrochloride
A mixture of 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l-cyclopentanol (50 mg, 0.129 mmol), 2-[(tert-butoxycarbonyl)amino]acetic acid (34 mg, 0.194 mmol), l-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (31 mg, 0.155 mmol) and 4-(dimethylamino)pyridine (16 mg, 0.129 mmol) in DMF (1 mL) was stined under nitrogen for 24 hours. The mixture was pour onto ice-water. The aqueous layer was extracted with ethyl acetate three times. The combined organic layer was washed with brine, dried over MgSO4, filtered and evaporated. The residue was purified by flash column chromatography using ethyl acetate as mobile phase to give 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl 2-[(tert-butoxycarbonyl)amino]acetate (39 mg, 0.072 mmol). HPLC: tr=19.22 min. (Delta-Pack, C-18, 5um, 300A, 3.9x150 mm; 5-85% acetonitrile-0.1 M ammonium acetate over 20 min, lml/min )
3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl 2- [(tert-butoxycarbonyl)amino] acetate (39 mg, 0.072 mmol) was dissolved in ethyl acetate (2.5 mL). Hydrochloride gas was bubbled through the solution for 3 minutes. The reaction mixture was stined for additional 30 minutes. Ether was added and the precipatate was collected through filtration under nitrogen to give 3-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl 2-aminoacetate hydrochloride (39 mg) as white solid. IH NMR (DMSO-d6) δ 2.20 (m, 5H), 2.67 (m, IH), 3.83 (s, 2H), 5.25 (m, IH), 5.31 (m, IH), 7.14 (m, 2H), 7.43, (m, IH), 7.50 (m, IH), 7.68 (m, IH), 8.26 (bs, 2H), 8.40 (s, IH). LC/MS: MH+=444, tr=2.25 min. (Pecospher, 3C-18, 3um, 4.6x33 mm; 0-100% acetonitrile-0.1 M ammonium acetate over 5 min, 3.5 ml/min )

Example 242 : 3 - [4- Amino-5 -(4-phenoxyphenyl)-7H-pynolo [2,3 -d]pyrimidin-7-yl]cyclopentyl N-(2-moφholinoethyl)carbamate hydrochloride
4- Nifrochloroformate (12.5 mg, 0.062 mmol) in dichloromethane (1 mL) was cooled on an ice-water bath. 4-Methylmoφholine (7 uL, 0.062 mmol) was added slowly. After 20 minutes, the ice- water bath was removed and the reaction mixture was allowed to warm up to room temperature. 3-(4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl)-l-cyclopentanol (20 mg, 0.052 mmol) was added and the reaction mixture was stined for 4days. The reaction mixture was diluted with dichloromethane. The organic layer was washed with water, saturated sodium bicarbonate, brine, dried over MgSO4, filtered and evaporated to give a yellow solid. A solution ofthe yellow solid in dichloromethane (1 mL) was added to 2-moφholino-l-ethanamine (0.2 mL). After stirring at room temperature overnight, the reaction mixture was diluted with ethyl acetate. The organic layer was washed with water (3 times), brine, dried over MgSO4, filtered and evaporated. The crude product was purified by HPLC to give 3-[4-amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentyl N-(2-moφholinoethyl)carbamate (17 mg, 0.031 mmol). IH NMR (CDCl3-d) δ 2.08 (m, 4H), 2.43 (m, 7H), 2.73 (m, IH), 3.29 (m, 2H), 3.67, (m, 4H), 5.28 (m, 5H), 7.09 (m, 6H), 7.40 (m, 4H), 8.30 (s, IH). LC/MS: MH+=543, tr=2.13 min. (Pecospher, 3C-18, 3um, 4.6x33 mm; 0-100%> acetonitrile-0.1 M ammomum acetate over 5 min, 3.5 ml/min).
3-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yljcyclopentyl N-(2-moφholinoethyl)carbamate (10 mg, 0.0184 mmol) was dissolved in ethyl acetate (2.5 mL). Hydrochloride gas was bubbled through the solution for 3 minutes. The reaction mixture was stined for additional 10 minutes. The precipitate was collected through filtration under nitrogen to give 3-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclopentylN-(2-moφholinoethyl)carbamate hydrochloride as white solid. IH NMR (DMSO-d6) δ 1.99 (m, 4H), 2.55(m, 2H), 3.32 (m, 12H), 5.08(m, 1/2H), 5.19 (m, 1/2H), 7.16 (m, 5H), 7.45, (m, 5H), 8.26 (s, IH). LC MS: MH+=543, tr=2.16 min. (Pecospher, 3C- 18, 3um, 4.6x33 mm; 0-100% acetonitrile-0.1 M ammonium acetate over 5 min, 3.5 ml/min).

Example 243 : 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl] cyclohexanol
Sodium borohydride (500mg, 13 mmol) was added in one portion to a stined solution of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin- 7-yl]cyclohexan-l-one (780mg, 2.0 mmol) in methanol (500 mL), and the mixture stined under an atmosphere of nifrogen for 1 hour, then left to stand overnight. The solvent was removed under reduced pressure, and the residue partitioned between 2M aqueous sodium hydroxide solution (100 mL) and dichloromethane (100 mL). The organic layer was separated and the aqueous layer further extracted with dichloromethane (2 x 100 mL). The combined organic exfracts were washed with water (150 mL), dried over potassium carbonate, and purified by chromatography with a Biotage 40S column using ethyl acetate / triethylamine (98:2 to 95 : 5) and ethyl acetate / ethanol (95:5) as a mobile phase to yield 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexanol as a white solid (750mg, 1.9 mmol), melting point: 199-200 deg. C.LC/MS: Hypersil BDS cl8 (100 x 2.1 mm) 0.1M ammoniumacetate/acetonitrile, 10-100%) acetonitrile in 8 min.)MH+ 401 , t- = 4.12 minutes.

Example 244
Phenyl N-[4-(4-ammo-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)- 2-methoxyphenyl]carbamate
-(4- Amino-3 -methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo [2,3 -_f]pyrimidin-4-amine (100 mg, 0.294 mmol) was dissolved in dichloromethane (2 mL). Pyridine (2mL) was added followed by phenylchloroformate (44 uL, 0.353 mmol). After stirring for 3 hours, another 44 uL of phenylmethanesulfonyl chloride was added and the reaction mixture was stined overnight. The solvent was removed and the residue was purified by preparative LC/MS to give phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (52 mg, 0.113 mmol). 1Η ΝMR (CDCl3-_f) δ 2.09 (m, 4Η), 3.66 (m, 2H), 3.98 (s, 3H), 4.16 (m, 2H), 4.98 (m, IH), 5.24 (s, 2H), 7.09 (m ,3H), 7.23 (m, 4H), 7.41 (m, 2H), 7.62 (s, IH), 8.20(bd, J=7.80 Hz, IH), 8.33 (s, IH). LC/MS MH+=460.

Example 245
Tefrahydro-2H-4-pyranyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-rf]pyrimidin-5-yl)-2-methoxyphenyl]carbamate 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate
Tetrahydro-2H-4-pyranol (1.0 ml, 10.5 mmol) was mixed with 4-methylmoφholine (2.0 ml) in dichloromethane (20 mL). 4- Nifrochloroformate (1.98 g, 9.82 mmol) was added slowly to the reaction mixture. After stirring for 5 hours, the reaction mixture was diluted with dichloromethane. The organic layer was washed with water, 1.0 N ΗC1, saturated sodium bicarbonate, brine, dried over MgSO4, filtered and evaporated. The crude product was purified by flash column chromatography chromatography using ethyl acetate/heptane (4:1) as the mobile phase to give 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate (1.5 g, 5.62 mmol). 1Η NMR (CDCl3-_f) δ 1.87 (m, 2Η), 2.06 (m, 2H), 3.58 ( , 2H), 3.98 (m, 2H), 4.97 (m,lH), 7.40(d, J=9.0Hz, 2H), 8.30 (d, J=9.0Hz, 2H).

a) Tefrahydro-2H-4-pyranyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3--i]pyrimidin-4-amine (57 mg, 0.168 mmol) and 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate (90 mg, 0.336 mmol) was mixed in pyridine (1 mL). After stirring for 5 hours, another 90 mg of 4-nitrophenyl tetrahydro-2H-4-pyranyl carbonate was added and the reaction mixture was stined for 2 days. The reaction mixture was heated at 70°C for 2 hours. The solvent was removed and the residue was purified by preparative thin layer chromatography to give tetrahydro-2H-4-pyranyl N-[4-(4-annno-7-tefr_thydro-2H-4-pyranyl-7H-pynolo[2,3-.i]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.064 mmol). 1Η ΝMR (CDCl3-_ ) δ 1.78 (m, 4Η), 2.08 (m, 4H), 3.60 (m, 4H), 3.94 (s, 3H), 3.97 (m, 2H), 4.15 (m, 2H), 4.98 (m, 2H), 5.23 (s, 2H), 6.78 (s ,1H), 7.04 (s, IH), 7.07 (d, J=8.3 Hz, IH), 8.16(bd, J=7.90 Hz, IH), 8.33 (s, IH). LC/MS MH+=468.

Example 246
3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride

a) 4-Νitrophenyl (3-pyridylmethyl) carbonate
4- Nifrochloroformate (2.49 g, 12.3 mmol) in dichloromethane (20 mL) was cooled on an ice-water bath. 3-pyridylmethanol (1.0 mL, 10.3 mmol) and 4-methyhnoφholine (2.0 mL, 18.5 mmol) was added slowly. After 20 minutes, the ice-water bath was removed and the reaction mixture was allowed to warm up to room temperature. 30 minues later, ethyl acetate was added and the reaction mixture was filtered. The filtrate was washed with water, saturated sodium bicarbonate, brine, dried over MgSO4, filtered and evaporated to give a dark brown solid which was re-crystallized with ethyl acetate/heptane to give 4-nitrophenyl (3-pyridylmethyl) carbonate (1.52 g, 5.54 mmol).lΗ NMR (CDCl-cQ δ 7.38 (m, 3H), 7.79 (m, IH), 8.28 (d, J=9.09Hz, 2H), 8.65 (m, IH), 8.72 (s,lH).

b) 3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-c ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyrimidin-4-amine (25 mg, 0.074 mmol) was dissolved in dichloromethane (0.7 mL). Pyridine (0.7 mL) was added followed by 4-nitrophenyl (3-pyridylmethyl) carbonate (30 mg, 0.110 mmol). After heating at 100°C overnight, the solvent was removed and the residue was purified by preparative LC/MS to give 3-pyridylmethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-< ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (12 mg, 0.025 mmol). 1Η ΝMR (CDCX3-d) δ 2.08 (m, 4Η), 3.65 (m, 2H), 3.92 (s, 3H), 4.15 (m, 2H), 4.96 (m, IH), 5.26 (s, 2H), 5.54 (bs, 2H), 6.97 (s, IH), 7.04(s, IH), 7.08 (d, J=8.2Hz, IH), 7.35 (m, 2H), 7.79 (d,
J=7.8Hz, IH), 8.15 (m, IH), 8.29 (s, IH), 8.61 (s, IH), 8.71 (s, IH). LC/MS
MH+=475

b) 3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- _/]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride
3-Pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (12 mg, 0.025 mmol) was dissolved in ethyl acetate (2.0mL). 1 ,0Ν ΗC1 in ether (1 mL) was added slowly. The precipatate was collected through filtration under nitrogen to give 3-pyridylmethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-_ ]pyrimidin-5-yl)-2-methoxyphenyl] carbamate hydrochloride(13 mg, 0.25 mmol). 1Η MR (DMSO-t 6) δ 1.91 (m, 2Η), 2.17(m, 2H), 3.54 (m, 2H), 3.87 (s, 3H), 4.03 (m, 2H), 4.97(m, IH), 5.23 (s, 2H), 7.05 (d, J=8.2Hz, IH), 7.13 (s, IH), 7.51 (m, IH), 7.81 (d, J=8.2Hz, IH), 7.84 (s, IH), 7.95 (m, IH), 8.42 (s, IH), 8.60(s, IH), 8.71 (s, IH), 8.82 (s, IH). LC/MS MfT=475.

Example 247
2-Moφholinoethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-cT]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyrimidm-5-yl)-2-methoxyphenyl]carbamate (25 mg, 0.054 mmol) was mixed with 2-moφholino-l -ethanol (0.1 mL) in pyridine (0.7 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase ΗPLC to give 2-moφholinoethyl N-[4-(4-__mino-7-tefrahy(fro-2H-4-pyranyl-7H-pynolo[2,3-^pyrimidin-5-yl)-2-methoxyphenyl]carbamate (24 mg, 0.048mmol). The solid was dissolved in ethyl acetate (2 mL) and 1.0Ν ΗC1 in ether (0.2 mL) was added slowly. The precipitate was collected through filtration under nitrogen to give 2-moφholinoethyl N-[4-(4-anιino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_fIpyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride (24 mg, 0.045 mmol). 1Η ΝMR (DMSO-d6) δ 1.88(m, 2Η), 2.16(m, 2H), 3.55 (m, 8H), 3.90 (s, 3H), 4.03 (m, 4H), 4.49(m, 2H), 4.92 (m, IH), 7.07 (m, IH), 7.15 (s, IH), 7.65 (bs, 2H), 7. 84 (s, IH), 8.45 (s, IH), 8.75(s, IH) 10.95 (bs, IH). LC/MS MH+=497.

Example 248
(4-Bromo- 1 ,3-thiazol-5-yl)methyl N-[4-(4-amino-7-tefrahydro-2H-4-ρyranyl-7H-pynolo[2,3-cTlpyrimidin-5-yl)-2-methoxyphenyl]carbamate

a)2,4-Dibromo- 1 ,3-thiazole-5-carbaldehyde
l,3-Thiazolane-2,4-dione (3.52 g, 30 mmol) and phosphorus oxybromide (43 g, 150 mmol) were mixed with dimethyl formamide (2.56 mL, 34 mmol). The mixture was then heated at 75°C for 1 hours and at lOOoC for 5 hours. After cooled to room temperature, the mixture was added to ice-water (500ml) and the aqueous layer was extracted with dichloromethane. The combined organic layer was washed with saturated sodium bicarbonate, dried over MgSO4, filtered and evaporated to give a brown solid which was washed with petroleum ether. Evaporation of solvent gave 2,4-dibromo-l,3-thiazole-5-carbaldehyde (1.74 g, 6.42 mmol). IH NMR (CDCl3-c ) δ 9.90 (S, IH).

b) (2,4-Dibromo-l,3-thiazol-5-yl)methanol
2,4-Dibromo-l,3-thiazole-5-carbaldehyde (1.74 g, 6.42 mmol) was dissolved in methanol (70 ml) at 0°C. Sodium borohydride (0.244 g, 6.42 mmol) was added in small portions. The ice- water bath was removed 10 minutes later and the reaction mixture was stined at room temperature overnight. Solvent was removed and saturated ammomum chloride was added. 1.ON NaOH was added to adjust the pH to 10. The aqueous layer was exfracted with ethyl acetate. The combined organic layer was washed with brine, dried over MgSO4, filtered and evaporated. The residue was purified by flash column chromatogφhy to give (2,4-dibromo-l,3-thiazol-5-yl)methanol (0.946 g, 3.47 mmol). IH NMR (CDCl3-d) δ 2.11 (bs, IH), 4.79 (S, 2H).

c) (4-Bromo-l,3-thiazol-5-yl)methanol
(2,4-Dibromo-l,3-thiazol-5-yl)methanol (0.94 g, 3.44 mmol), sodium carbonate tri-hydrade (1.34 g) and palladium on carbon (10%>, 0.07g) were mixed in methanol (33 mL). The resulting mixture was hydrogenated at 60 psi for 2 days. The solid was filtered off through a pat of celite. The solvent was evaporated and the residue was purified by frash column chromatography to give (4-bromo-l,3-thiazol-5-yl)methanol (0.32 g, 2.78 mmol). IH NMR (CDCl3-_f) δ 2.29 (bs, IH), 4.86 (s, 2H), 8.72 (s, IH).

d) (4-Bromo- 1 ,3-thiazol-5-yl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-ρyranyl-7H-pyπolo[2,3-- ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate
Phenyl N-[4-(4-amino-7-tefrahydro-2H-4-pvranyl-7H-pynolo[2,3-_ ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (28 mg, 0.061 mmol) was mixed with (4-bromo-l,3-thiazol-5-yl)methanol (50 mg, 0.434 mmol) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give (4-bromo-l,3-thiazol-5-yl)methyl N- [4-(4-a rι_no-7-tefrahydro-2
yl)-2-methoxyphenyl]carbamate. IH ΝMR (CDCX-d) δ 2.07(m, 4H), 3.65 (m, 2H), 3.92 (s, 3H), 4.13 (m, 2H), 4.98 (m, IH), 5.35 (s, IH), 5.40(s, 2H), 6.97 (s, IH), 7.04 (s, IH), 7.09 (m, IH), 7.35 (s, IH), 8.17 (s, IH), 8.32 (s, IH), 8.78(s, IH). LC/MS MH+=481.

Example 249
Tefrahydro-3-furanyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3--dpyrimidm-5-yl)-2-methoxyphenyl]carbamate

Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-cr]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed with tetrahydro-3-furanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase PΗLC to give tetrahydro-3-furanyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (14 mg, 0.031mmol). 1Η MR (CDCl-rf) δ 2.07(m, 6Η), 3.66 (m, 2H), 3.96 (m, 7H), 4.13 (m, 2H), 4.98 (m, IH), 5.26 (s, 2H), 5.40(m, IH), 6.97 (s, IH), 7.04 (s, IH), 7.08 (d, J=8.2Hz, IH), 7.26 (s, IH), 8.30 (s, IH), 8.32 (s, IH). LC/MS MH+=455.

Examples 250
1 ,3-Dioxan-5-yl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- ^pyrimidin-5-yl)-2-methoxyphenyl]carbamate
l,3-Dioxolan-4-y_methyl N-(4-(4-__mmo-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3- _ ]pyrimidin-5-yl)-2-methoxyphenyl)carbamate
Phenyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3- _ ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed glycerol formal (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase PHLC to give tetrahydro-3-furanyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-_ ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (2 mg, 0.004mmol). 1Η ΝMR (CDCl-d δ 2.06(m, 4Η), 3.66 (m, 2H), 3.92 (m, 3H), 4.07 (m, 6H), 4.79 (m, IH), 4.83 (d, J=6.3Hz, IH), 4.96 (m, IH), 5.04(d, J=6.3Hz, IH), 6.15 (vbs, 2H), 6.96 (s, IH), 7.05 (m, 2H), 7.53 (s, IH), 8.15 (d, J=8.2Hz, IH), 8.22 (s, IH). LC/MS MH+=471 and l,3-dioxolan-4-ylmethylN-(4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyriι din-5-yl)-2-methoxyphenyl)carbamate(6.0mg, 0.013 mmol). 1Η ΝMR ( DCl-d) δ 2.06(m, 4Η), 3.66 (m, 2H), 3.75 (m, IH), 3.92 (m, 3H), 4.03 (m, IH), 4.13 (m, IH), 4.34 (m, 2H), 4.94 (s, IH), 4.97 (m, IH), 5.10(s, IH), 5.32 (bs, 2H), 6.97 (s, IH), 7.03 (m, 2H), 7.06 (d, J=8.2Hz, IH), 7.38(s, IH), 8.15 (d, J=7.9Hz, IH), 8.31 (s, lH). LC/MS MH+=471.

Example 251
2-Pyridylmethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride

Phenyl N-[4-(4-an_ino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed 2-pyridylmethanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give 2-pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-^pyrimidin-5-yl)-2-methoxyphenyl]carbamate (11 mg, 0.023 mmol). The solid was dissolved in ethyl acetate (2 mL) and 1.0Ν ΗC1 in ether (0.1 mL) was added slowly. The precipitate was collected through filfration under nifrogen to give 2-pyridylmethyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_ ]pyrimidin-5-yl)-2-methoxyphenyl]carbamate hydrochloride (12 mg, 0.023 mmol). 1Η ΝMR (DMSO-rf6) δ 1.92(m, 2Η), 2.16(m, 2H), 3.55 (m, 2H), 3.89 (s, 3H), 4.02 (m, 2H), 4.91 (m, IH), 5.23 (s, 2H), 7.05 (d, J=8.2Hz, IH), 7.14 (s, IH), 7.37 (m, IH), 7. 53 (d, J=7.8Hz, IH), 7.87 (m, 3H), 8.42(s, IH), 8.57 (d, J=4.2Hz, IH), 8.85 (s, IH). LC/MS MH+=475.

Example 252
4-PyridylmethylN-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_^]pyrimidin-5-yl)-2-methoxyphenyl]carbamate Hydrochloride
Phenyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed 4-pyridylmethanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give 2-pyridylmethyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-cr]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (11 mg, 0.023 mmol). The solid was dissolved in ethyl acetate (2 mL) and 1.0Ν ΗC1 in ether (0.1 mL) was added slowly. The precipatate was collected through filtration under nifrogen to give 4-pyridylmethyl N-[4-(4-annno-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_f]pyrimidin-5-yl)-2-methoxyphenyljcarbamate hydrochloride (12 mg, 0.023 mmol). 1Η ΝMR (DMSO-d6) δ 1.91(m, 2Η), 2.16(m, 2H), 3.55 (m, 2H), 3.90 (s, 3H), 4.03 (m, 2H), 4.92 (m, IH), 5.34 (s, 2H), 7.06 (d, J=8.2Hz, IH), 7.16 (s, IH), 7.73 (m, IH), 7. 81 (m, IH), 7.87 (s, IH), 8.46(s, IH), 8.76 (d, J=5.6Hz, IH), 9.05 (s, IH). LC/MS: MH 475.

Example 253
(5-Methyl-3-isoxazolyl)methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyrimidin-5-yl)-2-memoxyphenyl]carbamate
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-c?]pyri.midin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed with (5-methyl-3-isoxazolyl)methanol (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give (5-methyl-3-isoxazolyl)methyl N-[4-(4-an__ino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-J]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (18 mg, 0.038mmol). 1Η ΝMR (CDCl-c δ 2.06(m, 4H), 2.44 (s, 3H), 3.64 (m, 2H), 3.91 (s, 3H), 4.13 (m, 2H), 4.96 (m, IH), 5.26 (s, 2H), 6.12(s, IH), 6.95 (s, IH), 7.06 (m, 2H), 7.39 (s, IH), 8.17 (bs, IH), 8.21(s, IH). LC/MS: MH+ 479.

Example 254
[(2S)-5-Oxotefrahydro-lH-2-pynolyl]methyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_ ]pyriι din-5-yl)-2-methoxyphenyl]carbamate
Phenyl N-[4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-ρynolo[2,3-__]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (30 mg, 0.065 mmol) was mixed with (5S)-5-(hydroxymethyl)tetrahydro-lH-2-pynolone (0.05 mL) in pyridine (0.5 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give [(2S)-5-oxotetrahydro-lH-2-pynolyl]methyl N-[4-(4-amino-7-tetrahydro-2H-4-p5 ranyl-7H-pynolo[2,3--/]pyrimidin-5-yl)-2-methoxyphenyl]carbamate (10 mg, 0.021mmol). 1Η ΝMR (CDCl-d) δ 1.90 (m, 1Η), 2.06(m, 4Η), 2.34 (m, IH), 2.41 (m, 2H), 3.64 (m, 2H), 3.94 (s, 3H), 4.04(m, 2H), 4.14 (m, 2H), 4.98 (m, IH), 5.33 (m, 3H), 6.10(s, IH), 6.98 (s, IH), 7.04 (s, IH), 7.09 (m, IH), 7.31(s, IH), 8.11 (bs, IH), 8.32 (s, IH). LC/MS: MH+ 481.

Example 255
4-Aminobenzyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_f]pyrimidin-5-yl)-2-methoxyphenyl)carbamate

a) tert-Butyl N-(4-(hydroxymethyl)phenyl)carbamate
(4-Aminophenyl)methanol (1.23 g, 10 mmol) and dusopropylethylamine ( 2.6 mL, 15 mmol) was mixed with di-tert-butyl dicarbonate (2.62 g, 12 mmol) in
dichloromethane (50 mL). The mixture was stined at room temperature overnight. Ethyl acetate was added and the organic layer was washed with water, 1.0Ν ΗC1, saturated sodium carbonate, water, brine, dried over MgSO4, filtered and evaporated. The crude product was purified by flash column chromatography with Ethyl acetate/heptane (2:3) to give tert-butyl N-(4- (hydroxymethyl)phenyl)carbamate (2.16g, 9.67 mmol). IH ΝMR (CDCl-d) δ 1.52 (s, 9H), 4.63 (s, 2H), 6.47 (bs, IH), 7.30 (d, 8.5Hz, 2H), 7.36 (d, 8.5Hz,2H).

b) 4-Aminobenzyl N-(4-(4-amino-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- ^pyrirnidin-5-yl)-2-methoxyphenyl)carbamate
Phenyl N-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-_ lpyrimidin-5-yl)- 2-methoxyphenyl]carbamate (51mg, 0.111 mmol) was mixed with tert-butyl N-(4- (hydroxymethyl)phenyl)carbamate (119 mg, 0.533) in pyridine (0.8 mL). The reaction mixture was heated at 100°C overnight. The solvent was removed and the residue was purified by preparative reverse phase LC/MS to give 4-aminobenzyl N- (4-(4-__mino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyi__midin-5-yl)-2-methoxyphenyl)carbamate
(9 mg, 0.015mmol). 1Η ΝMR (CDCl-d) δ 1.52(s, 1Η), 2.08(m, 4Η), 3.65 (m, 2H), 3.90 (s, 3H), 4.14(m, 2H), 4.97 (m, IH), 5.17 (s, 2H), 5.37(bs, IH), 6.55 (s, IH),

6.95 (s, IH), 7.03 (s, IH), 7.06 (m, IH), 7.31(s, IH), 7.38 (m, 3H), 8.16 (bs, IH),

8.30 (s, IH). LC/MS: MH+ 589.

Example 256
Nl-[4-(4-Amino-7-tefrahyα :o-2H-4-ρyranyl-7H-ρynolo[2,3-_ lpyrimidin-5-yl)-2-methoxyphenyl]benzamide
5 -(4-Amino-3 -methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3 -ύ ]pyrimidin-4-amine (80mg, 0.236 mmol) was dissolved in dichloromethane (2.0 mL). Pyridine (2.0 mL) was added followed by benzoyl chloride (41 uL, 0.353 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filfration to give M-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3- ]pyrimidin-5-yl)-2-methoxyphenyl]benzamide (64 mg, 0.144 mmol). 1Η ΝMR (CDCl3-d) δ 2.12 (m, 4Η), 3.67 (m, 2H), 3.99 (s, 3H), 4.17(m, 2H), 4.99 (m, IH), 7.03(s, IH), 7.04 (s, 1H), 7.14 (d, J=8.2Hz, IH), 7.53 (m, 3H), 7.94(d, J=7.8Hz, IH), 8.33 (s, IH), 8.58 (s, IH), 8.63 (d, J=8.2Hz, IH). LC/MS: MH+=444

Example 257
N2-[4-(4-Amino-7-tefrahydro-2H-4-ρvranyl-7H-ρynolo[2,3-_f]ρyrimidin-5-yl)-2- methoxyphenyl]-2-pyridinecarboxamide
5 -(4- Amino-3 -methoxyphenyl)-7-tetrahy dro-2H-4-pyranyl-7H-pynolo [2,3 - < ]pyrimidin-4-amine (80mg, 0.236 mmol) was dissolved in dichloromethane (2.0 mL). Pyridine (2.0 mL) was added followed by 2-pyridinecarbonyl chloride hydrochloride (63 mg, 0.353 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give Nl-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-c lpyrimidin-5-yl)- 2-methoxyphenyl]benzamide (84 mg, 0.189 mmol). 1Η ΝMR (CDCl d) δ 2.12 (m, 4Η), 3.67 (m, 2H), 4.03 (s, 3H), 4.14(m, 2H), 5.00 (m, IH), 5.37 (s, IH), 7.04(s, IH), 7.09 (s, IH), 7.14 (d, J=8.2Hz, IH), 7.50 (m, IH), 7.92 (m, IH), 8.33 (s, IH), ' 8.70(d, J=8.2Hz, IH), 10.62 (s, IH). LC/MS: MH+=445.

Example 258
N5-[4-(4-Annno-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-cTlpyrimidin-5-yl)-2- methoxyphenyl]-l,3-dimethyl-lH-5-pyrazolecarboxamide
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3- _i]pyrimidin-4-amine (80mg, 0.236 mmol) was dissolved in dichloromethane (2.0 mL). Pyridine (2.0 mL) was added followed by 2-pyridinecarbonyl chloride hydrochloride (63 mg, 0.353 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filfration to give N5-[4-(4-__mmo-7-tefrahycfro-2H-4-pyranyl-7H-pynolo[2,3-^pyrinn'din-5-yl)- 2-methoxyphenyl]-l,3-dimethyl-lH-5-pyrazolecarboxamide (30 mg, 0.065 mmol).

1H NMR (CDCl3-_f) δ 2.11 (m, 4H), 2.32 (s, 3H), 3.66 (m, 2H), 3.99 (s, 3H), 4.13(m, 2H), 4.17 (s, 3H), 4.99 (m, IH), 5.22 (bs, 2H), 6.46 (s, IH), 7.03 (s, IH), 7.07 (s, IH), 7.12 (d, J=8.2Hz, IH), 8.33 (2, 2H), 8.49(d, J=8.2Hz, IH). LC/MS: MH+=462.

Example 259
Nl-[4-(4-Amino-7-tefr_ιhyo^o-2H-4-pyranyl-7H-pynolo[2,3-_flpyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylpropanamide
5-(4-Amino-3-methoxyphenyl)-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-_i]pyrimidin-4-amine (50mg, 0.147 mmol) was dissolved in dichloromethane (1.5 mL). Pyridine (1.5 mL) was added followed by 2,2-dimethylpropanoyl chloride (31 mg, 0.221 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filfration to give Nl-[4-(4-ammo-7-tefrahyd o-2H-4-pyranyl-7H-pynolo[2,3-^]pyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylρropanamide (27 mg, 0.064 mmol). 1Η ΝMR (CDC13-d) δ 1.35 (s, 9Η), 2.09 (m, 4H), 3.66 (m, 2H), 3.96 (s, 3H), 4.13(m, 2H), 4.97 (m, IH), 5.46(bs, 2H), 6.98 (s, IH), 7.04 (s, IH), 7.07 (d, J=8.2Hz, IH), 8.15 (s, IH), 8.29 (s, IH), 8.49(d, J=8.2Hz, IH). LC/MS: MH+=424.

Example 260
Nl-[4-(4-Amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-f]pyrimidin-5-yl)-2-methoxyphenyl]- 1 -cyclopentanecarboxamide
5 -(4-Amino-3 -methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo [2,3 -cT]pyrimidin-4-amine (50mg, 0.147 mmol) was dissolved in dichloromethane (1.5 mL). Pyridine (1.5 mL) was added followed by 1-cyclopentanecarbonyl chloride (31 mg, 0.221 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filtration to give Nl- [4-(4-ammo-7-tefr_ihydro-2H-4-pyranyl-7H-pynolo[2,3-_ ]pyriπύdin-5-yl)-2-methoxyphenyl]-2,2-dimethylpropanamide (33 mg, 0.076mmol). 1Η NMR (CDC13-d) 0 I.66 (m, 2Η), 1.81 (m, 2H), 1.95 (m, 4H), 2.06 (m, 4H), 2.77 (m, IH), 3.65 (m, 2H), 3.94 (s, 3H), 4.15(m, 2H), 4.96 (m, IH), 5.37(bs, 2H), 6.98 (s, IH), 7.03 (s, IH), 7.07 (d, J=8.2Hz, IH), 7.84 (s, IH), 8.30 (s, IH), 8.49(d, J=8.2Hz, IH).
LC/MS: MH 437.

Example 261
Nl-[4-(4-Amino-7-tetrahydro-2H-4-pyr__nyl-7H-pynolo[2,3-_ Ipyrimidin-5-yl)-2-methoxyphenyl]-3-phenylpropanamide
5-(4-Amino-3-methoxyphenyl)-7-tetrahydro-2H-4-pyranyl-7H-pynolo[2,3-_ ]pyrimidin-4-amine (50mg, 0.147 mmol) was dissolved in dichloromethane (1.5 mL). Pyridine (1.5 mL) was added followed by 3-phenylpropanoyl chloride (37 mg, 0.221 mmol). After stirring at room temperature for 2 hours, the solvent was removed and the residue was dissolved in 1 ml DMSO, methanol (1 mL) was added and precipitate was formed. The solid was collected by filfration to give Nl-[4-(4-amino-7-tefrahydro-2H-4-pyranyl-7H-pynolo[2,3-cTlpyrimidin-5-yl)-2-methoxyphenyl]-2,2-dimethylpropanamide (7 mg, 0.015mmol). 1Η ΝMR (CDC13-J) δ 2.07 (m, 4Η), 2.75 (m, 2H), 3.09 (m,2H), 3.65 (m, 2H), 3.88 (s, 3H), 4.13(m, 2H), 4.96 (m, IH), 5.97(bs, 2H), 6.93 (s, IH), 7.05 (m, 2H), 7.26 (m, 5H), 7.70 (s, IH), 8.24 (s, IH), 8.46(d, J=8.2Hz, IH). LC/MS: MH+=472.

Examples 262-267 were synthesized using the following procedure:
a)
A mixture of cis-5-(4-amino-3-methoxyphenyl0-7-[4-(4-me1frylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriιmdin-4-amine (0.25 g, 0.575 mmol), pyridine (2.5 ml) and dichloromethane (2.5 ml) was treated with the appropriate acid chloride (0.862 mmol) and then stined at ambient temperature under an atmosphere of nitrogen for 1 hour. The solvents were removed under reduced pressure and the residue was purified by preparative reverse phase chromatography. The compound (280 mg, 0.460 mmol) was dissolved in hot ethyl acetate (25 ml) then treated with maleic acid (160 mg, 1.38 mmol) dissolved in ethyl acetate (10 ml) the mixture was allowed to cool to ambient temperature then stined for 1 hour. The solid was collected by filtration and dried to give the compound as the trimaleate salt. (370 mg).

Analytical RP-HPLC RT listed in the table were obtained on a Hypersil HS C18 column ((5 um, 100A) 250 x 4.6 mm) using a linear gradient of 25-100%
acetonitrile/0.1 M ammonium acetate over 10 min at lml/min. Retention time is indicated by "RT" Mass spectrum molecular weights are indicated by "MH+".



RT 6.62
MH+ 576.3
Gradient a


xamp e RT 7.7 MH+ 608.2 Gradient a



RT 14.23 MH+ 588.3 Gradient b


xamp e RT 6.85 MH+540.2 Gradient a


Example 266 RT 8.15 MH+ 608.2 Gradient a

Example 267 RT 8.15 MH+ 642.3

General salt formation procedure:
Trans- benzyl N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-__]pyrimidin-5-yl}-2-methoxyphenyl)carbamate was dissolved in ethylacetate and treated with maleic acid (280 mg) in ethylacetate. The resulting solid was filtered under a stream of nitrogen and dried in vacuo for 4 hr to give Cw-beι__zyl N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- <i]pyrimidin-5-yl}-2-methoxyphenyl)carbamate tri-maleate salt (580 mg) as a cream solid. M.pt. 158°C (dec.) Η ΝMR (d6 DMSO, 400 MΗz):8.74 (IH, s), 8.27 (IH, s),

7.78 (IH, d), 7.35-7.77 (5H, m), 7.10 (IH, s), 7.04 (IH, s), 6.16 (6H, s), 5.17 (2H, s), 4.74 (IH, m), 3.82 (3H, s), 3.23 (5H, m), 2.78 (3H, s), 2.51 (3H, m), 2.41 (IH, s),

2.09 (4H, m), 1.70 (4H, m). HPLC: ( 5 to 95% CH3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t- = 13.30 min , 94%>.

In a similar manner were prepared the following salts. The LCMS conditions are described below.

LSMS data: Perkin Elmer
Pecosphere C18, 3m , 33
x 4.6, 3.5 ml/min 100 -100% 50 mM ammonium
acetate to acetonitrile in 4.5
minutes
Ret.
Structure MH+
Time


Example 268: Cis and trΩ/j_.-N -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo [2,3 -d]pyrimidin-5 -yl-2-methoxyphenyl)-3 -phenylpropanamide
To 4- [4-amino-5-(4-amino-3 -methoxyphenyl)-7H-pynolo [2,3 -Jjpyrimidin-7-yl]-l-cyclohexanone (0.8 g, 2.3 mmol) in pyridine/dichloromethane (1:2.5, 45 ml) was added hydrocinnamylchloride (0.57 g, 3.4 mmol) in dichloromethane (2 ml) at 0°C under a flow of nifrogen. The solution was stined at 0°C for 2 hr. The solution was quenched with saturated aquoeus citric acid solution ( 50 ml) and the organic layer was washed with saturated aquoeus citric acid solution ( 2 x 50 ml). Dry, filter and concentrate to leave a brown foam (1.0 g). This was dissolved in dichloroethane (100 ml) and N-methylpiperazine (0.63 g, 6.3 mmol) and acetic acid (0.38 g, 6.3 mmol) was added. Sodium triacetoxyborohydride (0.67 g, 3.15 mmol) was added portionwise under nitrogen and the mixture stined overnight at room temperature. Quench with saturated aq. NaHCO3 solution (50 ml) and extract with
dichloromethane (3 x 100 ml). The combined organics were dried (sodium sulphate), filtered and evaporated to leave a sludge which was purified by flash silica gel column chromatography using dichloromethane / methanol (100/0 to 50/50 in 5%increments). The fractions conesponding to the faster running material were combined to give ct_.- Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_/]pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide (0.26 g) as a glass. This was dissolved in ethylacetate (5 ml) and maleic acid (160 mg) in ethylacetate (2 ml) added. The resulting solid was filtered to give cis- Nl-(4-4-annno-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide trimaleate salt (260 mg) as a white solid. Analytical LC/MS conditions: Column: Pecosphere, C18, 3 um, 33x4.6 mm. Eluent: 0% B/A to 100%) B/A in 4.5 min.( B: acetonitrile, A: 50 mM ammonia acetate buffer, PΗ 4.5) , 3.5 mL/min. (rt = 2.86 mins, 568.4).
The fractions conesponding to the slower running material were combined to give trans-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidm-5-yl-2-methoxyphenyl)-3-phenylpropanamide (0.11 g ) as a glass. This was dissolved in ethylacetate (5 ml) and treated with a solution of maleic acid (68 mg) in ethylacetate (2 ml). The resulting solid was filtered to give trans-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-(i]pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide tri-maleate (94 mg) as a white solid.
Analytical LC/MS conditions: Column: Pecosphere, C18, 3 um, 33x4.6 mm. Eluent: 0%. B/A to 100%) B/A in 4.5 min.( B: acetonitrile, A: 50 mM ammonia acetate buffer, PΗ 4.5) , 3.5 mL/min. (rt = 2.68mins, 568.2).

4-[4-amino-5-(4-amino-3-methoxyphenyl)-7H-pynolo[2,3-- ]pyrimidin-7-yl]-l-cyclohexanone (2.25 g, 6.5 mmol), acetic acid (1.17 g, 19.5 mmol) and N-methylpiperazine (1.95 g, 19.5 mmol) were dissolved in dichloroethane (200 ml). Sodium triacetoxyborohydride (2.07 g, 9.75 mmol) was added portionwise and the mixture stined at room temperature overnight. Saturated sodium bicarbonate solution (150 ml) was added and the aqueous layer exfracted with dichloromethane (3 x 100 ml). The combined organics were washed with water, dried (sodium sulphate), filtered and evaporated to leave a semi-solid whaich was purified by flash silica gel column chromatography using CΗ2C12 / methanol (0% MeOH to 50% MeOH in 5%> increments). The fractions conesponding to the faster running material were combined and evaporated to give cis- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_t]pyrimidin-4-amine (1.2 g, 43%) as a cream solid. Η NMR (d6-DMSO): δ 8.1 (1Η, s), 7.11 (1Η, s), 6.87 (1Η, s), 6.79 (1Η, d), 6.05 (2Η, bs), 4.80 (2H, bs), 4.64 (IH, m), 4.08 (IH, m), 3.82 (3H, s), 3.17 (2H, m), 2.37 (6H, m), 2.21 (3H, s), 2.08 (4H, m), 1.70 (2H, m), 1.53 (2H, m).
HPLC (rt= 11.24 min, 97.6%).
The fractions conesponding to the slower πrnning material were combined and evaporated to give trans- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-4-amine (0.4 g, 14%>) as a white solid. Η NMR (d6-DMSO): δ 8.10 (1Η, s), 7.26 (1Η, s), 6.87 (1Η, s), 6.77 (1H, d), 6.71 (IH, d), 6.05 (2H, bs), 4.79 (2H, s), 4.52 (IH, m), 3.81 (3H, s), 3.35 (IH, m), 2.50 (5H, m), 2.31 (5H, m), 2.14 (IH, m), 1.97 (6H, m), 1.45 (2H, m). HPLC (rt= 10.13 min, 97.9%).

To a solution of cis- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-memylpiperazino)cyclohexyl]-7H-pynolo[2,3-^pyrimidin-4-amine (30 mg, 0.069 mmol) in pyridine (0.5 ml) was added the appropriate acid chloride (2 eq., 0.138 mmol). . The vials were capped and shaken overnight on an orbital shaker. Another two equivalent of acid chlorides (0.138 mmol) was added in two portions (1 equivalent each) and the resulting mixtures were shaken overnight again. LCMS (Micromass- Column: Pecosphere, C18, 3 um, 33x4.6 mm. Eluents: 0% B/A to 100%) B/A in 4.5 min.( B: acetonitrile, A: 50 mM ammonia acetate buffer, PΗ 4.5) , 3.5 mL/min.) ofthe resulting mixtures showed presence of product in all cases. The solutions were evaporated to dryness and the resulting residues were re- dissolved in a small volume of DMF and purified by reverse phase prep. ΗPLC. The structures are detailed below alongwith the appropriate LCMS data.

Examples 269 to 293 were made by methods analogous to Example 268.


Example 269
RT 2.61
MΗ+ 576.3


Example 270
RT 3.02
MH+ 570.3


10 Example 271
RT 2.61
MH+ 600.3

15


Example 272
RT 3.26

20 MH+ 608.3


Example 273
RT 2.74
MH+ 570.3



10 Example 274
RT 2.78
MH+ 558.4

15


Example 275
RT 3.00

20 MH+ 574.3


Example 276

10 RT 2.76
570.3



15
Example 277
RT 3.26
MH+ 608.3

20

Example 278
RT 2.94

10 MH+ 570.3



15
Example 279
RT 3.13
MH+ 604.3

20

Example 280
RT 3.16
580.3

10

15



20
Example 281
RT 2.68
MH+ 565.3

25


10 Example 282
RT 2.90
MH+ 585.3

15



20 Example 283
RT 2.84
MH+ 585.3

25

Example 284
RT 2.90
MH+ 576.3

10


15 Example 285
RT 2.90
MH+ 584.4

20

25

Example 286
RT 2.74
MH+ 565.6

10

15



Example 287
RT 3.06

20 MH+ 576.3

25


Example 288
RT 2.53
MH+ 575.3

10

15


Example 289
RT 3.32

20 MH+ 624.3


Example 290
RT 2.85
MH+ 594.4


10 Example 291
RT 2.76
MH+ 592.3



15
Example 292 RT 2.86
MH+ 583.3



Example 293
RT 2.29
MH+ 508.3

General Synthesis for examples 294-301:

Method A
A mixture ofthe appropriate piperazine (7.60 mmol), 4-[4-amino-5-(4-phenoxyphenyl)- 7H-pynolo[2,3-_i]pyrimidin-7-yl]-l-cyclohexanone (2.53 mmol), and glacial acetic acid

(7.60 mmol) in 50 mL of dichloroethane was stined at room temperature for 1.5 hours.

