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1. WO2001062972 - METHODS FOR IDENTIFYING CANDIDATE POLYNUCLEOTIDE MOLECULES ENCODING A PROTEASE

Publication Number WO/2001/062972
Publication Date 30.08.2001
International Application No. PCT/US2001/005389
International Filing Date 20.02.2001
Chapter 2 Demand Filed 25.09.2001
IPC
C07K 14/15 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
08RNA viruses
15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
C07K 14/705 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
C12N 9/64 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
48acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50Proteinases
64derived from animal tissue, e.g. rennin
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
C07K 14/005
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
005from viruses
C07K 14/70575
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
70575NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C07K 2319/50
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
50containing protease site
C12N 15/1037
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
Applicants
  • BIOFOCUS DISCOVERY LIMITED [GB]/[GB] (AllExceptUS)
  • RUSSELL, Stephen, J. [GB]/[US] (UsOnly)
  • CHADWICK, Mark, P. [GB]/[GB] (UsOnly)
  • BUCHHOLZ, Christian [DE]/[DE] (UsOnly)
Inventors
  • RUSSELL, Stephen, J.
  • CHADWICK, Mark, P.
  • BUCHHOLZ, Christian
Agents
  • WILLIAMS, Kathleen, M.
Priority Data
60/184,98225.02.2000US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHODS FOR IDENTIFYING CANDIDATE POLYNUCLEOTIDE MOLECULES ENCODING A PROTEASE
(FR) PROCEDES D'IDENTIFICATION DE MOLECULES POLYNUCLEOTIDIQUES CANDIDATES CODANT UNE PROTEASE
Abstract
(EN) Methods are disclosed whereby protease activity is directly linked to delivery of a transferable label to a target cell which expresses a protease, via fusion of viral display packages comprising the transferable label with the target cell. The methods can be used,inter alia, to identify proteases, including previously undiscovered proteases or variants of known proteases which may have altered substrate specificity.
(FR) La présente invention concerne des procédés selon lesquels l'activité de la protéase est directement liée à l'administration d'un marqueur transférable à une cellule cible exprimant une protéase, via la fusion de capsides virales contenant le marqueur transférable, avec la cellule cible. On peut utiliser ces procédés, inter alia, pour identifier les protéases, notamment les protéases non encore identifiées ou des variantes de protéases déjà connues qui pourraient avoir une spécificité altérée vis à vis du substrat.
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