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1. (WO2001037958) ELECTRODELESS DIELECTROPHORESIS FOR POLARIZABLE PARTICLES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2001/037958 International Application No.: PCT/US2000/041929
Publication Date: 31.05.2001 International Filing Date: 06.11.2000
Chapter 2 Demand Filed: 31.05.2001
IPC:
B01L 3/00 (2006.01) ,B01L 7/00 (2006.01) ,B03C 5/02 (2006.01) ,C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01) ,G01N 1/40 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3
Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7
Heating or cooling apparatus; Heat insulating devices
B PERFORMING OPERATIONS; TRANSPORTING
03
SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
C
MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
5
Separating dispersed particles from liquids by electrostatic effect
02
Separators
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1
Sampling; Preparing specimens for investigation
28
Preparing specimens for investigation
40
Concentrating samples
Applicants:
PRINCETON UNIVERSITY [US/US]; Office of Technology Licensing and Intellectual Pr operty New South Building, 4th Floor P.O. Box 36 Princeton, NJ 08544-0036, US
Inventors:
AUSTIN, Robert, H.; US
TEGENFELDT, Jonas, O.; US
COX, Edward, C.; US
CHOU, Chia-Fu; US
BAKAJIN, Olgica; US
Agent:
MCKAY, Diane, Dunn; Mathews, Collins, Shepherd & Gould, P.A. Suite 306 100 Thanet Circle Princeton, NJ 08540, US
Priority Data:
60/163,52304.11.1999US
Title (EN) ELECTRODELESS DIELECTROPHORESIS FOR POLARIZABLE PARTICLES
(FR) DIELECTROPHORESE SANS ELECTRODE, POUR PARTICULES POLARISABLES
Abstract:
(EN) The present invention further provides a device for the micromanipulation, amplification, and analysis of polarized particles such as DNA. The device comprises a microchip (10) which contains constrictions (12) of insulating material for dielectrophoresis powered by an alternating current or direct current signal generator, and attached to a hot source that can be heated to specific temperatures. Nucleic acids can be heated and cooled to allow for denaturation, and the annealing of complementary primers and enzymatic reactions, as in a thermocycling reaction. After such a reaction has been completed at the constriction, the dielectrophoretic field can be switched to a direct field to release the product and direct it through a matrix for fractionation.
(FR) L'invention concerne un dispositif pour la micromanipulation, l'amplification et l'analyse intégrées de particules polarisées, telles que de l'ADN, qui comporte une micropuce contenant des étranglements de matériau isolant pour la diélectrophorèse alimentée par un générateur de signaux de courant alternatif ou continu, et fixée à une source chaude pouvant être portée à des températures spécifiques. Les acides nucléiques peuvent être chauffés et refroidis pour permettre la dénaturation et le recuit d'amorces complémentaires et des réactions enzymatiques, comme dans une réaction de thermocyclage. Une fois ladite réaction terminée au niveau de l'étranglement, le champ diélectrophorétique peut être commuté en champ direct, de sorte que le produit soit libéré et qu'il soit dirigé à travers une matrice afin qu'il soit fractionné. Ledit dispositif comprend un équipement d'analyse de données pour la commande de ces opérations, et un équipement d'imagerie pour l'analyse des produits. Le dispositif de l'invention permet la manipulation efficace d'échantillons minuscules en grand nombre, étant donné que les réactions ont lieu lorsque les échantillons sont piégés entre les étranglements. Etant donné que le positionnement, les réactions et la libération dans une matrice de fractionnement ont lieu au niveau de l'étranglement qui sert de site de concentration, la nécessité de transfert des échantillons dans différents tubes est supprimée, ce qui augmente l'efficacité et réduit le risque d'endommagement des échantillons.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AU2001045040