Sodium triacetoxyborohydride (3.28 mmol) was added and the mixture was stined at room temperature for 16 hours. A solution of 1.35 g of sodium bicarbonate in 50 mL of water was added and the reaction mixture was stined for 1 hour. The organic portion was separated, dried over magnesium sulfate, filtered, and the filtrate concenfrated to afford a brown oil. Purification by flash chromatography on silica gel afforded the cis-and trα«5-7-[(4-piperazino)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-4-amines.

Method B
A mixture of the appropriate pynolidine (7.53 mmol), 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrimidin-7-yl]-l-cyclohexanone (2.51 mmol), and glacial acetic acid (7.35 mmol) in 45 mL of dichloroethane was stined at room temperature for 30 minutes. Sodium triacetoxyborohydride (3.26 mmol) was added and the mixture was stined at room temperature for 22 hours. A solution of 1.35 g of sodium bicarbonate in 50 mL of water was added and the reaction mixture was stined for 1 hour. The organic portion was separated, dried over magnesium sulfate, filtered, and the filtrate concentrated to afford a brown oil. Purification by flash chromatography on silica gel afforded the cis- and tr_z«>s'-7-(4-pynolidino)cyclohexyl]- 5-(4-phenoxyphenyl)-7H-pynolo[2,3-_f|pyrimidin-4-arnines.

Salt Formation
To a warm solution of pyπolopyrimidine (2.48 mmol; from methods A or B, above) in ethanol was added a solution of maleic acid (7.28 mmol) in ethanol. A white precipitate formed as the solution was cooled to ambient temperature. The resulting solid was isolated by filtration and dried under vacuum to yield the desired tris maleate salt.

Analytical RP-ΗPLC RT listed in the table were obtained on a Hypersil HyPurity Elite

C18 column ((5uM, 200 A) 250 x 4.6 mm) using a linear gradient of 25-100% acetonitrile/0.1 M ammonium acetate over 10 min. (gradient a) or 25 min. (gradient b) at 1 mL/min.


RT 7.967
MH+ 511.1
Gradient a



RT 7.383
MH+ 527.2
Gradient a


10 Example 296
RT 13.941
MH+ 497.1
Gradient b



RT 7.733
MH+ 511.2
Gradient a


Example 298
RT 14.067

10 MH+ 497.1
Gradient b

Example 299
RT 13.891
MH+ 497.1
Gradient b



Example 300
RT 14.076
MH+ 497.1
Gradient b
15

Example 301
RT 7.750
MH+ 527.2
Gradient a

Example 302: Cis and trans 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-7-yl]- 1-hydroxycyclohexylmethyl cyanide

A solution of diisopropylamine (0.649 g, 0.0050 mol) in tefrahydrofuran (10 mL) was cooled to 0° C. A solution of 1.6 M n-butyl lithium ( 3.14 mL, 0.0050 mol) in hexanes was added dropwise, keeping the temperature less than 5° C. After the addition was complete, the mixture was stined for 20 minutes at 0° C. The mixture was cooled to -78° C, and dry acetonitrile (0.175 g, 0.0043 mol) was added, keeping the temperature less than -70° C. After the addition was complete, the mixture was stined for 20 minutes at -78° C, and a mixture of 4-[4-amino-5-(4-phenoxyρhenyl)-7H-pynolo[2,3-ti]pyrimidin-7-yl]-l-cyclohexanone (1.000 g, 0.0025 mmol) in tetrahydrofuran (10 mL) and hexamethylphosphoramide (10 mL) was added, keeping the temperature less than -70° C. After the addition was complete, the mixture was stined for 30 minutes at -78° C, then stined at ambient temperature for 18 hours. The mixture was partitioned between dichloromethane and saturated ammomum chloride (aq). The organic phase was washed with water and saturated sodium bicarbonate (aq), and dried over magnesium sulfate. The solvent was removed in vacuo and the cis and trans isomers were separated by flash column chromatography on silica using dichloromethane/methanol (95:5) as an eluent to give less polar 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrinιidin-7-yl]-l-hydroxycyclohexylmethyl cyanide (0.120g, 0.00027 mol) and more polar 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-7-yl]-l-hydroxycyclohexylmethyl cyanide (0.170g, 0.00038 mol):

Less polar:
Η NMR (DMSO- d 400MΗz) δ 8.13 (s, IH), 7.48 (d, 2H), 7.41 (t, 2H), 7.37 (s, IH), 7.15 (t, IH), 7.093 (d, 2H), 7.088 (d, 2H), 6.11 (b, 2H) 5.05 (s, IH), 4.53-4.61 (m, IH), 2.66 (s, 2H), 2.18 (q, 2H), 1.80 (t, 4H) 1.66 (t, 2H); RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%>-85%> acetonitrile - 0.1M ammomum acetate over 20 min, lmL/min) R_ 15.90. MH+ 440.

More polar: (Probably trans, aryl-axial, OH-axial)
Η NMR (DMSO- 6j 400MHz) δ 8.13 (s, IH), 7.63 (s, IH), 7.48 (d, 2H), 7.41 (t, 2H), 7.15 (t, IH), 7.11 (d, 2H), 7.08 (d, 2H), 6.11 (b, 2H) 5.22 (s, IH), 4.62-4.67 (m, IH), 2.98 (s, 2H), 1.82-1.99 (m, 6H), 1.65-1.73 (m, 2H); RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%>-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) R, 15.88. MET 440.

Example 303: cis- and trans- 5-(4-amino-3-fluorophenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-4-amine

a) tert-Butyl N-(4-bromo-2-fluorophenyl)carbamate
Sodium bis(trimethylsilyl)amide solution (l.O soln. in THF, 2.05 equiv., 270 mL, 270 mmol) was added dropwise to a solution of 4-bromo-2-fluoroaniline (24.78 g, 130.4 mmol) in THF (250 mL) over 15 min. under nitrogen. After a further 15 min., di-tert-buxyl dicarbonaxe (1.2 equiv., 34.12 g, 156.3 mmol) was added portionwise (note: a slight exotherm was observed). The reaction became very viscous and after 4 h. reached completion (t.l.c. analysis using 1:9 EtOAc:heptane as the eluent). The reaction was concenfrated in vacuo and the residue was partitioned between EtOAc (300 mL) and saturated aq. NaHCO3 (150 mL). The aqueous layer was further exfracted EtOAc (2 x 200 mL) and the combined organic layers were dried (NajSO and concentrated under reduced pressure. Purification by column chromatography using a 10%> to 15%> EtOAc : heptane gradient afforded tert-butyl N-(4-bromo-2-fluorophenyl) carbamate a light yellow waxy solid (30.0 g, 79%), Η NMR (400 MHz, CDC13) 1.51 (9H, s), 7.22 (IH, m), and 7.24 (2H, m).

b) tert-Butyl N-[2-fluoro-4-(4,4,5,5-teframethyl-l,3,2-dioxaborolan-2-yl)phenyl] carbamate
A solution ofthe tert-butyl N-(4-bromo-2-fluoroρhenyl)carbamate (54.0 g, 0.186 mmol), to-pinacolatodiborane (1.2 equiv, 56.8 g, 223.3 mmol), potassium acetate (3.0 equiv, 54.7 g, 558 mmol) and PdCl2(dppf) (0.03 equiv, 4.65 g, 5.58 mmol) in degassed DMF (1 1) was heated at 80°C under nitrogen for 16 h. The DMF was removed under reduced pressure and the resulting dark solid residue was dissolved in CH2C12 (500 mL). The inorganic residues were removed by filtration through a silica gel pad and the filtrate was purified by column chromatography using a 10% to 15% EtOAc : heptane gradient to afford the product as a yellow viscous oil which crystallized on standing to give tert-butyl N-[2-fluoro-4-(4,4, 5, 5-tetramethyl-1, 3, 2-dioxaborolan-2-yl)phenyl] carbamate (56.5 g, 92 %), Η NMR (400 MHz, CDCI3) 1.33 (12 H, s), 1.53 (9 H, s), 6.82 (IH, br s), 7.46 (IH, d, J 11 Hz), 7.55 (1 H, br d), and 8.12 (1 H, br t), m/z 337.2, and RP-HPLC (5 to 100 % CH3CN in 0.1 N aqueous ammonium acetate over 15 min at 1 mL/min using a Hypersil HyPurixy Elite C18, 5m, 200 A, 250 x 4.6 mm column) t== 10.16 min, 90%.

c) tert-Butyl N-4-[4-chloro-7-(l ,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenylcarbamate
A suspension ofthe 4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-5-iodo-7H- pynolo[2,3-d]pyrimidine (31.18 g, 74.41 mmol), tert-butyl N-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl]carbamate (1.5 equiv, 37.6 g, 111.6 mmol), sodium carbonate (2.5 equiv, 19.72 g, 186 mmol) and Pd(PPh3)4 (4 mol%o, 3.44 g, 2.98 mmol)in DME (1.2 1) and degassed H2O (230 mL) was heated at 80°C under nitrogen for 17 h. Additional Pd catalyst (1 mol%o, .86 g, 0.74 mmol) was added and the reaction was continued heating at 80°C for a further 24 h. at which point the reaction had proceeded to completion (tl.c. analysis using 3:7
EtOAc:heptane as the eluent, Rf=0.7). The solvent was removed under reduced pressure and the residue dissolved in EtOAc (500 mL) and the inorganics were removed by filtration through a celite pad. The filtrate was washed with 10%> aq. Na2CO3 (200 mL) and brine (200 mL), dried (MgSO4) and concentrated in vacuo. Column chromatography purification over silica gel using 1 :2 EtOAc:heptane afforded tert-butyl N-4-[4-chloro-7-(l ,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenylcarbamate as an off-white solid (21.0 g, 56%), 2H NMR (400 MHz, CDC13) 1.55 (9 H, s), 1.89 (4 H, m), 2.07 (4 H, m), 4.01 (4 H, s),

4.89 (1 H, m), 6.75 (1 H, br s), 7.23 (1 H, br s), 7.25 (1 H, br s), 7.34 (1 H, br s),

8.14 (1 H, br t), and 8.64 (1 H, s) and RP-HPLC (5 to 100 % CH3CN in 0.1 N aqueous ammonium acetate over 15 min at 1 mL/min using a Hypersil HyPurity

Elite C18, 5μm, 20θA, 250 x 4.6 mm column) t_= 10.48 min., 100%.

d) 5-(4-Amino-3-fluorophenyl)-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-d]pyrimidin-4-amine
A cloudy mixture of tert-butyl N-4-[4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7Η-pyπolo[2,3-d]pyrimidin-5-yl]-2-fluorophenylcarbamate ( 10.5 g, 20.92 mmol), aq. ammonium hydroxide (28 - 30 %, 100 mL) and dioxane (100 mL) was placed in a sealed vessel at ambient temperature then heated to 120°C with stirring for 24 h. (t.l.c. analysis using 9:1 EtOAc:heptane as the eluent). The reaction was concentrated in vacuo, diluted with EtOAc (300 mL), washed with brine (2x 150 mL), dried (Na^O^ and concenfrated under reduced pressure and scrupulously dried to afford 5-(4-Amino-3-fluorophenyl)-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine as a yellow solid (7.93 g, 99 %), 'H NMR (400 MHz, d6-DMSO) 1.74 (4 H, m), 1.90 (2 H, m), 2.06 (2 H, m), 3.90 (4 H, rn), 4.64 (1 H, m), 5.18 (2 H, br s), 6.02 (2 H, br s), 6.84 (1 H, t), 6.97 (1 H, d), 7.08 (1 H, d), 7.26 (1 H, s) and 8.10 (1 H, s) and m/z 384.2 (MH ).

e) 4-[4-amino-5-(4-amino-3-fluorophenyl)-7H-pynolo[2,3-cT]pyrimidin-7-yl]-l-cyclohexanone
5M ΗC1 (300 mL) was added slowly to a solution of 5-(4-amino-3-fluorophenyl)-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7Η-pynolo[2,3-d]pyrimidin-4-amine (18.49 g, 48.28 mmol) in acetone (800 mL) at 0°C the resulting dark orange-brown solution was heated at 60°C for 4 h. (t.l.c. analysis using 10%> MeOH in CH2C12). The acetone was removed under reduced pressure and the acidic layer was basified to approx. pH 8 using sat. aq.
The resulting precipitate was collect by filtration and scrupulously dried to afford 4-[4-amino-5-(4-amino-3-fluorophenyl)-7H-pyrrolo[2,3-d]pyrimidin-7-yl]-l-cyclohexanone as a light brown solid (12.67 g, 77%>). A second crop was also obtained from the mother liquor on standing (2.01 g, 12%), Η NMR (400 MHz, d6-DMSO) 2.27 (2 H, m), 2.30 (4 H, br d), 2.73 (2 H, m), 5.14 (1 H, m), 5.20 (2 H, br s), 6.05 (2 H, br s), 6.85 (1 H, t), 6.97 (1 H, dd), 7.06 (1 H, dd), 7.35 (1 H, s) and 8.12 (1 H, s) and m/z 340.1 (MLX).

cis- and trans- 5-(4-Amino-3-fluorophenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-./]pyrimidin-4-amine

Example 304: cis-Nl -(4- {4-an ino-7-[4-(4-methylpiρerazino)cyclohexyl]-7Η-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l-benzenesulfonamide tri-maleate
To a solution of 4-[4-amino-5-(4-amino-3-fluorophenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l-cyclohexanone (1.0 g, 2.95 mmol), N-methylpiperazine (3 equiv, 0.885 g, 8.85 mmol, 0.98 mL) and glacial acetic acid (3 equiv., 0.51 mL, 8.85 mmol) in dichloroethane (50 mL) under nifrogen was added sodium
triacetoxyborohydride (1.3 equiv., 0.81 g, 3.84 mmol). The solution was stined for 18 hr then additional sodium triacetoxyborohydride (0.40 g, 1.9 mmol) was added and the reaction continued for a further 48 hr. The reaction was concentrated in vacuo, partitioned between dichloromethane (100 mL) and sat. aq. NaHCO3 (100 mL). The aqueous layer was further extracted with dichloromethane (4 x 100 mL) and the combined organic layers were dried over magnesium sulfate and evaporated to dryness to give a yellow foam (0.95 g). Purification by column chromatography over silica gel using a dichloromethane:methanol (9:1 to 5:1) gradient afforded cis- 5-(4-amino-3-fluorophenyl)-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pyrrolo[2, 3- dJpyrimidin-4-amine,t e higher running component, as a cream solid (400 mg, 32 %) Η NMR (d6 DMSO, 400 MHz) 1.56 (3H, br t), 1.68 (2H, br d), 1.99 (5H, m), 2.20 (3H, s), 2.43 (7H, br m), 4.65 (IH, m), 5.20 (2H, s), 6.01 (2H, br s), 6.85 (IH, t, J= 9.6 Hz), 6.98 (IH, dd, J= 8.0 and 1.6 Hz), 7.10 (IH, dd, J=12.4 and 1.6 Hz), 7.12 (IH, s), and 8.10 (IH, s) and RP-HPLC (10 to 90 % CH3CN in 0.1 N aqueous ammonium acetate over 12 min at 2 mL/min using a Waters Symmetry C18, 250 x 4.6 mm column) tr= 8.619 min., 96%
A mixed fraction was obtained which contained both cis- and trαns-isomers (440 mg, 50:50 mixture), and in addition the lower running fraction contained trans-5-(4- amino-3-fluorophenyl)-7-[4-(4-methylpiperazino)cyclohexylJ- 7H-pyrrolo[2, 3-' d]pyrimidin-4-amine, as a yellow solid (110 mg, 9%), 'H NMR (d6 DMSO, 400 MHz) 1.94 (6H, m), 2.17 (3H, s), 2.33 (7H, br m), 2.51 (3H, m), 3.28 (IH, m), 4.51 (IH, m), 5.18 (2H, s), 6.01 (2H, br s), 6.84 (IH, t), 6.96 (IH, dd), 7.04 (IH, dd), 7.30 (IH, s), and 8.08 (IH, s) and RP-HPLC (10 to 40 % CH3CN in 0.1 N aqueous ammonium acetate over 12 min at 2 mL/min using a Waters Symmetry C18, 250 x 4.6 mm column) t= 7.595 min, 97%

Example 305: trans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l-benzenesulfonamide tri- maleate
4-Fluorobenzenesulfonyl chloride (45.9 mg, 0.236 mmol) was added to a solution of frans-5-(4-amino-3-fluorophenyl)-7-[4-(4- methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (100 mg, 0.236 mmol) in pyridine (2 mL) at 40oC. After 27 hr at 40°C the reaction had reached completion and was concenfrated in vacuo. Purification by column chromatography over silica gel using 10%> to 50%> MeOH in dichloromethane as the gradient afforded as a colurless oil (0.78 mmol). The product was dissolved in ethanol and maleic acid (3 equiv., 27 mg, 0.233 mmol) added. The mixture was heated until
homogeneous and trans-Nl-(4-{4-amino- 7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pyrrolo [2, 3-d] pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l -benzenesulfonamide trimaleate crystallised on cooling as a fawn solid (37 mg, 17 %>), RP-HPLC (10 to 40 % CH3CN in 0.1 N aqueous ammomum acetate over 12 min at 2 mL/min using a Waters Symmetiy Cl 8, 250 x 4.6 mm column) = 14.528 min., 96% and m/z 582.0 (MIT).

Example 306: cis-Nl -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l-benzenesulfonamide
cis-Nl-(4{-4-αmino-7-[4-(4-methylpiperαzino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l-benzenesulfonαmide was prepared using the same procedure as detailed for the free base oflxans-Nl-(4{-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l -benzenesulfonamide except on a 3.36 mmol scale.
(400 mg, 32%), RP-HPLC (10 to 40 % CH3CN in 0.1 N aqueous ammomum acetate over 12 min at 2 mL/min using a Waters Symmetry C18, 250 x 4.6 mm column) X = 15.232, 94% min. and m/z = 582.1 (MIT).

Example 307: 5-(4-amino-3-fluorophenyl)-7-(l-benzyl-4-piperidyl)-7H-pyrrolo[2,3-dJpyrimidin-4-amine

a) 7-(l -beι__?yl-4-piperidyl)-4-cMoro-5-iodo-7H-pynolo[2,3-_ ]pyrimidine
Diethyl diazodicarboxylate (2.0 equiv., 18.19 g, 41.2 mL, 104.8 mmol) was added dropwise over approx. 1 h to a solution of 4-chloro-3-iodopyπolo[2,3-d]pyrimidine (14.55 g, 52.4 mmol), l-benzyl-4-hydroxypiperidine (3.0 equiv., 30.06 g, 157.16 mmol) and triphenylphosphine (2.0 equiv., 27.51 g, 104.8 mmol) in THF (730 mL) at room temperature under nitrogen. The reaction reached completion after 72 h (t.l.c. analysis using 1:1 EtOAc:heptane as the eluent, Rf = 0.2). The reaction was concentrated in vacuo and 1 :4 ethyl acetate : heptane was added until a precipitate in a clear solution was observed. The precipitate was collected by filtration (Ph3PO) and the filtrate was concentrated, dissolved in ethyl acetate (500 mL) and extracted with aq. HCI (IM, 3 x 200 mL). The combined acidic layers were basified with aq NaOH (4 N) to pH 12 then extracted into ethyl acetate(3 x 300 mL), dried (MgSO4) and concentrated in vacuo. Purification by column
chromatography using 5:4 light petroleum (30-60 °C):ethyl acetate over silica gel gave 2 main fractions of which the first fraction contained the product as a pale yellow crystalline solid which was recrystallised from ethyl acetate to give 7-(l- benzyl-4-piperidyl)-4-chloro-5-iodo-7H-pynolo[2,3-_/]pyrimidine as a cream crystalline solid (5.7 g, 24 %); Η NMR (400 MHz, CDC13) 2.02 (4H, m), 2.24 (2H, m), 3.06 (2H, br d), 3.58 (2H, s), 4.76 (IH, m), 7.27 (2H, m), 7.32 (3H, m), 7.49 (IH, s) and 8.60 (IH, s) and m/z = 452.8 (ME ).

b) tert-butyl N-4-[7-(l-benzyl-4-piperidyl)-4-chloro-7H-pynolo[2,3-_ lpyrimidin-5- yl]-2-fluorophenylcarbamate
A suspension of 7-(l-benzyl-4-piperidyl)-4-chloro-5-iodo-7H-pynolo[2,3- ]pyrimidine (5.7 g, 12.6 mmol), tert-butyl N-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)phenyl]carbamate (1.5 equiv, 18.9 g, 6.38 mmol), sodium carbonate (2.5 equiv, 3.34 g, 31.5 mmol) and Pd(PPh3)4 (4 mol%, 0.58 g, 0.5 mmol) in DME (210 mL) and degassed water (37 mL) was heated at 80°C under nifrogen for 17 h (t.l.c. analysis using 1:1 EtOAc:heptane as the eluent). The reaction mixture was concentrated in vacuo, dissolved in ethyl acetate (400 mL) and washed with 10 % aq. Νa2CO3 (3 x 200 mL). The organic layer was dried (MgSO4), concentrated and purified by column chromatography using 1:1 ethyl acetate:heptane as the eluent to afford tert-butyl N-4-[7-(l-benzyl-4-piperidyl)-4-chloro-7H-pynolo[2,3- _i]pyrimidin-5-yl]-2-fluorophenylcarbamate as a white crystalline solid (5.2 g, 9.7 mmol, 77%), Η ΝMR (400 MHz, CDC13) 1.55 (9 H, s), 2.05 (4H, m), 2.24 (2H, m), 3.06 (2H, br d), 3.60 (2H, s), 4.83 (IH, m), 7.25 (2H, m), 7.29 (IH, m), 7.33 (6H, m), 8.12 (IH, br f) and 8.64 (IH, s).

c) 5-(4-amino-3-fluorophenyl)-7-(l-benzyl-4-piperidyl)-7H-pyrrolo[2, 3-dJpyrimidin-4-amine
A mixture ofthe tert-butyl N-4-[7-(l-benzyl-4-piperidyl)-4-chloro-7H-pynolo[2,3-_ ]pyrimidin-5-yl]-2-fluorophenylcarbamate (5.2 g, 9.7 mmol), aq.
ammonium hydroxide (28 - 30 %, 100 mL) and 1,4-dioxane (100 mL) was placed in a sealed vessel at ambient temperature then heated to 120 °C with stirring for 16 h. (t.l.c. analysis using EtOAc as the eluent). The reaction was concentrated in vacuo, diluted with EtOAc (300 mL), washed with brine (2x 200 mL), dried (Νa^SO^ and concentrated under reduced pressure to afford a brown solid which was triurated with ether (approx. 50 mL) to give 5-(4-amino-3-fluorophenyl)-7-(l-benzyl-4-piperidyl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine as a cream solid (3.0 g, 74 %>), λH ΝMR (400 MHz, CDC13) 2.06 (4 H, m), 2.27 (2 H, m), 3.06 (2 H, m), 3.59 (2H, br s), 3.70 (2 H, br s), 4.73 (IH, m), 5.12 (2 H, s), 6.85 (1 H, t), 7.01(1 H, s), 7.06 (1 H, dd), 7.10 (1 H, dd), 7.28 (2 H, m), 7.34 (3H, m) and 8.31 (1 H, s) and m.p. 141-142 °C.

Example 308: Νl-4-[4-amino-7-(l-benzyl-4-ρiperidyl)-7H-pyπolo[2,3-d]ρyrimidin-5-yl]-2-fluorophenyl-4-fiuoro- 1 -benzenesulfonamide
Nl-4-[4-amino-7-(l-benzyl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l -benzenesulfonamide (470981) was prepared using the same procedure as detailed for frans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-1 -benzenesulfonamide tri-maleate except on a 6.96 mmol scale. Nl-4-[4-amino-7-( 1 -benzyl-4-ρiperidyl)-7H-pyπolo [2,3 -d]pyrimidin-5 -yl] -2-fluoroρhenyl-4-fluoro-1 -benzenesulfonamide was obtained as a cream solid (3.2 g, 80%), m/z 575 (MET) and m.p. 265-6 °C.

Example 309: Nl-4-[4-amino-7-(l-benzyl-4-piperidyl)-7H-pynolo[2,3- d]pyrimimn-5-yl]-2-fluorophenyl-2,3-dicMoro-l-benzenesulfonamide
Nl-4-[4-amino-7-(l-ben__yl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]- 2-fluorophenyl-2,3-dichloro-l -benzenesulfonamide was prepared in the same manner as detailed above on a 5.04 mmol scale. The resulting Nl-4-[4-amino-7-(l-benzyl-4-piperidyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l-benzenesulfonamide was obtained as a brown solid (1.0 g, 32%>), m/z 625 (MET") and

RP-HPLC (5 to 85 % CH3CN in 0.1 N aqueous ammomum acetate over 20 min at 1 mL/min using a Waters Delta pack 5m Cl 8, 30θA, 150 x 3.9 mm column) t =

14.963 min., 95%.

Example 310: Nl -4-[4-amino-7-(4-ρiperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro- 1 -benzenesulfonamide
A mixture containing Nl-4-[4-amino-7-(l-benzyl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l-benzenesulfonamide (2.40 g, 4.18 mmol), ammonium formate (10 equiv., 41.8 mmol, 2.62 g), palladium on carbon (10%, 1.2g) and ethanol (100 mL) was heated at reflux with vigorous stirring for 6h., filtered and concenfrated in vacuo. The solid was partitioned between dichloromethane (50 mL) and water (50 mL). The brown solid which formed at the phase boundary was collected and analysed for Nl-4-[ '4-amino-7-(4-piperidyl)-7H-pyrrolo[2, 3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l-benzenesulfonamide (0.33 g), m/z 485 ( H-) and m.p. 238-9 °C (dec).

Example 311: Nl-4-[4-amino-7-(l-formyl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluoroρhenyl-4-fluoro- 1 -benzenesulfonamide
The procedure detailed for the preparation of Nl-4-[4-amino-7-(4-piperidyl)- 7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l-benzenesulfonamide was performed on a smaller scale (0.35 mmol) where the combined organic layers from the work up were isolated, dried (Na-SO4) and the solvent removed under reduced pressure to afford a white oil which was purified by preparative HPLC (100% pH 4.5 50mM ammonium acetate to 100% CH3CN in 8.5 minutes with a 1.5 minute hold at 25 mL/min using a Hypersil 5m BDS C18, lOOx 21.2mm column) to yield Nl -4-[4-amino- 7-(l-formyl-4-piperidyl)-7H-pyrrolo[2, 3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l -benzenesulfonamide as a white solid (50 mg, 27 %), m/z = 512.9 (MIT) and RP-HPLC (5 to 85 % CH3CN in 0.1 N aqueous ammonium acetate over 20 min at 1 mL/min using a Waters Delta pack 5m C18, 300A, 150 x 3.9 mm column) t = 13.091 min., 95%.

Example 312: Nl-[4-(4-amino-7-l-[(l-methyl-lH-4-imidazolyl)sulfonyl]-4-piperidyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide dimaleate
l-Methylimidazol-4-yl sulphonyl chloride (1.1 equiv., 0.068 mmol, 12.3 mg) was added to a suspension of 5-(4-amino-3-fluorophenyl)-7-(l-benzyl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-4-amine (30 mg, 0.062 mmol) and triethylamine (3 equiv., 0.186 mmol, 26 1) in dichloromethane (1 mL) and stined at ambient temperature for 24 h. The reaction was concentrated in vacuo, partitioned between dichloromethane (100 mL) and water (50 mL) and the aqueous layer was further exfracted with dichloromethane (3 x 100 mL). The combined organic layers were dried over magnesium sulfate and concentrated in vacuo. Purification by column chromatography over silica gel using 10 % methanol in dichloromethane yielded a waxy white solid (10 mg). Maleic acid (2 equiv., 4 mg) was added to the product in hot ethanol and Nl-[4-(4-amino-7-l-[(l-methyl-lH-4-imidazolyl)sulfonyl]-4-piperidyl- 7H-pyrrolo[2, 3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l -benzenesulfonamide dimaleatesaXt crystallized on cooling (10 mg), RP-HPLC (5 to 85 % CH3CN in 0.1 N aqueous ammonium acetate over 20 min at 1 mL/min using a Waters Delta pack 5m C18, 30θA, 150 x 3.9 mm column) t_= 14.186, 100% min. and m/z = 629 ( H+).

Example 313: Nl-[4-(4-amino-7-l-[(l,2-dimexhyl-lΗ-4-imidazolyl)sulfonyl]-4-piperidyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide
Using the procedure detailed for the synthesis ofthe free base of Nl-[4-(4- an___no-7-l-[(l-methyl-lH-4-imidazolyl)sulfonyl]-4-piperidyl-7H-pynolo[2,3- d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l -benzenesulfonamide dimaleate, Nl-[4-(4-amino-7-l-[(l,2-dimethyl-lH-4-imidazolyl)sulfonyl]-4-piperidyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-bexιzenesulϊonamide was prepared as a cream solid (9 mg), m.p. 217-8 °C and m/z = 643.2 (MH+).

Example 314: Nl-[4-(4-amino-7-l-[(l,3-dimethyl-lH-5-ρyrazolyl)carbonyl]-4-piperidyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide
1, 3 -Dimethylpyrazole-5 -carbonyl chloride (1.5 equiv., 14.8 mg, 0.093 mmol) was added to a stined suspension of 5-(4-amino-3 -fluorophenyl)-7-( 1 -benzyl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-4-amine (30 mg, 0.062 mmol) and potassium carbonate (2 equiv., 17.1 mg, 0.124 mmol) in N-methylpynolidinone (2 mL) and the resulting mixture was stined at ambient temperature under nifrogen for 16 h. The solvent was removed in vacuo and the mixture purified by column chromatography over silica gel using 5 %> methanol in dichloromethane as the eluent to give Nl-[4-(4-αmino- 7-1 -[(1, 3-dimethyl-lH-5-pyrαzolyl)cαrbonyl]-4-piperidyl- 7H-pyrrolo[2, 3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonαmide as a colourless glass (10 mg), RP-HPLC HPLC (100% pH 4.5 50mM ammonium acetate to 100% CH3CN in 4.5 minutes with a 0.5 minute hold at 3.5 mL/min using a Perkin Elmer Pecosphere 3m Cl 8 (33 x 4.6mm) column) t = 2.98 min., 96% and m/z = 629 (MIT).

Example 315: Nl-(4-{4-amino-7-[l-(2-pyridylcarbonyl)-4-piperidyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-fluoro-l-benzenesulfonamide
Nl-(4-{4-amino-7-[l-(2-pyridylcarbonyl)-4-piperidyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-4-fluoro- 1 -benzenesulfonamide was prepared using the same procedure as detailed for Nl-[4-(4-amino-7-l-[(l,3-dimethyl-lH-5-pyrazolyl)carbonyl]-4-ρiperidyl-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-4-fluoro-l-benzenesulfonamide, (12 mg), RP-HPLC HPLC (100% pH 4.5 50mM ammomum acetate to 100% CH3CN in 4.5 minutes with a 0.5 minute hold at 3.5 mL/min using a Perkin Elmer Pecosphere 3m C18 (33 x 4.6mm) column) t = 2.73 min., 98% and m/z = 590.2 (MET).

Example 316: Nl -4-(4-amino-7- {4-[ 1 -( 1 -methylpiperid-4-yl)piperidyl]-7H-pynolo[2,3-d]pyrimidm-5-yl})-2-fluorophenyl-4-fluoro-l-benzenesulfonamide trimaleate
Sodium triacetoxyborohydride (28.1 mg, 0.134 mmol) was added to a solution of Nl-4-[4-amino-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l -benzenesulfonamide (50 mg, 0.103 mmol) and 1-methylpiperid-4-one (0.92 ml, 0.155 mmol) in glacial acetic acid (0.025 mL) and NMP (3 mL). The reaction was stined for 20 h at room temperature then additional sodium triacetoxyborohydride (1.3 equiv.)
was added. After a further 24 h the reaction had proceeded to completion and was concenfrated in vacuo, partitioned between dichloromethane (100 mL) and sat. aq. NaHCO3 (100 mL). The aqueous layer was further extracted with dichloromethane (4 x 100 mL) and the combined organic layers were dried over magnesium sulfate and evaporated to dryness. Purification by column chromatography over silica gel using dichloromethane:methanol:ammonium hydroxide (78:19:3) as the eluent to afford a brown solid. The tri maleate salt was then formed by standard methods to give Nl-4-(4-amino-7-{4-[l-(l-methylpiperid-4-yl)piperidyl]-7H-pyrrolo[2, 3-d]pyrimidin-5-yl})-2-fluorophenyl-4-fluoro-l -benzenesulfonamide tri-maleate as a brown solid (45 mg, 75%), m/z 582 (MH+) and RP-HPLC (5 to 85 % CH3CN in 0.1 N aqueous ammonium acetate over 20 min at 1 mL/min using a Waters Delta pack 5m C18, 300A, 150 x 3.9 mm column) = 10.658 min., 95%.

Example 317: Nl-4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-methoxyphenylbenzamide
a) To a solution of 4-chloro-5-iodo-7H-pynolo[2,3-d]pyrimidine (25.0 g, 0.09 mol), l,4-dioxaspiro[4.5]decan-8-ol (35.8 g, 0.0267 mol) and triphenylphosphine (46.7 g, 0.178 mol) in TΗF (1.2 L) was added diethylazodicarboxylate (30.9 g, 0.178 mol) under nitrogen. The solution was stined for 20 hr and the majority of solvent was then evaporated (250 mL remaining). EtOAc (450 mL) was then added and the resulting solid was filtered, washed with EtOAc (2 x 50 mL) and dried in vacuo to give 4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-5-iodo-7H-pynolo[2,3-cTlpyrimidine (22.5 g, 60%) as a cream solid. Η NMR (d6 DMSO, 400 MHz) 8.64 (IH, s), 8.10 (IH, s), 4.74 (IH, m), 3.90 (4H, m), 2.12 (2H, m), 1.91 (2H, m), 1,71-1.83 (4H, m). Rf in 1:4 EtOAc : heptane = 0.12.

b) A solution of tert-butyl N-[2-methoxy-4-(4,4,5,5-teframethyl-l,3,2-dioxaborolan-2-yl)phenyl]carbamate (8.2 g, 23.5 mmol), 4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-5-iodo-7H-pynolo[2,3-_f!pyrimidine (6.57 g, 15.7 mmol), tetrakistriphenylphosphinepalladium (1.1 g, 0.93 mmol), sodium carbonate (4.16 g, 39.2 mmol) in dimethoxyethane (200 mL) and water (100 mL) was heated at 80°C for 20 hr under nitrogen. The resulting solution was cooled to room temperature and partitioned between EtOAc (300 mL) and water (100 mL). The aqueous layer was exfracted with EtOAc (3 x 150 mL) and the combined organics were washed with water (1 x 150 mL). The organics were dried (sodium sulphate), filtered and evaporated to leave a solid. On attempting to dissolve in EtOAc/heptane (1:4), a cream solid (2.5 g) crashed out. The filtrate was adsorbed onto silica and purified by flash silica gel column chromatography using 10:1 heptane:EtOAc , 4:1 heptane : EtOAc , 1:1 heptane:EtOAc and 4:1 EtOAc:heptane. The appropriate fractions were combined to give a white solid which was triturated with heptane/EtOAc (5:1) to give tert-butyl N-4-[4-chloro-7-(l ,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-c |pyrimidin-5-yl]-2-methox3φhenylcarbamate
as a solid (3.2 g), combined yield is 71%.
Η ΝMR (d6 DMSO, 400 MHz): 8.66 (IH, s), 7.93 (2H, m), 7.74 (IH, m), 7.19 (IH, s), 7.07 (IH, d), 4.81 (IH, m), 3.93 (4H, m), 3.91 (3H, s), 2.18 (2H, m), 1.99 (2H, m), 1.79 (4H, m), 1. 48 (9H, s). HPLC ( conditions: 5 to 95% CH3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t_ = 21.24 min, 100%>.

c) tert-Butyl N-4-[4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3- ^pyrimidin-5-yl]-2-methoxyphenylcarbamate (5.7 g, 0.011 mol), cone, ammonia solution (100 mL) and dioxan (100 mL) were heated in a pressure vessel for 20 hr at 120°C. The solvent was evaporated and the residue reconstituted in EtO Ac/water (250 mL/100 mL). The organic layer was separated , dried (sodium sulphate), filtered and evaporated to give a solid which by HPLC (conditions: 5 to 95%>

CH3CN in 0.1 N aqueous ammonium acetate over 20 min.) was observed to be a 2:1 mixture of tert-butyl N-4-[4-amino-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3- -f]pyrimidin-5-yl]-2-methoxyphenylcarbamate and 5-(4-amino-3-methoxyphenyl)-7- (l,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-t ]pyrimidin-4-__mine. The mixture was dissolved in acetone (200 mL) and ΗC1 (5Ν, 100 mL) was added dropwise over 0.5 hr. The resulting solution was stined at room temperature overnight and the solvent was then evaporated. The acidic solution was basified with 2N NaOΗ (ice-cooling) and exfracted with EtOAc (3 x 150 mL). The combined organics were washed with water (2 x 100 mL). During the extraction process a solid precipitated. This solid was filtered and triturated in hot EtO Ac/MeOΗ. The insolubles were filtered, the filfrate evaporated and then resulting solid triturated with diethylether/ethyl acetate to give a yellow solid. The organic layers from the original extraction were dried ' (sodium sulphate), filtered and evaporated. The resulting solid was triturated with diethyl ether/ethyl acetate (5:1) and filtered to give 4-[4-amino-5-(4-amino-3- methoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-7-yl]-l-cyclohexanone as a yellow solid. (2.3 g, combined yield = 78%). Η NMR (d6 DMSO, 400 MHz): 8.17 (IH, s), 7.32 (IH, s), 6.88 (IH, s), 6.77QH, m), 6.73 (IH, m), 6.71 (IH, m), 6.07 (2H, bs), 5.14 (IH, m), 3.81 (3H, s), 2.72 (2H, m), 2.35 (4H, m), 2.18 (2H, m). HPLC: ( 5 to 95%> CH3CN in 0.1 N aqueous ammonium acetate over 20 min.) t. = 11.24 min , 95%

d) To a solution of 4-[4-amino-5-(4-amino-3-methoxyphenyl)-7H-pynolo[2,3- -f]pyrinfidin-7-yl]-l-cyclohexanone (0.105 g, 0.3 mmol) in pyridine (2 mL) and dichloromethane (5 mL) was added benzoyl chloride (63 mg, 0.45 mmol) in dichloromethane (1 mL) at 0°C under nitrogen. The solution was stined at 0°C for 2 hr and then quenched with water (5 mL). ΗC1 (IN, 40 mL) was added and the aqueous layer extracted with dichloromethane (3 x 25 mL). The combined organic layers were washed with water (1 x 30 mL). The organic layer was dried (sodium sulphate), filtered and evaporated to leave an oil which was purified by flash silica gel column chromatography using 2%-10% MeOH/EtOAc as eluent to give Nl-4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3-- ]pyrimidin-5-yl]-2-methoxyphenylbenzamide as a white solid (0.130 g, 96%). M.pt 234-237°C. Rf in 9 : 1 EtOAc : MeOΗ = 0.30. ΗPLC: ( 5 to 95% CΗ3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t, = 14.82 min , 96%. Η NMR (d6 DMSO, 400 MHz): 9.43 (IH, s), 8.19 (IH, s), 7.94 (3H, m), 7.59 (4H, m), 7.18 (IH, s), 7.06 (IH, d, J= 8 Hz), 6.18 (2H, bs), 5.20 (IH, m), 3.92 (3H, s), 2.76 (2H, m), 2.35 (4H, m), 2.22 (2H, m).

Example 318: Benzyl N-4-[4-an__ino-7-(4-oxocyclohexyl)-7H-pyπolo[2,3-d]pyrimidin-5-yl]-2-methoxyphenylcarbamate

To a solution of give 4-[4-amino-5-(4-amino-3-methoxyphenyl)-7H-pynolo[2,3-rf]pyrimidin-7-yl]-l-cyclohexanone (0.40 g, 1.15 mmol) in pyridine (5 mL) and dichloromethane (10 mL) was added benzylchloroformate (0.29 g, 1.73 mmol) at -5°C under nitrogen. The solution was warmed to 0°C and stined for 1 hr. The reaction was quenched with water (5 mL) and the solvent evaporated. The residue was partitioned between EtOAc and water (100 mL each) and the aqueous layer was extracted with EtOAc (3 x 50 mL). The combined organics were dried (sodium sulphate), filtered and evaporated to leave a solid which was triturated with EtOAc/Et,O to give benzyl N-4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3-<i]pyrimidin-5-yl]-2-methoxyphenylcarbamate (0.28 g) as a yellow solid. M.pt 175-176°C. Rf in 9:1 EtOAc : MeOΗ = 0.24. ΗPLC: ( 5 to 95% CΗ3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t- = 16.69 min , 98%>. lH NMR (d6 DMSO, 400 MHz): 8.64 (IH, s), 8.17 (IH, s), 7.75 (IH, d, J= 8.4 Hz), 7.50 (IH, s), 7.36 (5H, m), 7.10 (IH, s), 7.02 (IH, d, J= 8 Hz), 6.15 (2H, bs), 5.19 (3H, m), 3.81 (3H, s), 2.72 (2H, m), 2.35 (4H, m), 2.22 (2H, m).

Example 319: Cis- benzyl N-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}2-methoxyphenyl)carbamate tri-maleate and
Trans- benzyl N-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-methoxyphenyl)carbamate tri-maleate
To a solution of benzyl N-4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3-_i]pyrimidin-5-yl]-2-methoxyphenylcarbamate (0.83 g, 1.74 mmol), Ν-methylpiperazine (0.52 g, 5.22 mmol) and glacial acetic acid (0.31 g, 5.22 mmol) in dichloroethane (100 mL) under nitrogen was added sodium triacetoxyborohydride (0.55 g, 2.61 mmol) portionwise. The solution was stined for 6 hr and then quenched by the addition of sodium hydroxide (2Ν, 20 mL). The organic layer was separated and the aqueous layer was exfracted with dichloromethane (2 x 50 mL). The combined organics were washed with brine (1 x 50 mL), dried (sodium sulphate), filtered and evaporated to leave an oil which was purified by flash silica gel column chromatography using EtO Ac, 9:1 EtO Ac :MeOΗ, CΗ2C12 and 9:1 CH2Cl2:MeOH to give in F20-25 an oil (480 mg). This oil was dissolved in ethyl acetate and treated with maleic acid (280 mg) in ethyl acetate. The resulting solid was filtered under a stream of nifrogen and dried in vacuo for 4 hr to give Cώ-benzyl N-(4-{4-amino-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo[2,3-cT)pyrimidin-5-yl}-2-methoxyphenyl)carbamate tri-maleate salt (580 mg) as a cream solid. M.pt. 158°C (dec.) Η ΝMR (d6 DMSO, 400 MΗz):8.74 (IH, s), 8.27 (IH, s), 7.78 (IH, d), 7.35-7.77 (5H, m), 7.10 (IH, s), 7.04 (IH, s), 6.16 (6H, s), 5.17 (2H, s), 4.74 (IH, m), 3.82 (3H, s), 3.23 (5H, m), 2.78 (3H, s), 2.51 (3H, m), 2.41 (IH, s), 2.09 (4H, m), 1.70 (4H, m). HPLC: ( 5 to 95% CH3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t. = 13.30 min , 94%>.
F28-45 gave a glassy foam (186 mg) which was dissolved in in ethyl acetate (10 mL) and treated with maleic acid (114 mg) in ethyl acetate (3 mL). The resulting solid was filtered under nifrogen and dried in vacuo for 4 hr to give trans- benzyl N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_flpyrimidin-5-yl}-2-methoxyphenyl)carbamate tri-maleate salt (250 mg) as a cream solid. M.pt

146-148°C. ΗPLC: ( 5 to 95% CΗ3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t- = 13.54 min , 94.6%. Η NMR (d6 DMSO, 400 MHz): 8.72 (IH, s), 8.25 (IH, s), 7.77 (IH, d), 7.51 (IH, s), 7.35 (5H, m), 7.10 (IH, s), 7.04 (IH, d), 6.16 (6H, s), 5.17 (2H, s), 4.59 (IH, m), 3.86 (3H, s), 2.70-3.10 (11H, m), 2.50 (3H, s), 1.97 (6H, m), 1.56 (2H, m).

