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1. (WO2001038572) METHOD OF AMPLIFYING NUCLEIC ACIDS

Pub. No.:    WO/2001/038572    International Application No.:    PCT/JP2000/008073
Publication Date: Fri Jun 01 01:59:59 CEST 2001 International Filing Date: Fri Nov 17 00:59:59 CET 2000
IPC: C12N 15/10
C12Q 1/68
Applicants: TAKARA BIO INC.

AOYAGI, Kazuhiko

SASAKI, Hiroki

TERADA, Masaaki

MINENO, Junichi

ASADA, Kiyozo

KATO, Ikunoshin

Inventors: AOYAGI, Kazuhiko

SASAKI, Hiroki

TERADA, Masaaki

MINENO, Junichi

ASADA, Kiyozo

KATO, Ikunoshin

Title: METHOD OF AMPLIFYING NUCLEIC ACIDS
Abstract:
A method of amplifying nucleic acids which involves the following steps: (I) the step of forming a single-stranded DNA complementary to an mRNA in a sample with a first oligonucleotide primer; (II) the step of synthesizing a DNA strand complementary to the single-stranded DNA obtained in the step (I) to thereby form a double-stranded DNA; (III) the step of adding a single- or double-stranded adapter DNA to the double-stranded DNA obtained in the step (II) to give a DNA strand; and (IV) the step of amplifying the DNA strand obtained in the step (III) with the use of a second oligonucleotide primer which is an oligonucleotide having a nucleic acid sequence contained in the adapter DNA, and by which nucleic acids having base sequences of mRNAs contained in a sample can be amplified while sustaining the ratio among these mRNAs.