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1. WO2001046471 - EXPRESSION OF PROTEINS FROM AMPLIFIED, IMMOBILIZED NUCLEIC ACIDS

Publication Number WO/2001/046471
Publication Date 28.06.2001
International Application No. PCT/US2000/034426
International Filing Date 20.12.2000
Chapter 2 Demand Filed 20.12.2000
IPC
C12N 15/67 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/89 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
89using microinjection
C12P 19/34 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
CPC
C12N 15/67
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 15/89
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
89using microinjection
C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applicants
  • THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by THE SECRETARY OF THE NAVY [US]/[US]
Inventors
  • CHRISEY, Linda, A.
  • ANDREADIS, Joanne, D.
Agents
  • KARASEK, John, J.
Priority Data
09/468,61821.12.1999US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) EXPRESSION OF PROTEINS FROM AMPLIFIED, IMMOBILIZED NUCLEIC ACIDS
(FR) EXPRESSION DE PROTEINES A PARTIR D'ACIDES NUCLEIQUES IMMOBILISES ET AMPLIFIES
Abstract
(EN) A protein is made by immobilizing a PCR primer onto a solid support, then using the immobilized PCR primer along with a soluble primer to amplify and immobilize a template DNA containing a protein coding sequence onto the solid support. The template DNA and/or the PCR primers also contain regulatory sequences for transcription and translation of the coding sequence. The immobilized DNA is then transcribed and translated to produce the protein. The immobilized DNA may be reused for multiple cycles of transcription and translation. By immobilizing a universal PCR primer onto the solid support, different template DNAs can be amplified and immobilized and a number of different proteins can be made at the same time. If particles are used as the solid support, the particles may be injected into an organism so that the steps of transcription and translation take place within the organism to produce a protein vaccine or therapeutic.
(FR) L'invention concerne une protéine fabriquée par immobilisation d'une amorce PCR sur un support solide, puis par utilisation de cette amorce PCR avec une amorce soluble afin d'amplifier et d'immobiliser une ADN matrice contenant une séquence codante pour une protéine sur ledit support solide. L'ADN matrice et/ou les amorces PCR contiennent également des séquences régulatrices destinées à la transcription et à la traduction de ladite séquence codante. L'ADN immobilisé est ensuite transcrit et traduit de manière à produire une protéine. Cet ADN immobilisé peut être réutilisé pour divers cycles de transcription et de traduction. L'immobilisation d'une amorce PCR universelle sur un support solide permet d'amplifier et d'immobiliser différentes ADN matrices et de fabriquer simultanément différentes protéines. Si des particules sont utilisées comme support solides, celles-ci peuvent être injectées dans un organisme, de sorte que les étapes de transcription et de traduction s'effectuent à l'intérieur même dudit organisme afin de fabriquer un vaccin protéique ou un médicament.
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