Exxample 320: Trans-Nl-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-methoxyphenyl)benzamide
To a solution of Nl-4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3-_/]pyrimidin-5-yl]-2-methoxyphenylbenzamide (1.2 g, 2.66 mmol), Ν-methylpiperazine (0.80 g, 7.98 mmol) and glacial acetic acid (0.48 g, 7.98 mmol) in dichloroethane (150 mL) under nitrogen was added sodium triacetoxyborohydride (0.85 g, 3.99 mmol) portionwise. The solution was stined at room temperature overnight and then quenched by the addition of sodium hydroxide (2 Ν, 20 mL). The aqueous layer was exfracted with dichloromethane (3 x 50 mL) and the combined organics were dried (sodium sulphate), filtered and evaporated to leave a solid which was purified by flash silica gel column chromatography using dichloromethane then 5%> MeOΗ/dichloromethane to 20%> MeOΗ/ dichloromethane in 5%> increments. F23-36 were combined and evaporated to give a cream solid (0.11 g) which was dissolved in EtOAc (10 mL) and treated with a solution of maleic acid ( ) in EtOAc (5 mL). The resulting fine solid was filtered under a stream of mfrogen to give trαn_?-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-cTlpyrimidin-5-yl}-2-methoxyphenyl)benzamide (0.108 g) as a cream solid. lΗ ΝMR (d6 DMSO, 400 MHz): 9.48 (IH, s), 8.28 (IH, s), 7.97 (3H, m), 7.53-7.63 (4H, m), 7.18 (IH, s), 7.08 (IH, d), 6.85 (IH, bs), 6.16 (6H, s), 4.61 (IH, m), 3,92 (3H, s), 2.70-3.11 (11H, m), 2.01 (7H, m), 1.58 (2H, m). HPLC/MS (Column = Pecosphere 3 C,s 3 micron, conditions = 100%> lOOmM ammonium acetate to 100%> acetonitrile over 5 min), tr = 1.83 min, MET = 540.8

Example 321 : Cis-Νl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-methoxyphenyl)-3-phenylpropanamide
and Trans- Nl -(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-methoxyphenyl)-3-phenylpropanamide
a) To a solution of 4-[4-an__ino-5-(4-amino-3-methoxyphenyl)-7H-pynolo[2,3-J]pyrimidin-7-yl]-l-cyclohexanone (0.8 g, 2.3 mmol) in pyridine (13 mL) and dichloromethane (32 mL) at 0°C was added hydrocinnamoylchloride (0.57 g, 3.4 mmol) in dichloromethane (5 mL) under nitrogen. The solution was stined at 0°C for 2 hr, warmed to room temperature and quenched by addition of saturated aqueous citric acid solution (50 mL). The organic layer was washed with saturated aqueous citric acid solution (2 x 50 mL), dried (sodium sulphate), filtered and evaporated to leave Nl - {4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3- _ ]pyrimidin-5-yl]-2-methoxyphenyl}-3-phenylpropanamide (1.0 g, 92%> crude) as a brown foam. Η ΝMR (d6 DMSO, 400 MΗz):9.17 (IH, s), 8.18 (IH, s), 8.06 (IH, d), 7.51 (IH, s), 7.18-7.29 (6H, m), 7.09 (IH, m), 6.99 (IH, d), 6.21 (2H, bs), 5.18 (IH, m), 3.88 (3H, s), 1.99 - 2.93 (12H, m). HPLC: (5 to 95% CH3CΝ in 0.1 N aqueous ammonium acetate over 20 min.) t. = 14.48 min , 92.2%>.

c) To a solution of 92%o pure Nl - {4-[4-amino-7-(4-oxocyclohexyl)-7H-pynolo[2,3- < |pyrimidin-5-yl]-2-methoxyphenyl}-3-phenylρropanamide (1.0 g, 2.1 mmol), Ν-methylpiperazine (0.63 g, 6.3 mmol), acetic acid (0.38 g, 6.3 mmol) in dichloroethane (100 mL) was added sodium triacetoxyborohydride (0.67 g, 3.15 mmol) portionwise under nitrogen. The solution was stined for 20 hr and then quenched by the addition of saturated aqueous sodium bicarbonate solution (50 mL). The aqueous layer was extracted with dichloromethane (3 x 50 mL), dried (sodium sulphate), filtered and evaporated to leave a sludge which was purified by flash silica gel column chromatography using dichloromethane to 50%

MeOΗ/dichloromethane in 10% increments. F84-96 were combined and evaporated to leave ct-f-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-< pyriιrnά^-5-yl}-2-methoxyphenyl)-3-phenylprop_mamide (0.26 g) as a cream foamy glass. ΗPLC: (5 to 95%> CΗ3CΝ in 0.1 N aqueous ammomum acetate over 20 min.) tr = 12.65 min , 95.2%.lH NMR (d6 DMSO,

400 MHz): 9.17 (IH, s), 8.14 (IH, s), 8.05 (IH, d ), 7.28 (5H, m), 7.18 (IH, m), 7.10 (IH, s), 6.99 (IH, d), 6.11 (2H, bs), 4.67 (IH, m), 3.88 (3H, s), 2.90 (2H, m), 2.73 (2H, m), 2.50 (7H, m), 2.28 (3H, s), 2.06 (3H, m), 1.71 (2H, m), 1.55 (2H, m). F121-138 were combined and evaporated to leave trans- Nl-(4-{4- amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-cf]pyrimidin-5-yl}- 2-methoxyphenyl)-3-phenylpropanamide (0.11 g) as a white solid. ΗPLC: (5 to

95%o CΗ3CΝ in 0.1 Ν aqueous ammonium acetate over 20 min.) X = 12.61 min , 96.2%. Η ΝMR (d6 DMSO, 400 MΗz):9.16 (IH, s), 8.13 (IH, s), 8.04 (IH, d), 7.44 (IH, s), 7.29 (4H, m), 7.18 (IH, m), 7.09 (IH, s), 6.97 (IH, d), 6.11 (2H bs), 4.53 (IH, m), 3.88 (3H, s), 2.93 (2H, m), 2.71 (2H, m), 2.50 (4H, m), 2.30 (5H, m), 2.14 (3H, s), 1.89 (6H, m), 1.46 (2H, m).

General Procedure for Substituted Pynolopyrimidine Aryl sulfonamides are as follows:
A 0.19 M solution of 5-(4-amino-3-fluorophenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine in pyridine was added one equivalent of substituted aryl sulfonyl chloride. The mixture was heated to 45o C while being shaken in an Incubator Shaker for 24h. The reaction mixture was purified by using mass actuated preparative RP-HPLC (Micromass/Gilson, Hypersil BDS C18, 5u, 100x21.2mm; 100-100% ammonium acetate (0.05 M, pH 4.5)-acetonitrile over 12.5 min, 25 mL/min).

Compounds synthesized by the above procedure include:

Name HPLC rt m/z
min
Example 322: Trans-N-l-(4-{4-amino-7-[4-(4- 3.18 648.39
methylpiperazino)cyclohexyl] -7H-pyπolo [2,3 -d]ρyrimidin-5-yl} -2-fluorophenyl)-2-(trifluoromethoxy)- 1 -benzenesulfonamide trimaleate Example 323: Trans-N- 1 -(4- {4-amino-7-[4-(4- 3.14 604.03 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-5-chloro-2-thiophenesulfonamide benzenesulfonamide
trimaleate

Example 324: Trans-N- 1 -(4- {4-amino-7-[4-(4- 3.07 616.1 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-chloro-4-fluoro- 1 -benzenesulfonamide benzenesulfonamide
trimaleate

Example 325: Trans-N-l-(4-{4-amino-7-[4-(4- 3.39 632.12 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3-dichloro- 1 -benzenesulfonamide trimaleate

Example 326: cis-N-l-(4-{4-amino-7-[4-(4- 2.82 616.2 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-chloro-4-fluoro-1 -benzenesulfonamide trimaleate

Example 327: cis-N- l-(4-4-amino-7-[4-(4- 2.66 600.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl-2-fluorophenyl)-2,6-difluoro- 1 -benzenesulfonamide trimaleate

Example 328: Trans-N- 1 -(4- {4-amino-7-[4-(4- 2.53 600.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,6-difluoro-l-benzenesulfonamide trimaleate
Example 329: Trans- N-4-(4-{4-amino-7-[4-(4- 2.63 622.1 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2, 1 ,3-benzothiadiazole-4-sulfonamide trimaleate
Example 330: Trans-N- 1 -(4- {4-amino-7-[4-(4- 2.87 618.1 methylpiperazino)cyclohexyl] -7H-pyπolo [2,3 -d]pyrimidin-5-yl} -2-fluorophenyl)-2,3,4-trifluoro- 1 -benzenesulfonamide trimaleate
Example 331: Cis-N-l-(4-{4-amino-7-[4-(4- 3.13 609.1 methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}-2-fluorophenyl)-2-nitro-l-benzenesulfonamide trimaleate
Example 332: Cis-N-l-(4-{4-amino-7-[4-(4- 2.89 582.1 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-fluoro-l-benzenesulfonamide trimaleate
Example 333: Cis-N-l-(4-{4-amino-7-[4-(4- 3.4 668 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,4,6-trichloro-l-benzenesulfonamide trimaleate
Example 334: Cis-N-l-(4-{4-amino-7-[4-(4- 3.04 632.1 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]ρyrimidin-5-yl} -2-fluorophenyl)-2,6-dichloro- 1 -benzenesulfonamide trimaleate
Example 335: Cis-N-l-(4-{4-amino-7-[4-(4- 2.94 598.1 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2-chloro- 1 -benzenesulfonamide trimaleate Example 336: Cis-N-l-(4-{4-amino-7-[4-(4- 2.76 582.1 methylpiρerazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)- 3-fluoro- 1 -benzenesulfonamide dimaleate
Example 337: cis-N-l-(4-{4-amino-7-[4-(4- 3.01 604.3 methylρiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-5-chloro-2-thiophenesulfonamide dimaleate

Example 338: Cis-N-l-(4-{4-amino-7-[4-(4- 3.38 718.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-bromo-2,5-difluoro- 1 -benzenesulfonamide trimaleate
Example 339: Cis-N-l-(4-{4-amino-7-[4-(4- 2.98 616.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-3-chloro-4-fluoro-1 -benzenesulfonamide trimaleate
Example 340: Cis-N-l-(4-{4-amino-7-[4-(4- 3.02 690.3 methylρiper__zino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl}-2-fluoroρhenyl-2-iodo-l-benzenesulfonamide trimaleate
Example 341: cis-N-l-(4-{4-amino-7-[4-(4- 3.22 648.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-(trifluoromethoxy)- 1 -benzenesulfonamide trimaleate

Example 342: Cis-N-l-(4-{4-amino-7-[4-(4- 2.97 600.3 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3-dichloro- 1 -benzenesulfonamide trimaleate
Example 343: Cis-N-l-(4-{4-amino-7-[4-(4- 3.12 612.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} -2-fluorophenyl)-2-chloro-6-methyl- 1 -benzenesulfonamide trimaleate Example 344: Cis-N-l-(4-{4-amino-7-[4-(4- 3.02 623.2 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-chloro-4-cyano-1 -benzenesulfonamide trimaleate
Example 345: Cis-N-l-(4-{4-amino-7-[4-(4- 3.08 618.3 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3,4-trifluoro-l-benzenesulfonamide trimaleate
Example 346: Cis-N-l-(4-{4-amino-7-[4-(4- 2.98 600.3 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-3 ,4-difluoro- 1 -benzenesulfonamide trimaleate
Example 347: Cis-N-l-(4-{4-amino-7-[4-(4- 3.13 660.2 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-4-bromo-2-fluoro- 1 -benzenesulfonamide trimaleate
Example 348: Cis-N-l-(4-{4-amino-7-[4-(4- 3.16 648.1 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-5-bromo-2-thiophenesulfonamide trimaleate
Example 349: Cis-N-l-(4-{4-amino-7-[4-(4- 3.09 632.1 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,4-dichloro-l-benzenesulfonamide trimaleate
Example 350: Cis-N-l-(4-{4-amino-7-[4-(4- 3.41 668.1 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3 ,4-trichloro- 1 -benzenesulfonamide trimaleate
Example 351: Cis-N-l-(4-{4-amino-7-[4-(4- 3.29 683.9 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-3-bromo-5-chloro-2-thiophenesulfonamide trimaleate Example 352: Cis- N-4-(4-{4-amino-7-[4-(4- 2.73 622.1 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,l,3-benzothiadiazole-4-sulfonamide trimaleate
Example 353: cis- N-4-(4-{4-amino-7-[4-(4- 2.8 606.1 methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2, 1 ,3 -benzoxadiazole-4-sulfonamide trimaleate
Example 354: Cis-N-l-(4-{4-amino-7-[4-(4- 3.18 638 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,5-dichloro-l-thiophenesulfonamide trimaleate
Example 355: cis- N-4-(4-{4-amino-7-[4-(4- 2.84 640.2 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-(7-chloro-2,l,3-benzoxadiazole)-4-sulfonamide trimaleate
Example 356: Cis- N-4-(4-{4-amino-7-[4-(4- 2.89 636.2 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(7-methyl-2, 1,3-benzothiadiazole)-4-sulfonamide trimaleate
Example 357: Cis- N-4-(4-{4-amino-7-[4-(4- 2.82 636.2 methylpiperazino)cyclohexyl]-7H-pynolo [2,3 -d]pyrimidin-5-yl} -2-fluorophenyl)-(5-methyI-2, 1,3-benzothiadiazole)-4-sulfonamide trimaleate
Example 358: Cis- N-4-(4-{4-amino-7-[4-(4- 2.82 656.2 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-(5-chloro-2, 1,3-benzothiadiazole)-4-sulfonamide trimaleate
Example 359: Cis-N-l-(4-{4-amino-7-[4-(4- 3.01 612 methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-3-chloro-2-methyl- 1 -benzenesulfonamide trimaleate Example 360: Cis-N-l-(4-{4-amino-7-[4-(4- 2.81 644.2
methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2-bromo-l-benzenesulfonamide trimaleate
Example 361: Cis-N-l-(4-{4-amino-7-[4-(4- 3.29 758.1
methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,5-dibromo-356-difluoro-1 -benzenesulfonamide trimaleate
Example 362: Cis-N-l-(4-{4-amino-7-[4-(4- 2.77 632
methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)-2,3-dichloro- 1 -benzenesulfonamide trimaleate
Example 363: Cis-N-l-(4-{4-amino-7-[4-(4- 2.73 623.2
methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} -2-fluorophenyl)- (2-nifrophenyl)methanesulfonamide trimaleate

General Synthesis
Method (a)
A mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidine-7-yl]- 1-cyclohexanone (1.0 g, 2.51 mmol), the appropriate amine (7.54 mmol) and acetic acid (0.45 g, 7.54 mmol) in 1,2-dichloroethane (50 mL) was stined at ambient temperature under an atmosphere of nitrogen for 30 minutes. Sodium
triacetoxyborohydride (0.69 g, 3.26 mmol) was added and the mixture stined at ambient temperature for 18 hours. Water (20 mL) and sodium bicarbonate (1.26 g, 15.1 mmol) were added to the mixture and stined for one hour. The mixture was then filtered through a pad of celite and the pad was washed with dichloromethane (75 mL). The organic layer was extracted from the filfrate, dried over magnesium sulfate, filtered and evaporated to dryness under reduced pressure. The cis and frans isomers were purified by flash chromatography on silica gel using a
methanohdichloromethane gradient.

Method (b)
Where appropriate the salts were made as follows.
The above amine (0.909 mmol) was dissolved in warm ethyl acetate (100 mL) then maleic acid (0.32 g, 2.73 mmol) in ethyl acetate (30 mL) was added. The resulting salt formed an oily residue on the bottom and sides ofthe flask. The supernatant was poured off and the residue was dissolved in water and lyophilized to give the salt

Method (c)
Guanidines were made as follows. The amine (0.536 mmol) was dissolved in DMF (5 mL) and cooled to -5°C and then 1-H pyrazole-1-carbonamide (95 mg, 0.644 mmol) followed by dusopropylethylamine (208 mg, 1.6 mmol) were added. The reaction mixture was allowed to warm to rt over 16h and then concentrated in vacuo. The reaction was partititoned between water (10 mL) and ethyl acetate (10 mL). The aqueous phase was lyophilized and purified by RP-HPLC.
HPLC protocols:
1. RP-HPLC - Hypersil HyPurity Elite C18, 5 mm, 200A, 250 x 4.6 mm; 25-100% acetonitrile-0.1 M ammomum acetate over 15 min, lml/min.
2. RP-HPLC - Hypersil HyPurity Elite C18, 5 mm, 200A, 250 x 4.6 mm; 5-100% acetonitrile-0.1 M ammonium acetate over 15 min, lml/min.
It should be appreciated that protecting group chemistry is used where approriate.

The following examples were prepared using the methods described above:
Name Synthetic HPLC-RT m/z Additional
method (Min) (MhT) chemistry
(Protocol)
Example 364: Cis-4-{4-[4- C 14.56 511.7
amino-5-(4-ρhenoxyρhenyl)- (2)
7H-pynolo [2,3 -d]pyrimidin-7-yl]cyclohexyl}-l-piperazinecarboximidamide Example 365: Trans-4-{4-[4- 14.25 511.7
amino-5-(4-phenoxyphenyl)- (2)
7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl} - 1 -piperazinecarboximidamide

Example 366: Trans-7-(4- a, b 8.55 519.6
{methyl[2-(2- (2)
pyridyl)ethyl] amino } cyclohexy
l)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]ρyrimidin-4-amine trimaleate

Example 367: Cis-3-({4-[4- 10.21 472.6 Made by amino-5-(4-phenoxyphenyl)- (2) hydrolysis
7H-pynolo[2,3-d]pyrimidin-7- of the ester yl]cyclohexyl} amino)propanoi
c acid

Example 368: 3-({4-[4-amino- 6.33 472.6 Made by
5 -(4-phenoxyphenyl)-7H- (1) hydrolysis pynolo [2,3 -d]pyrimidin-7- of the ester yl]cyclohexyl} amino)propanoi
c acid

Example 369: Ethyl cis-3-({4- a, b 10.42 500.6
[4-amino-5-(4- (1)
phenoxyphenyl)-7H-pynolo [2,3 -d]pyrimidin-7-yl]cyclohexyl} amino)propanoa
te dimaleate

General Synthesis

Method (d)
To a solution of sodium hydride (22 mg, 0.553 mmol) in THF (2 mL) was added the appropriate phosphonate (0.553 mmol) at 0°C and the resultant mixture was stined at this temperature for 20 min and then at ambient temperature for 10 min. The reaction mixture was cooled to 0°C and 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimin-7-yl]cyclohexanone (200 mg, 0.503 mmol) added in THF (10 mL) and the resultant mixture allowed to warm to ambient temperature and stined for 16h. The solvents were removed in vacuo and the residue partitioned between ethyl acetate (10 mL) and water (10 mL). The aqueous layer was further extracted into ethyl acetate (3 x 5 mL) and the combined organics were washed with water (3 x 5 mL), dried (MgSO4) and concentrated in vacuo. Purification by flash column chromatography on silica gel (for intermediates) or RP-HPLC (for final compounds) gave the desired compound.

Method (e)
Hydrogenations were carried out as follows. A mixture ofthe alkene, (0.068 mmol) and 10%) Pd/C (12 mg) in ethanol (18 mL) was stined under hydrogen (4 arm) for 14h. The solids were removed by filtration and the filtrate concenfrated in vacuo. Purification by RP-HPLC gave the final compound.

Method (f)
Lithium aluminum hydride reuctions were carried out as follows. A mixture ofthe subsfrate (0.19 mmol), lithium aluminum hydride (40 mg, 1.07 mmol) in THF (5 mL) was stined at room temperature for 16h. Fieser work up followed by purification by RP-HPLC gave the desired compound.
HPLC conditions : RP-HPLC Pecosphere3 C18, 33 x 4.6 mm, 3m column; 0-100% acetonitrile-0.1 M ammonium acetate over 5 min, flow 4ml/min.
It should be appreciated that protecting group chemistry is used where approriate.

Name Synthetic HPLC- m/z Additional
Method RT (MH+) Chemistry
(Min) Exa ple 370: {4-[4-Amino-5-(4- 3.1 422.5
phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyliden}
methyl cyanide

Example 371: tert-Butyl 2- 3.97 497.1
[4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl] cyclohexy liden} acetate
Example 372: Ethyl 2- 3.56 469.0
[4-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-d]pyrimidin-7-yl]cyclohexyliden} acetate
Example 373: 2-[4-[4-Amino-5- 2.69 441.5 Made by (4-phenoxyphenyl)-7H- hydrolysis pynolo[2,3-d]pyrimidin-7- of the ethyl yl]cyclohexyliden} acetate ester

Example 374: 7-[4-(2- 2.11 428.5 Made by aminoethyl)cyclohexyl]-5-(4- lithium phenoxyphenyl)-7H-pynolo[2,3- aluminum d]pyrimidin-4-amine hydride
reduction of
the
unsaturated
cyanide

Example 375: 2-{4-[4-amino-5- 2.64 443.5 Made by (4-phenoxyphenyl)-7H- hydrogenatio pynolo [2,3 -d]pyrimidin-7- n
yl]cyclohexyl} acetic acid of the
unsaturated
acid

Example 376: Trans-5- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl}-2-phenoxybenzonitrile bisacetate

a) 5-bromo-phenoxybenzonitrile A mixture of 4-bromo-2-fluorobenzonitrile (5.0 g, 25 mmol), phenol (4.7 g, 50 mmol) and potassium carbonate (6.9 g, 50 mmol) in dimethylsulfoxide (30 mL) was heated at 100°C for 18 hours. The mixture was cooled and water (200 mL) was added. The mixture was extracted with dichloromethane (2 x 50 mL). The combined organic solutions were washed with 15% aqueous sodium hydroxide (50 mL), saturated aqueous sodium bicarbonate (50 mL), dried over magnesium sulfate and filtered. The filtrate was concentrated under reduced pressure to give 5-bromo-phenoxybenzonitrile (7.25 g, quant.): Η NMR (DMSO-dδ, 400 MHz) δ 8.20 (d, IH), 7.84 (dd, IH), 7.47 (t, 2H), 7.30 (t, IH), 7.20 (dd, 2H), 6.87 (d, IH); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammonium acetate over 10 min, 1 ml/min) tr 12.50 min.

b) 2-phenxoy-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolane-2-yl)benzonitrile
A mixture of 5-bromo-phenoxybenzonitrile (2.0 g, 7.22 mmol), diboron pinacol ester (2.2 g, 8.66 mmol), potassium acetate (2.12 g, 21.7 mmol) and [1,1'-bis(diρhenylphosphino)fenocene]dichloropalladium (II) complex with
dichloromethane (1:1) (0.35 g, 0.49 mmol) in NN-dimethylformamide (40 mL) was heated at 85°C for 20 hours then cooled and the solvents evaporated under reduced pressure. The residue was triturated with dichloromethane (40 mL), then filtered through a pad of celite and the filtrate concentrated to give an oil which was purified by flash chromatography on silica gel using heptane/ethyl acetate (9:1) as an eluent to give 2-phenoxy-5-(4,4,5,5-tetramethyl-l ,3,2-dioxaborolane-2-yl)benzonitrile (1.56 g, 67%): Η ΝMR (DMSO-d6, 400 MHz) δ 8.01 (s, IH), 7.88 (d, IH), 7.49 (t, 2H), 7.32 (t, IH), 7.22 (d, 2H), 6.90 (d, IH), 1.29 (s, 12H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%>-100%> acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t- 13.85 min.

c) tr «5-5-(4-chloro-7-(4-(4-methylpiperazino)cyclohexyl)-7H-pynolo[2,3-J]pyrimidin-5-yl)-2-phenoxybenzonitrile
A mixture of trαn.f-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-.f]pyrimidine (500 mg, 1.09 mmol), 2-phenxoy-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolane-2-yl)benzonitrile (425 mg, 1.31 mmol), sodium carbonate monohydrate (325 mg, 2,62 mmol) and
tetrakis(triphenylphosphine)palladium(0) (75 mg, 0.065 mmol) in ethylene glycol dimethyl ether (8 mL) and water (4 mL) was heated at 85°C for 18 hours. The mixture was cooled and evaporated under reduced pressure then partitioned between water (10 mL) and dichloromethane (25 mL). The combined organic solutions were dried over magnesium sulfate and then filtered. The filtrate was concenfrated to give a material which was then purified by flash chromatography on silica gel using dichloromethane/methanol (9:1) as an eluent to give tra«5-5-(4-chloro-7-(4-(4-mexhylpiperazino)cyclohexyl)-7H-pynolo[2,3-_i]pyrimidin-5-yl)-2-phenoxybenzonifrile: Η NMR (DMSO-d6, 400 MHz) δ 8.11 (s, IH), 8.07 (d, IH), 7.5-7.55 (m, 3H), 7.01 (d, IH), 4.71 (m, IH), 2.2-2.6 (m, 9H), 2.17 (s, 3H), 1.8-2.1 (m, 6H), 1.4-1.6 (m, 2H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100%o acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 9.20 min.

d) trα«5-5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-J]pyrimidin-5-yl} -2-phenoxybenzonitrile bisacetate
A mixture of tra«5-5-(4-chloro-7-(4-(4-methylpiperazino)cyclohexyl)-7H-pynolo[2,3-_ [pyrimidin-5-yl)-2-phenoxybenzonifrile (150 mg) in 1,4-dioxane (25 mL) and 30%> aqueous ammonium hydroxide (25 mL) was heated at 120°C in a sealed tube for 18 hours then cooled and evaporated under reduced pressure. The residue was purified by preparative RP-ΗPLC to give 5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-5-yl}-2-phenoxybenzonifrile bisacetate: Η NMR (DMSO-d6, 400 MHz) δ 8.14 (s, IH), 7.91 (s, IH), 7.70 (d, IH), 7.55 (s, IH), 7.48 (t, 2H), 7.27 (t, IH), 7.20 (d, 2H), 7.06 (d, IH), 6.27 (bs, 2H), 4.55 (m, IH), 2.53 m, 4H), 2.36 (m, 5H), 2.17 (s, 3H), 2.0 (m, 6H), 1.96 (s, 6H); MS MH÷ 508.3; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100%> acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/niin) t-7.27 min.

Example 377: Trans-5- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-J]pyrimidin-5-yl}-2-phenoxybenzamide

A mixture of (50 mg, 0.1 mmol) was dissolved in dimethylsulfoxide (1.6 mL) then treated with 30%) aqueous hydrogen peroxide (1.25 mL) and 15% aqueous sodium hydroxide (1 mL). The mixture was stined at ambient temperature for 18 hours then evaporated and the residue purified by preparative RP-ΗPLC then lyophilized. The material was dissolved in methanol (8 mL) and acetic acid (2 mL) then hydrogenated for 4 hours at atmospheric pressure and ambient temperature in the presence of 10%> Pd-C (15 mg). The mixture was filtered through a pad of celite, evaporated under reduced pressure and then lyophilized to give tr_z«i,-5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-< pyrimidin-5-yl}-2-phenoxybenzamide (10 mg, 20%): Η NMR (DMSO-d6, 400 MHz) δ 8.14 (s, IH), 7.78 (d, IH), 7.65 (s, IH), 7.58 (s, IH), 7.39-7.50 (m, 4H), 7.16 (t, IH), 7.10 (d, 2H), 6.98 (d, IH), 6.15 (bs, 2H), 4.55 (m, IH), 2.2-2.6 (m, 9H), 2.14 (s, 3H), 1.8-2.0 (m, 6H), 1.41-1.48 (m, 2H); MS MET 526.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%>-100%> acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t_ 5.83 min

Example 378: rr_.«_?-5-(3-methoxy-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-c/]pyrimidin-4-amine acetate

a) 4-bromo-2-methoxy- 1 -phenoxybenzene
A mixture of 4-bromoguaiacol (2.23 g, 11 mmol), phenyl boronic acid (4.03 g, 33 mmol), copper(ϋ) acetate (2.18 g, 12 mmol) and 4A powdered molecular sieves (20 g) in dichloromethane (100 mL) was treated with triethylamine (5.5 g, 55 mmol) then stined at ambient temperature for 20 hours. The mixture was filtered, concentrated under reduced pressure and purified by flash chromatography on silica gel using heptane/ethyl acetate (75:25) as an eluent to give 4-bromo-2-methoxy-l- phenoxybenzene (350 mg, 12%):Η NMR (DMSO-_ tf, 400 MHz) δ 7.3-7.4 (m, 3H),

7.15 (d, IH), 7.05 (t, IH), 6.98 (d, IH), 6.85 (d, 2H), 3.77 (s, 3H); RP-HPLC
(Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammonium acetate over 10 min, 1 ml/min) tr 13.12 min.

b) 2-(3-methoxy-4-phenoxyphenyl)-4,4,5,5-tetramethyl-l,3,2-dioxaborolane
The title compound was prepared from 4-bromo-2-methoxy-l- phenoxybenzene and pinacol diboron ester in the same manner as described for 2- phenoxy-5-(4,4,5,5-tetramethyl- 1 ,3,2-dioxaborolane-2-yl)benzonitrile (58% yield) Η NMR (DMSO- β, 400 MHz) δ 7.34 (m, 4H), 7.07 (t, IH), 7.06 (d, IH), 6.87 (d,

2H), 3.77 (s, 3H), 1.31 (s, 12H); TLC (heptane/ethyl acetate 9:1) Rf 0.23.
c) t /. .-4-chloro-5-(3-methoxy-4-phenoxyphenyl)-7-[4- (methylρiperazino)cyclohexyl]-7H-pynolo[2,3-cT|pyrimidine
The title compound (223 mg, 64%>) was prepared from trαras-4-chloro-5- iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-cTlpyrimidme (300 mg,

0.655 mmol) and 2-(3-methoxy-4-phenoxyphenyl)-4,4,5,5-tetramethyl-l,3,2- dioxaborolane (235 mg, 0.727 mmol) in a similar manner to that described for the

' preparation of tr n_f-5-(4-chloro-7-(4-(4-methylpiperazino)cyclohexyl)-7H- pynolo[2,3-_i]pyrimidin-5-yl)-2-phenoxybenzonitrile: Η NMR (DMSO-d6, 400 MHz) δ 8.67 (s, IH), 8.04 (s, IH), 7.32 (m, 4H), 7.12 (d, IH), 7.08 (d, IH), 6.88 (d,

2H), 4.71 (m, IH), 3.80 (s, 3H), 2.2-2.6 (m, 9H), 2.15 (s, 3H), 1.9-2.1 (m, 6H), 1.49

(m, 2H); MS MET 532.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%- 100%) acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 9.58 min.

d) tr_z«_.-5-(3-methoxy-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-_i]pyrimidin-4-amine acetate
A mixture of trαR5-4-chloro-5-(3-methoxy-4-phenoxyphenyl)-7-[4- (methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-(i]pyrimidine (220 mg, 0.414 mmol) in 1,4-dioxane (20 mL) and 30%> aqueous ammonium hydroxide (20 mL) was heated at 120°C in a sealed tube for 18 hours then cooled, concentrated under reduced pressure and purified by preparative RP-ΗPLC to give trαws-5-(3-methoxy-4- phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-- ]pyrimidin-4-amine acetate (122 mg, 58%): Η NMR (DMSO-d6, 400 MHz) δ 8.14 (s, IH), 7.49 (s, IH), 7.32 (t, 2H), 7.22 (s, IH), 7.10 (d, IH), 7.05 (m, 2H), 6.92 (d, 2H), 6.2 (bs, 2H), 4.56 (m, IH), 3.79 (s, 3H), 2.2-2.6 (m, 9H), 2.15 (s, 3H), 1.8-2.05 (m, 6H), 1.90 (s, 6H), 1.45 (m, 2H); MS MH+ 513.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%>-100%> acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 14.77 min.

Example 379: Trans-5- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ^pyrimidin-5-yl}-2-phenoxyphenol

A mixture of trαwj,-5-(3-methoxy-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-J]pyrimidin-4-amine acetate (50 mg, 0.098 mmol) in 48%> aqueous hydrobromic acid (1 mL) was heated at 120°C for 2 hours. The solution was cooled and concentrated under reduced pressure then purified by preparative RP-ΗPLC to give tr «_.-5-{4-amino-7-[4-(4-methylρiperazino)cyclohexyl]-7H-pynolo[2,3-cT]pyrimidin-5-yl}-2-phenoxyphenol (32 mg, 66%):'Η NMR (DMSO-d6, 400 MHz) δ 9.8 (bs, IH), 8.13 (s, IH), 7.39 (s, IH), 7.31 (t, 2H), 7.03 (m, 3H), 6.89 (m, 3H), 6.17 (bs, 2H), 4.55 (m, IH), 2.2-2.6 (m, 9H), 2.15 (s, 3H), 1.8-2.2 (m, 6H), 1.44 (m, 2H); MS MH+ 499.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammonium acetate over 10 min, 1 ml/min) t. 13.47 min.

Example 380: 7 a«-.-5-(3-chloro-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-4-amine

a) 4-bromo-2-chloro- 1 -phenoxybenzene
A mixture of 4-bromo-2-chlorophenol (2.07 g, 10 mmol), phenyl boronic acid (3.66 g, 30 mmol), copper(II) acetate (2.0 g, 1.1 mmol) and 4A powdered molecular sieves (20 g) in dichloromethane (100 mL) was freated with triethylamine (5.0 g, 50 mmol) then stined at ambient temperature for 5 days. The mixture was diluted with heptane (100 mL), then filtered through a bed of celite. The filtrate was
concentrated under reduced pressure to remove dichloromethane. The mixture was extracted with IN hydrochloric acid, 15 wt%> aqueous sodium hydroxide, brine and then dried over magnesium sulfate. The mixture was filtered and the filfrate concentrated under reduced pressure to provide (1.27 g, 45%): lH NMR (DMSO-d6, 400 MHz) δ 7.87 (s, IH), 7.55 (d, IH), 7.40 (t, 2H), 7.17 (t, IH), 6.99 (m, 3H); MS MH+ 283; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) tr 14.42min.

b) 2-(3-chloro-4-phenoxyphenyl)-4,4,5,5-teframetyl-l,3,2-dioxaborolane
The title compound (472 mg, 81%) was prepared from 4-bromo-2-chloro-l-phenoxybenzene (500 mg, 1.77 mmol) in a similar manner to that described for the preparation 2-phenoxy-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolane-2-yl)benzonitrile: Η NMR (DMSQ-d6, 400 MHz) δ 7.75 (s, IH), 7.60 (d, IH), 7.42 (t, 2H), 7.21 (t, IH), 7.02 (m, 3H), 1.30 (s, 12H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%>-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 15.53 min.

c) Trans-5 -(3 -chloro-4-phenoxyphenyl)-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo[2,3-^pyrimidm-4-__rnine
The title compound was prepared from 2-(3-chloro-4-phenoxyphenyl)-4,4,5,5-tetramethyl-l,3,2-dioxaborolane and tr «_.-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidine in a similar manner to that described for the preparation of trans-5- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-<i]pyrimidin-5-yl}-2-phenoxybenzonitrile: Η NMR (DMSO-d6, 400 MHz) δ 8.14 (s, IH), 7.79 (s, IH), 7.65 (s, IH), 7.38 (m, 3H), 7.16 (t, 2H), 7.04 (d, 2H), 6.24 (bs, 2H), 4.55 (m, IH), 2.2-2.6 (m, 9H), 2.16 (s, 3H), 1.89 (m, 6H), 1.48 (m, 2H); MS MET 517.3; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 15.75 min.

Example 381: r_.«-?-5-(3-fluoro-4-phenoxyphenyl)-7-[4-(4-methylpiperaz o)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-4-amine bisacetate a) 4-bromo-2-fluoro-l-phenoxybenzene
The title compound was prepared in a 33%> yield from 4-bromo-2-fluorophenol and phenyl boronic acid in a manner similar to that described for the preparation of 4-bromo-2-chloro-l-ρhenoxybenzene: XH NMR (DMSO-d6, 400 MHz) δ 7.75 (dd, IH), 7.40 (m, 3H), 7.15 (m, 2H), 7.01 (dd, 2H); MS MH+ 267.2; RP-HPLC
(Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammomum acetate over 10 min, 1 ml/min) t- 13.57 min.

b) 2-(3-fluoro-4-phenoxyphenyl)-4,4,5,5-tetrametyl-l,3,2-dioxaborolane
The title compound was prepared in a 65% from 4-bromo-2-fluoro-l-phenoxybenzene in a similar manner to that described for the preparation 2-phenoxy-5-(4,4,5,5-teframethyl- 1 ,3,2-dioxaborolane-2-yl)benzonitrile: λH NMR DMSO-dg, 400 MHz) δ 7.49 (dd, 2H), 7.41 (m, 2H), 7.18 (t, IH), 7.08 (d, IH), 7.05 (d, 2H), 1.30 (s, 12H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml min) t_ 14.63 min. c) trans-4- {4-chloro-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-5-yl}-2-fluorophenyl phenyl ether
The title compound was prepared in an 85%o yield from tr .._.-4-chloro-5-iodo-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -_ ]pyrimidine and 2-(3 -fluoro-4-phenoxyphenyl)-4,4,5,5-tetrametyl-l,3,2-dioxaborolane in a similar manner to that described for the preparation of tr-.«5-5-(4-chloro-7-(4-(4-methylpiperazino)cyclohexyl)-7H-pynolo[2,3-c?]pyrimidin-5-yl)-2-phenoxybenzonitrile: Η NMR (DMSO-d6, 400 MHz) δ 8.67 (s, IH), 8.07 (s, IH), 7.58 (d, IH), 7.41 (m, 3H), 7.22 (m, IH), 7.16 (m, IH), 7.04 (d, 2H), 4.70 (m, IH), 2.2-2.6 (m, 9H), 2.16 (s, 3H), 1.94-2.03 (m, 6H), 1.48 (m, 2H); MS MET 520.3; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 9.45 min.

d) rα«5-5-(3-fluoro-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-4-amine bisacetate
The title compound was prepared in a 75% yield from trα«5-4-{4-chloro-7- [4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_f]pyrimidin-5-yl}-2-fluorophenyl phenyl ether in a similar manner to that described for the preparation of tr_z«5-5-(3-methoxy-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyπolo[2,3- yrimidin-4-amine acetate: Η NMR (DMSO-d6, 400 MHz) δ 8.14 (s, IH), 7.52 (s, IH), 7.40 (m, 3H), 7.27 (m, 2H), 7.13 (t, IH), 7.06 (d, 2H), 6.25 (bs, 2H), 4.55 (m, IH), 2.2-2.6 (m, 9H), 2.14 (s, 3H), 1.8-2.0 (m, 6H), 1.92 (s, 6H), 1.44 (m, 2H); MS MH* 501; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml min) t. 7.52 min.

Example 382: Er_.«5-5-(3-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-c ]pyrimidin-4-amine
a) 4-bromo-2-methyl- 1 -phenoxybenzene
The title compound was prepared in a 35% yield from 4-bromo-2-methylphenol and phenyl boronic acid in a manner similar to that described for the preparation of 4-bromo-2-chloro-l-phenoxybenzene: Η NMR (DMSO-d6, 400 MHz) δ 7.54 (s, IH), 7.35 (m, 3H), 7.12 (t, IH), 6.93 (d, 2H), 6.82 (d, 2H), 2.18 (s, 3H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammonium acetate over 10 min, 1 ml min) t_ 14.25min.

b) 2-methyl-4-(4,4,5,5-teframetyl-l,3,2-dioxaborolan-2-yl)phenyl phenyl ether
The title compound was prepared in a 77%> from 4-bromo-2-methyl-l-phenoxybenzene in a similar manner to that described for the preparation 2-phenoxy-5-(4,4,5,5-teframexhyl-l,3,2-dioxaborolane-2-yl)benzonitrile: 1H NMR (DMSO- 5, 400 MHz) δ 7.67 (s, IH), 7.50 (d, IH), 7.38 (t, 2H), 7.14 (t, IH), 6.94 (d, 2H), 6.80 (d, IH), 2.21 (t, 3H); TLC (heptane/ethyl acetate 9:1) RfO.6.

c) tr «5-4-{4-chloro-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl}-2-methylphenyl phenyl ether
The title compound was prepared in an 82% yield from from traTw^-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-(i]pyrimidine and 2-methyl-4-(4,4,5,5-tetrametyl-l,3,2-dioxaborolan-2-yl)phenyl phenyl ether in similar manner to that described for the preparation of tr n_.-5-(4-chloro-7-(4-(4-methylpiperazino)cyclohexyl)-7H-pynolo[2,3-_ ]pyrimidin-5-yl)-2-phenoxybenzonitrile: Η NMR DMSO-d6, 400 MHz) δ 8.65 (s, IH), 7.98 (s, IH), 7.49 (s, IH), 7.37 (m, 3H), 7.10 (t, IH), 6.95 (m, 3H), 4.72 (m, IH), 2.2-2.6 (m, 9H), 2.23 (s, 3H), 2.17 (s, 3H), 2.01 (m, 6H), 1.48 (m, 2H); MS MH+ 516.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammonium acetate over 10 min, 1 mVmin) t. 10.53 min.

d) rr_.π_.-5-(3-methyl-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-4-amine
The title compound was prepared in a 75% yield from trα«_.-4-{4-chloro-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-5-yl}-2-methylphenyl phenyl ether in a similar manner to that described for the preparation oftra«_.-5-(3-methoxy-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρyπolo[2,3-_φyrimidin-4-amine acetate: Η NMR (DMSO-./fi, 400 MHz) δ 8.13 (s, IH), 7.42 (m, 4H), 7.37 (t, 2H), 7.27(m, IH), 7.10 (t, IH), 6.97 (m, 3H), 6.11 (bs, 2H), 4.55 (m, IH), 2.2-2.6 (m, 9H), 2.22 (s, 3H), 2.14 (s, 3H), 1.7-2.0 (m, 6H), 1.45 (m, 2H); MS MH+ 497.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 5%-100%) acetonitrile-0.05 M ammonium acetate over 25 min, 1 ml/min) t- 15.93 min.

Example 383: rrø/._.-7-[4-(4-methylpiperazino)cyclohexyl]- 5-(3-nitro-4-phenoxyphenyl)-7H-pynolo[2,3-<i]pyrimidin-4-amine acetate
a) 4-bromo-2-mtro-l-phenoxybenzene
The title compound was prepared in a quantitative yield from 5-bromo-2-fluoro- nitrobenzene and phenol in a manner similar to that described for the preparation of 5-bromo-phenoxybenzonitrile: 'H NMR (DMSO-^, 400 MHz) δ 8.31 9d, IH), 7.86 (dd, IH), 7.45 (t, 2H), 7.25 (t, IH), 7.13 (d, IH), 7.08 (d, 2H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t_ 12.70 min.

b) 2-nitro-4-(4,4,5,5-teframetyl-l,3,2-dioxaborolan-2-yl)phenyl phenyl ether
The title compound was prepared in a 56%> from 4-bromo-2-nifro-l-phenoxybenzene in a similar manner to that described for the preparation 2-phenoxy-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolane-2-yl)benzonitrile: ]H NMR (DMSO-_ 400 MHz) δ 8.19 (d, IH), 7.91 (d, IH), 7.46 (t, 2H), 7.27 (t, IH), 7.14 (d, 2H), 7.06 (d, IH), 1.31 (s, 12H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%- 100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t. 14.02 min.

c) trαπ_.-7-[4-(4-methylpiperazino)cyclohexyl]- 5-(3-nitro-4-phenoxyphenyl)-7H-pynolo[2,3-rf]pyrimidin-4-amine acetate
The title compound was prepared from tr_.n-f-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-(_ ]pyrimidine and 2-nifro-4-(4,4,5,5-teframetyl- 1 ,3 ,2-dioxaborolan-2-yl)phenyl phenyl ether in a similar manner to that described for the preparation of tr_.π_.-5-(4-chloro-7-(4-(4-methylpiperazino)cyclohexyl)-7H-pynolo[2,3-_ ]pyrimidin-5-yl)-2-phenoxybenzonitrile: 'Η NMR (DMSO-d6, 400 MHz) δ 8.15 (s, IH), 8.08 (d, IH), 7.74 (d, IH), 7.46 (s, IH), 7.42 (t, 2H), 7.23 (m, 2H), 7.12 (d, 2H), 6.36 (bs, 2H), 4.56 (m, IH), 2.2-2.6 (m, 9H), 2.16 (s, 3H), 1.8-2.0 (m, 6H), 1.92 (s, 3H), 1.45 (m, 2H); MS MH+ 528.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 5%-100%o acetonitrile-0.05 M ammonium acetate over 25 min, 1 ml/min) t. 14.88 min.

