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1. (WO2001036681) IMMUNOLOGICAL DETECTION OF RNA:DNA HYBRIDS ON MICROARRAYS

Pub. No.:    WO/2001/036681    International Application No.:    PCT/US2000/031277
Publication Date: Sat May 26 01:59:59 CEST 2001 International Filing Date: Wed Nov 15 00:59:59 CET 2000
IPC: C12N 15/09
C12Q 1/68
G01N 31/22
G01N 33/53
G01N 33/543
G01N 33/577
G01N 37/00
Applicants: DIGENE CORPORATION
Inventors: LAZAR, James, G.
ZAKEL, Joan, M.
STRANGE, Christina, M.
WILLIAMS, Inna, R.
LORINCZ, Attila, T.
Title: IMMUNOLOGICAL DETECTION OF RNA:DNA HYBRIDS ON MICROARRAYS
Abstract:
The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.