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1. WO2001061029 - IDENTIFICATION METHOD

Publication Number WO/2001/061029
Publication Date 23.08.2001
International Application No. PCT/GB2001/000658
International Filing Date 16.02.2001
Chapter 2 Demand Filed 17.07.2001
IPC
C12Q 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
G01N 27/447 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
CPC
C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 2304/80
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
2304Chemical means of detecting microorganisms
80Electrochemical detection via electrodes in contact with culture medium
G01N 27/447
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
Applicants
  • ZETATRONICS LIMITED [GB]/[GB] (AllExceptUS)
  • GRANT, Kathleen, Ann [GB]/[GB] (UsOnly)
  • HARBRON, Stuart [GB]/[GB] (UsOnly)
  • WILLIAMS, David, Ross [GB]/[GB] (UsOnly)
Inventors
  • GRANT, Kathleen, Ann
  • HARBRON, Stuart
  • WILLIAMS, David, Ross
Agents
  • COATES, Ian, Harold
Priority Data
0003554.317.02.2000GB
0003795.219.02.2000GB
0007771.930.03.2000GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) IDENTIFICATION METHOD
(FR) PROCEDE D'IDENTIFICATION
Abstract
(EN)
A method of identifying one or more micro-organisms in a fluid sample, said method comprising the steps of: (i) optionally culturing the sample if necessary to increase the number of micro-organisms to a pre-determined range; (ii) applying an electric field across a portion of said fluid; (iii) measuring the velocity, displacement, zeta potential or electrophoretic mobility of any micro-organisms present following the application of said electric field; (iv) re-measuring the velocity, displacement, zeta potential or electrophoretic mobility of the fluid sample after incubation in the presence of a bioactive peptide; (v) comparing said measured velocities, displacements, zeta potentials or electrophoretic mobilities with tables of velocities, displacements, zeta potentials or electrophoretic mobilities of known micro-organisms measured under substantially identical experimental conditions to determine which, if any, micro-organisms are present.
(FR)
La présente invention concerne un procédé d'identification d'au moins un micro-organisme dans un échantillon fluide. Ce procédé comporte plusieurs opérations. (i) Mise en culture éventuelle de l'échantillon pour augmenter si nécessaire ne nombre de micro-organismes jusqu'à un niveau convenu. (ii) Application d'un champ électrique sur une partie du fluide considéré. (iii) Mesure de la vitesse, du déplacement, du potentiel zêta ou de la mobilité électrophorétique de tous les micro-organismes présents après application du champ électrique. (iv) Nouvelle mesure de la vitesse, du déplacement, du potentiel zêta ou de la mobilité électrophorétique de l'échantillon fluide après incubation en présence d'un peptide bioactif. (v) Comparaison des vitesses, déplacements, potentiels zêta ou mobilités électrophorétiques mesurés avec des tables de vitesses, déplacements, potentiels zêta ou mobilités électrophorétiques de micro-organismes connus dans des conditions expérimentales sensiblement identiques de façon à déterminer ceux des micro-organismes qui sont encore éventuellement présents.
Also published as
Latest bibliographic data on file with the International Bureau