Example 384: rα«^-5-(3-amino-4-phenoxyphenyl)-7-[4-(4-methylρiperazino)cyclohexyl]-7H-pynolo[2,3-^pvrimidin-4-amine A mixture of (135 mg, 0.256 mmol) in ethanol (5 mL) and water (2 mL) was heated at 80°C then freated with sodium dithionite (270 mg, 1.54 mmol) in three portions over approximately 15 minutes. Heating was continued for 15 minutes then more sodium dithionite (135 mg, 0.75 mmol) was added and heating was continued for 10 minutes. The mixture was freated with 15 wt % sodium hydroxide in water (1 mL) and then cooled to ambient temperature after 10 minutes. Acidification with acetic acid and preparative RP-HPLC followed by lyophilization gave a residue which was dissolved in ethanol (20 mL) and acetic acid (1 mL) then hydrogenated for 1 hour at ambient temperature and atmospheric pressure in the presence of 10% Pd-C (30 mg). Filtration and purification by RP-HPLC gave tra«_^-5-(3-amino-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-_φyrimidin-4-amine (26 mg, 20%): Η NMR (DMSO-Jtf, 400 MHz) δ 8.13 (s, IH), 7.35 (m, 3H), 7.06 (t, IH), 6.97 (d, 2H), 6.89 (m, 2H), 6.60 (m, IH), 6.2 (bs, 2H), 5.08 (bs, 2H), 4.54 (m, IH), 2.2-2.6 (m, 9H), 2.15 (s, 3H), 1.8-2.1 (m, 6H), 1.45 (m, 2H); MS MH - 498.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%o-100%> acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml min) t,. 6.90 min

Example 385: rr n_?-5-(3-(dimethylamino)-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-4-amine

A mixture of rr «-f-5-(3-amino-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-c ]pyrimidin-4-amine (120 mg, 0.241 mmol), 37% aqueous formaldehyde (80 mg, 0.966 mmol) and acetic acid (45 mg, 0.724) in 1,2-dichloroethane (5 mL) was stined at ambient temperature for 30 minutes then sodium triacetoxyborohydride (154 mg, 0.72 mmol) was added. The mixture was stined at ambient temperature for an additional 18 hours then the mixture was concentrated under reduced pressure and the residue was purified by preparative RP-ΗPLC to give trα/._.-5-(3-(dimethylamino)-4-phenoxyphenyl)-7-[4- (4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_/]pyrinfrdm-4-amine (10 mg, 12%):Η NMR (DMSO-dg, 400 MHz) δ 8.13 (s, IH), 7.45 (s, IH), 7.34 (t, 2H), 7.05 (m, 2H), 6.9-7.0 (m, 4H), 6.16 (bs, 2H), 4.55 (m, 2H), 2.78 (s, 6H), 2.2-2.6 (m, 9H), 2.23 (s, 3H), 1.8-2.0 (m, 6H), 1.45 (m, 2H); MS MH+ 526.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 5%>-50%> acetonitrile-0.05 M ammonium acetate over 25 min, 1 ml/min) t. 25.6 min.

Example 386: rr n5- -(5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-
acetate

A mixture of trα«_.-5-(3-amino-4-phenoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyι±n_ιidin-4-amine (120 mg, 0.241 mmol) in pyridine (2 mL) and dichloromethane (2 mL) was cooled to 0°C then treatd with acetyl chloride (25 mg, 0.3 mmol). The mixture was warmed to ambient temperature for one hour then the solvents were removed under reduced pressure and the residue purified by preparative RP-ΗPLC to give trαπ_?-Nl-(5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_^pyrimidin-5-yl}-2-phenoxyphenyl)acetamide acetate (45 mg, 35%):Η ΝMR (DMSO-d6, 400 MHz) δ 9.67 (bs, IH), 8.11 (s, IH), 8.00 (s, IH), 7.40 (t, 3H), 7.17 (m, 2H), 7.05 (d, 2H), 6.95 (d, IH), 6.30 (bs, 2H), 4.55 (m, IH), 2.2-2.6 (m, 9H), 2.14 (s, 3H), 2.04 (s, 3H), 1.8-2.0 (m, 6H), 1.89 (s, 3H), 1.44 (m, 2H); MS MH+ 540.5; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 5%.-50%> acetonitrile-0.05 M ammonium acetate over 25 min, 1 ml/min) tr 20.37 min.

Example 387: _Tr ?.-?-5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyπolo[2,3-_i]pyrimidin-5-yl} -2-phenoxybenzaldehyde xrismaleate

a) 4-bromo-2-phenoxybenzaldehyde
The title compound was prepared in a 46%> yield from 5-bromo-2-fluoro-benzaldehyde and phenol in a manner similar to that described for the preparation of 5-bromo-phenoxybenzonitrile: Η NMR (DM$0-dό, 400 MHz) δ 10.32 (s, IH), 7.92 (s, IH), 7.81 (d, IH), 7.46 (t, 2H), 7.27 (t, IH), 7.18 (d, 2H), 6.90 (d, IH) RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M
ammonium acetate over 10 min, 1 ml/min) t,. 13.08 min.

b) 2-phenoxy-5-(4,4,5,5-teframethyl-l,3,2-dioxaborolan-2-yl)benzaldehyde
The title compound was prepared in a 62% from 4-bromo-2-phenoxybenzaldehyde in a similar manner to that described for the preparation 2-phenoxy-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolane-2-yl)benzonitrile: 'H NMR (DMSO-- 400 MHz) δ 10.43 (s, IH), 8.14 (s, IH), 7.89 (d, IH), 7.48 (t, 2H), 7.27 (t, IH), 7.20 (d, 2H), 6.88 (d, IH), 1.30 (s, 12H); RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%o-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t_ 14.02min.

c) trans -5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-<f]pyrimidin-4-amine hydrochloride
A mixture of tr_.«1y-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidine (750 mg, 1.63 mmol) and 1,4-dioxane (10 mL) and 30%) aqueous ammonium hydroxide (10 mL) was heated at 120°C in a sealed tube for 18 hours. The mixture is cooled and lyophilized to give trα7.5-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-4-amine hydrochloride (750 mg, quantitative): Η NMR (DMSO-d6, 400 MHz) δ 10.2 (bs, IH), 8.08 (s, IH), 7.53 (s, IH), 6.59 (bs, 2H), 4.50 (m, IH), 2.5-3.2 (m, 9H), 2.51 (s, 3H), 1.8-2.0 (m, 6H), 1.46 (m, 2H); MS MH1" 441.2; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 25%-100% acetonitrile-0.05 M ammonium acetate over 10 min, 1 ml/min) t,. 3.85 min.

d) rr 7.-.-5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl} -2-phenoxybenzaldehyde trismaleate
A mixture of tran_f-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H- 1
-274- pynolo[2,3-_ ]pyri_mdin-4-amine hydrochloride (750 mg, 1.63 mmol), 2-phenoxy-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)benzaldehyde (635 mg, 1.96 mmol), sodium carbonate monohydrate (490 mg, 3.92mmol) and
tefraMs(triphenylphosphine)palladium(0) (115 mg, 0.1 mmol) in ethylene glycol dimethyl ether (40 mL) and water (20 mL) was heated at 85°C for 18 hours. The mixture was cooled, concentrated under reduced pressure then purified by preparative RP-HPLC then lyophilized to give traτ._.-5-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrirnidin-5-yl}-2-phenoxybenzaldehyde bisacetate (636 mg, 68%). A portion of this compound (75 mg, 0.132 mmol) was dissolved in ethanol (2 mL) and the treated with maleic acid (61 mg, 0.526 mmol) in ethanol (1 mL). The precipitate was collected by filfration and dried under reduced pressure: Η NMR (DMSO-d6, 400 MHz) δ 10.41 (s, IH), 8.22 (s, IH), 7.88 (s, IH), 7.72 (d, IH), 7.60 (s, IH), 7.47 (t, 2H), 7.24 (m, 3H), 7.08 (d, IH), 6.62 (bs, 2H), 6.16 (s, 6H), 4.61 (m, IH), 2.5-3.4 ( , 9H), 2.69 (s, 3H), 1.8-2.1 (m, 6H), 1.56 (m, 2H); MS MET 511.4; RP-HPLC (Hypersil HS, 5μm, 100A, 4.6 x 250 mm; 5%-100% acetonitrile-0.05 M ammomum acetate over 25 min, 1 ml/min) t. 14.55 min.

Example 388: Trans 2-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]-l-hydroxycyclohexylacetamide

A mixture of trans 4-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-^pyrimidin-7-yl]-l-hydroxycyclohexylmethyI cyanide (0.300 g, 0.68 mmol) and potassium carbonate (0.377g, 2.73 mmol) in dimethylsulfoxide (3 ml) was stined rapidly and 30% hydrogen peroxide (0.5 mL) was added dropwise, keeping the temperature at 20° C. The mixture was stined for seven hours at ambient temperature. Water (20 mL) was added, and a precipitate formed the mixture was extracted with ethyl acetate (3 x 10 mL), dried over magnesium sulfate, and concentrated. The residue was purified by preparative RP-ΗPLC (Rainin C18, 8μm, 300 A, 25 cm; 30% isocratic for five minutes, then 30%-60% acetonitrile - 0. IM ammonium acetate over 30 min, 21 ml/min). The acetonitrile was removed in vacuo and the aqueous mixture was lyopholyzed to give trans 2-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3--^pyrimidin-7-yl]-l-hydroxycyclohexylacetamide as a white solid (0.055 g, 0.12 mmol):
Η NMR (DMSO-cE. 400MΗz) δ 8.14 (s, IH), 7.48 (d, 2H), 7.47 (s, IH), 7.42 (t, 2H), 7.17 (t, IH), 7.06-7.13 (m, 4H), 6.11 (b, 2H), 5.18 (s, IH) 4.61-4.67 (m, IH),

1.91-2.00 (m, 2H), 1.82-1.86 (m, 4H), 1.57-1.65 (m, 2H);
RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) 1 14.46 min.;
MS: M1" 458.

Example 389: Trans 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3- _ ]pyrimidin-7-yl]- 1 -(hydroxymethyl)- 1 -cyclohexanol

A trans/cis (4:1) mixture of 7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine (0.300 g, 0.42 mmol) in 1,2-dimethoxyethane (15 mL) was reacted with 2.5 M aqueous potassium hydroxide (15 mL). The mixture was stined at reflux for eighteen hours. The mixture was cooled to room temperature and water (25 mL) was added. The precipitate which formed was filtered, washing with water (20 mL). The precipitate was purified by preparative RP-ΗPLC (Rainin C18, 8μm, 300 A, 25 cm; 30% isocratic for five minutes, then 30%-60% acetonitrile - 0.1M ammonium acetate over 30 min, 21 ml/min). The acetonitrile was removed in vacuo and the aqueous mixture was lyopholyzed to give to give a trans/cis (4:1) mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-J]pyrimidin-7-yl]-l-(hydroxymethyl)-l-cyclohexanol. The isomers were separated by NP-MPLC (ISCO RediSep™ (10 g), 2% isocratic for five minutes, then 2-10%) methanol-dichloromethane over 25 minutes, 20 mL/min). The solvent was removed in vacuo to give trans 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrirnidin-7-yl]-l-(hydroxymethyl)-l-cyclohexanol as a white solid (0.026 g, 0.60 mmol):
Η NMR (DMSO-</6> 400MΗz) δ 8.13 (s, IH), 7.53 (s, IH), 7.47 (d, 2H), 7.41 (t, 2H), 7.16 (t, IH), 7.05-7.12 (m, 4H), 6.08 (b, IH), 4.56-4.66 (m, IH), 4.39 (t, IH), 4.25 (s, IH), 3.55 (d, 2H), 1.85-2.00 (m, 4H), 1.76-1.84 (m, 2H), 1.43-1.54 (m, 2H); RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammomum acetate over 20 min, lmL/min) R_ 14.45 min.;
MS: M+431.

Example 390: 7-[l-(lH-2-imidazolylmethyl)-4-piperidyl]-5-(4-phenoxyphenyl)-7H- pynolo[2,3-^]pyrimidin-4-amine

A mixture of 5-(4-phenoxyphenyl)-7-(4-piperidyl)-7H-pyπolo[2,3- _ |pyrimidin-4-amine (0.096 g, 0.249 mmol) in dichloroethane (5 mL) was treated with 2-imidazolcarboxaldehyde (0.026 g, 0.274 mmol) and glacial acetic acid (0.030 g, 0.498 mmol). The reaction mixture stined for one hour at 0°C under nitrogen atmosphere. Sodium triacetoxyborohydride (0.132 g, 0.623 mmol) was added to the reaction mixture and stined at 0°C for 20 minutes. The ice bath was then removed, and the reaction mixture stined for 18 hours at ambient temperature under a nifrogen atmosphere. Additional 2-imidazolcarboxaldehyde (0.096 g, 0.996 mmol) was added to the reaction mixture, and stined for an additional 24 hours. ' Acetonitrile (2 mL) was added to the reaction mixture, and then was allowed to stir for an additional 72 hours. Sodium bicarbonate (0.126 g, 1.49 mmol) in water (5 mL) was added to the reaction mixture and stined for 2 hours. The layers were partitioned, and the aqueous layer was extracted with dichloromethane (150 mL). The combined organic layers were washed with water and brine (250 mL each), dried over magnesium sulfate, filtered and evaporated under reduced pressure. The front-running impurity (by TLC analysis) was removed by recrystallization using ethyl acetate: heptane. The filfrate from the recrystallization was evaporated under reduced pressure, and purified using a Flashtube chromatography column using 20%> methanol in dichloromethane was the eluent. Some impure product was isolated and this was purified by preparative ΗPLC. 7-[l-(lH-2-imidazolylmethyl)-4-piperidyl]- 5-(4-phenoxyphenyl)-7H-p3 -olo[2,3-_^pyrimidin-4-amme was isolated and was dried on the lyophilizer. Dichloromethane (3 mL) and 1 N sodium hydroxide (1 mL) was added to the product. The layers were separated using an Empore 1

-277- extraction cartridge. The dichloromethane layer was collected and evaporated under reduced pressure to give 7-[l-(lH-2-imidazolylmethyl)-4-piperidyl]-5-(4- phenoxyphenyl)-7H-pynolo[2,3-^pyrimidin-4-amine as a free base (0.011 g, 10%). Η NMR (CDC13, 400 MHz) δ 8.31 (s, IH), 7.43-7.35 (m, 4H), 7.2-7.1 (m, IH), 7.09-7.00 (m, 7H), 5.18 (s, 2H), 4.75-4.69 (m, IH), 3.73 (s, 2H), 3.03-2.96 (m, 2H), 2.43-2.37 (m, 2H), 2.15-2.08 (m, 4H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 μm, 2.1 x 50 mm; 5%-95%> acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rj 5.363 min.

Example 391 : Cis-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pyrrolo[2,3~d]pyrimidin-5-yl}-2-fluorophenyl)-l,3-benzoxazol-2-amine

N2-(4-bromo-2-fluorophenyl)- 1 ,3-benzoxazol-2-amine
To a solution of 4-bromo-2-fluoroaniline (l.OOg, 5.26 mmol) in toluence

' (25 mL) was added 2-chlorobenzoxazole (0.66 mL, 5.19 mmol, 1.1 equiv). The purple solution was heated at reflux for 30 min. and then at 100°C for 17 hours. The resulting white suspension/purple solution was cooled to room temperature and the precipitate was filtered. The filter cake was washed with five 2-mL portion of heptane to afford N2-(4-bromo-2-fluorophenyl)-l,3-benzoxazol-2-amine (1.480 g, 92%) as a light purple powder. RP-HPLC (25 to 100% CH3CΝ in 0.1 N aqueous ammonium acetate over 10 minutes at 1 mL/min using a Hypersil HS C18, 250 x 4.6 mm column) tr = 12.87 min., 97%; and m/z 306.7 (MIT).

N2-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl]-l,3- benzoxazol-2-amine
To a solution of N2-(4-bromo-2-fluorophenyl)-l,3-benzoxazol-2-amine (1.480 g, 4.819 mmol) in dimethylformamide (15 mL) under nitrogen was added bis(pinacolato)diboron (1.468 g, 5.781 mmol, 1.2 equiv), potassium acetate (1.419 g, 14.45 mmol, 3.0 equiv), and [1,1'- bis(diphenylphosphino)fenocene]dichloropalladium (II) complexed with
dichloromethane (1:1) (0.119g, 0.146 mmol, 0.03 equiv). The violet solution was stined at 80 °C for 20 hours and then cooled to room temperature. The resulting dark brown mixture was concentrated in vacuo to give a dark brown liquid which was purified via flash chromatography on silica gel (eluting with 30% ethyl acetate/heptane) to afford 2.28 g of a yellow solid. This material was triturated with heptane and the solid was collected to afford N2-[2-fluoro-4-(4, 4, 5, 5-tetramethyl- l,3,2-dioxaborolan-2-yl)phenyl]-l,3-benzoxazol-2-amine (0.961 g, 56%) as a white powder. RP-HPLC (25 to 100%) CH3CN in 0.1 N aqueous ammonium acetate over 10 minutes at 1 mL/min using a Hypersil HS C18, 250 x 4.6 mm column) fr = 13.80 min., 88%; and m/z 354.9 (MIT).

Cis-N2-(4-{4-amino- 7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pyrrolo[2, 3- d]pyrimidin-5-yl}-2-fluorophynyl)-l, 3-benzoxazol-2-amine
To a solution of c._f-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-^pyrimidin-4-amine (0.100 g, 0.227 mmol) in ethylene glycol dimethyl ether (3 mL) and water (1.5 mL) under nifrogen was added N2-[2-fluoro-4-(4,4,5,5- teframethyl-l,3,2-dioxaborolan-2-yl)phenyl]-l,3-benzoxazol-2-amine (0.100 g, 0.283 mmol, 1.25 equiv.), tefra s(triρhenylphosphine) palladium (0) (0.013 mg, 0.011 mmol, 0.05 equiv), and sodium carbonate monohydrate (0.070 mg, 0.568 mmol, 2.5 equiv). The solution was stined at 80 °C for 21 hours. The resulting yellow mixture was concenfrated in vacuo to give a yellow oil. Purification by preparative ΗPLC (25 to 100 % CΗ3CΝ in 0.1 N aqueous ammonium acetate over 20 minutes at 21 mL/min. using a 8μ Hypersil HS C18, 250 x 21 mm column, tr = 6.4-8.5 min.) afforded cis-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pyrrolo[2, 3-d]pyrimidin-5-yl}-2-fluorophenyl)-l, 3-benzoxazol-2-amine as a cream solid (34 mg, 28%). RP-HPLC (25 to 100% CH3CN in 0.1 N aqueous ammonium acetate over 10 min at 1 mL/min using a Hypersil HS C18, 250 x 4.6 mm column) tr = 7.167 min., 98%; and m/z 540.8 (MET).

Example 392:
Nl-4-(4-amino-7-{4-[4-(l-methylpiperidyl)piperidyl]-7H-pynolo[2,3-d]pyrirnidin-5-yl])-2-fluorophenyl-2,3-dichloro-l-benzenesulfonamide
Using Nl -4-[4-amino- 7-(4-piperidyl)-7H-pyrrolo[2, 3-d]pyrimidin-5-yl]-2-fluorophenyl-2,3-dichloro-l -benzenesulfonamide (0.075 mmol scalej, the above methodology was followed to afford Nl-4-(4-amino-7-{4-[4-(l-methylpiperidyl)piperidyl]- 7H-pyrrolo[2, 3-d]pyrimidin-5-yl])-2-fluorophenyl-2, 3-dichloro-1 -benzenesulfonamide (32 mg), RP-HPLC RT=2.46 min and m/z 632.1.

Example 393: 7-{4-[2-(4-Methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrinήdm-4-amine

a) 2- {4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-- ]pyrimidin-7-yl]cyclohexyliden} acetic acid
A solution of diisopropylamine (1.40 g, 13.82 mmol) in tetrahydrofuran (60 mL) was added into a solution of 2.2 M n-BuLi (6.29 mL, 13.82 mmol) in tetrahydrofuran (20 mL) at -78°C. A solution of diethylphosphonoacetic acid (1.29 g, 6.59 mmol) in tefrahydrofuran (60 mL) was added at -78°C under the atmosphere of nitrogen. The mixture was kept at -78°C for 20 minutes and allowed to warm up to ambient temperature. The mixture was then transfened into a suspension of 4-[4-__mmo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-( lpyrimidin-7-yl]cyclohexanone (2.50 g, 6.28 mmol) in tefrahydrofuran (150 mL) at -78°C quickly. The resulting mixture was allowed to warm up to ambient temperature overnight and the solvent was removed under reduced pressure. The residue was taken in water and adjusted PΗ to be 3-4 with IM aqueous acetic acid followed by extraction with ethyl acetate three times. The combined organic exfracts were washed with saturated aqueous sodium chloride solution, dried over magnesium sulfate, filtered and evaporated under reduced pressure to give 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-<i]pyrimidin-7-yl]cyclohexyliden} acetic acid (1.96 g, 4.44 mmol) as a white solid: Η NMR (DMSO- 6>400MΗz) 11.98 (br, IH), 7.45 (d, 2H), 7.41 (s, IH), 7.40 (d, 2H), 7.16 (t, IH), 7.08 (m, 4H), 6.10 (br, 2H), 5.70 (s, IH), 4.80 (m, IH), 2.50-1.90 (m, 8H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100% acetonitrile - 0.05M ammonium acetate over 10 min, lmL/min) R^ 9.70 min. MS: MET 441.

b) 2- {4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]cyclohexyl} acetic acid
A mixture of 2- {4-[4-anήno-5-(4-phenoxyphenyl)-7H-ρynolo[2,3-_ ]pyrimidin- 7-yl]cyclohexyliden} acetic acid (0.60 g, 1.36 mmol), 10% palladium on activated carbon (0.18 g), IM aqueous sodium hydroxide (9 mL) and ethanol (60 mL) was hydrogenated under an atmosphere of hydrogen (60 psi) for 20 hours and filtered through a pad of celite. The filtrate was evaporated under reduced pressure to yield a white solid which was taken into water (20 mL) followed by addition of IM acetic acid (20 mL) to precipitate the product. The suspension was then filtered and the solid was lyophilized to yield 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl} acetic acid (0.51 g, 1.15 mmol) as a white solid: lH NMR (DMSO- 6,400MΗz) 11.99 (br, IH), 8.13 (s, IH), 7.60 (s, IH), 7.49 (d, 2H), 7.41 (t, 2H), 7.15 (t, IH), 7.09 (m, 4H), 6.09 (br, 2H), 4.60 (m, IH), 2.20-1.24 (m, 11H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100%
acetonitrile - 0.05M ammonium acetate over 10 min, lmL/min) R,.9.62 min. MS: MET 443.

c) 2-{4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]cyclohexyl} - 1 -ethanol
A mixture of 2-{4-[4-an_ no-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-7-yl]cyclohexyl} acetic acid (0.23 g, 0.51 mmol) and lithium aluminum hydride (0.30 g, 7.89 mmol) in tetrahydrofuran (25 mL) was stined at ambient temperature overnight. Water (3 mL) was added followed by 15% aqueous sodium hydroxide (3.5 mL) and water (10.5 mL). Tetrahydrofuran (25 mL) was added and the mixture was stined for 1 hour. The mixture was filtered through a pad of celite. The filtrate was concentrated and lyophilized to yield 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-rf]pyrimidin-7-yl]cyclohexyl}-l-ethanol (0.18 g, 0.42 mmol) as a white solid: Η NMR (DMSO- d_t 400MΗz) δ 8.13 (s, IH), 7.51 (s, IH), 7.48 (d, 2H), 7.42 (t, 2H), 7.16 (t, IH), 7.09 (m, 4H), 6.10 (br, 2H), 4.57 (m, IH), 4.36 (t, IH), 3.47 (q, 2H), 2.70-1.65 (m, 10H);RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100%) over 10 min then 100%>-25%> over 2 min acetonitrile - 0.05M ammonium acetate, lmL/min) 1^ 10.28 min. MS: MIT 429.

d) 2- {4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]cyclohexyl} acetaldehyde
Dimethylsulfoxide (0.040 g, 0.51 mmol) was added into a solution of oxalyl chloride (0.033 g, 0.26 mmol) in dichloromethane (3 mL) at -78°C. The mixture was allowed to warm up to -40 °C and stined for 15 minutes followed by cooling down to -78°C again. 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_f|pyrimidin-7-yl]cyclohexyl}-l -ethanol (0.10 g, 0.23 mmol) was added followed by triethylamine (0.12 mg, 1.165 mmol). The mixture was allowed to warm up to 0°C and saturated aqueous sodium bicarbonate solution (10 mL) was added to quench the reaction. The organic layer was separated from the aqueous layer and washed with 10% aqueous sodium hydorgensulfate solution (4 mL), saturated aqueous sodium bicarbonate solution (4 mL) and saturated aqueous sodium chloride (4 mL). The organic layer was dried over magnesium sulfate and the solvent was removed under reduced pressure. The residue was purified by flash chromatography on silica gel using methanol / dichloromethane (5:95) as a mobile phase to yield 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-7-yl]cyclohexyl} acetaldehyde (0.053 g, 0.12 mmol) as a white solid: Η NMR (CDC13, 400MΗz) δ 9.81 (s, IH), 8.33 (s, IH), 7.68 (m, IH), 7.43 (d, 2H), 7.38 (t, 2H), 7.15 (t, IH), 7.07 (m, 4H), 5.14 (br, 2H), 4.72 (m, IH), 2.63-1.72 (m, 1 IH); TLC (methanol / dichloromethane = 5:95) R_ 0.41. MS: MH+ 427.

e) 7-{4-[2-(4-Methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo [2,3 - J]pyrimidin-4-amine
A mixture of 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrirnidin-7-yl]cyclohexyl}acetaldehyde (0.025 g, 0.059 mmol), N-methylpiperazine (0.0083 g, 0.083 mmol) and acetic acid (0.0035 g, 0.059 mmol) in 1,2-dichloroethane (0.4 mL) was stined for 10 min at ambient temperature and sodium
triacetoxyborohydride (0.019 g, 0.089 mmol) was added. The mixture was stined at ambient temperature under an atmosphere of nitrogen for 6 hours and the solvent removed under reduced to yield a yellow oil. The compound was purified by preparative RP-ΗPLC to yield 7-{4-[2-(4-methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-_φyrimidin-4-amine (0.016 g, 0.031 mmol) as a white solid: Η NMR (CDC13, 400MΗz) δ 8.27 (s, IH), 7.45 (d, 2H), 7.37 (t, 2H), 7.16 (t, IH), 7.09 (m, 5H), 5.57 (br, 2H), 4.73 (m, IH), 2.33 (s, 3H), 2.49-1.65 (m, 19H); RP-HPLC (Hypersil C 18, 5μm, 1 OOA, 250x4.6mm; 25%- 100% acetonitrile -0.05M ammonium acetate over 10 min, lmL/min) Rj 8.15 min. MS: MH4" 511.

Example 394: 7-[4-(2-Morpholinoethyl]cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyπolo [2,3 -rf]pyrimidin-4-amine

A similar procedure to the preparation 7-{4-[2-(4-methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-
yielded 7-[4-(2-Morpholinoethyl]cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine (0.015 g, 0.030 mmol) as a white solid: Η NMR (CDC13, 400MΗz) δ 8.32 (br, IH), 7.44 (d, 2H), 7.38 (t, 2H), 7.15 (t, IH), 7.08 (m, 5H), 5.26 (br, 2H), 4.73 (m, IH), 3.74 (m, 4H), 2.80-1.64 (m, 15H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100% acetonitrile -0.05M ammonium acetate over 10 min, lmL/min) R_ 8.50 min. MS: MH4" 498.

Example 395: Nl -Methyl-2- {4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]cyclohexyl} acetamide

A mixture of 2-{4-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrin_ddin-7-yl]cyclohexyl} acetic acid (0.094 g, 0.21 mmol), l-hydroxy-7-azabenzotriazole (0.044 g, 0.32 mmol), l-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride (0.082 g, 0.43 mmol), 2M methylamine in tefrahydrofuran (2.13 mL, 4.26 mmol) and NN-dimethylformamide (1.50 mL) was heated at 60°C overnight. The mixture was allowed to cool to ambient temperature and purified by preparative RP-ΗPLC to yield Nl -methyl-2- {4-[4-amino-5-(4-phenoxyρhenyl)-7H-pynolo[2,3-^pyrimidin-7-yl]cyclohexyl} acetamide (0.025 g, 0.055mmol) as a white solid: !Η ΝMR (CDC13, 400MHz) δ 8.24 (s, IH), 7.42 (d, 2H), 7.38 (t, 2H), 7.16 (t, IH), 7.07 (m, 5H), 5.82 (br, 2H), 5.62 (br, IH), 4.70 (m, IH), 2.83 (d, 3H), 2.36-1.70 (m, 11H); MS: MH+456; TLC (ethyl acetate) Rf 0.34; RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%>-100%> acetonitrile - 0.05M ammonium acetate over 10 min, lmL/min) R_ 9.32 min.

Example 396: 7-{4-[2-(Dimethylamino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo [2,3 -_φyrimidin-4-amine

A similar procedure to the preparation 7-{4-[2-(4-methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrirmdin-4-_umine yielded 7- {4-[2-(dimemylamino)ethyl]cyclohexyl} -5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-4-amine (0.001 g, 0.002 mmol) as a white solid: Η ΝMR (CDC13, 400MΗz) δ 8.19 (s, IH), 7.63 (br, 2H), 7.44 (d, 2H), 7.38 (t, 2H), 7.17 (s, IH), 7.15 (t, IH), 7.07 (t, 4H), 4.72 (m, IH), 2.76 (m, 2H), 2.58 (s, 6H), 2.03-1.75 (m, 9H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100% acetonitrile - 0.05M ammomum acetate over 10 min, lmL/min) R. 8.17 min. MS: MH4" 456.

Example 397: ΛT-(2-{4-[4-Amino-5-(4-ρhenoxyρhenyl)-7H-ρynolo[2,3-_f]pyrimidin-7-yl]cyclohexyl} ethyl)-Nl N2JV2-trimethyl-l ,2-ethanediamine

A similar procedure to the preparation 7-{4-[2-(4-methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-c ]pyrimidin-4-amine yielded -(2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3--i]pyrimidin-7-yl]cyclohexyl}ethyl)-NlN2N2-trimethyl-l,2-ethanediamine (0.0015 g, 0.003 mmol) as a white solid: Η ΝMR (CDC13_ 400MΗz) δ 8.20 (s, IH), 8.11 (br, 2H), 7.43 (d, 2H), 7.38 (t, 2H), 7.15 (m, 2H), 7.06 (t, 4H), 4.70 (m, IH), 2.44 (s, 3H), 2.04 (s, 6H), 2.80-1.71 (m, 17H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100% acetonitrile - 0.05M ammonium acetate over 10 min, lmL/min) 1^ 8.70 min. MS: MH+ 513.

Example 398: Ethyl 3-[(2-{4-[4-amino-5-(4-ρhenoxyphenyl)-7H-ρynolo[2,3- ]pyrimidin-7-yl] cyclohexyl} ethyl)amino]propanoate

A similar procedure to the preparation 7-{4-[2-(4-methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine yielded ethyl 3-[(2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-</]pyrimidin-7-yl]cyclohexyl}ethyl)amino]propanoate (0.004 g, 0.008 mmol) as a white solid: Η ΝMR (CDC13> 400MΗz) δ 8.18 (s, IH), 7.58 (br, 2H), 7.39 (m, 4H), 7.08 (m, 6H), 4.72 (m, IH), 4.17 (q, 2H), 3.13 (m, 2H), 2.83 (m, 2H), 2.74 (m, 2H), 2.03-1.76 (m, 11H), 1.25 (t, 3H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%>-100%> acetonitrile - 0.05M ammonium acetate over 10 min, lmL/min) R, 8.98 min. MS: MH4" 528.

Example 399: 7ert-butyl 4-(2-{4-[4-amino-5-(4-phenoxyρhenyl)-7H-ρynolo[2,3-_f|pyrimidin-7-yl]cyclohexyl} ethyl)- 1 -piperazinecarboxylate

A similar procedure to the preparation 7- {4-[2-(4- methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-c ]pyrimidin-4-amine yielded tert-butyl 4-(2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo [2,3 -_ ]pyrimidin-7-yl] cyclohexyl} ethyl)- 1 -piperazinecarboxylate (0.002 g, 0.003 mmol) as a white solid: Η NMR (CDC13, 400MΗz) δ 8.27 (s, IH), 7.44 (d, 2H), 7.40 (t, 2H), 7.15 (t, IH), 7.08 (m, 5H), 5.62 (br, 2H), 4.73 (m, IH), 3.45 (m, 4H), 2.43-1.23 (m, 17H), 1.46 (s, 9H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100% over 10 min then 100%-25% over 2 min and stayed at 25% for 3 min acetonitrile - 0.05M ammonium acetate, lmL/min) R( 12.22 min. MS: MIT 597.

Example 400: 7-[4-(2-{[3-(lH-l-imidazolyl)propyl]amino}ethyl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine

A similar procedure to the preparation 7-{4-[2-(4-methylpiperazino)ethyl] cyclohexyl} -5 -(4-phenoxyphenyl)-7H-pynolo [2,3 - ]pyrimidin-4-amine yielded 7-[4-(2-{[3-(lH-l-imidazolyl)propyl]amino}ethyl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-φyrimidin-4-amine (0.003 g, 0.006 mmol) as a white solid: Η NMR (CDC13;

400MΗz) δ 8.23 (s, IH), 7.57 (s, IH), 7.43 (d, 2H), 7.38 (t, 2H), 7.16 (t, IH), 7.08 (m, 6H), 6.94 (br, IH), 5.98 (br, IH), 4.69 (m, IH), 4.58 (br, 2H), 4.07 (t, 2H),

2.69(m, 4H), 2.06-1.71 (m, 13H); RP-HPLC (Hypersil C18, 5μm, 100A,
250x4.6mm; 25%o-100%> acetonitrile - 0.05M ammomum acetate over 10 min, lmL/min) R_ 7.00 min. MS: MH4" 536.

Example 401: l-(2-{4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-ci]pyrimidin-7-yl] cyclohexyl} ethyl)-4-piperidinol

A similar procedure to the preparation 7-{4-[2-(4-methylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-rf]pyrimidin-4-amine yielded l-(2-{4-[4-amino-5-(4-phenoxyphenyl)-7H- pynolo[2,3-_ )pyrimidin-7-yl]cyclohexyl}ethyl)-4-piperidinol (0.007 g, 0.014 mmol) as a white solid: Η NMR (CDC13> 400MHz) δ 8.24 (s, IH), 7.44 (d, 2H), 7.38 (t, 2H), 7.15 (t, IH), 7.07 (m, 5H), 5.82 (br, 2H), 4.73 (m, IH), 3.85 (m, IH), 3.36 (br, IH), 2.94 (m, 2H), 2.53 (m, 4H), 2.05-1.75 (m, 15H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100%> acetonitrile - 0.05M ammonium acetate over 10 min, lmL/min) R_ 7.75 min. MS: MH4" 512.

Example 402 : 7- {4-[2-(4-Methyl- 1 ,4-diazepan- 1 -yl)ethyl]cyclohexyl} -5-(4-phenoxyphenyl)-7H-pyπolo[2,3-_φyrimidin-4-amine

A similar procedure to the preparation 7-{4-[2-(4-mexhylpiperazino)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrimidin-4-amine yielded 7-{4-[2-(4-methyl-l,4-diazepan-l-yl)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-4-anιine (0.003 g, 0.006 mmol) as a white solid: !Η NMR (CDC13> 400MHz) δ 8.26 (s, IH), 7.44 (d, 2H), 7.38 (t, 2H), 7.15 (t, IH), 7.08 (m, 5H), 5.71 (br, 2H), 4.71 (m, IH), 2.88 (m, 8H), 2.62 (t, 2H), 2.49(s, 3H), 1.98-1.70 (m, 13H); RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%o-100%> acetonitrile - 0.05M ammomum acetate over 10 min, lmL/min) R_ 7.90 min. MS: MH+ 525.

Example 403: 7-{4-[2-(lH-l-imidazolyl)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo [2,3-<i]pyrimidin-4-amine

a) 2- {4-[4-Amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-cT|pyrimidin-7-yl]cyclohexyl} ethyl methanesulfonate
Methanesulfonyl chloride (0.036 g, 0.31 mmol) was added into a solution of 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-__]pyrimidin-7-yl]cyclohexyl}-l-ethanol (0.11 g, 0.26 mmol) and triethylamine (2.5 mL) in dichloromethane (2.5 mL) at 0°C. The mixture was allowed to warm up to ambient temperature and stined overnight. Methanesulfonyl chloride (0.036 g, 0.31 mmol) and dichloromethane (2.5 mL) was added. The mixture was stined at ambient temperature for 17 hours.
Methanesulfonyl chloride (0.036 g, 0.31 mmol) and dichloromethane (2.5 mL) was added. The mixture was stined at ambient temperature for 4 hours. The solvent was removed under reduced pressure and the residue was purified by flash
chromatography on silica gel using methanol / dichloromethane (5:95) as a mobile phase to yield 2-{4-[4-anuno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-<i]pyrimidin-7-yl]cyclohexyl} ethyl methanesulfonate (0.10 g, 0.21 mmol) as a white solid: 'Η NMR (CDCl400MΗz) δ 8.31 (s, IH), 7.44 (d, 2H), 7.38 (t, 2H), 7.15 (t, IH), 7.08 (m, 5H), 5.31 (br, 2H), 4.73 (m, IH), 4.32 (t, 2H), 3.03 (s, 3H), 1.99-1.61 (m, 11H); TLC (methanol / dichloromethane = 5:95) R_ 0.37; RP-HPLC (Hypersil C18, 5μm, 100A, 250x4.6mm; 25%-100%> over 10 min then 100%-25% over 2 min acetonitrile -0.05M ammonium acetate, lmL/min) R_. 11.45 min; MS: MH+ 507.

b) 7- {4-[2-(lH-l -imidazolyl)ethyl]cyclohexyl}-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-4-amine
Imidazole (0.070 g, 1.03 mmol) was added into a solution of 2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrimidin-7-yl]cyclohexyl}ethyl
methanesulfonate (0.052 g, 0.10 mmol) in NN-dimethylformamide (2 mL). The mixture was stined at 40°C for 5 hours. 60% Sodium hydride in mineral oil (0.021 g, 052 mmol) was added and the mixture was stined at ambient temperature overnight. The solvent was removed under reduced pressure and the residue was purified by preparative RP-ΗPLC to yield 7-{4-[2-(lH-l-imidazolyl)ethyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-t ]pyrimidin-4-amine (0.030 g, 0.063 mmol) as a white solid: Η ΝMR (CDC13, 400MΗz) δ 8.30 (s, IH), 7.55 (br, IH), 7.44 (d, 2H), 7.42 (t, 2H), 7.16 (t, IH), 7.08 (m, 6H), 6.95 (br, IH), 5.49 (br, 2H), 4.72 (m, IH), 4.01 (t, 2H), 1.99-1.71 (m, 11H); RP-HPLC (Hypersil C18, 5μm, 100A,
250x4.6mm; 25%- 100% over 10 min acetonitrile - 0.05M ammonium acetate, lmL/min) R, 9.18 min; MS: MH+ 479.

Examples 404 and 405: Cis and tr_.«5-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-t ]pyrimidin-5-yl-2-methoxyphenyl)-3 -phenylpropanamide

To 4-[4-_unino-5-(4-amino-3-methoxyphenyl)-7H-pynolo[2,3-c?]pyrimidin-7-yl]-l-cyclohexanone (0.8 g, 2.3 mmol) in pyridme/dichloromethane (1:2.5, 45 ml) was added hydrocinnamylchloride (0.57 g, 3.4 mmol) in dichloromethane (2 ml) at 0°C under a flow of nitrogen. The solution was stined at 0°C for 2 hr. The solution was quenched with saturated aquoeus citric acid solution ( 50 ml) and the organic layer was washed with saturated aquoeus citric acid solution ( 2 x 50 ml). Dry, filter and concentrate to leave a brown foam (1.0 g). This was dissolved in dichloroethane (100 ml) and Ν-methylpiperazine (0.63 g, 6.3 mmol) and acetic acid (0.38 g, 6.3 mmol) was added. Sodium triacetoxyborohydride (0.67 g, 3.15 mmol) was added portionwise under nitrogen and the mixture stined overnight at room temperature. The mixture was quenched with saturated aq. ΝaΗCO3 solution (50 ml) and extracted with dichloromethane (3 x 100 ml). The combined organics were dried (sodium sulphate), filtered and evaporated to leave a sludge which was purified by flash silica gel column chromatography using dichloromethane / methanol (100/0 to 50/50 in 5% increments). The fractions conesponding to the less polar material were combined to give cis- Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ |pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide (0.26 g) as a glass. This was dissolved in ethylacetate (5 ml) and maleic acid (160 mg) in ethylacetate (2 ml) added. The resulting solid was filtered to give cis- Nl-(4-4-_unino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-«^pyrimidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide trimaleate salt (260 mg) as a white solid.

Analytical LC/MS conditions: Column: Pecosphere, C18, 3 um, 33x4.6 mm. Eluent:

0% B/A to 100%> B/A in 4.5 min.( B: acetonitrile, A: 50 mM ammonia acetate buffer, PΗ 4.5) , 3.5 mL/min. (rt = 2.86 mins, 568.4).

The fractions conesponding to the more polar material were combined to give frans-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3--f]pyrimidin- 5-yl-2-methoxyphenyl)-3-phenylpropanamide (0.11 g ) as a glass. This was dissolved in ethylacetate (5 ml) and freated with a solution of maleic acid (68 mg) in ethylacetate (2 ml). The resulting solid was filtered to give trans-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-(i]ρyrirnidin-5-yl-2-methoxyphenyl)-3-phenylpropanamide tri-maleate (94 mg) as a white solid.
Analytical LC/MS conditions: Column: Pecosphere, C18, 3 um, 33x4.6 mm. Eluent: 0% B/A to 100%) B/A in 4.5 min.( B: acetonitrile, A: 50 mM ammonia acetate buffer, PΗ 4.5) , 3.5 mL/min. (rt = 2.68mins, 568.2).

