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1. (WO2001036482) TRANSGENIC MICE EXPRESSING FLUORESCENT PROTEIN UNDER THE CONTROL OF THE NESTIN PROMOTER

Pub. No.:    WO/2001/036482    International Application No.:    PCT/US2000/031150
Publication Date: Sat May 26 01:59:59 CEST 2001 International Filing Date: Wed Nov 15 00:59:59 CET 2000
IPC: C07K 14/47
C12N 15/85
Applicants: COLD SPRING HARBOR LABORATORY
ENIKOLOPOV, Grigori, N.
MIGNONE, John
Inventors: ENIKOLOPOV, Grigori, N.
MIGNONE, John
Title: TRANSGENIC MICE EXPRESSING FLUORESCENT PROTEIN UNDER THE CONTROL OF THE NESTIN PROMOTER
Abstract:
Non-human transgenic mammals are produced which have, incorporated in their genome, DNA which includes a regulatory sequence of a mammalian nestin gene, operably linked to a gene coding for a marker/reporter protein. The regulatory sequence can include a promoter and a sequence present in the second intron of the mammmalian nestin gene. Preferably, the marker/reporter protein is a fluorescent protein, for example a green fluorescent protein, modified for enhanced fluorescence. Multipotent and, in particular, neural stem and progenitor cell populations are observed in the organs of the non-transgenic mammal or progeny thereof. Multipotent stem and progenitor cells are isolated directly from the non-human transgenic mammal, progeny or embryo thereof, for example by FACS, without culture passages.