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1. (WO2001036664) A METHOD FOR DETECTING CONTAMINATING MICROORGANISMS

Pub. No.:    WO/2001/036664    International Application No.:    PCT/SE2000/002218
Publication Date: Sat May 26 01:59:59 CEST 2001 International Filing Date: Tue Nov 14 00:59:59 CET 2000
IPC: C12M 1/34
G01N 33/00
G01N 33/497
Applicants: APPLIEDSENSOR SWEDEN AB
MANDENIUS, Carl-Fredik, Anton
BACHINGER, Thomas
Inventors: MANDENIUS, Carl-Fredik, Anton
BACHINGER, Thomas
Title: A METHOD FOR DETECTING CONTAMINATING MICROORGANISMS
Abstract:
A method for early detection of undesired microbial infection in a cell culture by means of an electronic nose having an array of gas sensors and the ability of pattern recognition is described, said method comprising the steps of withdrawing an off-gas sample containing volatile compounds from the cell culture in a bioreactor, passing said sample to the gas sensors of the electronic nose, and comparing any specific gas sensor signal patterns with standard signal patterns corresponding to different micro-organisms, wherein the off-gas sample is withdrawn from an animal cell culture, and the detection of any microbial infection is established about 1-50 h earlier for each micro-organism compared to known techniques.