Examples 406 and 407: Cis and trans -5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-4-amine

4- [4-amino-5 -(4-amino-3 -methoxyphenyl)-7H-pynolo [2,3 -_f]pyrimidin-7-yl]-l-cyclohexanone (2.25 g, 6.5 mmol), acetic acid (1.17 g, 19.5 mmol) and Ν-methylpiperazine (1.95 g, 19.5 mmol) were dissolved in dichloroethane (200 ml). Sodium triacetoxyborohydride (2.07 g, 9.75 mmol) was added portionwise and the mixture stined at room temperature overnight. Saturated sodium bicarbonate solution (150 ml) was added and the aqueous layer exfracted with dichloromethane (3 x 100 ml). The combined organics were washed with water, dried (sodium sulphate), filtered and evaporated to leave a semi-solid whaich was purified by flash silica gel column chromatography using CΗ2C12 / methanol (0%> MeOH to 50%> MeOH in 5%> increments). The fractions conesponding to the faster running material were combined and evaporated to give cis- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-4-amine (1.2 g, 43%>) as a cream solid. Η ΝMR (d6-DMSO): δ 8.1 (1Η, s), 7.11 (1Η, s), 6.87 (1Η, s), 6.79 (1Η, d), 6.05 (2Η, bs), 4.80 (2H, bs), 4.64 (IH, m), 4.08 (IH, m), 3.82 (3H, s), 3.17 (2H, m), 2.37 (6H, m), 2.21 (3H, s), 2.08 (4H, m), 1.70 (2H, m), 1.53 (2H, m).
HPLC (rt= 11.24 min, 97.6%).
The fractions conesponding to the slower πmning material were combined and evaporated to give trans- 5-(4-amino-3-methoxyphenyl)-7-[4-(4- methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidm-4-amine (0.4 g, 14%) as a white solid. Η NMR (d6-DMSO): δ 8.10 (IH, s), 7.26 (IH, s), 6.87 (IH, s), 6.77 (IH, d), 6.71 (IH, d), 6.05 (2H, bs), 4.79 (2H, s), 4.52 (IH, m), 3.81 (3H, s), 3.35 (IH, m), 2.50 (5H, m), 2.31 (5H, m), 2.14 (IH, m), 1.97 (6H, m), 1.45 (2H, m). HPLC (rt = 10.13 min, 97.9%).

To a solution of trans- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazmo)cyclohexyl]-7H-pynolo[2,3-_^pyrimidin-4-amine (30 mg, 0.069 mmol) in pyridine (0.5 ml) was added the appropriate acid chloride (2 eq., 0.138 mmol). . The vials were capped and shaken overnight on an orbital shaker. LCMS (Micromass- Column: Pecosphere, C18, 3 um, 33x4.6 mm. Eluents: 0%o B/A to 100% B/A in 4.5 min.( B: acetonitrile, A: 50 mM ammonia acetate buffer, PΗ 4.5) , 3.5 mL/min.) ofthe resulting mixtures showed presence of product in all cases. The solutions were evaporated to dryness and the resulting residues were re- dissolved in DMF (1 ml) and purified by reverse phase prep. ΗPLC (Hypersil BSD C18, 5 um, 100x21mm, 0%>-100%> acetonitrile/0.05M ammomum acetate over 10 min, 25.0 mL/min). The resulting products were further purified by dissolving in
dichloromethane (4 ml) and washed with 1.0 N sodium hydroxide (2 ml) to give the conesponding products. The compounds are detailed below alongwith the appropriate Η NMR and LCMS data

Example 408: trans- Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-rf]pyrimidin-5-yl-2-methoxyphenyl)-2-phenylcyclopropane-l-carboxamide

Η ΝMR (d6-DMSO): δ 9.54 (1Η, s), 8.13 (1Η, s), 8.08 (2Η, d, J= 11.2 Hz), 7.44 (IH, s), 7.32 (2H, m), 7.20 (3H, m), 6.99 (IH, m), 6.11 (2H, bs), 4.55 (IH, s), 3.88 (3H, s), 2.50 (6H, m), 2.38 (6H, m), 2.14 (3H, s), 1.93 (6H, m), 1.47 (3H, m) LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.69 min. MH4" 580.4.

Example 409: trans - Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_^pyriir din-5-yl-2-methoxyphenyl)-4-dimethylanimobenzamide

Η ΝMR (CDC13): δ 8.61 (1Η, d, J= 8 Ηz), 8.48 (1Η, s), 8.33 (1Η, s), 7.83

(2Η, d, J = 8 Hz), 7.11 (IH, d, J= 8 Hz), 7.02 (2H, d, J= 8 Hz), 6.74 (2H, d, J= 8 Hz), 5.15 (2H, bs), 4.69 (IH, m), 3.98 (3H, s), 3.06 (6H, s), 2.43-2.65 (8H, m), 2.30 (3H, s), 2.22(2H, m), 2.08 (2H, m), 1.85 (3H, m), 1.60 (2H, m)
LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R< 2.59 min. MH+ 582.9.

Example 410: trans-Nl -(4-4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-4-trifluoromethoxybenzamide

Η ΝMR (d6-DMSO): δ 9.63 (1Η, s), 8.15 (1Η, s), 8.09 (2Η, d, J= 11.6 Hz), 7.85 (IH, d, J= 8 Hz), 7.52 (3H, m), 7.17 (IH, s), 7.06 (IH, d, J= 8 Hz), 6.15 (2H, bs), 4.56 (IH, m), 3.89 (3H, s), 2.50 (4, m), 2.35 (5H, m), 2.15 (3H, s), 1.92 (6H, m), 1.47 (2H, m).
LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R_ 2.86 min. MH4" 624.1

Example 411 : traπ5-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidm-5-yl-2-memoxyphenyl)-4-xrifluoromethylbenzamide

Η ΝMR (d6-DMSO): δ 9.76 (1Η, s), 8.15 (3Η, m), 7.91 (2H, d, J= 8.4 Hz),

7.86 (IH, d, J= 8.4 Hz), 7.51 (IH, s), 7.19 (IH, s), 7.08 (IH, d, J= 8Hz), 6.15 (2H, bs), 4.56 (IH, m), 3.91 (3H, s), 2.50 (4H, m), 2.35 (5H, m), 2.32 (3H, s), 1.94 (6H, m), 1.46 (2, m)
LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R( 2.69 min. MH+ 608.4 Example 412: ct_.-N-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-<i]pyrimidin-5-yl-2-methoxyphenyl)-N-benzylurea

To a solution of cis- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo[2,3-_tlpyrimidm-4-amine (50 mg, 0.116 mmol) in pyridine (1 ml) at room temperature was added benzylisocyanate (15.4 mg, 0.116 mmol). The resulting solution was shaken overnight at room temperature on an orbital shaker and then submitted directly to preparative ΗPLC. The resulting product was further purified by dissolving in dichloromethane (4 ml) and washed with 1.0 Ν sodium hydroxide (2 ml) to furnish, after evaporation ofthe solvent, cz_.-N-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-cf]pyrimidin-5-yl-2-methoxyρhenyl)-N-benzylurea as a white powder.
Η ΝMR (CDC13): δ 8.32 (1Η, s), 8.18 (1Η, d), 7.34 (4Η, m), 7.26 (IH, m), 7.08 2H, m), 6.95 (2H, m), 5.25 (IH, m), 5.10 (2H, bs), 4.82 (IH, m), 4.48 (2H, m), 3.85 (3H, s), 2.47 (3H, m), 2.29 (4H, m), 2.12 (4H, m), 1.82 (3H, m), 1.62 (2H, m), 1.31 (4H, m), 0.89 (2H, m).
LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M
ammonium acetate over 5 min, 3.0 mL/min): Rt 2.57 min. MH4" 569.3

Example 413: cis- Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-(E)-3-phenyl-2-propenamide

To a solution of cis- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-4-amine (50 mg, 0.116 mmol) in pyridine (1 ml) at room temperature was added cinnamoylchloride (39 mg, 0.232 mmol) and the solution shaken overnight at room temperature. The resulting solution was submitted directly to prep ΗPLC. The resulting product was further purified by dissolving in dichloromethane (4 ml) and washed with 1.0 Ν sodium hydroxide (2 ml) to furnish, after evaporation ofthe solvent, cis- Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-5-yl-2- methoxyphenyl)-(E)-3-phenyl-2-propenamide as an off-white powder (42 mg).

Η NMR (CDC13): δ 8.62 (IH, d, J= 8.4 Hz), 8.34 (IH, s), 7.99 (IH, s), 7.77 (IH, d, J= 8 Hz), 7.57 (2H, m), 7.40 (3H, m), 7.15 (2H, m), 7.04 (IH, s), 6.63 (IH, d, J= 15.2 Hz), 5.16 (2H, bs), 4.83 (IH, m), 3.99 (3H, s), 2.61 (6H, m), 2.29 (4H, m), 2.12 (4H, m), 1.86 (4H, m), 1.65 (2H, m)
LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonixrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R, 2.82 min. MH4" 566.3

Example 414: C-5-Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-5-yl-2-methoxyphenyl)-2-phenylacetamide

To a solution of cis- 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-4-amine (50 mg, 0.116 mmol) in pyridine (1 ml) at room temperature was added phenacetyl chloride (36 mg, 0.232 mmol). The resulting solution was shaken overnight and then submitted directly for prep. ΗPLC. The resulting product was further purified by dissolving in dichloromethane (4 ml) and washed with 1.0 Ν sodium hydroxide (2 ml) to furnish, after evaporation of the solvent, cis-Nl -(4-4-amino-7- [4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-2-phenylacetamide as a white powder (15 mg).
Η ΝMR (CDC13): δ 8.44 (1Η, d, J= 8.4 Ηz), 8.42 (1Η, s), 7.83 (1Η, s), 7.37 (4Η, m), 7.06 (2H, m), 6.93 (IH, s), 5.12 (2H, s), 4.80 (IH, m), 3.80 (3H, s), 2.61 (6H, m), 2.29 (4H, m), 2.13 (4H, m), 1.98 (2H, m), 1.83 (2H, m), 1.62 (2H, m).
LC/MS (Pecosphere C 18, 3 um, 33x4.6 mm; 0%- 100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rj 2.58 min. MH4" 554.3

All the following compounds were made by parallel synthesis according to the following procedure:
To cis - 5-(4-amino-3-methoxyphenyl)-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- pyrimidin-4-amine (30 mg, 0.069 mmol) in pyridine (0.5 mL) was added the appropriated acid chloride (0.138 mmol) slowly while vortexing. The reaction mixtures were shaken for 5-20 hr. The reactions were monitored by analytical HPLC. For the reactions that still had starting material left after 5 hours, another two equivalents of acid chloride were added while vortexing. The crude reaction mixtures were purified by reverse phase preparative LC MS (Hypersil BSD C18, 5 um, 100x21mm, 0%-100% acetonitrile/0.05M ammonium acetate over 10 min, 25.0 mL/min). The resulting products were further purified by dissolving in dichloromethane (4 ml) and washed with l.O N sodium hydroxide (2 ml) to give the conesponding products. Product yields are from 38%> to 88% and purities are from 94% to 100%.

Example 415: Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-2-methoxyacetamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100%) acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.32 min. MH+ 508.3.

Example 416: Cis -(4-4-aιnino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-cTIpyrimidin-5-yl-2-methoxyphenyl)-2,6-difluorobenzamide,

LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): Rt 2.61 min. MΗ+ 576.3.

Example 417: Cis Nl -(4-4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-2-methoxybenzamide,
LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): Rt 3.02 min. MΗ+ 570.3.

Example 418: Cis M-(4-4-amino-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo[2,3-_^pyriιmdin-5-yl-2-me1hoxyphenyl)-2,6-dimethoxybenzamide,

LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.61 min. MH4" 600.3.

Example 419: Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynoIo[2,3- ]pyrimidm-5-yl-2-methoxyphenyl)-3,4-dichlorobenzamide,

LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 3.26 min. MΗ4" 608.3.

Example 420: Cis Nl-(4-4-amino-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-3-methoxybenzamide

LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.74 min. MΗ+ 570.3.

Example 421: Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- pyrimidin-5-yl-2-methoxyphenyl)-4-fluorobenzamide,

LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rj 2.78 min. MΗ+ 558.34.

Example 422: Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_ ]pyrimidin-5-yl-2-methoxyphenyl)-4-chlorobenzamide,

LC MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 3.00 min. MΗ4" 574.3.

Example 423
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3- -fjpyrimidin-5-yl-2-methoxyphenyl)-4-methoxybenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): R, 2.76 min. MΗ4" 570.3.

Example 424
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-</pyriιrύdin-5-yl-2-methoxyphenyl)-4-(trifluoromethyl)berιzamide, LC/MS
(Pecosphere C18, 3 um, 33x4.6 mm; 0%-100%> acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 3.26 min. MH4" 608.3.

Example 425
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pyπolo[2,3-_f|pyrimidin-5-yl-2-methoxyphenyl)-2-phenoxyacetamide, LC/MS (Pecosphere C18,

3 um, 33x4.6 mm; 0%o-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): 1^ 2.94 min. MΗ4" 570.3.
Example 426
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_f]pyrimidin-5-yl-2-methoxyphenyl)-2-(4-chlorophenoxy)acetamide, LC/MS

(Pecosphere C18, 3 um, 33x4.6 mm; 0%o-100%> acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rj 3.13 min. MET 604.3.

Example 427
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-ρynolo[2,3-c?]pyrimidin-5-yl-2-methoxyphenyl-cis-2-phenylcyclopropane-l-carboxamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): _ 3.16 min. MET 580.3.

Example 428
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-5-yl-2-methoxyphenyl)-3-nifrobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100% acetonixrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.90 min. MΗ+ 585.3.

Example 429 Cis -(4-4-an_lno-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyi_imid_n-5-yl-2-methoxyphenyl)-2,5-difluorobenzamide, LC/MS (Pecosphere

C18, 3 um, 33x4.6 mm; 0%>-100%> acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.90 min. MΗ4" 576.3.

Example 430
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-ρynolo[2,3- _i]pyriι din-5-yl-2-methoxyphenyl)-2-(benzyloxy)acetamide, LC/MS (Pecosphere

C18, 3 um, 33x4.6 mm; 0%>-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.90 min. MΗ4" 584.3.
Example 431
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3- _i]pyrimidin-5-yl-2-methoxyphenyl)-3-cyanobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100%> acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R, 2.74 min. MΗ4" 565.6.

Example 432
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- _i]pyrimidin-5-yl-2-methoxyphenyl)-2,3-difluorobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): Rt 3.06 min. MET 576.3.

Example 433
Cis _v3-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- _i]pyrimidin-5-yl-2-methoxyphenyl)-6-chloronicotinamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100%) acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R, 2.53 min. MΗ+ 575.3.

Example 434
-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin- 5-yl-2-mexhoxyphenyl)-4-(tert-butoxy)benzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R, 3.32 min. MH4" 624.3.

Example 435
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3- ^pyrimidin-5-yl-2-methoxyphenyl)-2,4,6-frifluorobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.85 min. MΗ+ 594.4.
Example 436
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-c 3pyrimidin-5-yl-2-methoxyphenyl)-2-chloro-6-fluorobenzamide, LC/MS
(Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): R^ 2.76 min. MΗ4" 592.3.

Example 437
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- _ ]pyιrimidin-5-yl-2-methoxyphenyl)-4-(dimethylamino)benzamide, LC/MS
(Pecosphere C18, 3 um, 33x4.6 mm; 0%-100%> acetonitrile/0.05M ammomum acetate over 5 min, 3.0 mL/min): I^ 2.86 min. MET 583.3.

Example 438
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-c ]pyrimidin-5-yl-2-methoxyphenyl)-4-cyanobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.68 min. MΗ4" 565.3.

Example 439
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3- _i]pyrimidin-5-yl-2-methoxyphenyl)-4-nifrobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL min): Rt 2.84 min. MΗ4" 585.3.

Example 440
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-^pyrimidin-5-yl-2-methoxyphenyl)-3-fluorobenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): P^ 2.73 min. MΗ+ 558.1.

Example 441
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- <f]pyrimidin-5-yl-2-methoxyphenyl)-2,5-dimethoxybenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%.-100%ι acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.93 min. MΗ4" 600.2.

Example 442
Cis N5-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ]pyrimidin-5-yl-2-methoxyphenyl)-l,3-benzodioxole-5-carboxamide, LC/MS

(Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100%) acetonitrile/0.05M ammonium acetate over 5 min, 3.0 niL/min): Rt 2.73 min. MΗ+ 584.3.

Example 443
Cis Nl -(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3- ]pyrimidin-5-yl-2-methoxyphenyl)-2,6-dimethylbenzamide, LC/MS (Pecosphere

C18, 3 um, 33x4.6 mm; 0%o-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): P^ 2.84 min. MΗ4" 568.2.

Example 444
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_i]pyrimidin-5-yl-2-methoxyphenyl)-2-chloro-4-fluorobenzamide, LC MS
(Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): R_ 2.86 min. MΗ+ 592.1

Example 445
Cis N5-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- ^pyrimidin-5-yl-2-methoxyphenyl)-2, 1 ,3-benzoxadiazole-5-carboxamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%>-100%> acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.74 min. MH4" 582.1.

Example 446
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-ZH-pynolo[2,3- _i]pyrimidin-5-yl-2-methoxyphenyl)-4-phenylbutanamide, LC/MS (Pecosphere C18,

3 um, 33x4.6 mm; 0%>-100%) acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.92 min. MΗ4" 582.1.

Example 447
Cis N4-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- _i]pyrimidin-5-yl-2-methoxyphenyl)-l-methyl-5-propyl-lΗ-4-pyrazolecarboxamide,

LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%-100% acetonixrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.57 min. MH4" 586.4.

Example 448
Cis Nl-(4-4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-ci]pyrimidin-5-yl-2-methoxyphenyl)-2-methoxybenzamide, LC/MS (Pecosphere C18, 3 um, 33x4.6 mm; 0%ι-100%> acetonitrile/0.05M ammonium acetate over 5 min, 3.0 mL/min): Rt 2.32 min. MΗ+ 508.3.

Example 449
Cis-5-(4-phenoxyphenyl)-7- {4-[(5R)tefrahydro- lH-3-pynolylamino]cyclohexyl} — pynolo[2,3-rf]pyrimidin-4-amine
A mixture of 4-[4-annno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_f]pyrimidin-7-y rjcyclohexanone (l.OOg, 2.51 mmol), (3R)-(+)-3-aminopynolidine (0.65 g, 7.5 mmol), and acetic acid (0.43 mL, 7.5 mmol) in 1,2-dichloroethane (50 mL) was stined at ambient temperature under an atmosphere of nifrogen for 45 minutes. Sodium triacetoxyborohydride (0.691 g, 3.26 mmol) was added and the mixture stined at ambient temperature for 15 hours. Water (50 mL) and sodium bicarbonate (1.35 g, 16.1 mmol) were added and the mixture was stined for 45 minutes. The reaction mixture was transfened to a separatory funnel and the organic layer was separated. The organic portion was dried over magnesium sulfate, filtered, and the solvent removed under reduced pressure to yield an oily brown solid. The compound was purified by flash chromatography on silica gel (1 L 10% MeOH in CH2C12, then 1 L 20% MeOH in CH2C12, followed by 500 mL 30% MeOH in CH2C12) to yield cis-5-(4-phenoxyphenyl)-7-{4-[(5R)tetrahydro-lH-3-pynolylamino]cyclohexyl}-7H-pvnolo[2,3- ]pyrimidin-4-amine as a beige solid (0.285 g, 0.608 mmol): Η NMR (d6 DMSO, 400 MHz): δH 8.13 (IH, s), 7.39-7.49 (5H, m), 7.07-7.17 (5H, m), 6.09 (2H, bs), 4.65 (IH, m), 3.29-3.35 (3H, m), 2.63-2.67 (2H, m), 1.94-2.51 (8H, m), 1.46-1.80 (4H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%>-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rt 12.59 min.

The following compound was made in a similar manner to cis-5-(4-phenoxyphenyl)-7-{4-[(5R)tefrahydro-lH-3-pynolylaπιino]cyclohexyl}-7H-pynolo[2,3-_ ]pyrimidin-4-amine.

Example 450
Cis-5-(4-phenoxyphenyl)-7- {4-[(3 S)tetrahydro- lH-3-pynolylamino]cyclohexyl} -7H-pyπolo[2,3-_φyrimidin-4-amine: 1Η NMR (d6 DMSO, 400 MΗz): δH 8.13

(IH, s), 7.37-7.49 (5H, m), 7.07-7.17 (5H, m), 6.11 (2H, bs), 4.64 (IH, m), 3.17-3.33 (3H, m), 2.49-2.56 (2H, m), 1.90-2.36 (8H, m), 1.58-1.89 (4H, m); RP-HPLC (Delta Pak C18, 5μm, 300 A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rt 12.75 min.

Examples451 and 452
Cis-l-{4-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyriιnidin-7-yl] cyclohexyl} -4-piperidinol dimaleate salt and frans-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]cyclohexyl}-4-piperidinol dimaleate salt
A mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin- 7-yl]cyclohexanone (0.997 g, 2.50 mmol), 4-hydroxypiperidine (0.760 g, 7.5 mmol), sodium triacetoxyborohydride (0.689 g, 3.25 mmol), and acetic acid (0.43 mL, 7.5 mmol) in 1,2-dichloroethane (50 mL) was stined at 80 °C under an atmosphere of mfrogen for 58 hours. The reaction mixture was cooled and water (50 mL) and sodium bicarbonate (1.35 g, 16.1 mmol) were added and the mixture was stined for 2 hours. The organic layer was separated, dried over magnesium sulfate, filtered, and the solvent removed under reduced pressure to yield a brown solid. The cis and trans isomers were purified by flash chromatography on silica gel (1 L 5%> MeOH in CH2C12, then 500 mL 10% MeOH in CH2C12, 500 mL 50:45:5 CH2Cl2:MeOH:Et3N, and 500 mL 45:45:10 CH2Cl2:MeOH:Et3N). In order to remove residual Et3N, each ofthe products was taken up in CH2Cl washed with 40 mL water, the organic portion separated, dried over magnesium sulfate, filtered, and concentrated to yield cis-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-c ]pyrimidin-7-yl]cyclohexyl}-4-piperidinol as a brown solid (0.185 g, 0.382 mmol) and trans- 1- {4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-rf]pyrimidin-7-yl]cyclohexyl} -4-piperidinol as a brown solid (0.115 g, 0.237 mmol). The cis-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-7-yl]cyclohexyl} -4-piperidinol (0.185 g, 0.383 mmol) was dissolved in warm ethyl acetate (5 mL) then added to a solution of maleic acid (0.089 g, 0.765 mmol) in ethanol (5 mL). The mixture was cooled to ambient temperature and the solid was collected by filtration and dried to give cis-1 -{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-c ]pyrimidin-7-yl]cyclohexyl}-4-piperidinol dimaleate salt (0.202 g) as a yellow solid: Η NMR (d6 DMSO, 400 MHz): δH 8.61 (IH, bs), 8.25 (IH, s), 7.40-7.56 (5H, m), 7.08-7.19 (5H, m), 6.40 (2H, bs), 6.13 (4H, s), 5.00-5.20 (IH, m), 4.80 (IH, m), 3.03-3.95 (9H, m), 2.30-2.32 (2H, m), 1.63-1.99 (6H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) _ 13.33 min. MS: MH4" 484.

Trans-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3--i]pyrimidin-7-yl]cyclohexyl} -4-piperidinol dimaleate salt was prepared from the free base in the same manner: Η NMR (d6 DMSO, 400 MHz): δH 8.90 (IH, bs), 8.20 (IH, s), 7.40-7.48 (5H, m), 7.08-7.19 (5H, m), 6.45 (2H, bs), 6.14 (4H, s), 5.00-5.15 (IH, m), 4.75 (IH, m), 4.00 (IH, m), 3.05-3.90 (8H, m), 1.50-2.17 (8H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile- 0.1M ammonium acetate over 20 min, lmL/min) Rt 13.49 min. MS : MH4" 484.

Examples 453 and 454
Cis-7-{4-[(3R)-3-(dimethylamino)tetrahydro-lH-l-pynolyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine trimaleate salt and trans-7-{4-[(3R)-3-(dimethylamino)tefrahydro- IH- 1 -pyπolyl]cyclohexyl} -5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-4-amine trimaleate salt
A mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]cyclohexanone (l.OOg, 2.51 mmol), (3R)-(+)-3-(dimethylamino)pynolidine (0.86 g, 7.5 mmol), and acetic acid (0.43 mL, 7.5 mmol) in 1,2-dichloroethane (45 mL) was stined at ambient temperature under an atmosphere of nitrogen for 30 minutes. Sodium triacetoxyborohydride (0.689 g, 3.26 mmol) was added and the mixture stined at ambient temperature for 22 hours. Water (50 mL) and sodium bicarbonate (1.35 g, 16.1 mmol) were added and the mixture was stined for 3 hours. The reaction mixture was transfened to a separatory funnel and the organic layer was separated. The aqueous layer was exfracted with CΗ2C12 (20 mL), and the combined organic exfracts were dried over magnesium sulfate, filtered, and the solvent removed under reduced pressure to yield a red oil. The cis and trans isomers were purified by flash chromatography on silica gel (1 L 5%> MeOH in CH2C12, then 1 L 10% MeOH in CH2C12, 1 L 20%MeOH in CH2C12, 1 L 30% MeOH in CH2C12, 1 L 40% MeOH in CH2C12, and 1 L 50% MeOH in CH2C12) to yield cis-7- {4-[(3R)-3-(dimethylamino)tefrahydro- IH- 1 -pyπolyl]cyclohexyl} -5-(4-phenoxyphenyl)-7H-
as a pink solid (0.558 g, 1.12 mmol) and trans-7-{4-[(3R)-3-(dimethylamino)tetrahydro-lH-l-pynolyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine as a pink solid (0.210 g, 0.422 mmol). The cis-7- {4-[(3R)-3-(dimethylamino)tetrahydro-lH-l-pynolyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_^pyrimidin-4-amine (0.330 g, 0.670 mmol) was dissolved in warm ethanol (5 mL) then maleic acid (0.233 g, 2.01 mmol) in ethanol (5 mL) was added. The mixture was cooled to ambient temperature and the solid was collected by filtration and dried to give cis-7-{4-[(3R)-3-(dimethylamino)tetrahydro-lH-l-pynolyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_φyrimidin-4-amine trimaleate salt (0.622 g) as a beige solid: Η NMR (d6 DMSO, 400 MHz): δH 8.20 (IH, s), 7.41-7.48 (4H, m), 7.35 (lH,s), 7.08-7.19 (5H, m), 6.45 (2H, bs), 6.16 (6H, s), 4.70 (IH, m), 4.30 (2H, bs), 3.80 (IH, bs), 3.08 (3H, m), 2.76 (6H, s), 2.00-2.21 (6H, m), 1.74-1.72 (4H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rt 13.94 min. MS: MH4" 497.
Trans-7- {4- [(3R)-3 -(dimethylamino)tefrahydro- IH- 1 -pynolyl] cyclohexyl} -5-(4-phenoxyphenyl)-7H-pynolo[2,3-__]pyrimidin-4-amine trimaleate salt was prepared from the free base in the same manner: XΗ NMR (d6 DMSO, 400 MHz): δH 8.18 (IH, s), 7.40-7.47 (5H, m), 7.08-7.19 (5H, m), 6.33 (2H, bs), 6.12 (6H, s), 4.61 (IH, m), 3.07-3.60 (6H, m), 2.64 (6H, s), 2.18-2.20 (3H, m), 1.98-2.03 (5H, m), 1.55-1.58 (2H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) _ 14.08 min. MS: MH+ 497.

The following compounds were made in a similar manner to cis- and trans-7- {4-[(3R)-3-(dimethylamino)tefrahydro- IH- 1 -pyπolyl]cyclohexyl} -5-(4-phenoxyphenyl)-7H-pynolo[2,3-c ]pyrimidin-4-amine trimaleate salt.

Examples 455 and 456
Cis-7-{4-[(3S)-3-(dimethylamino)tefrahydro-lH-l-pynolyl]cyclohexyl}-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine trimaleate salt: H NMR (d6 DMSO, 400 MHz): δH 8.20 (IH, s), 7.42-7.48 (4H, m), 7.41 (lH,s), 7.08-7.17 (5H, m), 6.45 (2H, bs), 6.15 (6H, s), 4.67 (IH, m), 3.08-3.79 (6H, m), 2.76 (6H, s), 1.91-2.23 (6H, m), 1.74-1.77 (4H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lniL/min) Rj 13.89 min. MS: MH+ 497.

Trans-7-{4-[(3 -3-(dimethyIamino)tetrahydro-l_H-l-pyrroIyl]cyclohexyl}-5-(4- phenoxyphenyl)-7_fi -pyrrolo[2,3-<_ pyrimidin-4-amine trimaleate salt: lH NMR

(d6 DMSO, 400 MHz): δH 8.18 (IH, s), 7.40-748 (5H, m), 7.08-7.19 (5H, m), 6.34 (2H, bs), 6.12 (6H, s), 4.62 (IH, m), 3.24-3.91 (6H, m), 2.66 (6H, s), 2.18-2.21 (3H, m), 1.94-2.03 (5H, m), 1.55-1.58 (2H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%>-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) ^ 14.07 min. MS: MH 97.

Example 457
Cis-(3R)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7_ -pyrrolo[2,3-( |pyrimidin-7- yl] cyclohexyl} tetrahydro-lH-3-pyrrolol dimaleate salt
A mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrimidin- 7-yl]cyclohexanone (1.00 g, 2.51 mmol) and (R)-(+)-3-pynolidinol (0.62 mL, 7.5 ' mmol) in 1,2-dichloroethane (50 mL) was stined at ambient temperature under an atmosphere of nitrogen for 1.5 hours. Sodium triacetoxyborohydride (0.691 g, 3.26 mmol) was added and the mixture stined at ambient temperature for 22 hours. Water (50 mL) and sodium bicarbonate (1.34 g, 16.1 mmol) were added and the mixture was stined for 2 hours. The reaction mixture was transfened to a separatory funnel and the organic layer was separated, dried over magnesium sulfate, filtered, and the solvent removed under reduced pressure to yield a yellow-brown solid. The cis isomer was purified by flash chromatography on silica gel (1 L 5% MeOΗ in CΗ2C12, then 2 L 10% MeOH in CH2C12, 1 L 20% MeOH in CH2C12, and 1 L 40% MeOH in CH2C12) to yield cis-(3R)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H- pynolo[2,3-_i]pyrimidin-7-yl]cyclohexyl}tefrahydro-lH-3-pynolol as a pale yellow oil (0.529 g, 1.12 mmol). The cis-(3R)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H- pynolo[2,3-φyrimidin-7-yl]cyclohexyl}tefrahydro-lH-3-pynolol (0.464 g, 0.988 mmol) was dissolved in warm ethanol (5 mL) and added to a solution of maleic acid (0.344 g, 2.96 mmol) in ethanol (5 mL). The mixture was cooled to ambient temperature then the solid was collected by filfration and dried to give cis-(3R)-l-{4-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-7-yl]cyclohexyl}tefrahydro-lH-3-ρyπolol dimaleate salt (0.549 g) as a pale yellow solid: Η NMR (d6 DMSO, 400 MHz): δH 8.22 (IH, s), 7.41-7.50 (5H, m), 7.08-7.19 (5H, m), 6.49 (2H, bs), 6.13 (4H, s), 4.85 (IH, bs), 4.49 (IH, bs), 3.37-3.68 (5H, m), 1.92-2.18 (11H, m); HPLC: (Hypersil HS C18, 5μm, 254 nm, 250 x 4.6 mm; 25-100%> acetonitrile-O.lN ammonium acetate over 10 min, 1 m rnin) X_=1.161 min.

Examples 458
Trans-((2S)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-fiπpyrimidin-7-yl] cyclohexyl} tetrahydro-l_fiT-2-pyrroIyI)methanol dimaleate salt
A mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-(_7]pyrimidin-7-yl]cyclohexanone (0.200 g, 0.502 mmol) and (S)-(+)-2-ρyπolidinemethanol (0.152 g, 1.51 mmol) in ethanol (2 mL) was stined at ambient temperature under an atmosphere of nitrogen for 20 hours. The reaction mixture was filtered and concenfrated to afford a yellow oil. The crude product was combined with sodium borohydride (0.019 g, 0.502 mmol) in methanol (10 mL) and stined at ambient temperature under an atmosphere of nifrogen for 2 hours. Water (10 mL) was added, the organic portion was separated, and the aqueous portion was extracted with ethyl acetate (25 mL). The organic portions were combined, dried over magnesium sulfate, filtered, and concentrated to afford an oily yellow solid. Purification by flash chromatography on silica gel afforded trans-((2S)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-7-yl]cyclohexyl}tetrahydrq-lH-2-pynolyl)methanol (0.064 g, 0.132 mmol) as a white solid. The frans-((2S)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7/H-pynolo[2,3- ']pyrirnidin-7-yl]cyclohexyl}tetrahydro-lH-2-pynolyl)methanol (0.064 g, 0.132 mmol) was dissolved in warm ethyl acetate (2 mL) and a solution of maleic acid (0.031 g, 0.265 mmol) in ethanol (2 mL) was added. The mixture was cooled to ambient temperature and the solid was collected by filfration and dried to give frans-((2S)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ lpyrimidin-7-yl]cyclohexyl}tetrahydro-lH-2-pynolyl)methanol dimaleate salt (0.062 g) as a pale yellow solid: Η NMR (d6 DMSO, 400 MHz): δH 8.18 (IH, s), 7.40-7.47 (5H, m), 7.08-7.19 (5H, m), 6.32 (2H, bs), 6.13 (4H, s), 5.50 (IH, s), 4.65 (IH, m), 3.34-3.81 (6H, m), 1.77-2.09 (12H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rt 13.78 min. MS: MET 484.

The following compound was made in a similar manner to frans-((2S)- 1 - {4- [4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_f]pyrimidin-7-yl]cyclohexyl}tetrahydro-lH-2-pynolyl)methanol dimaleate salt:

Examples 459
Trans-((2R)-l-{4-[4-amino-5-(4-phenoxyphenyl)-7_ff-pyrrolo[2,3-«ηpyrimidin-7-yl] cyclohexyl} tetrahydro-l_l_T-2-pyrrolyl)methanol dimaleate salt: TΗ NMR (d6

DMSO, 400 MHz): δH 8.18 (IH, s), 7.40-7.47 (5H, m), 7.08-7.19 (5H, m), 6.31 (2H, bs), 6.13 (4H, s), 5.50 (IH, s), 4.65 (IH, m), 3.33-3.81 (6H, m), 1.78-2.06 (12H, m); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile -0.1M ammonium acetate over 20 min, lmL/min) R_ 13.83 min. MS: MH4" 484.

Cs-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7_H-pyrrolo[2,3-rf]pyrimidm-4-amme (Intermediate 1 )
In a heat dried flask, trimethylsulfoxonium iodide (1.33 g, 6 mmol) in dimethyl-sulfoxide (10 mL) was reacted with 60%> sodium hydride dispersion in mineral oil (0.22 g, 5.5 mmol). The mixture was stined at room temperature for 30 minutes and then cooled to 10°C. A solution of 4-[4-amino-5-(4-ρhenoxyphenyl)-7H-pynolo[2,3-__]pyrimidin-7-yl]-l-cyclohexanone (2 g, 5 mmol) in dimethylsulfoxide (10 mL) was added, and the mixture was stined at ambient temperature for 2 hours. Water (10 mL) was added and the mixture was extracted with ethyl acetate (2 x 20 mL). The organic phase was separated and dried over magnesium sulfate. The solvent was removed in vacuo to give ew-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-4-amine as a white solid (1.98 g, 4.8 mmol).
Η NMR (DMSO- _ 6)400MΗz) 8.15 (s, IH), 7.49 (d, 2H), 7.48 (s, IH), 7.42 (t, 2H), 7.16 (t, IH), 7.09 (d, 2H), 6.11 (bs, 2H), 4.72-4.78 (m, IH), 2.70 (s, 2H), 2.47-2.52 (m, 2H), 2.03-2.26 (m, 4H), 1.26-1.35 (m, 2H);
MS: M+413

7V «s-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7__7-pyrrolo[2,3-_ Jpyrimidin-4-amine (Intermediate 2)
To a mixture of 4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]-1-cyclohexanone (2 g, 5 mmol) in tefrahydrofuran (17.5 mL) were added trimethyl-sulfonium iodide (1.53 g, 7.5 mmol) in dimethyl sulfoxide (25 mL) and potassium-t-butoxide (7.5 mL, 7.5 mmol, 1 M in tefrahydrofuran) under an atmosphere of nifrogen at -5 °C. The mixture was stined at -5 °C for 1 hour. The mixture was poured into ice water (50 mL). The water phase was extracted with ethyl acetate (3 x 30 mL). The combined organic exfracts were washed with saturated ammonium chloride solution (2 x 30 mL), water (1 x 30 mL), and brine (2 x 30 mL) and dried over sodium sulfate. The solvent was removed under reduced pressure to yield 7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-4-amine (1.9 g, 4.6 mmol, 2:1 -mixture of trans and cis). The mixture was suspended in dichloromethane (3 mL). The solid was filtered and was washed with
dichloromethane (lmL) to yield 7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-_^pyrimidin-4-amine (0.994 g, 2.4 mmol, 4:1- mixture of trans and cis).
Η NMR for trans isomer (DMSO-d6, 400 MHz) δ 8.15 (s, IH), 7.45 (m, 5H), 7.16

(t, IH), 7.09(m, 4H), 6.11(br, 2H), 4.73 (br, IH), 2.65 (s, 2H), 2.16 (br, 6H), 1.38

(br, 2H)
RP-HPLC (Hypersil C18, 5/_m, 250 x 4.6 mm; 25% - 100% over 23 min with 0.1 M ammonium acetate, lmL/min) R_ 10.53 min.
MS: MH+ 413

Example 460
Cts-4-[4-amino-5-(4-phenoxyphenyl)-7___r-pyrrolo[2,3-rf]pyrimidin-7-yl]-l- hydroxycyclohexylmethyl cyanide
A mixture of ct?-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3- c?]pyrimidin-4-amine (0.750 g, 1.8 mmol), lithium perchlorate (0.290 g, 2.7 mmol) and potassium cyanide (0.177 g, 2.7 mmol) in acetonitrile (100 mL) was heated at 80°C for three days. Cooled to ambient temperature, diluted with water (50 mL) and extracted with diethyl ether (3x 50 mL). The combined organic phases were dried over magnesium sulfate. The solvent was removed in vacuo and the crude product was purified by flash column chromatography on silica using
dichloromethane/methanol (95:5). The solvent was removed in vacuo to give cis-4- [4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-Jpyrimidin-7-yl]-l- hydroxycyclohexylmethyl cyanide as a white solid (0.380 g, 0.9 mmol, 50%> yield): Η NMR (DMSO- ;,400MΗz) δ 8.14 (s, IH), 7.49 (d, 2H), 7.42 (t, 2H), 7.37 (s, ' IH), 7.16 (t, IH), 7.09 (d, 2H), 6.11 (bs, 2H), 4.51-4.62 (m, IH), 2.66 (s, 2H), 2.10- 2.29 (m, 2H), 1.72-1.87 (m, 4H), 1.60-1.72 (m, 2H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rt 15.90 min.;
MS: M4" 440

Example 461
r_*α«s-4-[4-amino-5-(4-phenoxyphenyl)-7_?_r-pyrroIo[2,3-rf]pyrimidin-7-yl]-l- hydroxycyclohexylmethyl cyanide
Ring opening of tra«1s,-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H- pynolo[2,3-< Ipyrimidin-4-amine (1 g, 2.4 mmol) with cyanide as described above led to tra n5-4-[4-aπύno-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_^pyr_midin-7-yl]-l - hydroxycyclohexyl-methyl cyanide as a white solid ( 0.435 g, 1.03 mmol, 43%> yield):

Η NMR (DMSO-_ 6,400MHz) δ 8.14 (s, IH), 7.63 (s, IH), 7.47 (d, 2H), 7.43 (t, 2H), 7.15-7.07 (m, 5H), 6.20 (br, 2H), 4.65 (m, IH), 2.98 (s, 2H), 1.92-1.86 (m, 6H), 1.80-1.65 (m, 2H),
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, 1 mL/min) Rt 15.88.
MS: M+440

Example 462
Cis-1 -(2-aminoethyl)-4- [4-amino-5-(4-phenoxyphenyl)-7ET-py rrolo [2,3-rflpyrimidin-7-yI]-l-cyclohexanol
To cw-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]-l-hydroxy-cyclohexylmethyl cyanide (0.340 g, 0.77 mmol) in methanol (30 ml) and ammonium hydroxide (2 mL) was added Raney nickel (0.5 mL). The mixture was stined 18 hours under hydrogen (1 atm). The reaction mixture was filtered through celite and the solvent was removed in vacuo to give cw-l-(2-aminoethyl)-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-c ]pyrimidin-7-yl]- 1 -cyclohexanol as a white solid (0.230 g, 0.52 mmol):
'Η NMR (DMSO-rf6;400MΗz) δ 8.13 (s, IH), 7.48 (d, 2H), 7.42 (t, 2H), 7.36 (s, IH), 7.16 (t, IH), 7.09 (d, 2H), 6.09 (bs, 2H), 4.50-4.63 (m, IH), 2.77-2.87 (m, 2H), 2.09-2.24(m, 2H), 166-1.78 (m, 4H), 1.42-1.62 (m, 2H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rj 13.07 min.;
MS: M+444

Example 463
Jr w_.-l-(2-aminoethyl)-4-[4-amino-5-(4-phenoxyphenyl)-7_HT-pyrrolo[2,3-</]pyrimidin-7-yl]-l-cyclohexanol
Reduction of trfl«_.-4-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]-l-hydroxy-cyclohexylmethyl cyanide (0.448 g, 1.0 mmol) as described above for the cis -isomer yielded trans-1 -(2-aminoethyl)-4-[4-amino-5-(4-phenoxyphenyl)- 7H-pyπolo[2,3-^ρyrimidin-7-yl]-l-cyclohexanol (0.015 g, 0.034 mmol)
Η NMR (Chloroform-./, 400 MHz) δ 8.32 (s, IH), 7.44 (m, 4H), 7.15 (t, IH), 7.09 (m, 4H), 6.99 (s, IH), 5.13 (br, 2H), 4.77 (br, IH), 3.09 (br, 2H), 2.10 (br, 2H), 2.00 (br, 2H), 1.84 (br, 6H).
RP-HPLC (Hypersil C18, 5μm, 250 x 4.6 mm; 25% - 100% over 10 min with 0.1 M ammomum acetate, lmL/min) R_ 6.75 min.
MS: MH4" 444

Example 464
Cw-4-[4-amino-5-(4-phenoxyphenyl)-7_H-pyrrolo[2,3-rf]pyrimidin-7-yl]-l-2- [(l__f-2-imidazolylmethyl)amino] ethyl-1-cyclohexanol diacetate
A mixture of ct5-l-(2-aminoethyl)-4-[4-amino-5-(4-ρhenoxyphenyl)-7H-ρynolo[2,3-Jlpyrimidin-7-yl]-l-cyclohexanol (0.192 g, 0.43 mmol), 1H-2-imidazolecarbaldehyde
(0.042 g, 0.43 mmol), sodium triacetoxyborohydride (0.119 g, 0.56 mmol) and acetic acid (0.052 g, 0.87 mmol) in 1,2-dichloroethane (25 ml) was heated at 40°C for one hour, after which time additional sodium triacetoxyborohydride (0.119 g, 0.56 mmol) was added. The mixture was stined at 40°C for two days. The solvent was removed in vacuo and the residue was partitioned between saturated aqueous sodium bicarbonate (50 mL) and dichloromethane (25 mL). The layers were separated, and the aqueous layer was extracted with dichloromethane (3x 25 mL). The combined organic layers were dried over magnesium sulfate and the solvent was removed in vacuo. The residue was purified by preparative RP-ΗPLC (Rainin C18, 8μm, 300 A, 25 cm; 30%> isocratic for five minutes, then 30%>-60% acetonitrile - 0.1 M ammonium acetate over 30 min, 21 ml/min). The solvent was removed and the residue was subjected to a second purification by preparative RP-ΗPLC using identical conditions. The organics were removed and the aqueous mixture was lyopholyzed to give ct_.-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-7-yl]-l-2-[(lH-2-imidazolylmethyl)amino]ethyl-l-cyclohexanol diacetate as a white solid (0.020 g, 0.03 mmol):
'Η NMR (DMSO-_E,400MΗz) δ 8.12 (s, IH), 7.48 (d, 2H), 7.42 (t, 2H), 7.34 (s, 1H), 7.16 (t, IH), 7.08-1.10 (m, 4H), 6.90 (bs, 2H), 6.09 (bs, IH), 4.52-4.58 (m, IH), 3.69 (s, 2H), 2.67-2.70 (m, 2H), 2.11-2.17 (m, 2H), 1.90 (s, 6H), 1.69-1.71 (m, 4H), 1.53-1.62 (m, 2H), 1.42-1.51 (m, 2H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) R_ 13.55 min.;
MS: M4" 524.

