Processing

Please wait...

Settings

Settings

Goto Application

1. WO2001057217 - EXPRESSION AND SECRETION VECTOR FOR HUMAN INTERFERON ALPHA AND PROCESS FOR PRODUCING HUMAN INTERFERON ALPHA BY EMPLOYING SAME

Publication Number WO/2001/057217
Publication Date 09.08.2001
International Application No. PCT/KR2001/000097
International Filing Date 19.01.2001
Chapter 2 Demand Filed 18.08.2001
IPC
C07K 14/245 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
24from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
245Escherichia (G)
C07K 14/56 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
52Cytokines; Lymphokines; Interferons
555Interferons (IFN)
56IFN-alpha
C12N 15/70 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
CPC
C07K 14/245
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
24from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
245Escherichia (G)
C07K 14/56
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
52Cytokines; Lymphokines; Interferons
555Interferons [IFN]
56IFN-alpha
C07K 2319/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
02containing a signal sequence
C12N 15/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
70Vectors or expression systems specially adapted for E. coli
Applicants
  • HANMI PHARM. CO. LTD. [KR]/[KR] (AllExceptUS)
  • KWON, Se, Chang [KR]/[KR] (UsOnly)
  • JUNG, Sung, Youb [KR]/[KR] (UsOnly)
  • CHOI, Ki, Doo [KR]/[KR] (UsOnly)
  • KIM, Cha, Soon [KR]/[KR] (UsOnly)
  • BAE, Sung, Min [KR]/[KR] (UsOnly)
  • LEE, Gwan, Sun [KR]/[KR] (UsOnly)
Inventors
  • KWON, Se, Chang
  • JUNG, Sung, Youb
  • CHOI, Ki, Doo
  • KIM, Cha, Soon
  • BAE, Sung, Min
  • LEE, Gwan, Sun
Agents
  • JANG, Seong, Ku
Priority Data
2000-243419.01.2000KR
Publication Language English (EN)
Filing Language Korean (KO)
Designated States
Title
(EN) EXPRESSION AND SECRETION VECTOR FOR HUMAN INTERFERON ALPHA AND PROCESS FOR PRODUCING HUMAN INTERFERON ALPHA BY EMPLOYING SAME
(FR) VECTEUR D"EXPRESSION ET DE SECRETION POUR L"INTERFERON HUMAIN ALPHA ET PROCEDE D"OBTENTION DE L"INTERFERON HUMAIN ALPHA AU MOYEN DUDIT VECTEUR
Abstract
(EN)
Disclosed in this invention are: an expression vector for the secretive production of human interferon alpha (hIFN$g(a)) comprising a polynucleotide encoding a modified E. coli thermostable enterotoxin II signal sequence and a polynucleotide encoding hIFN$g(a)) ligated to the 3"-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, said process being capable of secreting a soluble form of active hIFN$g(a)), which does not contain an additional methionine residue at its N-terminal, into the periplasm of an E. coli cell.
(FR)
Cette invention concerne: un vecteur d"expression pour la production par sécrétion de l"interféron humain alpha (hIFN$g(a)) comprenant un polynucléotide codant pour une séquence signal d"une entérotoxine thermostable de E.coli modifié et un polynucléotide codant pour (hIFN$g(a)) ligaturé sur l"extrémité 3" dudit interféron ; un micro-organisme transformé au moyen du vecteur d"expression; et un procédé d"obtention par sécrétion d"interféron humain par culture du micro-organisme, ledit procédé permettant de sécréter une forme soluble du (hIFN$g(a)) actif, qui ne renferme pas de résidu supplémentaire de méthionine sur son terminal N, dans le périplasme d"une cellule de E. coli.
Latest bibliographic data on file with the International Bureau