Example 465
Cis-2- {4- [4-amino-5-(4-phenoxyphenyl)-7_H-pyrr olo [2,3-<fl py rimidin-7-yl] -1 -hydroxycyclohexyl}-acetic acid
A mixture of ct_.-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-7-yl]-l-hydroxycyclohexylmethyl cyanide (0.172 g, 0.39 mmol) in dioxane (2 ml) was reacted with 2 M aqueous sodium hydroxide (2 mL) and 30%> hydrogen peroxide (3 drops) at reflux for ten days. The mixture was cooled to ambient temperature and adjusted to pΗ7 with 5%> aqueous citric acid. The solvent was removed in vacuo and the residue was purified by preparative RP-LC/MS ( Gilson-Micromass C18, 5μm, 130A, 21 cm, 0%>-100%> acetonitrile-0.1 M ammonium acetate over 9 min, 25 mL/min). The solvent was removed and the residue was subjected to a second purification by preparative RP-HPLC (Rainin C18, 8μm, 300 A, 25 cm; 35%-80% acetonitrile - 0.1 M ammonium acetate over 20 min, 21 ml/min). The acetonitrile was removed in vacuo and the aqueous mixture was lyopholyzed to give to give ct_?-{2-4-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-Jlpyrimidin-7-yl]-l-hydroxycyclohexylacetic acid as a white solid (0.008 g, 0.02 mmol):
Η NMR (DMSO-d6t 400MΗz) δ 11.94 (b, IH), 8.13 (s, IH), 7.48 (d, 2H), 7.41 (t, 2H), 7.35 (s, IH), 7.16 (t, IH), 7.08-7.10 (m, 4H), 6.09 (b, 2H), 4.51-4.57 (m, 2H), 2.39 (s, 2H), 2.10-2.28 (m, 2H), 1.79-1.90 (m, 2H), 1.62-1.78 (m, 4H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) R^ 14.25 min.;
MS: M4" 459.

Example 466
Ci5-2-{4-[4-ammo-5-(4-phenoxyphenyl)-7_ff-pyrrolo[2,3-< ]pyrimidin-7-yl]-l-hydroxycyclohexyl}-acetamide
A mixture of ct_.-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-J]pyι_imidin-7-yl]-l -hydroxy cyclohexy lmethyl cyanide (0.150 g, 0.34 mmol) and potassium carbonate (0.200g, 1.45 mmol) in dimethylsulfoxide (3 ml) was stined rapidly and 30% hydrogen peroxide (0.5 mL) was added dropwise, keeping the temperature at 20° C. The mixture was stined for seven hours at ambient temperature. Water (9 mL) was added, and the precipitate which formed was filtered, washing with water. The precipitate was purified by preparative RP-ΗPLC (Rainin C18, 8μm, 300 A, 25 cm; 30% isocratic for five minutes, then 30%>-60% acetonitrile - 0.1M ammomum acetate over 30 min, 21 ml/min). The acetonitrile was removed in vacuo and the aqueous mixture was lyopholyzed to give c._.-2-{4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-cT]pyrimidin-7-yl]-l-hydroxy-cyclohexyl}-acetamide as a white solid (0.040 g, 0.09 mmol):
Η NMR (DMSO-d6t 400MΗz) δ 8.13 (s, IH), 7.48 (d, 2H), 7.42 (t, 2H), 7.36 (s, IH), 7.16 (t, IH), 7.05-7.12 (m, 4H), 6.09 (b, 2H), 4.98 (s, IH) 4.51-4.59 (m, IH), 2.24 (s, 2H), 2.13-2.23 (m, 2H), 1.91 (s, IH), 1.72-1.77 (m, 4H), 1.57-1.65 (m, 2H); RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) R, 14.34 min.;
MS: M+458.

Example 467
C«-4-[4-amino-5-(4-phenoxyphenyl)-7_ -pyrrolo[2,3-rf]pyrimidin-7-yl]-l-(hydroxy-methyl)-l-cyclohexanol
A mixture of ct5-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_i]pyrimidin-4-amine (0.100 g, 0.24 mol) in 1,2-dimethoxyethane (3 mL) was reacted with 2.5 M aqueous potassium hydroxide (3 mL). The mixture was stined at reflux for eighteen hours. The mixture was cooled to room temperature and water (10 mL) was added. The precipitate which formed was filtered, washing with water (20 mL). The precipitate was purified by preparative RP-HPLC (Rainin C18, 8μm, 300 A, 25 cm; 30%> isocratic for five minutes, then 30%-60% acetonitrile - 0.1M ammonium acetate over 30 min, 21 ml/min). The acetonitrile was removed in vacuo and the aqueous mixture was lyopholyzed to give to give cts-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo [2,3 -_i]pyrimidin-7-yl] - 1 -(hydroxymethyl)- 1 -cyclohexanol as a white solid (0.012 g, 0.03 mol):
Η NMR (DMSO-_f6,400MΗz) δ 8.13 (s, IH), 7.49 (d, 2H), 7.42 (t, 2H), 7.36 (s, IH), 7.16 (t, IH), 7.05-7.12 (m, 4H), 6.08 (b, IH), 4.48-4.61 (m, 2H), 4.12 (s, IH), 3.22 (d, 2H), 2.10-2.25 (m, 2H), 1.72-1.76 (m, 2H), 1.55-1.70 (m, 4H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rt 14.31 min.;
MS: M4" 431.

General Procedure for Epoxide Opening by Amines
A mixture of 7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pyπolo[2,3- _ jpyrimidin-4-amine (0.075 g, 0.18 mmol) and amine (0.54 mmol) in 25%> dimethylformamide/iso-propanol (2.5 mL) was heated at 80 °C for 8 hours. The mixture was allowed to cool to ambient temperature and the solvent was removed under reduced pressure. The residue was purified by preparative ΗPLC -MS
(Hypersil 100 x 21.2 mm; 5 μ BDS C18, 25 mL/min; 0 - 70 %> over 8 min; 0.05 M ammonium acetate / acetonitrile; Micromass Mass spec). The solvent ofthe collected fractions was removed under the reduced pressure. The residue was dissolved in H2O and lyophilized for 24 hours.

Compounds prepared from ct_.-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3-__]pyrimidin-4-amine and the conesponding amine:

Example 468
Os-l-(aminomethyl)-4-[4-amino-5-(4-phenoxyphenyl)-7iϊ-pyrrolo[2,3- _/jpyrimidin-7-yl]-l-cyclohexanol
Η NMR (Chloroform-_i, 400 MHz) δ 8.19 (s, IH), 7.34 (m, 4H), 7.10 (t, IH), 7.03 (m, 5H), 5.74 (br, 2H), 4.65 (br, IH), 2.85 (s, 2H), 2.17 (br, 2H), 1.93 (br, 4H), 1.56

(br, 2H).
RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rt 13.0 min.
MS: MIT 430

Example 469
C/s-4-[4-amino-5-(4-phenoxyphenyl)-7_H-pyrrolo[2,3-rf]pyrimidin-7-yl]-l- [(dimethylamino)methyI]-l-cyclohexanoI
Η NMR (Chloroform-ci, 400 MHz) δ 8.32 (s, IH), 7.40 (m, 4H), 7.16 (t, IH), 7.10 (m, 4H), 7.00 (s, IH), 5.30 (br, 2H), 4.77 (br, IH), 2.58 (s, 2H), 2.41 (s, 6H), 2.09

(br, 2H), 1.93 (br, 4H), 1.83 (br, 2H).
RP-HPLC (Hypersil C18, 5μn, 250 x 4.6 mm; 25% - 100% over 10 min with 0.1 M ammonium acetate, lmL/min) Rj 7.28 min.
MS: MH+ 458

Example 470
Cw-2-[(4-[4-amino-5-(4-phenoxyphenyl)-7J_/-pyrrolo[2,3-rf]pyrimidin-7-yl]-l-hydroxy cyclohexy lmethyl)amino]-l,3-propanediol
Η NMR (Chloroform-./, 400 MHz) δ 8.27 (s, IH), 7.40 (m, 4H), 7.15 (t, IH), 7.10 (m, 5H), 5.24 (br, 2H), 4.65 (br, IH), 3.70 (m, 4H), 2.75 (m, IH), 2.65 (s, 2H), 2.20

(m, 2H), 2.12 (m, 4H), 1.55 (m, 2H).
RP-HPLC (Hypersil C18, 5 _m, 250 x 4.6 mm; 25% - 100% over 10 min with 0.1 M ammonium acetate, lmL/min) R_ 6.87 min.
MS: MET 504

Example 471
Cw-4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-rf]pyrimidin-7-yl]-l-[(2-morpholinoethyl)amino]methyl-l-cyclohexanol
Η NMR (Chloroform-./, 400 MHz) δ 8.31 (s, IH), 7.42 (m, 4H), 7.10 (m, 6H), 5.11 (br, 2H), 4.70 (br, IH), 3.73 (br, 4H), 2.85 (m, 2H), 2.65 (s, 2H), 2.54 (m, 2H), 2.49

(br, 4H), 2.21 (br, 2H), 1.96 (br, 2H), 1.85 (br, 2H), 1.58 (br, 2H).
RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammomum acetate over 20 min, lmL/min) Rj 13.6 min.
MS: MH4" 543

Compounds preprared from trαras-7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)- 7H-pynolo [2,3 -_ ]pyrimidin-4-amine

Example 472
r «s-l-(aminomethyl)-4-[4-amino-5-(4-phenoxyphenyl)-7_fi -pyrrolo[2,3-_/]pyrimidin-7-yl]-l-cyclohexanol diacetate
Η NMR (Chloroform-,/, 400 MHz) δ 8.19 (s, IH), 7.40 (m, 4H), 7.14 (t, IH), 7.07 (m, 5H), 5.91 (br, 2H), 4.70 (br, IH), 3.14 (s, 2H), 2.00 (br, 6H), 1.92 (br, 2H). RP-HPLC (Delta Pak C 18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rt 12.7 min.

Example 473
ϊ> «s- 4-[4-amino-5-(4-phenoxyphenyl)-7_iϊ-pyrrolo[2,3-_ ]pyrimidin-7-yl]-l-[(dimethylamino)methyl]-l-cyclohexanol
Η NMR (Chloroform-./, 400 MHz) δ 8.26 (s, IH), 7.40 (m, 4H), 7.15 (t, IH), 7.10 (m, 5H), 5.31 (br, 2H), 4.70 (br, IH), 3.11 (s, 2H), 2.76 (s, 6H), 2.10 (br, 6H), 1.87 (br, 2H).
RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rt 13.3 min.
MS: MH4" 458

Example 474
rr «s-2-[(4-[4-amino-5-(4-phenoxyphenyl)-7JE/-pyrrolo[2,3-_/]pyrimidin-7-yl]-l-hydroxycyclohexylmethyl)amino]-l,3-propanediol
Η NMR (Chloroform-./, 400 MHz) δ 8.17 (s, IH), 7.35 (m, 4H), 7.15 (t, IH), 7.06

(m, 5H), 6.20 (br, 2H), 4.70 (br, IH), 3.89 (m, 4H), 3.34 (s, 2H), 3.27 (br, IH), 2.10

(br, 6H), 1.90 (br, 2H).
RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rj 12.8 min.
MS: MH4" 504

Example 475
Trans-4- [4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo [2,3-./] py rimidin-7-yl] -1 - [(2-morpholinoethyl)amino]methyl-l-cyclohexanol
Η NMR (Chloroform-,/, 400 MHz) δ 8.30 (s, IH), 7.42 (m, 4H), 7.10 (m, 5H), 6.99 (s, IH), 5.46 (br, 2H), 4.70 (br, IH), 3.71 (br, 4H), 2.91 (s, 2H), 2.86 (m, 2H), 2.55 (m, 2H), 2.44 (br, 4H), 2.10 (br, 2H), 1.90 (br, 4H), 1.80 (br, 2H).
RP-HPLC (Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) R_ 13.6 min.

MS: MH4" 543

Compounds prepared from a mixture of cis- and trα -7-(l-oxaspiro[2.5]oct-6-yl)-5-(4-phenoxyphenyl)-7H-pynolo[2,3--/]pyrirm-din-4-amine (ratio cis/trans 2:1) and the conesponding amine (RP-ΗPLC (Delta Pak Cl 8, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammomum acetate over 20 min, lmL/min))

Example 476

4-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-c?]pyrimidin-7-yl]-l-[(2-hydroxyethyl)amino]methyl- 1 -cyclohexanol
ΗPLC: Rt 12.9 min, 13.1 min
MS: M"=474

Example 477
4-[4-amino-5-(4-phenoxyphenyl)-7_J-pyrroIo[2,3-t ]pyrimidin-7-yl]-l-[(3-hydroxypropyl)amino]methyl-l-cyclohexanol
HPLC: Rt 13.1 min
MS: M+=488

Example 478
4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-</]pyrimidin-7-yl]-l-([3-(lHr-l-imidazolyl)propyIJaminomethyl)-l-cyclohexanol
HPLC: Rt 12.4 min, 12.8 min
MS: M+=538

Example 479
4-[4-amino-5-(4-phenoxyphenyI)-7H-pyrrolo[2,3-< ]pyrimidin-7-yl]-l-(lHr-l-imidazolylmethyl)-l-cyclohexanol HPLC: Rt 13.9
MS: M+=481

Example 480
4-[4-amino-5-(4-phenoxyphenyl)-7_J-pyrrolo[2,3-</]pyrimidin-7-yl]-l-([2-(2-hydroxyethoxy)ethyl]aminomethyl)-l-cyclohexanol
HPLC: Rt 13.1 min
MS: M+=518

Example 481
4-[4-amino-5-(4-phenoxyphenyl)-7J3-pyrrolo[2,3-< jpyrimidin-7-yl]-l-[di(2-hydroxyethyl)amino]methyl-l-cyclohexanol
HPLC: Rt 13.1 min, 13.3 min
MS: M+=518

Example 482
4-[4-ammo-5-(4-phenoxyphenyl)-7_9-pyrrolo[2,3-< ]pyrimidm-7-yl]-l-([2- (dimethylamino)ethyl] aminomethyl)-l-cyclohexanoI
HPLC: Rt 12.9 min, 13. min
MS: M+=501

Example 483
4-[4-amino-5-(4-phenoxyphenyl)-7_?_r-pyrrolo[2,3-< |pyrimidin-7-yl]-l-[(4-methylpiperazino)methyl]-l-cyclohexanol
HPLC: Rt 13.7 min, 14.0 min
MS: M+=513

Example 484
4-[4-amino-5-(4-phenoxyphenyl)-7_f_T-pyrrolo[2,3-__]pyrimidin-7-yl]-l-(morpholinomethyl)-l -cy clohexanol
HPLC: Rt 13.9 min, 14.0 min MS: M+=500

Example 485
l_(4-[4-amino-5-(4-phenoxyphenyl)-7_H-pyrrolo[2,3-rf]pyrimidin-7-yl]-l-hydroxycyclohexylmethyl)-4-piperidinol
HPLC: Rt 13.2 min, 13.4 min
MS: M+=514

Example 486
4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-< lpyrimidin-7-yl]-l-[methyl(l-methyI-4-piperidyl)amino]methyl-l-cyclohexanol
HPLC: Rt 12.5 min, 13.0 min
MS: M+=541

Example 487
4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3-rf]pyrimidin-7-yl]-l-[(3-morpholinopropyl)amino]methyl-l-cycIohexanol
HPLC: Rt 12.8 min, 13.1 min
MS: M+=557

Example 488
4-[4-amino-5-(4-phenoxyphenyl)-7_Hr-pyrrolo[2,3-έ/]pyrimidin-7-yl]-l-[(2-piperidinoethyl) amino] methyl-1 -cyclohexanol
HPLC: Rt 13.4 min, 13.6 min
MS: M4=541

Example 489
4-[4-amino-5-(4-phenoxyphenyl)-7_H-pyrroIo[2,3-( ]pyrimidin-7-yl]-l-([3- (diethylamino)propyl]aminomethyl)-l-cyclohexanol
HPLC: Rt 12.5 min, 13.0 min
MS: M+=543 Example 490
4-[4-amino-5-(4-phenoxyphenyl)-7_H-pyrrolo[2,3-<f]pyrimidm-7-yl]-l- [(tetrahydro-2-furanylmethyl)amino]methyl-l-cyclohexanol
HPLC:Rt 14.2 min
MS: M+=514

Example 491
Cs-8-[4-amino-5-(4-phenoxyphenyl)-7_H-pyrrolo[2,3-</]pyrimidin-7-yl]-l,3-diazaspiro [4.5] decane-2,4-dione.
A mixture of 4-[4-__mmo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_f]pyrimidin-7-yl]-l-cyclohexanone (0.500 g, 0.00125 mol), ammomum carbonate (0.602 g, 6.27 mmol), and potassium cyanide (0.163 g, 2.51 mmol) in ethyl alcohol (5 mL) and water (5 mL) was stined 18 hours at 60°C, after which time all material had gone into solution. The solvents were removed in vacuo and the residue was partitioned between ethyl acetate (80 mL) and water (20 mL). The phases were separated and the organic phase was washed with brine (20 mL). The organic phase was dried over magnesium sulfate, and the solvent was removed in vacuo. The crude material had a cis: trans ratio of 7:1. The product was purified by flash column
chromatography on silica using dichloromethane /methanol (90: 10). The solvent was removed in vacuo to give cts-8-[4-amino-5-(4-phenoxyphenyl)-7H-pyπolo[2,3-_ ]pyrimidin-7-yl]-l,3-diazaspiro[4.5]decane-2,4-dione as a white solid (0.183 g, 0.39 mmol):
Η NMR (DMSO-_/6, 400MΗZ) δ 10.71 (s, IH), 8.64 (s, IH), 8.15 (s, IH), 7.48 (d, 2H), 7.42 (t, 2H), 7.16 (t, IH), 7.12 (d, 2H), 7.09 (d, 2H), 6.13 (b, 2H), 4.73-4.79 (m, IH), 2.00-2.10 (m, 2H), 1.88-1.94 (m, 4H), 1.71-1.74 (m, 2H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rj 15.04 min.
MS: M+ 469.

Example 492
Cis-S- [4-amino-5-(4-phenoxyphenyl)-7_ff-pyrrolo [2,3-d\ pyrimidin-7-yl] -1 ,3-diazaspiro [4.5] decan-2-one
A mixture of cw-8-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrimidin-7-yl]-l,3-diazaspiro[4.5]decane-2,4-dione (0.350 g, 0.75 mmol) in tefrahydrofuran (20 mL) was stined at room temperature and lithium aluminum hydride (0.057 g, 1.49 mmol) was added over a 10 minute time period at ambient temperature. Gas evolution was observed, and the mixture was stined for 18 hours at 50° C.
Additional lithium aluminum hydride (0.057 g, 1.49 mmol) was added and the mixture was heated at reflux for 24 hours. Water (10 mL) was added, followed by 15% aqueous sodium hydroxide (10 mL). The mixture was stined 30 minutes and additional water (15 mL) was added. The mixture was filtered through a pad of Celite © 521. The filtrate was extracted with ethyl acetate (3 x 25 mL). The combined organic layers were dried over magnesium sulfate and the solvent was removed in vacuo. The residue was purified by by flash column chromatography on silica using dichloromethane/methanol (95:5) as an eluent. The solvent was removed in vacuo to give ct_f-8-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]-l,3-diazaspiro[4.5]decan-2-one as a white solid (0.045 g, 0.10 mmol):
Η NMR (DMSO- 6,400MΗz) δ 8.12 (s, IH), 7.64 (s, IH), 7.47 (d, 2H), 7.42 (t, 2H), 7.14 (t, IH), 7.11 (d, 2H), 7.09 (d, 2H), 6.18 (s, IH), 6.08 (b, 2H), 4.66-4.71 (m, IH), 3.09 (s, 2H), 2.06-2.11 (m, 2H), 1.76-1.81 (m, 4H), 1.63-1.69 (m,2H); RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1
M ammonium acetate over 20 min, lmL/min) Rt 15.11 min.;
MS: M+455.

Example 493
C _,-4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3--/lpyrimidin-7-yl]-l-ammoniocyclohexylmethanol acetate

a.) l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7J3-pyrrolo[2,3-< ]pyrimidin-7-yl]- 1-cyclohexanecarboxylic acid
A mixture of cis- and trαn_.-8-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-d]pyrimidin-7-yl]-l,3-diazaspiro[4.5]decane-2,4-dione (0.600 g, 1.3 mmol, ratio cis/trans 7:1) in 2 M aqueous sodium hydroxide (25 mL) was heated at reflux for seven days. The mixture was cooled to room temperature and brought to pΗ 8 by drop-wise addition of concenfrated hydrochloric acid. The precipitate which formed was collected by filfration and washed with water. The precipitate was triturated with methanol (50 mL) for 18 hours. The mixture was filtered, and the solvent was removed from the filfrate in vacuo to give l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3--f|pyrimidin-7-yl]-l-cyclohexanecarboxylic acid (0.194 g, 0.43 mol) which was used without further purification.:
'Η NMR (DMSO-_/400MΗz) δ 8.12-8.14 (m, IH), 7.37-7.55 (m, 5H), 7.05-7.11 (m, 5H), 6.08 (b, 2H), 4.47-4.53 (m, IH), 4.10 (b, 3H), 1.35-1.2.22 (m, 8H); RP-HPLC (Hypercil C18, 5μm, 100 A, 15 cm; 5%-100% acetonitrile - 0.1M
ammomum acetate over 15 min, lmL/min) Rt 10.12 min.; MS: M+444.

6.) Methyl l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7_ff-pyrrolo[2,3-</]pyrimidin-7-yl]-l-cyclohexanecarboxylate
A mixture of cis- and tr_.«_.-l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_/]pyrimidin-7-yl]-l-cyclohexanecarboxylic acid (0.696 g, 1.6 mmol, ratio cis/trans 7:1) was suspended in methanol (50 mL). The mixture was cooled to 0° C and thionyl chloride (0.211 g, 0.1.77 mmol) was added dropwise. After the addition was complete, the mixture was heated at reflux for three days, with addition of an additional equivalent of thionyl chloride each day. The solvent was removed in vacuo to give crude methyl l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3--/]pyrimidin-7-yl]-l-cyclohexanecarboxylate (1.171 g) which was carried forward without further purification.:
Η MR (DMSO-_/6,400MΗz) δ 9.08 (b, 2H), 8.53 (s, IH), 8.30 (s, IH), 7.51 (d, 2H), 7.43 (t, 2H), 7.18 (t, IH), 7.10-7.15 (m, 4H), 4.82-4.98 (m, IH), 3.17 (s, 3H), 2.05-2.28 (m, 4H), 1.86-1.98 (m, 2H);

RP-HPLC (Perkin-Elmer Pecosphere C18, 3μm, 100 A, 33x4.6 mm; 0%-100% acetonitrile - 0.1 M ammonium acetate; 3-3.5 mL/min over 4.5 min, then 100% acetonitrile at 3 mL/min over 0.5 min) R^ 2.81 min.;
MS: M+458.

c.) C5-4-[4-amino-5-(4-phenoxyphenyl)-7H-pyrrolo[2,3- _]pyrimidin-7-yl]-l-ammoniocyclohexylmethanol acetate
A crude mixture of cis-and trans-ethyl l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]-l-cyclohexanecarboxylate (1.17 g,) in
tetrahydrofuran (75 mL) was stined at room temperature and lithium aluminum hydride (0.18 g, 4.7 mmol) was added over a 10 minute time period at ambient temperature. Gas evolution was observed, and the mixture was stined for 18 hours at 40° C. Water (5 mL) was added, followed by 15%> aqueous sodium hydroxide (15 mL) and additional water (15 mL). The mixture was filtered through a pad of Celite ® 521. The filtrate was exfracted with ethyl acetate (3 x 25 mL). The combined organic layers were dried over magnesium sulfate and the solvent was removed in vacuo. The residue was purified by preparative RP-ΗPLC (Rainin C18, 8μm, 300 A, 25 cm; 35%r80%> acetonitrile - 0.1 M ammonium acetate over 10 min, 21 ml/min). The acetonitrile was removed in vacuo and the aqueous mixture was lyopholyzed to give ct1s-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-7-yl]- 1 -ammoniocyclohexylmethanol acetate as a white solid (0.577 g, 1.16 mmol):
Η NMR (DMSO-_/400MΗz) δ 8.13 (s, IH), 7.55 (s, IH), 7.48 (d, 2H), 7.42 (t, 2H), 7.14 (t, IH), 7.11 (d, 2H), 7.09 (d, 2H), 6.08 (b, IH), 4.56-4.65 (m, IH), 3.29 (s, 2H), 2.13-2.26 (m, 2H), 1.80 (s, 3H), 1.50-1.79 (m, 8H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammomum acetate over 20 min, lmL/min) Rt 12.92 min.;
MS: M+430.

Example 494 and 495
Cis- and trøns-7-[4-amino-4-(ammoniomethyl)cyclohexyl]-5-(4-phenoxyphenyl)- 7_J-pyrrolo[2,3-._]pyrimidin-4-amine acetate

a.) Cis- and trαλ_s-l-ammo-4-[4-ammo-5-(4-phenoxyphenyl)-7_f_T-pyrroIo[2,3-r/]pyrimidin-7-yl]-l-cyclohexanecarbonitrile.
A mixture of 4-[4-ammo-5-(4-phenoxyphenyl)-7H-pynolo[2,3-^pyrinndin-7-yl]-l-cyclohexanone (3.24 g, 8.1 mmol), potassium cyanide (1.06 g, 0.0162 mol), and ammomum chloride (0.869 g, 16.2 mmol) in ammomum hydroxide (32 ml) and ethyl alcohol (26 mL) was heated at 50°C for eighteen hours. Water (150 mL) was added and the mixture was- exfracted with dichloromethane (3 x 60 mL). The combined organic layers were dried over magnesium sulfate and the solvent was removed in vacuo to give a mixture of cis and trα«s-l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo [2,3 -t ]pyrimidin-7-yl] - 1 -cyclohexanecarbonifrile as an amorphous white solid (3.17 g, 7.5 mmol):
Η NMR (DMSO-_/6,400MΗz) δ 8.14 (s, 0.7 H), 8.13 (s, 0.3 H), 7.45-7.51 (m, 2H), 7.38-7.44 (m, 3H), 7.13-7.18 (m, IH), 7.06-7.12 (m, 4H), 6.11 (b, IH), 4.60-4.68 (m, IH), 2.19-2.28 (m, 2H), 1.97-2.10 (m, 4H), 1.72-1.76 (m, 2H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1M ammonium acetate over 20 min, lmL/min) Rj 15.98, 16.38 min.;
MS: M+425.

b.) C 5-7-[4-ammo-4-(ammoniomethyl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pyrrolo [2,3-rf] py rimidin-4-amine acetate
A mixture of cis and tr__7.5-l-amino-4-[4-amino-5-(4-phenoxyphenyl)-7H-pynolo[2,3-<i]pyrimidin-7-yl]-l-cyclohexanecarbonifrile (1.00 g, 2.4 mmol) in tefrahydrofuran (75 mL) was stined at room temperature and lithium aluminum hydride (0.16 g, 4.2 mmol) was added over a 10 minute time period at ambient temperature. Gas evolution was observed, and the mixture was stined for 18 hours. Water (5 mL) was added, followed by 15% aqueous sodium hydroxide (15 mL) and additional water (15 mL). The mixture was filtered through a pad of Celite ® 521. The filfrate was extracted with ethyl acetate (3 x 15 mL). The combined organic layers were dried over magnesium sulfate and the solvent was removed in vacuo. The isomers were separated by preparative RP-HPLC (Rainin C18, 8μm, 300 A, 25 cm; 30% isocratic for five minutes, then 30%o-60% acetonitrile - 0.1 M ammonium acetate over 30 min, 21 ml/min). The acetonitrile was removed from the less polar fraction and this fraction was lyopholyzed to give cz_.-7-[4-amino-4- (ammoniomethyl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3- ]pyrimidin-4-amine acetate as a white solid (0.137 g, 0.28 mmol):
Η NMR (DMSO-./6I 400MΗZ) δ 8.13 (s, 1Η), 7.48 (d, 2Η), 7.41 (t, 2H), 7.41 (s, IH), 7.16 (t, IH), 7.10 (d, 2H), 7.08 (d, 2H), 6.08 (b, IH), 4.52-4.58 (m, IH), 2.71-2.79 (m, IH), 1.80-1.95 (m, 6H), 1.30-1.36 (m, 2H);
RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%-85% acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) Rt 13.31 min.;
MS: M4" 429.

c.) r «s-7-[4-amino-4-(ammoniomethyl)cyclohexyl]-5-(4-phenoxyphenyl)-7__T-pyrrolo[2,3-rf]pyrimidin-4-amine acetate
The solvents were removed in vacuo from the more polar fraction from the above procedure. The residue was subjected to a second purification by preparative RP-HPLC (Rainin C18, 8μm, 300 A, 25 cm; 20%>-60% acetonitrile - 0.1M ammonium acetate over 30 min, 21 ml/min). Acetonitrile was removed in vacuo and the aqueous solution was lyopholyzed to give tra7._.-7-[4-amino-4-(ammoniomethyl)cyclohexyl]-5-(4-phenoxyphenyl)-7H-pynolo[2,3-_ ]pyrimidin-4-amine acetate as a white solid (0.037 g, 0.00008 mol):
Η NMR (DMSO-J6j 400MΗz) δ 8.13 (s, IH), 7.45-7.54 (m, 3H), 7.42 (t, 2H), 7.16 (t, IH), 7.06-7.13 (m, 4H), 6.09 (b, IH), 4.52-4.60 (m, IH), 1.91-2.07 (m, 2H), 1.86 (s, 3H), 1.71-1.84 (m, 4H), 1.40-1.53 (m, 2H); RP-HPLC ( Delta Pak C18, 5μm, 300A, 15 cm; 5%>-85%> acetonitrile - 0.1 M ammonium acetate over 20 min, lmL/min) R,, 12.18 min.; MS: M+429.

Example 496
5-[4-(benzyloxy)phenyl]-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7_H-pyrrolo[2,3- <flpyrimidin-4-amine

l,4-dioxaspiro[4.5]decan-8-ol
A solution of l,4-dioxaspiro[4.5]decan-8-one (150 g, 962 mmol) in methanol (1 L) was freated with sodium borohydride (36.38 g, 962 mmol) portion-wise over 2 hours at 0°C under a nitrogen atmosphere. The ice bath was removed after 2 hours, and the reaction mixture stined over night at room temperature. After 24 hours, the solvent was removed under reduced pressure, dichlorormethane:iso-propanol (3:1, 1 L) solution and 2 N sodium hydroxide aqueous solution(400 mL) were added. The aqueous layer was exfracted with the dichloromethanetiso-propanol solution (1 L). The organic layers were combined, washed water (500 mL), dried over magnesium sulfate, filtered and concentrated under reduced pressure. Residual solvent was removed under high vacuum pressure to give 144.18 g (94 %) of the product 1 ,4-dioxaspiro[4.5]decan-8-ol. Η NMR (DMSO-d6, 400 MHz) δ 4.46-4.45 (d, IH, J= 4 Hz), 3.83 (s, 4H), 3.55 (br s, IH), 1.69-1.64 (m, 4H), 1.49-1.40 (m, 4H). TLC (ethyl acetate / heptane = 1:3, stain potassium permanganate) Rf = 0.2

4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-5-iodo-7 f-pyrroIo[2,3-d]pyrimidine
A solution of 4-chloro-5-iodo-7H-pynolo[2,3-- ]pyrimidine (25 g, 89.46 mmol) in tefrahydrofuran (600 mL) was treated with l,4-dioxaspiro[4.5]decan-8-ol (42.45 g, 268.5 mmol), triphenylphosphine (46.95 g, 179 mmol), and
diethylazodicarboxyate (31.17 g, 179 mmol). The reaction mixture stined for 24 hours at room temperature under a nifrogen atmosphere. The solid, which precipitated, was filtered and washed with ethyl acetate. The reaction solution was partially evaporated under reduced pressure. A solid precipitated and was filtered. The product contained reduced DEAD. Ethyl acetate (50- 100 mL) was added to the solid. The product is not soluble in ethyl acetate, and was filtered. The xrituration yielded 23.69 g (63 %>) of 4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-5- iodo-7H-pynolo[2,3-_ ]pyrimidine. Η NMR (DMSO-d6, 400 MHz) δ 8.64 (s, IH), 8.10 (s, IH), 4.77-4.71 (m, IH), 3.95-3.88 (m, 4H), 2.17-2.08 (m, 2H), 1.93-1.90 (d, 2H, J= 12 Hz), 1.79-1.70 (m, 4H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 μm, 2.1 x 50 mm; 5%)-95%o acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) R_ 6.99 min.

5-[4-(benzyloxy)phenyl]~4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pyrrolo[2,3-- Jpyrimidme
A mixture of 4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-5-iodo-7H-pyπolo[2,3-_Z]pyrimidine (3.0 g, 7.15 mmol) in ethylene glycol dimethyl ether (100 mL) was freated with 4-(benzyloxy)phenylboronic acid (1.79 g, 7.87 mmol), tefrakis(triphenylphosphine)palladium (0.496 g, 0.429 mmol), and a solution of sodium carbonate (1.83 g, 17.16 mmol) in water (50 mL). A precipitate formed after 20 minutes. Reaction was stined for 5 hours at 80°C under a nifrogen atmosphere. The mixture was allowed to cool to room temperature and the organic solvent was removed under reduced pressure. The product was partitioned between the aqueous sodium carbonate layer and ethyl acetate. The aqueous layer was further extracted with ethyl acetate three times. The organic layers were combined, washed with water and brine, dried over magnesium sulfate, and partially concentrated until a precipitate formed. The solid was filtered, washed with ethyl acetate, and dried under a high vacuum. Yielded 2.06 g (61%>) of 5-[4-(benzyloxy)phenyl]-4-chloro-7-(l,4-dioxasρiro[4.5]dec-8-yl)-7H-ρynolo[2,3-t/]pyrimidine. Η NMR (DMSO-d6, 400 MHz) δ 8.64 (s, IH), 7.88 (s, IH), 7.52-7.33 (m, 7H), 7.08-7.05 (d, 2H, J= 12 Hz), 5.15 (s, 2H), 4.82-4.78 (m, IH), 3.95-3.89 (m, 4H), 2.21-2.13 (m, 2H), 1.98-1.96 (m, 2H), 1.85-1.75 (m, 4H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 μm, 2.1 x 50 mm; 5%-95%> acetonitrile-0.1 M ammomum acetate over 15 min, 0.5 mL/min) R^ 8.356 min.

5-[4-(benzyloxy)phenyl]-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7_H-pyrrolo[2,3-_/]pyrimidin-4-amine A mixture of 5-[4-(benzyloxy)phenyl]-4-chloro-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-_/]pyrimidine (1.925 g, 4.01 mmol) and concentrated ammonium hydroxide (50 ml) in dioxane (50 mL) was heated at 120°C in a pressure vessel for 20 hours. The reaction mixture was allowed to cool to room temperature. Ethyl acetate (300 mL) and saturated sodium chloride aqueous solution (300 mL) were added. The layers were partitioned and the aqueous layer was extracted with ethyl acetate (700 mL). The organic layers were combined, washed with water, dried over magnesium sulfate, and the solvent removed under reduced pressure. The compound was purified by flash chromatography on silica using 5%> methanol in dichloromethane to give 1.457 g (81%>) of 5-[4-(benzyloxy)phenyl]-7-(l,4-dioxasρiro[4.5]dec-8-yl)-7H-pynolo[2,3-- ]ρyrimidin-4-amine. lH NMR (DMSO-d6, 400 MHz) δ 8.13 (s, IH), 7.49-7.31 (m, 8H), 7.121-7.100 (d, 2H, J= 8Hz), 6.02 (br s, 2H), 5.149 (s, 2H), 4.681-4.620 (m, IH), 3.94-3.88 (m, 4H), 2.12-2.03 (m, 2H), 1.93-1.90 (m, 2H), 1.82-1.71 (m, 4H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 μm, 2.1 x 50 mm; 5%-95% acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rt 6.924 min.

Examples 497 and 498
C«-5-[4-(ben__yloxy)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7_J-pyrrolo[2,3-</]pyrimidin-4-amine and r κs-5-[4-(benzyloxy)phenyl]-7-[4-(4-methylpiperazino)cycIohexyl]-7_?_r-pyrrolo[2,3-< ]pyrimidin-4-amine

4-{4-amino-5-[4-(benzyloxy)phenyl]-7_H-pyrrolo[2,3-rf]pyrimidin-7-yl}-l-cyclohexanone
A suspension of 5-[4-(benzyloxy)phenyl]-7-(l ,4-dioxaspiro[4.5]dec-8-yl)-7H-pynolo[2,3-cf]pyrimidin-4-amine (1.447 g, 3.06 mmol) in acetone (100 mL) was treated with 5 N hydrochloric acid aqueous solution (25 mL) drop-wise over 10 min at 0°C under a nifrogen atmosphere. The reaction mixture was stined at 0°C for 30 minutes and ice bath was removed. The reaction mixture was stined for 72 hours then the organic layer was removed under reduced pressure. The aqueous layer was basified to approximately pH 8 with 50 % sodium hydroxide aqueous solution. The aqueous layer was extracted with dichloromethane (600 mL). The organic layer was dried with magnesium sulfate, filtered, concenfrated under reduced pressure, and dried under high vacuum for 3 hours to give 1.221 g (94 %) of 4-4-amino-5-[4-(benzyloxy)phenyl]-7H-pynolo[2,3-_ ]pyrimidin-7-yl-l-cyclohexanone. 'Η NMR (DMSO-d6, 400 MHz) δ 8.15 (s, IH), 7.48-7.34 (m, 8H), 7.13-7.11 (d, 2H, J= 8H), 6.13-6.04 (br s, 2H), 5.19-5.15 (s, 3H), 2.79-2.67 (m, 2H), 2.33-2.30 (m, 4H), 2.30-2.20 (m, 2H); Waters 2690 Alliance HPLC (Symmetry Shield RP183.5 μm, 2.1 x 50 mm; 5%>-95% acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rt 6.51 min.

a. A suspension ofthe 4-{4-amino-5-[4-(benzyloxy)phenyl]-7H-pynolo[2,3-ύT]pyrimidin-7-yl}-l-cyclohexanone (1.22g, 2.96 mmol) in dichloroethane (40 ml) was freated with N-methylpiperazine (0.889g, 8.88 mmol) and glacial acetic acid (0.534 g, 8.88 mmol) at 0°C for 1 hour. Subsequently, sodium triacetoxyborohydride (0.816g, 3.85 mmol) was added and the reaction mixture was stined overnight. Additional N-methylpiperazine (0.445 g, 4.44 mmol), sodium triaceoxyborohydride (0.407 g, 1.92 mmol), and glacial acetic acid (0.267 g, 4.44 mmol) were added and the reaction mixture was stined for an additional 1.5 hour. To the reaction solution, a solution of sodium bicarbonate (1.49 g, 17.76 mmol) in 25 mL water was added and stined for 1 hour. The aqueous layer was extracted with ethyl acetate (4 x 125 mL), The organic layers were combined, washed with water and brine (each 4x 125 ml), dried over magnesium sulfate, filtered and concenfrated under reduced pressure to give 1.6 g of crude material. The compound was purified by flash
chromatography on silica gel using 10% methanol in dichloromethane, 20% methanol in dichloromethane, and then 30% methanol in dichloromethane. The column yielded 0.83 g (56%) of Cw-5-[4-(benzyloxy)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-<i]pyrimidin-4-amine, and 0.305g (21%) offr n5s-5-[4-(benzyloxy)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7-_y-pyrrolo[2,3--_]pyrimidin-4-amine.

C s-5-[4-(benzyloxy)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7_H-pyrrolo[2,3-^pyrimidin-4-amme:
Η NMR (DMSO-d6, 400 MHz) δ 8.12 (s, IH), 7.49-4.47 (m, 2H), 7.43-7.34 (m, 5 H), 7.20 (s, IH), 7.13-7.11 (d, 2H, J= 8 Hz), 6.1-5.9 (br s, 2H), 5.15 (s, 2H), 4.70-4.64 (m, IH), 2.5-2.3 (br s, 8H), 2.18 (s, 4H), 2.11-2.05 (m, 4H), 1.70-1.67 (m, 2H), 1.59-1.52 (m, 2H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 μm, 2.1 x 50 mm; 5%-95%> acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rt 5.161 min.

rrα«_.-5-[4-(benzyloxy)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-Oρyrimidin-4-amine Η NMR (DMSO-d6, 400 MHz) 8.11 (s,

IH), 7.48-7.46 (d, 2H, j = 7.6 Hz), 7.42-7.33 (m, 6H), 7.12-7.10 (d, 2H, j = 8.8 Hz), 6.1-5.9 (br s, 2H), 5.14 (s, 2H), 4.55-4.50 (m, IH), 2.6-2.4 (br s, 4H), 2.40- 2.25 ( m, 5H), 2.14 (s, 3H), 2.0-1.8 (m, 6H), 1.5-1.3 (m, 2H). Waters 2690 Alliance HPLC

(Symmetry Shield RP18 3.5 m, 2.1 x 50 mm; 5%-95% acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) R_ 5.129 min.

Example 499
4-amino-5-(4-phenoxyphenyI)-7-[l-(l-methyI-4-piperidinyl)-4-piperidinyl]-7H-pyrrolo[2,3-rf]pyrimidine

tert-butyl 4-hydroxy-l-piperidinecarboxylate
A solution of tert-butyl 4-oxo-l -piperidinecarboxylate (179.24 g, 899.62 mmol) in anhydrous methanol (1.5 L) at 0°C under a nifrogen atmosphere was treated with sodium borohydride (34.03 g, 899.62 mmol). Sodium borohydride was added portion-wise (5 g every 10 minutes). The ice bath was removed 20 minutes after the last addition of sodium borohydride, and the reaction mixture stined for 72 hours. To the reaction solution, 1 N sodium hydroxide aqueous solution (IL) was added, and allowed to stir for 2 hours. The organic layer was removed under reduced pressure, and anhydrous diethyl ether (200 mL) was added. The aqueous layer was exfracted with anhydrous diethyl ether (1.5 L). The organic layers were combined, washed with brine, dried over magnesium sulfate, filtered and evaporated under reduced pressure to give a yellow oil. The product was further dried under high vacuum to remove residual solvent to give 177.04 g (97%) of tert-butyl 4-hydroxy-1 -piperidinecarboxylate as a white solid. . lH NMR (DMSO-d6, 400 MHz) δ 4.688 (s, IH), 3.678-3.589 (m, 3H), 2.940 (m, 2H), 1.689-1.646 (m, 2H), 1.385 (s, 9H), 1.252-1.212 (m, 2H).

tert-butyl 4-(4-chloro-5-iodo-7H-pyrrolo[2,3-_ ]pyrimidin-7-yl)-l-piperidinecarboxylate

A solution of 4-chloro-5-iodo-7H-pynolo[2,3-_?/pyrimidine (6.48 g, 21.78 mmol) in tefrahydrofuran (200 mL) at 0°C under a nifrogen atmosphere was treated with tert-butyl 4-hydroxy- 1 -piperidinecarboxylate (13.147g, 65.33 mmol) and xriphenylphosphine (11.43 g, 43.56 mmol) and stined for 30 minutes.
Diethylazodicarboxyate (7.61 g, 43.56 mmol) was added drop-wise over 15 minutes to the reaction solution. The ice bath was removed after 15 minutes, and the reaction mixture was allowed to stir over night. The solvent was evaporated under reduced pressure, and then the yellow gummy solid dissolved in dichloromethane. The solid precipitated was filtered and discarded. The filtrate was evaporated under reduced pressure. Recrystallization from ethyl acetate / heptane yielded 4.82 g (47%) of tert-butyl 4-(4-chloro-5-iodo-7Η-pynolo[2,3-d]pyrinfrdin-7-yl)-l-piperidinecarboxylate. Η NMR (DMSO-d6, 400 MHz) 8.64 (s, IH), 8.20 (s, IH), 4.89-4.84 (m, IH), 4.13-4.10 (m, 2H), 3.00-2.80 (br s, 2H), 2.01-1.88 (m, 4H), 1.43 (s, 9H). Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 m, 2.1 x 50 mm; 5%-95% acetonitrile-0.1 M ammomum acetate over 15 min, 0.5 mL/min) Rj 7.825 minutes.

4-chloro-5-iodo-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidine

A mixture of tert-butyl 4-(4-chloro-5-iodo-7Η-pynolo[2,3-d]pyrimidin-7- yl)-l-piperidinecarboxylate (1.0 g, 2.16 mmol) in dichloromethane (10 mL) at 0°C was freated with a cold solution of trifluoroacetic acid (20%) in dichloromethane (total volume 56 mL). Reaction stined at 0°C under a nitrogen atmosphere for 3 hours. The solvent was evaporated under reduced pressure at ambient temperature. Ethyl acetate (50 mL) and 5 N hydrochloric acid (50 mL) were added to the solid. The layers were partitioned, and the organic layer was exfracted with 5 N
hydrochloric acid (150 mL). The aqueous layers were combined, cooled to 0°C, and basified using 50% sodium hydroxide aqueous solution at 0°C. The aqueous layer was extracted with dichloromethane (300 mL). The organic layers were combined, dried over magnesium sulfate, filtered, and evaporated under reduced pressure. The resulting product, 4-chloro-5-iodo-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidine (0.520 g, 99%) was dried under high vacuum. ]H NMR (DMSO-d6, 400 MHz) 8.63 (s, IH), 8.12 (s, IH), 4.75-4.68 (m, IH), 3.09-3.06 (d, 2H, J = 12 Hz), 2.70-2.60 (m, 2H), 1.99-1.83 (m, 4H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 m, 2.1 x 50 mm; 5%-95%> acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rj 4.022 min.

4-chloro-5-iodo-7-[l-(l-methyl-4-piperidinyl)-4-piperidinyl]-7H-pyrrolo[2,3-<Z]pyrimidine
A mixture of 4-chloro-5-iodo-7-(4-piperidinyl)-7H-pynolo[2,3-^pyrimidine

(0.52 g, 1.43 mmol), l-methyl-4-piperidone (0.178 g, 1.57 mmol), and glacial acetic acid (0.094 g, 1.57 mmol) in dichloroethane was stined at 0°C for 20 minutes. Sodium triacetoxy borohydride (0.455 g, 2.15 mmol) was added and stined for 15 minutes at 0°C, after which the ice bath was removed, allowed to warm to room temperature and stined over night. Additional l-methyl-4-piperidone (0.178 g, 1.57 mmol), glacial acetic acid (0.094 g, 1.57 mmol), and sodium triacetoxy borohydride (0.455 g, 2.15 mmol) were added and stined at room temperature. To the reaction solution was added sodium bicarbonate (0.721 g, 8.58 mmol) in water (10 mL) and stined for 1 hour. The organic layer was evaporated under reduced pressure. Ethyl acetate was added, the layers were partitioned, and the aqueous layer was exfracted with ethyl acetate. The organic layers were combined, washed with water and brine, dried over magnesium sulfate, filtered and evaporated under reduced pressure. The compound was purified by flash chromatography on silica using dichloromethane / ethyl acetate (1:1), 10% methanol in dichloromethane, and then 20% methanol in dichloromethane to give 400 mg (61%>) of 4-chloro-5-iodo-7-[l-(l-methyl-4-piperidinyl)-4-piperidinyl]-7H-pynolo[2,3-_ ]pyrimidine

Η NMR (DMSO-d6, 400 MHz) 8.62 (s, IH), 8.16 (s, IH), 4.66-4.58 (m, IH), 3.17 (s, IH), 3.02-3.00 (d, 2H, J = 8 Hz), 2.84-2.82 (d, 2H, J = 8 Hz), 2.33-2.26 (m, 2 H), 2.17 (s, 3H), 2.08-2.04 (m. 2H), 1.95-1.85 (m, 4H), 1.91-1.88 (d, 2H, J = 12 Hz), 1.49-1.45 (m, 2H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 m, 2.1 x 50 mm; 5%-95% acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rt 3.505 min.

4-chloro-5-(4-phenoxyphenyl)-7-[l-(l-methyl-4-piperidinyl)-4-piperidinyl]-7J3-pyrroIo[2,3-_/]pyrimidine
A mixture of 4-chloro-5-iodo-7-[l-(l-methyl-4-piperidinyl)-4-piperidinyl]-7H-pyπolo[2,3-_Z]pyrimidine (0.390 g, 0.845 mmol), 4-phenoxyphenyl boronic acid (0.199 g, 0.93 mmol), tefrakis(triphenylphosphine)pallidium (0.058 g, 0.051 mmol), and sodium carbonate (0.215 g, 2.03 mmol) were heated in a mixture of ethylene glycol dimethyl ether (20 mL) and water (10 mL) at 80°C for 7 hours under a nifrogen atmosphere. Reaction solution was allowed to cool to room temperature, and the organic layer was removed under reduced pressure. Ethyl acetate was added, the layers were partitioned and the aqueous layer was extracted with ethyl acetate (250 mL). The organic layers were combined, washed with water and brine, dried over magnesium sulfate, filtered and evaporated under reduced pressure. The residue was purified by flash chromatography on silica using 10 % methanol in dichloromethane, then 20 %> methanol in dichloromethane as eluent to give 0.241 g (51 %) of 4-chloro-5-(4-phenoxyphenyl)-7-[ 1 -(1 -methyl-4-piperidinyl)-4-piperidinyl]-7H-pynolo[2,3-_/ipyrimidine
Η NMR (DMSO-d6, 400 MHz) δ 8.99 (s, IH), 7.94 (s, IH), 7.55-7.53 (d, 2H, J = 8 Hz), 7.45-7.41 (m, 2H), 7.20-7.05 (m, 5H), 4.69 (m, IH), 3.06-3.03 (m, 2H), 2.95-2.85 (m, 2H), 2.45-2.25 (m, 4H), 2.16 (s, 3H), 2.00-1.80 (m, 5H), 1.75-1.70 (m, 2H), 1.55-1.45 (m, 2H); Waters 2690 Alliance HPLC (Symmetry Shield RP18 3.5 μm, 2.1 x 50 mm; 5%>-95%> acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min)

4-amino-5-(4-phenoxyphenyl)-7-[l-(l-methyl-4-piperidinyl)-4-piperidinyl]-7_f -pyrrolo [2,3-d\ pyrimidine

A mixture of 4-chloro-5-(4-phenoxyphenyl)-7-[l-(l-methyl-4-piperidinyl)-4-piperidinyl]-7H-pynolo[2,3-_ ]pyrimidine (0.241 g, 0.48 mmol) and concenfrated ammonium hydroxide (20 mL) in dioxane (20 mL) was heated at 120°C in a pressure vessel under a nitrogen atmosphere for 20 hours. The reaction mixture was allowed to cool to room temperature, and dioxane was removed under reduced pressure. The residue was partitioned between ethyl acetate and saturated sodium chloride aqueous solution. The aqueous layer was exfracted with ethyl acetate (500 mL). The combined organic layers were washed with water, dried over magnesium sulfate, filtered and evaporated under reduced pressure. The compound was purified by flash chromatography on silica gel using 20%> methanol in dichloromethane to 35%> methanol in dichloromethane in 5% increments, yielding 0.119 g of product which was subsequently dissolved in ethylacetate. To this solution, maleic acid (0.087 g, 0.75 mmol) in ethyl acetate was added. The solid, which precipitated, was filtered under a nifrogen atmosphere, and dried under high vacuum. The product contained some impurity. Approximately 5 mL of saturated sodium bicarbonate aqueous solution and 10 mL of dichloromethane was added. The layers were separated using an Empore extraction cartridge. The remaining material was sent for preparative ΗPLC, 38 mg was received. The product (0.038g, 0.079 mmol) was dissolved in ethyl acetate. To this solution, (0.027 g, 0.23 mmol) of maleic acid in ethyl acetate was added. The product (0.063 g) formed was a trimaleate salt. lH NMR (DMSO-d6, 400 MHz) δ 8.18 (s, IH), 7.48-7.40 (m, 5H), 7.19-7.08 (m, 5H), 6.29 (s, 6H), 4.80 (m, IH), 3.70-2.90 (m, 9H), 2.67 (s, 3H), 2.40-2.20 (m, 6 H), 1.90-1.75 (m, 2H); Waters 2690 Alliance HPLC (Symmetry Shield RPlg 3.5 μm, 2.1 x 50 mm; 5%-95% acetonitrile-0.1 M ammonium acetate over 15 min, 0.5 mL/min) Rj 4.629 min.

Examples 500 and 501

Trans-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidin-4-amine tetramaleate salt

Cis-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidin-4-amine tetramaleate salt

a)
A mixture of 4-(4-chloro-5-iodo-7H-pynolo[2,3-_ ]pyrimidin-7-yl)-l- cyclohexanone (20.0 g, 53.3 mmol), 1 -methylpiperazine (6.42 g, 128 mmol) and acetic acid (7.7 g, 128 mmol) in 1,2-dichloromethane (1 L) was stined for 15 ' minutes at ambient temperature. Sodium triacetoxyborohydride (14.7 g, 69.3 mmol) was added and stirring was continued for 20 hours. Water (400 mL) was added followed by sodium bicarbonate (26.0 g, 310 mmol). The mixture was transfened to a separatory funnel and the layers separated. The aqueous layer was exfracted with dichloromethane (300 mL). The combined organic extracts were dried over magnesium sulfate, filtered and the filtrate concentrated under reduced pressure. The resulting oil was purified by flash chromatography on silica gel (275 g) using dichloromethane/methanol (9:1) as an eluent to give cis-4-chloro-5-iodo-7-[4-(4- memylpiperazino)cyclohexyl]-7H-pynolo[2,3- /]pyrimidine (10.2 g, 42%) and trans- 4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_/]pyriιnidine (5.8 g) which contained 10% ofthe cis isomer. This mixture was stined in diethyl ether (100 mL) for 1.5 hours then the solid was collected by filfration to provide pure trans-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- yrimidine (5.0 g, 20.2%>).

Cis-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-.φyrimidine: Η NMR (DMSO-d6, 400MHz) δ 8.60 (s, IH), 7.52 (s, IH), 4.84 (m, IH), 2.06-2.53 (m, 16H), 1.79 (m, 2H), 1.62 (m, 2H); RP-HPLC (Hypersil HS C18 Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 25%-100% acetonitrile over 10 min, l mL/min) !.7.3 min; MS:MH+ 460.2.
Trans-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- yrimidine: Η NMR (DMSO-d6, 400MHz) δ 8.60 (s, IH), 7.43 (s, IH), 4.70 (m, IH), 2.42-2.67 (m, 9H), 2.32 (s, 3H), 2.09-2.18 (m, 4H), 1.76-1.86 (m, 2H), 1.53-1.61 (m, 2H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 25%-100% acetonitrile over 10 min, 1 mL/miή) \_ 6.1 mm; MS.MH4" 460.2.

b) Tert-butyl N-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)benzyl]carbamate
A mixture of 4-bromobenzylamine hydrochloride (10.0 g, 45.0 mmol), di -tert-butyl dicarbonate (11.8 g, 54.0 mmol) and dusopropylethyl amine (8.7 g, 68 mmol) in tefrahydrofuran (100 mL) was heated at reflux for 1 hour. The mixture was cooled and the solvent evaporated under reduced pressure. The material was dissolved in dichloromethane (200 mL) and then washed with water, dried over magnesium sulfate, filtered and the filtrate concentrated under reduced pressure to provide an oil (14.0 g). The oil was dissolved in N, N- dimethylformamide (250 mL) then treated with diboron pinacol ester (11.4 g, 45 mmol), potassium acetate (13.2 g, 135 mmol) and [l.l'-bis(diphenylphosphino)fenocene]dichloropalladium (II) complex with dichloromethane (1 : 1) (1.1 g, 1.35 mmol). The mixture was heated at 85°C for 18 hours then cooled and the solvent removed under reduced pressure. The residue was stined with dichloromethane (250 mL) then filtered through a pad of celite. The filfrate was concentrated under reduced pressure and the residue purified by flash chromatography on silica gel using heptane/ethyl acetate (8:2) as an eluent to provide tert-butyl N-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)benzyl]carbamate (11.8 g, 78%): : !H ΝMR (DMSO-cf6, 400MHz) δ 7.63 (d, 2H), 7.40 (t, IH), 7.23 (d, 2H), 4.14 (d, 2H), 1.39 (s, 9H), 1.28 (s, 12H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 25%>-100%> acetonitrile over 10 min, 1 mL/min) 1. 12.65 min.

c) Trans-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidin-4-amine tetramaleate salt PH 4011342C
A mixture of frans-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-./]pyrimidine (1.0 g, 2.18 mmol), tert-butyl N-[4-(4,4,5,5- tetramethyl-l,3,2-dioxaborolan-2-yl)benzyl]carbamate (0.8 g, 2.40 mmol), sodium carbonate (0.56 g, 5.24 mmol) and tefrakis(triphenyl-phosphine)palladium (70 mg, 0.131 mmol) in ethylene glycol dimethyl ether (16 mL) and water (8 mL) was heated at 85°C under an atmosphere of nitrogen for 18 hours. The mixture was cooled to ambient temperature and the solvent evaporated under reduced pressure. Water (25 mL) was added and the mixture was extracted with ethyl acetate (2 X 25 mL) and dichloromethane (25 mL). The combined organic exfracts were washed ' with brine (25 mL), dried over magnesium sulfate then fitered and the filtrate concentrated under reduced pressure. The residue was purified by flash
chromatography on silica using dichloromethane/methanol (85:15) as an eluent to yield an oil (1.0 g) which was dissolved in 1,4-dioxane (75 mL) and 30%) aqueous ammomum hydroxide (75 mL). The mixture was heated at 120°C in a sealed vessel for 18 hours then cooled to ambient temperaxue and concenfrated under reduced pressure. The residue was dissolved in acetone (50 mL) and 6Ν aqueous
hydrochloric acid (25 mL). The solution was stined at ambient temperature for 20 hours then concentrated and purified by preparative reverse-phase chromatography. Lyophilization provided a hydroscopic solid (750 mg). The solid (75 mg, 0.179 mmol) was dissolved in ethanol (1 mL) then freated with a solution of maleic acid (104 mg, 0.894 mmol) in ethanol (2 mL). The suspension which resulted was heated at 85°C with stirring for 30 minutes then cooled to ambient temperatue and the solid collected by filtration to provide frans-5-[4-(aminomethyl)phenyl]-7-[4-(4- methylpiperazino)cyclohexyl]-7H-pynolo[2,3-^pyrinnά^-4-amine tetramaleate salt as a white solid: Η NMR (DMSO- 6, 400MΗz) δ 8.19 (s, IH), 8.15 (bs, 2H), 7.54 (m, 4H), 7.49 (s, IH), 6.30 (bs, 2H), 6.14 (s, 8H), 4.60 (m, IH), 4.09 (s, 2H), 2.5-3.5 (m, 8H), 2.69 (s, 3H), 1.91-2.00 (m, 7H), 1.54-1.59 (m, 2H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 2%-25% acetonitrile over 25 min, 1 mL/min) t.6.7 min.; MS:Mir 420.2.
A similar procedure using cis-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidine was used to prepare cis-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine tetramaleate salt: Η NMR (DMSO-- 400MHz) δ 8.19 (s, IH), 8.14 (bs, 2H), 7.53 (m, 4H), 7.38 (s, IH), 6.13 (s, 8H), 4.72 (m, IH), 4.10 (s, 2H), 3.1-3.6 (m, 8H), 2.77 (s, 3H), 1.65-2.50 (m, 9H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) 1. 10.18 min.; MS.MH4" 420.2.

Examples 502 and 503
Trans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7_9-pyrrolo[2,3-< Jpyrimidin-5-yl}benzyl)benzamide
and Cis-Nl-(4-{4-amino-7-[4-(4-methyIpiperazino)cycIohexyl]-7J3-pyrrolo[2,3-._]pyrimidin-5-yl}benzyl)benzamide
A mixture of frans-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (50 mg, 0.119 mmol) in pyridine (2 mL) and dichloromethane (2 mL) was cooled to 0°C then freated with benzoyl chloride (19 mg, 0.13 mmol). The mixture was allowed to warm to ambient temperature and stined for 1 hour. The solvents were removed under reduced pressure then the residue was purified by preparative reverse phase chromatography to give frans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_/]pyrimidin-5-yl}benzyl)benzamide (50 mg) as a white solid: Η ΝMR (DMSO-./6, 400MΗz) δ 9.08 (t, IH), 8.13 (s, IH), 7.91 (m, 2H), 7.4-7.54 (m, 8H), 6.0 (bs, 2H), 4.53 (m, 3H), 2.30-2.40 (m, 5H), 2.15 (s, 3H), 1.85-1.89 (m, 10H), 1.43-1.46 (m, 2H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) t- 18.20 min.; MS:MH+ 524.3.
The cis isomer was prepared in a similar manner as described above: Η NMR (DMSO-_/6, 400MHz) δ 9.08 (t, IH), 8.14 (s, IH), 7.91 (d, 2H), 7.41-7.56 (m, IH), 7.26 (s, IH), 4.67 (m, IH), 4.54 (d, 2H), 1.5-2.5 (m, 20 H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) L 18.40 min.; MS:MH+ 524.3.

Example 504
Cis-Nl-(4-{4-amino-7-[4-(4-,ethylpiperazino)cyclohexyl]-7_3-pyrrolo[2,3-rf]pyrimidin-5-yl)benzyl)acetamide
The title compound was prepared in a similar manner cis-Nl -(4- {4-amino-7-[4-(4-mexhylpiρerazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}benzyl)benzamide :
Η ΝMR (DMSO- 6, 400MΗZ) δ 8.37 (t, 1Η), 8.14 (s, 1Η), 7.44 (d, 2Η), 7.35 (d, 2H), 7.25 (s, IH), 6.0 (bs, 2H), 4.68 (m, IH), 4.30 (d, 2H), 1.5-2.45 (m, 23 H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) tr 13.38 min.; MS.MH4" 462.3.

Example 505
Cis-5-{4-[(benzylamino)methyl]phenyl}-7-[4-(4-methylpiperazino)cyclohexyl]-7_H-pyrrolo[2,3-rf]pyrimidin-4-amine
A mixture of cis-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (100 mg, 0.238 mmol), benzaldehyde (28 mg, 0.262 mmol) and acetic acid (43 mg, 0.714 mmol) in 1,2 dichloroethane (4 mL) was stined at ambient temperature for 1 hour. Sodium triacetoxyborohydride (100 mg, 2.6 mmol) was added and the mixture was stirring continued for 2 hours. Sodium borohydride (100 mg, 2.60 mmol) was added and stirring was continued for an additional 30 minutes, the solvents were removed under reduced pressure and the residue purified by preparative reverse phase chromatography to yield cis-5-{4-[(benzylamino)methyl]phenyl}-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-_^pyrimidin-4-amine: lH NMR (DMSO-d6, 400MΗz) δ 8.13 (s, IH), 7.2-7.50 (m, 10H), 6.03 (bs, 2H), 4.67 (m, IH), 3.71 (d, 2H), 1.52-2.50 (m, 20H); RP-HPLC (Hypersil HS C18, 5 μm, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) 1. 14.42 min.; MS:MH+ 510.3.

Examples 506 and 507
Trans-benzyl N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7J_T-pyrrolo[2,3-rf]pyrimidin-5-yl}benzyl)carbamate and Cis-benzyl N-(4-{4-amino- 7-[4-(4-methylpiperazino)cyclohexyl]-7_9-pyrrolo[2,3-rf]pyrimidin-5-yl} benzyl)carb amate
A mixture of frans-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylρiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (50 mg, 0.119 mol) in pyridine (2 mL) and dichloromethane (2 mL) was cooled to 0°C then freated with benzyl chloroformate (22 mg, 0.131 mmol). The mixture was warmed to ambient temperature and stined an additional 2 hours, evaporated and the residue purified by preparative reverse-phase chromatography to give trans-benzyl Ν-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}benzyl)carbamate: Η NMR (DMSO-d6, 400MHz) 8.13 (s, IH), 7.85 (t, IH), 7.3-7.42 (m, 10 H), 6.02 (bs, 2H), 5.06 (s, 2H), 4.54 (m, IH), 4.26 (d, 2H), 1.44-2.37 (m, 20H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) t.22.15min.; MS:MH+ 554.3.
Cis-benzyl N-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}benzyl)carbamate: 'H NMR (DMSO-d6, 400MHz) 8.14 (s, IH), 7.86 (t, IH), 7.26-7.48 (m, 10 H), 6.04 (bs, 2H), 5.10 (s, 2H), 4.67 (m, IH), 4.26 (d, 2H), 1.44-1.56-2.5 (m, 20H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 5%>-50% acetonitrile over 25 min, 1 mL/min) 22.72min.; MS:MH+ 554.3.

Examples 508 and 509
Trans-N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyιfrnidin-5-yl}benzyl)-l-benzenesuIfonaιnide and Cis-N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}benzyl)-l-benzenesulfonamide

The compound was prepared from frans-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine and benzene sulfonyl chloride in a manner similar as that described for the synthesis of Trans-N 1-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}benzyl)benzamide: Η NMR (DMSO-d6, 400MHz) 8.13 (s, IH), 7.79 (d, 2H), 7.54.7.64 (m, 3H), 7.38 (s, IH), 7.28-7.35 (m, 4H), 6.0 (bs, 2H), 4.54 (m, IH), 4.06 (s, 2H), 2.3-2.5 (m, 6H), 2.14 (s, 3H), 1.86-1.94 (m, 9H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) t. 19.9 min.; MS:MH+ 560.3.
Cis-N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}benzyl)-l-benzenesulfonamide: *H NMR (DMSO-d6, 400MHz) 8.22 (s, IH), 8.14 (s, IH), 7.78 (d, 2H), 7.54-7.62 (m, 3H), 7.23-7.37 (m, 5H), 5.98 (bs, 2H), 4.67 (m, IH), 4.07 (d, 2H), 2.05-2.55 (m, 16H), 1.53-1.77 (m, 4H)); RP-HPLC (Hypersil HS Cl 8, 5 m, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) tr 20.45 min.; MS:MH+ 560.3.

Examples 510 and 511
Trans-N-(4-{4-amino-7-[4-(4-met ylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}benzyl)-N-phenylurea and Cis-N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}benzyl)-N-phenylurea
The frans-5-[4-(aminomethyl)phenyl]-7-[4-(4-methylpiperazino)cyclohexyl]- 7H-pynolo[2,3-d]pyrimidin-4-amine (50 mg, 0.119 mmol) was dissolved in dichloromethane (2 mL) then freated with phenyl isocyanate (16 mg, 0.131 mmol) and stined for 1 hour at ambient temperature. The solvent was removed under reduced pressure and the residue purified by preparative reverse phase chromatography to give frans-N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]ρyrimidin-5-yl}benzyl)-N-phenylurea: Η NMR (DMSO-d6, 400MHz) 8.67 (s, IH), 8.13 (s, IH), 7.38-7.44 (m, 7H), 7.22 (t, 2H), 6.91 (t, IH), 6.76 (t, IH), 6.02 (bs, 2H), 4.54 (m, IH), 4.35 (d, 2H), 2.37-2.50 (m, 5H), 2.14 (s, 3H), 1.86-1.95 (m, 10H), 1.40-1.49 (m, 2H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) t_ 18.8 min.; MS:MH+ 539.3.
Cis-N-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]ρyrimidin-5-yl}benzyl)-N-ρhenylurea: Η NMR (DMSO-d6, 400MHz) 8.67 (s, IH), 8.14 (s, IH), 7.40-7.47 (m, 6H), 7.20-7.24 (m, 3H), 6.89 (t, IH), 6.71 (t, IH), 6.0 (bs, 2H), 4.67 (m, IH), 4.35 (d, 2H), 2.05-2.5 (m, 16H), 1.53-1.71 (m, 4H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 5%-50% acetonitrile over 25 min, 1 mL/min) t. 19.3min.; MS:MH+ 539.3.

Examples 512 and 513
Cis-Nl-phenyl-2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2.3-d]pyrimidin-5-yl}phenyl)acetan_ide trismaleate and Trans-Nl-phenyl-2- (4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyπolo [2.3 -d]pyrimidin-5-yl}phenyl)acetamide trismaleate

a) Nl-phenyl -2-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl]acetamide

A mixture of 4-bromophenyl acetic acid (5.0 g, 23.3 mmol), and oxalyl chloride (3.25 g, 25.6 mmol) in dichloromethane (100 mL), was stined at ambient temperature, freated with 3 drops of N,N-dimethylformamide, then stined for 2.5 hours. The solvents were removed under reduced pressure then the residue was dissolved in dichloromethane (100 mL) and triethylamine (5.9 g, 58.3 mmol).
Aniline (2.4 g, 25.6 mmol) was added and the mixture was stined an additional 1 hour. The solution was washed with IN aqueous hydrochloric acid (2 X 50 mL), saturated sodium bicarbonate (100 mL), brine (50 mL), then dried over magnesium sulfate, filtered and the filfrate evaporated under reduced pressure to give Nl-phenyl-2-(4-bromophenyl)acetamide (4.62g, 68%) [RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 25%-00% acetonitrile over 10 min, 1 mL/min) 1. 10.85 min.] which was used without further purification. A mixture of Nl-phenyl-2-(4- bromophenyl)acetamide (4.62g, 15.9 mmol), diboron pinacol ester (4.45g, 17.5 mmol), potassium acetate (4.68 g, 47.8 mmol) and [1.1'- bis(diphenylphosphino)fenocene]dichloropalladium (II) complex with
dichloromethane (1:1) (0.39 g, 0.5 mmol) in N,N-dimethylformamide (90 mL) was heated at 100°C under an atmosphere of nitrogen for 18 hours then cooled to ambient temperature. The solvent was removed under reduced pressure and the residue was triturated with dichloromethane (100 mL). The insoluble material was removed by filfration through a pad of celite and then the filfrate was concenfrated under reduced pressure and purified by flash chromatography on silica gel using
dichloromethane/ethyl acetate (95:5) as an eluent to provide Nl -phenyl -2-[4- (4,4,5,5-teframethyl-l,3,2-dioxaborolan-2-yl)phenyl]acetamide (4.82 g, 89%)):^ NMR (DMSO-d6, 400MHz) 10.15 (s, IH), 7.64 (d, 2H), 2.59 (d, 2H), 7.36 (d, 2H), 7.29 (t, 2H), 7.03 (t, IH), 3.67 (s, 2H), 1.29 (s, 12H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 5%>-50%> acetonitrile over 25 min, 1 mL/min) 1. 11.7 min.

• b) Nl -phenyl-2-(4- {4-chloro-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidine-5-yl}phenyl)acetamide
A mixture of cis-4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidine (1.0 g, 2.18 mmol), Nl-phenyl -2-[4-(4,4,5,5-teframethyl- l,3,2-dioxaborolan-2-yl)phenyl]acetamide (0.81 g, 2.40 mmol), tetrakis(triphenyl- phosphine)palladium (70 mg, 0.131 mmol) and sodium carbonate monohydrate (0.65g, 2.40 mmol) in ethylene glycol dimethyl ether (16 mL) and water (8 mL) was heated at 85°C under an atmosphere of nitrogen for 18 hours. The mixture was cooled to ambient temperature and the solvent was removed under reduced pressure. The residue was purified by flash chromatography on silica using
dichloromethane/methanol (9:1) as an eluent to give Nl-phenyl-2-(4-{4-chloro-7-[4- (4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidine-5- yl}phenyl)acetamide (0.74g, 62%): Η NMR (DMSO-d6, 400MHz) 10.20 (s, IH), 8.66 (s, IH), 7.81 (s, IH), 7.62 (d, 2H), 7.52 (d, 2H), 7.41 (d, 2H), 7.31 (t, 2H), 7.04 (t, IH), 4.83 (m, IH), 3.70 (s, 2H)< 2.05-2.5 (m, 13H), 2.17 (s, 3H), 1.59-1.77 (m, 4H); RP-HPLC (Hypersil HS C18, 5 m, 100A, 250 X 4.6 mm; 25%-100%
acetonitrile over 10 min, 1 mL/min) tr 8.23 min.; MS:MH+ 543.2.

c) N-phenyl-2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidin-5-yl}phenyl)acetamide trismaleate
The Nl -phenyl-2-(4- {4-chloro-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidine-5-yl}phenyl)acetamide (0.74 g, 1.41 mmol) in 1,4-dioxane (50 mL) and concentrated ammonium hydroxide (50 mL) was heated in a pressure vessel at 120°C for 18 hours. The mixture was cooled to ambient temperature and the solvents evaporated under reduced pressure. The residue was purified by reverse-phase preparative chromatography then lyophilized to give a light yellow solid (485 mg, 68%). The material was dissolved in ethyl acetate (25 mL) and ethanol (5 mL) then treated with a hot solution of maleic acid (325 mg, 2.79 mmol) in ethyl acetate (20 mL). The slurry was cooled and the solid collected by filfration to give the cis-N-phenyl-2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidin-5-yl}ρhenyl)acetamide trismaleate as a white solid (630

' mg, 80%): Η NMR (DMSO-d6, 400MHz) 10.18 (s, IH), 8.21 (s, IH), 7.61 (d, 2H),

7.28-7.47 (m, 7H), 7.05 (dd, IH), 6.34 (bs, 2H), 6.16 (s, 6H), 4.74 (m, IH), 3.70 (s, 2H), 1.6-3.6 (20H); RP-HPLC (Hypersil HS Cl 8, 5 m, 100A, 250 X 4.6 mm; 25%- 100% acetonitrile over 10 min, 1 mL/min) t.6.33 min.; MS:MH+ 524.2.
frans-N-phenyl-2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H- pynolo[2,3-d]pyrimidin-5-yl}phenyl)acetamide trismaleate was prepared from trans- 4-chloro-5-iodo-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidine (1.0 g, 2.18 mmol) and Nl-phenyl -2-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)phenyl] acetamide in a similar method to that described for the cis-isomer: 'H NMR (DMSO-d6, 400MHz) 10.18 (s, IH), 8.18 (s, IH), 7.61 (d, 2H), 7.51 (m, 5H), 7.31 (t, 2H), 7.04 (t, IH), 6.3 (bs, 2H), 6.11 (s, 6H), 4.58 (m, IH), 3.69 (s, 2H), 2.6- 3.6 (bm, 9H), 2.68 (s, 3H), 1.91-2.04 (m, 6H), 1.51-1.59 (m, 2H); ); RP-HPLC (Hypersil HS C 18, 5 m, 1 OOA, 250 X 4.6 mm; 25%- 100% acetonitrile over 10 min, 1 mL/min) t.6.07min.; MS:MH+ 524.3.

General procedure of the preparation of urea variants.
The appropriately substituted isocyanate (1.2 equiv., 0.142 mmol) was added to a solution ofthe conesponding trans- or cis- 5-(4-amino-3-fluorophenyl)-7- [4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine (50 mg, 0.118mmol) in DMF (2.5 ml) at room temperature and the solution was shaken for 2.5 days. Water (1 ml) was added to each reaction and then concentrated in vacuo. The residues were then purified by mass actuated preparative RP-HPLC
(Micromass/Gilson, Hypersil BDS C18, 5μm, lOOx 21.2mm; 100-100% ammonium acetate (0.05 M, pH 4.5)-acetonitrile over 12.5 min, 25 mL/min) to afford products including those detailed in Table 1..
Each sample was analysed by LCMS and in each case the target ion was observed. The retention time is given in Table 1. The LCMS conditions used for the analysis of examples 1 -20, 22, 25 and 26 are:
LC Column : Hypersil BDS C18, 5μm, (lOOx 2.1mm)
Mobile Phase: 0.1M NH4OAc (pH 4.55) : MeCN
10% to 100% MeCN gradient over 8 min.
100% MeCN for 1 min.
100%) to 10% MeCN over 2 min. at 1 ml/min throughout.
MS method : APCI observing both +ve and ~ve ions.

Table 1. Urea variants

Example 532. cis-N-(4-{4-Amino-7-[4-(4- 5.41 635.3 methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-N'-(2-phenoxyphenyl)urea
Example 533. cis-N-(4-{4-Amino-7-[4-(4- 3.50 603.4 methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-N'-(2,5-dimethoxyphenyl)urea

Example 534
Nl-4-[4-amino-7-(4-piperidyI)-7H-pyrroIo[2,3-d]pyrimidin-5-yI]-2-fluorophenyl-2,3-dichloro-l-benzenesulfonamide
A solution containing Nl-4-[4-amino-7-(l-benzyl-4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-2,3-dichloro-l-benzenesulfonamide (0.156 g, 0.25 mmol) and α-chloroethylchloroformate (4 equiv., 1.0 mmol, 0.108 ml) and 1,2-dichloroethane (20 ml) was heated at reflux for a total of 24 h. The solvent was removed, methanol (10 ml) added and the reaction was heated at reflux for a further 24 h. The methanol was removed in vacuo and the residue was purified by column chromatography using neat ethyl acetate (100 ml) followed by 79:19:2 ethyl acetate: methanol: aq NH4OH as the eluent to afford Nl-4-[4-amino-7-(4-piperidyl)-7H-pynolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-2,3-dichloro-l-benzenesulfonamide (0.105 g, 82 %), RP-HPLC RT=2.64 min and m/z 534.9.

Example 535
Nl-(4-{4-amino-7-(4-oxo-cycIohexyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3-dichloro-l-benzenesulfonamide

tert-Butyl JV-4- [4-chloro-7-(l ,4-dioxaspiro [4.5] dec-8-yl)-7_ff-pyrrolo [2,3-<flpyrimidin-5-yl]-2-chlorophenyIcarbamate
A suspension ofthe 4-chloro-7-(l ,4-dioxaspiro[4.5] dec-8-yl)-5-iodo-7H-pyrrolo[2,3-d]pyrimidine (6.8 g, 16.2 mmol), tert-butyl N-[2-chloro-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl]carbamate (1.5 equiv, 8.59 g, 24.3 mmol), sodium carbonate (2.5 equiv, 4.30 g, 40.5 mmol) and Pd(PPh3)4 (4 mol%, 0.76 g, 0.65 mmol) in DME (280 ml) and degassed H2O (50 mL) was heated at 80°C under nifrogen for 16 h. The reaction was concenfrated under reduced pressure and the residue dissolved in CH2C12 (500 mL) and washed with water (300 ml). The aqueous layer was re-extracted with CH2C12 (3 x 300 mL) and the coimbined organic exfracts were combined, dried (MgSO4) and concenfrated in vacuo. Column chromatography purification over silica gel using 1 : 1 EtOAc :heptane afforded tert-butyl N-4-[4-chloro- 7-(l, 4-dioxaspiro[4.5]dec-8-yl)-7H-pyrrolo[2, 3-d]pyrimidin-5-ylJ-2-cblorophenylcarbamate as yellow oil (5.38 g), RP-HPLC RT=4.51 min and m/z 519.2 (MH").

5-(4-Amino-3-chlorophenyl)-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7JΪ-pyrrolo[2,3-</]pyrimidin-4-amine
A cloudy mixture of tert-butyl N-4-[4-chloro-7 -(1,4-dioxaspiro [4.5] dec-8-yl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl]-2-chlorophenylcarbamate ( 5.38 g, 10.35 mmol), aq. ammomum hydroxide (28 - 30 %, 100 mL) and dioxane (100 mL) was placed in a , sealed vessel at ambient temperature then heated to 120°C with stirring for 24 h. The reaction was concenfrated in vacuo, diluted with EtOAc (300 mL), washed with brine (2 x 150 mL), dried (MgSO4) and concenfrated under reduced pressure and scrupulously dried to afford 5-(4-amino-3-chlorophenyl)-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine as a brown solid (4.0 g), RP-HPLC (5 to 85 % CH3CN in 0.1 N aqueous ammonium acetate over 30 min at 1 mL/min using a Waters Deltapak C 18, 30θA, 150 x 3.9 mm column) RT= 14.049 min., 72%.

4-[4-ammo-5-(4-amino-3-chIorophenyI)-7_?J-pyrrolo[2,3-< |pyrimidin-7-yl]-l-cyclohexanone
5 HC1 (700 mL) was added slowly to a solution of 5-(4-amino-3-chlorophenyl)-7-(l,4-dioxaspiro[4.5]dec-8-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-amme (4.0g, 10.0 mmol) in acetone (300 mL) at 0°C then allowed to warm to ambient temperature for 24 h. The acetone was removed under reduced pressure and the acidic layer was basified to approx. pH 8 using sat. aq. Na2CO3. After extraction with CH2C12 (4 x 300 mL), the combined organic layers were dried (MgSO4) and concentrated to afford 4-[4-amino-5-(4-amino-3-chlorophenyl)-7H-pyrrolo[2,3-d]pyrimidin-7-yl]-l-cyclohexanone as abiege solid (1.71 g), RP-HPLC (5 to 85 %> CH3CN in 0.1 N aqueous ammonium acetate over 30 min at 1 mL/min using a Waters Deltapak C18, 300A, 150 x 3.9 mm column) RT= 12.392 min., 75%.

Nl-(4-{4-amino-7-(4-oxo-cycIohexyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2- fluorophenyl)-2,3-dichloro-l-benzenesulfonamide
2,3-Dichlorobenzenesulfonyl chloride (1.1 equiv., 1.04 g, 4.26 mmol) was added to a solution of 4-[4-amino-5-(4-amino-3-chlorophenyl)- 7H-pyrrolo[2, 3-dJpyrimidin-7-ylJ-l -cyclohexanone (1.38 g, 3.87 mmol) in pyridine (25 mL) at 40°C. After 3 h. at 40°C and 14 h at ambient temperature, additional 2,3-dichlorobenzenesulfonyl chloride (1.2 g) was added and the reaction stined at 40°C for a further 24 h. The reaction was concenfrated in vacuo, dissolved in CH2C12 (500 ml) and washed with sat. aq.
(2 x 100 ml). The organic layer was dried (MgSO4) and
concentrated in vacuo to afford a brown solid. Purification by column
chromatography over silica gel using 1% MeOH in dichloromethane as the eluent afforded as a white solid (60% pure). Trituration with hot ethyl acetate gave Nl-(4-{4-amino-7-(4-oxo-cyclohexyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3-dichloro-l-benzenesulfonamide as a white solid (70 mg), ), RP-HPLC RT=3.14 min and m/z 564.2 (MH+).

Example 536
trans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3-dichloro-l-benzenesulfonamide
To a solution ofNl-(4-{4-amino-7-(4-oxo-cyclohexyl)-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3-dichloro-l-benzenesulfonamide (0.2 g, 0.35 mmol), N-methylpiperazine (3 equiv, 1.05 mmol, 0.116 mL) and glacial acetic acid (3 equiv., 0.061 mL, 1.05 mmol) in 1,2-dichloroethane (20 mL) under nifrogen was added sodium triacetoxyborohydride (1.3 equiv., 0.096 g, 0.455 mmol). The solution was stined for 18 hr then additional sodium triacetoxyborohydride (0.10 g) was added and the reaction continued for a further 48 h. The reaction was concentrated in vacuo, partitioned between dichloromethane (100 mL) and sat. aq. NaHCO3 (100 mL). The aqueous layer was re-extracted with dichloromethane (4 x 100 mL) and the combined organic layers were dried over magnesium sulfate and evaporated to dryness. Purification by column chromatography over silica gel using
dichloromethane:methanol (19 : 1) as the eluent afforded frans-Nl-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-2-fluorophenyl)-2,3 -dichloro- 1 -benzenesulfonamide
as a white solid (10 mg), RP-HPLC (5 to 85 %> CH3CN in 0.1 N aqueous ammonium acetate over 30 min at 1 mL/min using a Waters Deltapak C18, 30θA, 150 x 3.9 mm column) RT= 14.475 min., 100% and m/z 648.1 (MIT)

Example 537
1. 4-chloro-5-iodo-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pyrrolo[2,3- d]pyrimidme

4-chloro-5-iodo-7H-pynolo[2,3-d]pyrimidine (9.78 g, 0.035mol) was dissolved in tefrahydrofuran (400 ml) at room temperature under a nifrogen atmosphere. Tropine (10 g, 0.071 mol) and triphenylphosphine (18.6 g, 0.071 mol) were added to this solution. The reaction mixture was cooled to 0° C and diethyl azodicarboxylate (12.35 g, 0.071 mol) was added slowly. Stirring was continued at 0° C for one hour then the reaction mixture was warmed to room temperature and stined for 20 hours. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in ethyl acetate (250-300 ml) and concenfrated again under reduced pressure. A precipitate formed which was collected by filfration and washed with ethyl acetate to give 4-chloro-5-iodo-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidine as a light yellow solid (43%, 6.09 g,0.015 mol): Η NMR (DMSO-d6, 400 MHz) δ 8.64 (s, IH), 8.13 (s, IH), 4.98 (m, IH), 3.23 (m, 2H), 2.29 (s, 3H), 2.22 (m, 2H), 2.04 (m, 2H), 1.70 (m, 4H). TLC
(dichloromethane/methanol = 9:1) Rf 0.68; LC/MS: MH+= 402.9.

2. tert-butyl N-[4-(4-chloro-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H- pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]carbamate

A suspension of 4-chloro-5-iodo-7-(8-methyl-8-aza[3.2.1 ]bicyclooctan-3-yl)-7Η-pynolo[2,3-d]pyrimidine (6.09 g, 0.015 mol), tert-butyl Ν-[2-fluoro-4-(4,4,5,5-teframethyl-l,3,2-dioxaborolan-2-yl)phenyl]carbamate (7.64 g, 0.023 mol), tetrakis(triphenylphosphine)palladium(0) (699 mg, 0.605 mmol) and sodium carbonate (4.01 g, 0.039 mol) in degassed water (45 mL) and dimethoxyethane (240 mL) was heated at 80 °C for 36 h. The reaction mixture was concentrated under reduced pressure, then diluted with dichloromethane and washed with 10%> aqueous sodium carbonate, followed by brine. The organic layer was dried over sodium sulfate and concenfrated under reduced pressure. The filfrate was purified by column chromatography on silica gel eluting with 5%> methanol / dichloromethane/2%> ammonium hydroxide to afford tert-butyl N-[4-(4-chloro-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]carbamate as a white foam (6.62 g, 0.014 mol): Η ΝMR (DMSO-d6, 400MΗz) 9.02 (hrs, IH), 8.66 (m, IH), 8.03 (s, IH), 7.65 (m, IH), 7.39 (m, IH), 7.30 (m, IH), 5.76 (s, IH), 5.05 (m, 2H), 3.26 (brs, 3H), 2.26 (m, 6H), 2.06 (m, 2H), 1.73 (m, 5H), 1.47 (s, 9H). LC/MS: MH+= 486.2.

3. 5-(4-amino-3-fluorophenyl)-7-(8-methyl-8-aza[3.2.1 ]bicyclooctan-3-yl)-7H- pynolo[2,3-d]pyrimidin-4-amine

A solution of tert-butyl N-[4-(4-chloro-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]carbamate (6.62 g, 0.014 mol) in dioxane (100 mL) and ammonium hydroxide (100 mL) was heated at 120 °C in a stainless steel bomb for 18 hours. The reaction mixture was concenfrated under reduced pressure, then diluted with ethyl acetate and washed with water. The aqueous layer was concenfrated under reduced pressure at 60 °C and the remaining solid was dried on the lyophilizer. Isolated 5-(4-amino-3-fluorophenyl)-7-(8-methyl-8-aza[3.2.1 ]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-4-amine as a brown solid (5.73 g, 0.016 mol): ); TLC (dichloromethane/methanol = 9:1) R_ 0.147. LC/MS: ML?= 367.1.

4. Nl-[4-(4-amino-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-ρynolo[2,3- d]pyrimidin-5-yl)-2-fluorophenyl]-2,3-dichloro-l-benzenesulfonamide 2,3- dichlorobenzensulfonic acid salt

A mixture of 5-(4-amino-3-fluorophenyl)-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7Η-pynolo[2,3-d]pyrimidin-4-amine (4.0 g, 0.011 mol) and 2,3-dichlorobenzenesulfonyl chloride (2.95 g, 0.012 mol) in pyridine (55 mL) was heated at 40 °C for 18 hours. Additional 2,3-dichlorobenzenesulfonyl chloride (429 mg, 1.75 mmol and 671 mg, 2.73 mmol) was added over approximately 3 days. The reaction mixture was concenfrated under reduced pressure to give an orange oil/foam (10 g, 0.017 mol). The crude material was purified twice by column chromatography on silica gel. The first column was eluted with 10% methanol / dichloromethane, followed by 10%> methanol / dichloromethane/2%) ammomum hydroxide to afford Nl-[4-(4-amino-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-2,3-dichloro-l-benzenesulfonamide as an orange oil (7.2 g, 80% purity by HPLC). The second column was eluted with a 0% to 10%o methanol/dichloromethane/2% ammomum hydroxide gradient to afford Nl-[4-(4-amino-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl] -2,3 -dichloro-1 -benzenesulfonamide as a light yellow foam (1.77 g, 3.08 mmol, 99% purity by HPLC): IH NMR (DMSO-d6, 400MHz) 8.15 (s, IH), 7.95 (d, IH), 7.86 (m, 2H), 7.58 (m, IH), 7.50 (m, IH), 7.30 (m, 3H), 7.13 (m, 2H), 6.18 (br s, 2H), 5.05 (m, IH), 3.91 (m, 2H), 3.21 (s, IH), 2.75 (s, 3H), 2.26 (m, 2H), 2.04 (m, 4H). LC/MS: MH+= 575.0.

5. Nl-[4-(4-ammo-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7ET- pyrrolo [2, 3-d] pyrimidin-5-yl)-2-flu orophenyl] -2, 3-dichloro-l - benzenesulfonamide

A solution of Nl-[4-(4-amino-7-(8-methyl-8-aza[3.2.1]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-2,3-dichloro-l-benzenesulfonamide 23 dichlorobenzensulfonic acid salt (106 mg, 0.133 mmol) in dichloromethane (30mL) and a minimal amount of methanol was washed with saturated aqueous sodium bicarbonate. The organic layer was dried over sodium sulfate then concentrated under reduced pressure to afford Nl-[4-(4-amino-7-(8-methyl-8-aza[3.2.1 ]bicyclooctan-3-yl)-7H-pynolo[2,3-d]pyrimidin-5-yl)-2-fluorophenyl]-2,3-dichloro-1 -benzenesulfonamide as an off-white solid (74 mg, 0.129 mmol): IH NMR (DMSO-d6, 400MHz) δ 8.13 (s, IH), 7.99 (d, IH), 7.77 (d, IH), 7.44 (t, IH), 7.28 (t, IH), 7.06 (m, 3H), 6.12 (br s, 2H), 5.01 (m, IH), 3.69 (br s, 2H), 2.62 (s, 3H), 2.35 (m, 2H), 2.21 (m, 2H), 1.94 (m, 4H). LC/MS: MH+= 575.1..

Example 538
Nl-4-(4-amino-7-{4-[4-(l-methylpiperidyl)piperidyl]-7H-pyrrolo[2,3-d]pyrimidin-5-yl])-2-fluorophenyl-4-fluoro-l-benzenesulfonamide
To a solution containing Nl-4-[4-amino-7-(4-piperidyl)-7H-pyrrolo[2,3-d]pyrimidin-5-yl]-2-fluorophenyl-4-fluoro-l-benzenesulfonamide (50 mg, 0.103 mmol), l-methylpiperid-4-one (1.5 equiv., 17.5 mg, 0.019 ml) and glacial acetic acid (0.025 ml) in N-methyl-2-pynolidinone (3 ml) was added sodium
triacetoxyborohydride (1.3 equiv., 28.1 mg). The reaction was stined for 24 h at room temperature prior to the addition of exfra sodium triacetoxyborohydride (1.3 equiv., 28.1 mg) was added and the reaction continued for a further 48 h. The reaction was concentrated in vacuo, partitioned between dichloromethane (100 mL) and sat. aq. NaHCO3 (100 mL). The aqueous layer was re-extracted with
dichloromethane (4 x 100 mL) and the combined organic layers were dried over magnesium sulfate and evaporated to dryness. Purification by column
chromatography over silica gel using dichloromethane:methanol: aqueous ammonium hydroxide (78:19:3) as the eluent afforded a biege solid (45 mg, 75 %>), RP-HPLC RT=2.28 min and m/z 582.1.

General Procedure for Substituted Pyrrolopyrimidine Aryl sulfonamides
A 0.19 M solution of 5-(4-amino-3-fluorophenyl)-7-[4-(4- methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-4-amine in pyridine was added one equivalent of substituted aryl sulfonyl chloride. The mixture was heated to 45o C while being shaken in an Incubator Shaker for 24h. The reaction mixture was purified by using mass actuated preparative RP-HPLC
(Micromass/Gilson, Hypersil BDS C18, 5u, 100x21.2mm; 100-100%
ammonium acetate (0.05 M, pH 4.5)-acetonitrile over 12.5 min, 25 mL/min).

Structure RT/min Example

LC, rt = 3.08 MH+ = 618.3 539

LC, rt = 3.29 MH+ = 758.1 540
-

«_>« λ LC, rt = 2.41 MH+ = 582.2 541
$

LC, rt = 2.76 MH+ = 632.2 542

LC, rt = 2.57 MH+ = 632.1 543

LC, rt = 2.49 MH+ = 623.2 544


LC, rt = 2.38 MH+ = 606.3 ' 546
t

The following compounds are prepared using the general methods described above.

rraw5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-benzoxazol-2-amine
Erα7._.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-methylbenzoxazol)-2-amine
rra«-J-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(5 -methylbenzoxazol)-2-amine
rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(6-methylbenzoxazol)-2-amine
_rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-methylbenzoxazol)-2-amine
rr rø-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,5-dimethylbenzoxazol)-2-amine
rra«5,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4,6-dimethylbenzoxazol)-2-amine
Era?.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo 2,3-d]pyrimidin-5-yl} phenyl)- 1 ,3-(4,7-dimethylbenzoxazol)-2-amine
Jr n-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5,6-dimethylbenzoxazol)-2-amine
_rran5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,7-dimethylbenzoxazol)-2-amine
_ rΩ7.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-dimethylbenzoxazol)-2-amine
Er_.«5'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluorobenzoxazol)-2-amine
Er_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(5 -fluorobenzoxazol)-2-amine

2r n^-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-fluorobenzoxazol)-2-amine
rr_.«5'-N2-(4-{4-anιino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluorobenzoxazol)-2-amine Trans-~N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3-(4,5-difluorobenzoxazol)-2-amine
Trans-~N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- l,3-(4,6-difluorobenzoxazol)-2-amine
Tra ns-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- l,3-(4,7-difluorobenzoxazol)-2-amine
Trans-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- l,3-(5,6-difluorolbenzoxazol)-2-amine

Trans-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin- 5 -yl} pheny 1)- l,3-(5,7-difluorobenzoxazol)-2-amine

Eran,!. -N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3 -(6,7-difluorobenzoxazol)-2-amine
Trans-~N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} pheny 1)- 1 ,3-(4-chlorobenzoxazol)-2-amine
Trans-~N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5-chlorobenzoxazol)-2-amine

Trans-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-chlorobenzoxazol)-2-amine

Trans-Η2-(4- {4-amino-7- 4-(4-methylρiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-chlorobenzoxazol)-2-amine

Trans-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- l,3-(4,5-dichlorobenzoxazol)-2-amine

Trans~~N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- l,3-(4,6-dichlorobenzoxazol)-2-amine

Trans-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) 1 ,3-(4,7-dichlorobeι__zoxazol)-2-amine

Trans-N2-(4- {4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl) 1 ,3-(5,6-dichlorolbenzoxazol)-2-amine
rrα«5-N2-(4-{4-amino-7- 4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,7-dichlorobenzoxazol)-2-amine rra ._?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyιinidin-5-yl}phenyl)-l,3-(6,7-dichlorobenzoxazol)-2-amine
rr_ZR^-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-4-methylbenzoxazol)-2-amine rrα«5-N2-(4-{4-_tmino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyιfrmdin-5-yl}phenyl)-l,3-(5-fluoro-4-methylberιzoxazol)-2-amine rrαn5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-4-methylbenzoxazol)-2-amine

ErαR_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-fluoro-4-methylbenzoxazol)-2-amine rrΩ7.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-4-methylbenzoxazol)-2-amine rr_.n_r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-4-methylbenzoxazol)-2-amine

_Tr_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chloro-5-methylber_zoxazol)-2-amine rrαw_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-5-methylbenzoxazol)-2-amine

Erαπ5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-5-methylbenzoxazol)-2-amine

Erαnj-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-fluoro-5-methylbenzoxazol)-2-amine

_Tr_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-5-methylbeι__zoxazol)-2-amine rr_.τ.5'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-5-methylbeι__zoxazol)-2-amine rrα/._.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrirmdin-5-yl}phenyl)-l,3-(4-chloro-6-methylbenzoxazol)-2-amine rr_.«5-N2-(4-{4-__n_ino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-6-methylbenzoxazol)-2-amine rr «5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-6-methylbenzoxazol)-2-amine

rra»5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5-fluoro-6-methylbenzoxazol)-2-amine

Eraπ-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrirnidin-5-yl}phenyl)-l,3-(7-chloro-6-methylbeι__zoxazol)-2-amine

_Trα/.-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-6-methylbenzoxazol)-2-amine

_Tr R_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chloro-7-methylbenzoxazol)-2-amine

rrαn5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-7-methylbenzoxazol)-2-amine rr_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5-chloro-7-methylbenzoxazol)-2-amine

Erα/.>s,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo '2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluoro-7-methylbenzoxazol)-2-amine

_Trα/.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-7-methylbenzoxazol)-2-amine

Trans-N2-(4- {4-amino-7- [4-(4-methy lpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-fluoro-7-methylbenzoxazol)-2-amine

rr-.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-benzothiazol-2-amine
rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4-methylbenzothiazol)-2-amine
_rra«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-methylbenzothiazol)-2-amine t rr_.π_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-methylbenzothiazol)-2-amine
EraR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-methylbenzothiazol)-2-amine rrαR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrinfidm-5-yl}phenyl)-l,3-(4,5-dimethylbeι_zo1hiazol)-2-amine
Trans-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)-l,3-(4,6-dimethylbenzothiazol)-2-amine
Trans -N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)- 1 ,3-(4,7-dimethylbenzothiazol)-2-amine
rra«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5,6-dimethylbenzothiazol)-2-amine
rr «5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5,7-dimethylbenzothiazol)-2-amine
Erαn-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-dimethylbenzothiazol)-2-amine
rra«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluorobenzothiazol)-2-amine
rr_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluorobenzothiazol)-2-amine
Erα?. .-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-fluorobenzothiazol)-2-amine

7>α«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-fluorobenzothiazol)-2-amine
Erαπ_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,5-difluorobenzothiazol)-2-amine
rrαR_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,6-difluorobenzothiazol)-2-amine
rran_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,7-difluorobenzothiazol)-2-amine
rrαR_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,6-difluorolbenzothiazol)-2-amine
rra«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,7-difluorobenzothiazol)-2-amine rrαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyriι dm-5-yl}phenyl)-l,3-(6,7-difluorober_zot azol)-2-amine
r_.«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyι nidin-5-yl}phenyl)-l,3-(4-chlorober_zothiazol)-2-amine
Er n5'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chlorobenzothiazol)-2-amine
rr_.n_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chlorobenzothiazol)-2-amine
Erα?z_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chlorobenzothiazol)-2-amine
Er_.«_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,5-dichlorobenzothiazol)-2-amine
rr_-π_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4,6-dichlorobenzothiazol)-2-amine
Er_zw5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,7-dichloroberιzothiazol)-2-amine

rra«_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,6-dichlorolbenzothiazol)-2-amine
Tra ns-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyπolo 2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(5 ,7-dichlorobenzothiazol)-2-amine
rr_.H5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-dichlorobenzothiazol)-2-amine
rrΩR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-4-methylbenzothiazol)-2-amine rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluoro-4-methylbenzothiazol)-2-amine rrα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-chloro-4-methylbenzothiazol)-2-amine

Trans -N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrinndm-5-yl}phenyl)-l,3-(6-fluoro-4-methylbenzotMazol)-2-aιmne r n_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-chloro-4-methylbenzothiazol)-2-amine rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-fluoro-4-methylbenzothiazol)-2-amine rr-.«5-N2-(4-{4-amino-7-[4-(4-mexhylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyιmfrdm-5-yl}phenyl)-l,3-(4-cMoro-5-methylber_zothiazol)-2-amine rαn_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4-fluoro-5-methylbenzothiazol)-2-amine rra/.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-chloro-5-methylbenzothiazol)-2-amine

Jr_z«-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-fluoro-5-methylbenzothiazol)-2-amine

rr .5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-5-methylbenzothiazol)-2-amine

rr_.«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-5-methylber_zothiazol)-2-amine rr /.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-l,3-(4-chloro-6-methylberιzotMazol)-2-amine rrαw5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-6-methylbenzothiazol)-2-amine rr rø-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5-chloro-6-methylbenzothiazol)-2-amine

Eran_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5-fluoro-6-methylbenzothiazol)-2-amine

7rα77_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-6-methylbenzothiazol)-2-amine

Erα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-fluoro-6-methylbenzothiazol)-2-amine Erαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyr_nιidin-5-yl}phenyl)-l,3-(4-cl oro-7-methylber_zotl_iazol)-2-amine rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-7-methylbeι__zotMazol)-2-amine

rrβ«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-7-methylbenzothiazol)-2-amine rrαR-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluoro-7-methylbenzothiazol)-2-amine rrα?.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-cUoro-7-methylberιzothiazol)-2-amine

Tr_.n..-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-fluoro-7-methylbenzothiazol)-2-amine

7r n_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-benzimidazol-2-amine

Eraw_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]p5^imidin-5-yl}phenyl)-(4-methylbenzimidazol)-2-amine
7'ran-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyτimidin-5-yl}phenyl)-(5-methylbenzimidazol)-2-amine
rr_.R_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-methylbenzimidazol)-2-amine

rr_.τ._.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-methylbenzimidazol)-2-amine
Er_.n-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dimethylbenzimidazol)-2-amine
Er «_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dimethylbenzimidazol)-2-amine
_ rΩ7.-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dimethylbenzimidazol)-2-amine

Erα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dimethylbenzimidazol)-2-amine

7r_ -?-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5 -yl} pheny l)-(5 ,7-dimethylbenzimidazol)-2-amine rr_.«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-(6,7-dimethylbertzimidazol)-2-amine
rran_r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrinήdin-5-yl}phenyl)-(4-fluorobenzimidazol)-2-amine
rr_zw_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluorobenzimidazol)-2-amine
Era«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluorobenzimidazol)-2-amine
rrα«_.-N2-(4-{4-amino-7-[4-(4-methylρiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluorobenzimidazol)-2-amine

Er_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,5-difluorobenzimidazol)-2-amine
rrαR_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,6-difluorobenzimidazol)-2-amine
Trans-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3-d]pyrimidin-5-yl} phenyl)-(4,7-difluorobenzimidazol)-2-amine
rra«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} pheny l)-(5 ,6-difluorolbenzimidazol)-2-amine
_Tr_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,7-difluorobenzimidazol)-2-amine
rrα«-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]p5τimidin-5-yl}phenyl)-(6,7-difluorobenzimidazol)-2-amine
rr-.n-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chlorobenzimidazol)-2-amine
Erα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chlorobenzimidazol)-2-amine
Era«5-N2-(4-{4-anιino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-cWoroberιziιτιidazol)-2-amine
rr n5,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrirnidin-5-yl}phenyl)-(7-chlorobenzimidazol)-2-amine Erα«_.-N2-(4-{4-amino-7-[4-(4-mexhylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dichlorobenzimidazol)-2-amine
rα«6'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dichlorobenzimidazol)-2-amine
rra«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dichlorobenzimidazol)-2-amine
Era/.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dichlorolbenzimidazol)-2-amine

Trans-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dichlorobenzimidazol)-2-amine
rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dichlorobenzimidazol)-2-amine
r n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-4-methylbenzimidazol)-2-amine rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-4-methylbenzimidazol)-2-amine
Erαn5-N2-(4-{4-anιino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl)-(6-chloro-4-methylbenzimidazol)-2-amine
Er_z?.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-4-methylbenzimidazol)-2-amine rrατ._.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-4-methylbenzimidazol)-2-amine
Era?.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-4-methylbenzimidazol)-2-amine
Erαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-5-methylbenzimidazol)-2-amine rrαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-5-methylbenzimidazol)-2-amine
Tra ns -N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-5-methylbenzimidazol)-2-amine rr<3n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-(6-fluoro-5-methylbenzinήdazol)-2-amine rrα7._?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-5-methylbenzimidazol)-2-amine rr «-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} pheny l)-(7-fluoro-5 -methylbenzimidazol)-2-amine

Trans-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)-(4-chloro-6-methylbenzimidazol)-2-amine ran_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-6-methylbenzimidazol)-2-amine rr_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-6-methylbenzimidazol)-2-amine
Era« ,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-6-methylber_zimidazol)-2-anιine
Erα«-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-6-methylber_zimidazol)-2-amine rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-6-methylbenzimidazol)-2-amine rra?._.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-7-methylbenzimidazol)-2-amine rr_.«-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-7-methylbenzimidazol)-2-amine
_ raR-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-7-methylbenzimidazol)-2-amine rr_.M_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriιmdin-5-yl}phenyl)-(5-fluoro-7-methylber_ziιm-dazol)-2-amine rrα«_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyι nidin-5-yl}phenyl)-(6-cMoro-7-methylbenzimidazol)-2-amine
Trans-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyιimidm-5-yl}phenyl)-(6-fluoro-7-methylbeι__zimidazol)-2-amine rrαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l-methylbenzimidazol-2-amine
rrαR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethylbenzimidazol)-2-amine

Trα7.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l ,5-dimethylbenzimidazol)-2-amine
Trans-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]p5σimidin-5-yl}phenyl)-(l,6-dimethylbenzimidazol)-2-amine
Trans-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethylbenzimidazol)-2-amine
rrΩ7.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,4,5-trimethylbenzimidazol)-2-amine
rrα .5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,4,6-trimethylbenzimidazol)-2-amine
Er R5,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l ,4,7-trimethylbenzimidazol)-2-amine
7r /.-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,5,6-trimethylbenzimidazol)-2-amine
_ r_.«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,5,7-trimethylbenzimidazol)-2-amine
_rra7.-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,6,7-trimethylbenzimidazol)-2-amine
rrα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro- 1 -methylbenzimidazol)-2-amine
Trαw-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro- 1 -methylbenzimidazol)-2-amine rra«-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro- 1 -methylbenzimidazol)-2-amine
Er /-5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro- 1 -methylbenzimidazol)-2-amine rr w5-N2-(4-{4-anfrno-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,5-difluoro-l-methylberιzimidazol)-2-amine

rraR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)-(4,6-difluoro- 1 -methylbenzimidazol)-2-amine

Erα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,7-difluoro-l-methylbenzimidazol)-2-amine rrαπ_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,6-difluoro-l-methylbenzimidazol)-2-amine rαn_.-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,7-difluoro-l-methylbenzimidazol)-2-amine rrΩR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6,7-difluoro-l-methylbenzimidazol)-2-amine

_Trα«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-l-methylbenzimidazol)-2-amine
Erα«5-N2-(4-{4--Unino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l-methylbenzimidazol)-2-amine rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro- 1 -methylbenzimidazol)-2-amine
Er «-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-l-methylbenzimidazol)-2-amine
_Trα7.-j-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dichloro-l-methylbenzimidazol)-2-amine

_Tr_.n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dichloro-l-methylbenzimidazol)-2-amine

_Trα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dichlorobenzimidazol)-2-amine
rr /ϊ-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dichloro-l-methylbenzimidazol)-2-amine rrα«5-N2-(4-{4-_urιino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dichloro-l-methylbenzimidazol)-2-amine Era«5'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dichloro-l-methylbenzimidazol)-2-amine rraR_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l,4-dimethylbenzimidazol)-2-amine

Er_.7.-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethyl-5-fluorobenzimidazol)-2-amine

_ ra«_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro- 1 ,4-dimethylbenzimidazol)-2-amine Trans-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethyl-6-fluorobenzimidazol)-2-amine

Er_.«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-l,4-dimethylbenzimidazol)-2-amine

Er_z«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethyl-7-fluorobenzimidazol)-2-amine

Erα«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-l,5-dimethylbenzimidazol)-2-amine

Er «5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyriιnidin-5-yl}phenyl)-(l,5-(hmethyl-4-fluorobenzimidazol)-2-amine

Tran J-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-l,5-dimethylbenzimidazol)-2-amine

rrαR-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} pheny l)-( 1 , 5 -dimethyl-6-fluorobenzimidazol)-2-amine

_Tr «-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyriιmdin-5-yl}phenyl)-(7-cMoro-l,5-dimethylbenzimidazol)-2-amine

Erαπ5-N2-(4-{4-anιino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,5-dimethyl-7-fluorobenzimidazol)-2-amine rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-cMoro-l,6-dimethylberιzinήdazol)-2-amine

Eran5-N2-(4-{4-amino-7-[4-(4-mexhylpiperazino)cyclohexyl]-7H-pynolo 2,3- d]pyrimidin-5-yl}phenyl)-(l,6-dimethyl-4-fluorober_zimidazol)-2-amine rr_.n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l,6-dimethylbenzimidazol)-2-amine r n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyι_imidin-5-yl}phenyl)-(l,6-dimethyl-5-fluorober_zimidazol)-2-amine

Trans-'N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)-(7-chloro-l,6-dimethylbenzimidazol)-2-amine rr_zw5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3T d]pyrimidin-5-yl}phenyl)-(l,6-dimethyl-7-fluorobenzimidazol)-2-amine Trans-~N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-l,7-dimethylbenzimidazol)-2-amine rrαR_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethyl-4-fluorobenzimidazol)-2-amine rr_z7.-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l,7-dimethylbenzimidazol)-2-amine rrαn-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethyl-5-fluorobenzimidazol)-2-amine

Er R-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-l,7-dimethylbenzimidazol)-2-amine rrαn5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethyl-6-fluorobenzimidazol)-2-amine rran-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-quinazolino-2-amine
rrα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-(4-methylquinazolino)-2-amine
_ rαo-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-methylquinazolino)-2-amine

rrαn_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-methylquinazolino)-2-amine
rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidin-5-yl}phenyl)-(7-methylquinazolino)-2-amine
rra«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyι_imidin-5-yl}phenyl)-(4,5-dimethylquinazolino)-2-amine
rrα«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dimethylquinazolino)-2-amine
rα7.j-N2-(4-{4-.tmino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dimethylquinazolino)-2-amine
Eran-.-N2-(4-{4-amino-7-[4-(4-methylpiperazmo)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dimethylquinazolino)-2-amine
Trans -N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dimethylquinazolino)-2-amine
Erαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dimethylquinazolino)-2-amine
rr «5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoroquinazolino)-2-amine
rrα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoroquinazolino)-2-amine
rrαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoroquinazolino)-2-amine
rr-.R-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrrmidin-5-yl}phenyl)-(7-fluoroquinazolino)-2-amine
rrα7._--N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-difluoroquinazolino)-2-amine
7ra?._.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-difluoroquinazolino)-2-amine

rrαn,s-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-difluoroquinazolino)-2-amine
rrαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-difluorolquinazolino)-2-amine
rr__«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3- d]pyrimidin-5-yl}phenyl)-(5,7-difluoroquinazolino)-2-amine
_Tr R5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6,7-difluoroquinazolino)-2-amine
_ r «5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloroquinazolino)-2-amine
rrαn5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloroquinazolino)-2-amine
rrα«_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloroquinazolino)-2-amine
_Tra«5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloroquinazolino)-2-amine
rrα«_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dichloroquinazolino)-2-amine
JrΩ7ϊ_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dichloroquinazolino)-2-amine
rrαn_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,7-chchloroquinazolino)-2-amine
ErΩRj'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dichlorolquinazolino)-2-amine

Tra ns-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)-(5,7-dichloroquinazolino)-2-amine
rra?.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dichloroquinazolino)-2-amine

rrα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-4-methylquinazolino)-2-amine
Er_.R_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-(5-fluoro-4-methylqumazolino)-2-amine
ErαR_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-4-methylqumazolino)-2-amine rrαn5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidm-5-yl}phenyl)-(6-fluoro-4-methylqumazolino)-2-amine
rrαn5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-4-methylquinazolino)-2-amine rr_.«_.-N2-(4-{4-amino-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo[2,3-d]pyriιmdin-5-yl}phenyl)-(7-fluoro-4-methylqumazolinol)-2-amine
7>α?.-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-5-methylquinazolino)-2-amine rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)-(4-fluoro-5 -methylquinazolino)-2-amine
_Tr_.«_.-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-5-methylquinazolino)-2-amine
_Tr_.»_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-5-methylquinazolino)-2-amine rrαn_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-5-methylquinazolino)-2-amine
_Traw_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-5-methylquinazolino)-2-amine rrαR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-6-methylquinazolino)-2-amine rrΩR..-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-6-methylquinazolino)-2-amine

_ rα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-6-methylquinazolino)-2-amine rr π_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-ρynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-6-methylquinazolino)-2-amine rraR_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidm-5-yl}phenyl)-(7-cWoro-6-me1hylquinazolino)-2-arnine
7rαR-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyι__midin-5-yl}phenyl)-(7-fluoro-6-methylquinazolino)-2-amine rr_zR5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidin-5-yl}phenyl)-(4-cMoro-7-methylqumazolino)-2-amine
_ rαR_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriimdin-5-yl}phenyl)-(4-fluoro-7-methylquinazolino)-2-amine
Erα«_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-7-methylquinazolino)-2-amine rr n5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-7-methylquinazolino)-2-amine
_ ra/.5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-7-methylquinazolino)-2-amine
Trans -N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-7-methylquinazolino)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-benzoxazol-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-methylbenzoxazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-methylbenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3 -(6-methylbenzoxazol)-2-amine
Ct5,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-methylbenzoxazol)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,5-dimethylbenzoxazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,6-dimethylbenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,7-dimethylbenzoxazol)-2-amine
C-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,6-dimethylbenzoxazol)-2-amine
Ct-r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidm-5-yl}phenyl)-l,3-(5,7-dimethylbenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl}phenyl)- 1 ,3 -(6,7-dimethylbenzoxazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluorobenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluorobenzoxazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(6-fluorobenzoxazol)-2-amine
Cis-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluorobenzoxazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,5-difluorobenzoxazol)-2-amine
Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)- 1 ,3-(4,6-difluorobenzoxazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,7-difluorobenzoxazol)-2-amine

Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(5 ,6-difluorolbenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,7-difluorobenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-difluorobenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriιmdin-5-yl}phenyl)-l,3-(4-cWorober_zoxazol)-2-amine
Cis -N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)-l,3-(5-chlorobenzoxazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(6-chlorobenzoxazol)-2-amine
Cis -N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyπolo [2,3 - d]pyrimidin-5-yl}phenyl)-l,3-(7-chlorobenzoxazol)-2-amine

Cϊs-N2-(4- {4-amino-7-[4- 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- ,3-(4,5-dichlorobenzoxazol)-2-amine

Cϊs-N2-(4- {4-amino-7-[4- 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) ,3-(4,6-dichlorobenzoxazol)-2-amine

Os-N2-(4- {4-amino-7-[4. 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl) 3-(4,7-dichlorobenzoxazol)-2-amine

Os-N2-(4- {4-amino-7-[4' 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) 3-(5,6-dichlorolbenzoxazol)-2-amine

Cfc-N2-(4- {4-amino-7-[4 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) 3-(5,7-dichlorobenzoxazol)-2-amine

Cfe-N2-(4- {4-amino-7-[4 4-methylpiperazino)cyclohexyl]-7H-pyπolo 2,3-d]pyrimidm- 5 -yl} phenyl) 3-(6,7-dichlorobenzoxazol)-2-amine

Os-N2-(4- {4-amino-7-[4 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) ,3-(5-chloro-4-methylbenzoxazol)-2-amine

Os-N2-(4- {4-amino-7-[4- 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- ,3-(5-fluoro-4-methylbenzoxazol)-2-amine

Os-N2-(4- {4-amino-7-[4 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) ,3-(6-chloro-4-methylbenzoxazol)-2-amine

Cis-N2-(4- {4-amino-7-[4- 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) ,3-(6-fluoro-4-methylbenzoxazol)-2-amine

Cw-N2-(4- {4-amino-7-[4. 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) ,3-(7-chloro-4-methylbenzoxazol)-2-amine

Cis-N2-(4- {4-amino-7-[4 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) ,3-(7-fluoro-4-methylbenzoxazol)-2-amine

Cis-N2-(4- {4-amino-7-[4. 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl) 3-(4-chloro-5-methylbenzoxazol)-2-amine

Os-N2-(4- {4-amino-7-[4 4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl) ,3-(4-fluoro-5-methylbenzoxazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3- d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-5-methylbenzoxazol)-2-amine

Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidm-5-yl}phenyl)-l,3-(6-fluoro-5-methylbenzoxazol)-2-amine

Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-5-methylbenzoxazol)-2-amine

Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-fluoro-5-methylbenzoxazol)-2-amine

Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4-chloro-6-methylbenzoxazol)-2-amine Os-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-6-methylbenzoxazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-6-methylbenzoxazol)-2-amine

C/-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluoro-6-methylbenzoxazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-6-methylbenzoxazol)-2-amine

Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-6-methylbenzoxazol)-2-amine

Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chloro-7-methylbenzoxazol)-2-amine

Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolθ' 2,3-d]pyrimidin-5-yl}phenyl)-l ,3-(4-fluoro-7-methylbenzoxazol)-2-amine

Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-7-methylbenzoxazol)-2-amine C/_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5-fluoro-7-methylbenzoxazol)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-7-methylbeι__zoxazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3- d]pyrirnidin-5-yl}phenyl)-l,3-(6-fluoro-7-methylbeι__z;oxazol)-2-anιine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-benzothiazol-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4-methylbenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriιmdin-5-yl}phenyl)-l,3-(5-memylbe]__zothiazol)-2-amine
Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-methylbenzothiazol)-2-amine
Cis-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-methylbenzothiazol)-2-amine

Cω-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} phenyl)- 1 ,3-(4,5-dimethylbenzothiazol)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4,6-dimethylbenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,7-dimethylbenzothiazol)-2-amine
Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,6-dimethylbenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,7-dimethylbenzothiazol)-2-amine
Cti'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-dimethylbenzotMazol)-2-amine
Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluorobenzothiazol)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluorober_zothiazol)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyιiιm-din-5-yl}phenyl)-l,3-(6-fluorobeι__zotMazol)-2-amine
Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidin-5-yl}phenyl)-l,3-(7-fluorobenzothiazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3 -(4,5 -difluorobenzothiazol)-2-amine
C/-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,6-difluorobenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4,7-difluorobenzothiazol)-2-amine
Cz_;-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)- 1 ,3-(5 , 6-difluorolbenzothiazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(5,7-difluorobenzothiazol)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-difluorobenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chlorobenzothiazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} phenyl)- 1 ,3-(5-chlorobenzothiazol)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chlorobenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(7-chlorobenzothiazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l ,3-(4,5-dichlorobenzothiazol)-2-amine
Cis- -N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo [2,3 -d]pyrimidin-5-yl}phenyl)-l,3-(4,6-dichlorobenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(4,7-dichlorobenzothiazol)-2-amine
Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5,6-dichlorolbenzothiazol)-2-amine
Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidin-5-yl}phenyl)-l,3-(5,7-dichlorobenzothiazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6,7-dichlorobenzothiazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-4-methylbenzothiazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluoro-4-methylbenzothiazol)-2-amine

Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-4-methylbenzothiazol)-2-amine

Cz>s-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-fluoro-4-methylbenzothiazol)-2-amine

Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-4-methylbenzothiazol)-2-amine

Ct.s'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-4-methylbenzothiazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chloro-5-methylbenzothiazol)-2-amine

Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} phenyl)- 1 ,3-(4-fluoro-5-methylbenzothiazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-5-methylbenzothiazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-fluoro-5-methylbenzothiazol)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-5-methylbenzothiazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-fluoro-5-methylbenzothiazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chloro-6-methylbenzothiazol)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-6-methylbenzotMazol)-2-amine

Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-6-methylbenzothiazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrirnidin-5-yl}phenyl)-l,3-(5-fluoro-6-methylbenzothiazol)-2-amine

Cis-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(7-chloro-6-methylbenzothiazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrirnidin-5-yl}phenyl)- 1 ,3-(7-fluoro-6-methylbenzothiazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-chloro-7-methylbenzothiazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(4-fluoro-7-methylbenzothiazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-chloro-7-methylbenzothiazol)-2-amine Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(5-fluoro-7-methylbenzothiazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-l,3-(6-chloro-7-methylbenzothiazol)-2-amine

Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)- 1 ,3-(6-fluoro-7-methylbenzothiazol)-2-amine

Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-benzimidazol-2-amine
Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-methylbenzimidazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-methylbenzimidazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-methylbenzimidazol)-2-amine
Cts-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3- d]pynmidin-5-yl}phenyl)-(7-methylbenzimidazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,5-(hmethylbenzi]mdazol)-2-an___ne

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyriιmdin-5-yl}phenyl)-(4,6-dimethylbeι__ziι dazol)-2-amine
Cis-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dimethylbenzimidazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dimethylbenzimidazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dimethylbenzimidazol)-2-amine
Cz'-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dimethylbenzimidazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-fluorobenzimidazol)-2-amine
Cz'-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluorobenzimidazol)-2-amine
C/_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-fluorobenzimidazol)-2-amine
C/-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-fluorobenzimidazol)-2-amine
Ct-r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-difluorobenzimidazol)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-difluorobenzimidazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-difluorobenzimidazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-difluorolbenzimidazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3- d]pyrinfidιn-5-yl}phenyl)-(5,7-difluorobenzi]m-dazol)-2-an__ine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-difluorobenzimidazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-chlorobenzimidazol)-2-amine
Cis -N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-chlorobenzimidazol)-2-amine
Cw-N2-(4-{4-_unino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chlorobenzimidazol)-2-amine
Cz'_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chlorobenzimidazol)-2-amine
Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dichlorobenzimidazol)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dichlorobenzimidazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dichlorobenzimidazol)-2-amine
Cz'_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dichlorolbenzimidazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dichlorobenzimidazol)-2-amine
Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dichlorobenzimidazol)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} phenyl)-(5 -chloro-4-methylbenzimidazol)-2-amine Ct_j-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-4-methylbenzimidazol)-2-amine

Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyriιnidin-5-yl}phenyl)-(6-chloro-4-methylberιzimidazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidm-5-yl}phenyl)-(6-fluoro-4-methylberιzinfrdazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-4-methylberιzimidazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-4-methylbenzimidazol)-2-amine Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-5-methylbenzimidazol)-2-amine

Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-5-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-5-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-5-methylbeι__zimidazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-5-methylbenzimidazol)-2-amine Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidm-5-yl}phenyl)-(7-fluoro-5-methylberιzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-6-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-6-methylbenzimidazol)-2-amine

C/-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl}phenyl)-(5 -chloro-6-methylbenzimidazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-6-methylbenzimidazol)-2-amine Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-6-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-6-me1frylbenzimidazol)-2-amine Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyriιrήdin-5-yl}phenyl)-(4-chloro-7-methylbenzimidazol)-2-amine

Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidm-5-yl}phenyl)-(4-fluoro-7-methylberιzimidazol)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-7-methylbenzimidazol)-2-amine Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-7-methylbenzimidazol)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-7-methylbenzimidazol)-2-amine

Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pvrimimn-5-yl}phenyl)-(6-fluoro-7-methylbenzimidazol)-2-amine

Cis-~N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)- 1 -methylbenzimidazol-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethylbenzimidazol)-2-amine
Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,5-dimethylbenzimidazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,6-dimethylbenzimidazol)-2-amine
C^-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethylbenzimidazol)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l ,4,5-trimethylbenzimidazol)-2-amine
Cz5-N2-(4-{4-amino-7-[4-(4-methylpiρerazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l,4,6-trimethylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidm-5-yl}phenyl)-(l,4,7-trimethylber_zimidazol)-2-amine
Ct_r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(l ,5,6-trimethylbenzimidazol)-2-amine Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} pheny l)-( 1 ,5 ,7-trimethylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l ,6,7-trimethylbenzimidazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriι din-5-yl}phenyl)-(4-fluoro-l-methylbenzimidazol)-2-amine Cis-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-l-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-l-methylbenzimidazol)-2-amine

Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-l-methylbemzimidazol)-2-aιrιine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,5-difluoro- 1 -methylbenzimidazol)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,6-difluoro-l-methylbenzimidazol)-2-amine

Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,7-difluoro-l-methylbenzimidazol)-2-amine

Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,6-difluoro-l-methylbenzimidazol)-2-amine

Cz'_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,7-difluoro-l-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6,7-difluoro- 1 -methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} pheny l)-(4-chloro- 1 -methylbenzimidazol)-2-amine

Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro- 1 -methylbenzimidazol)-2-amine

C/5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl} pheny l)-(6-chloro-l -methylbenzimidazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro- 1 -methylbenzinfrdazol)-2-amine

Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyriιmdin-5-yl}phenyl)-(4,5-dichloro-l-methylbenzimidazol)-2-amine

Cz'-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dichloro-l-methylbenzimidazol)-2-amine Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} pheny l)-(4,7-dichlorobenzimidazol)-2-amine
Cz'5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dichloro-l-methylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dichloro-l-methylbenzimidazol)-2-amine

Ct>s-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dichloro-l-methylbenzimidazol)-2-amine

Ct5,-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l,4-dimethylbenzimidazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethyl-5-fluorobenzimidazol)-2-amine

Cz'5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-l,4-dimethylbenzimidazol)-2-amine

Cz'5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethyl-6-fluorobenzimidazol)-2-amine

Ct-r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-l,4-dimethylbenzimidazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,4-dimethyl-7-fluorobenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro- 1 ,5-dimethylbenzimidazol)-2-amine

Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,5-dimethyl-4-fluorobenzimidazol)-2-amine Ct5-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo [2,3 -d]pyrimidm-5-yl}phenyl)-(6-cWoro-l,5-dimethylbenzimidazol)-2-anιine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l ,5-dimethyl-6-fluorobenzimidazol)-2-amine

Cz'5'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-l,5-dimethylbenzimidazol)-2-amine Cis-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,5-dimethyl-7-fluorobenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-l,6-dimethylbenzimidazol)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,6-dimethyl-4-fluorobenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l,6-dimethylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,6-dimethyl-5-fluorobenzimidazol)-2-amine Cz'-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-l,6-dimethylbenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,6-dimethyl-7-fluorobenzimidazol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-l,7-dimethylbenzimidazol)-2-amine Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethyl-4-fluorobenzimidazol)-2-amine

C/_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-l,7-dimethylbenzimidazol)-2-amine

Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethyl-5-fluorobenzimidazol)-2-amine

Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}ρhenyl)-(6-chloro-l,7-dimethylbenzimidazol)-2-amine Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(l,7-dimethyl-6-fluorober_zimidazol)-2-amine

Cis -N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pyπolo 2,3-d]pyrimidin-5-yl}phenyl)-quinazolino-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-methylquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-methylquinazolino)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-methylquinazolino)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5 -yl} pheny l)-(7-methylquinazolino)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dimethylquinazolino)-2-amine
Cis-N2-(4- {4-amino-7- [4-(4-methylpiperazino)cyclohexyl] -7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dimethylquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dimethylquinazolino)-2-amine

Cz'-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-dimethylquinazolino)-2-amine
Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dimethylquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dimethylquinazolino)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoroquinazolino)-2-amine
Cz_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoroquinazolino)-2-amine
Cz'5'-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoroquinazolino)-2-amine Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidm-5-yl}phenyl)-(7-fluoroquinazolino)-2-aniine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-difluoroquinazolino)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-difluoroquinazolino)-2-amine
Cz's-N2-(4- {4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-difluoroquinazolino)-2-amine
C/_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,6-difluorolquinazolino)-2-amine
Ct-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5,7-difluoroquin__zolino)-2-amine
Ct-f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6,7-difluoroquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4-chloroquinazolino)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5-chloroquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(6-chloroquinazolino)-2-amine
Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(7-chloroquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,5-dichloroquinazolino)-2-amine
Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,6-dichloroquinazolmo)-2-amine
Ct_f-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(4,7-dichloroquinazolino)-2-amine
Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cycIohexyl]-7H-pynolo 2,3-d]pyrimidin-5-yl}phenyl)-(5 ,6-dichlorolquinazolino)-2-amine Ct_.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5,7-dichloroquinazohno)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6,7-dichloroquinazolino)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-4-methylquinazolino)-2-amine

C__?-N2-(4- {4-amino-7-[4-(4-mexhylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrirnidin-5-yl}phenyl)-(5-fluoro-4-methylquinazolino)-2-amine

Ct-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-4-methylquinazolino)-2-amine

Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-4-methylquinazolino)-2-amine
Cώ-N2-(4-{4-aπnno-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-4-methylquinazolino)-2-amine

C/5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-4-methylquinazolinol)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-5-methylquinazolino)-2-amine

Ct_?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-5-methylquinazolino)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-5-methylquinazolino)-2-amine

Ct5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-5-methylquinazolino)-2-amine
Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-5-methylquinazolino)-2-amine

Cώ-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrirnidin-5-yl}phenyl)-(7-fluoro-5-methylquinazolino)-2-amine
Ct5-N2-(4-{4-aιnino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-6-methylquinazolino)-2-amine Cz'5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-6-methylquinazolino)-2-amine

Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-6-methylquinazolino)-2-amine
Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5 -yl} phenyl)-(5 -fluoro-6-methylquinazolino)-2-amine
C/-.-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-chloro-6-methylquinazolino)-2-amine
Cz5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(7-fluoro-6-methylquinazolino)-2-amine
Cz'-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-chloro-7-methylquinazolino)-2-amine

Cz'5-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(4-fluoro-7-methylquinazolino)-2-amine
Cz-?-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-chloro-7-methylquinazolino)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(5-fluoro-7-methylquinazolino)-2-amine
Cw-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-chloro-7-methylquinazolino)-2-amine
Cz'_r-N2-(4-{4-amino-7-[4-(4-methylpiperazino)cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}phenyl)-(6-fluoro-7-methylquinazolino)-2-amine
In addition to the foregoing compounds, the conesponding 2-fluorophynyl analogs ofthe above list of compounds are also prefened, e.g., ct5-N2-(4-{4-_tmino-7-[4-(4-methylpiperazino)-cyclohexyl]-7H-pynolo[2,3-d]pyrimidin-5-yl}-3-fluorophynyl)-(6-fluoro-7-methylquinazolino)-2-amine.