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1. (WO2001032844) MICROTITER PLATE (MTP) BASED HIGH THROUGHPUT SCREENING (HTS) ASSAYS
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2001/032844 International Application No.: PCT/DK2000/000568
Publication Date: 10.05.2001 International Filing Date: 10.10.2000
Chapter 2 Demand Filed: 23.03.2001
IPC:
C07K 14/32 (2006.01) ,C07K 14/37 (2006.01) ,C12N 9/28 (2006.01) ,C12N 9/42 (2006.01) ,C12N 9/54 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
32
from Bacillus (G)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
37
from fungi
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
26
acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
28
Alpha-amylase from microbial source, e.g. bacterial amylase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
42
acting on beta-1, 4-glucosidic bonds, e.g. cellulase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
48
acting on peptide bonds, e.g. thromboplastin, leucine aminopeptidase (3.4)
50
Proteinases
52
derived from bacteria
54
bacteria being Bacillus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
NOVOZYMES A/S [DK/DK]; Krogshoejvej 36 DK-2880 Bagsvaerd, DK
Inventors:
PEDERSEN, Henrik; DK
LAMSA, Michael; US
HANSEN, Peter, Kamp; DK
FRISNER, Henrik; DK
VIND, Jesper; DK
ERNST, Steffen; DK
KONGSBAK, Lars; DK
JOERGENSEN, Birthe, Ravn; DK
BECK, Thomas, Christian; DK
HUSUM, Tommy, Lykke; DK
OSSOWSKI, Ingemar, Von; FI
Priority Data:
PA 1999 0160505.11.1999DK
Title (EN) MICROTITER PLATE (MTP) BASED HIGH THROUGHPUT SCREENING (HTS) ASSAYS
(FR) CRIBLAGES A HAUT RENDEMENT BASES SUR DES PLAQUES DE MICROTITRATION
Abstract:
(EN) The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. In a first aspect the invention relates to a method for high throughput screening (HTS) of a large population of host cells for production of a molecule of interest, the method comprising the steps of: a) arranging the host cells in a spatial array so each position in the spatial array is occupied by one cell, b) cultivating the host cells under growth conditions suitable for HTS, c) assaying each array position for production of the molecule of interest, and d) selecting the cells from those positions where the molecule was produced, as determined in step c).
(FR) La présente invention concerne une méthode permettant de réaliser des analyses destinées au criblage efficace et précis de grands nombres de populations cellulaires produisant des variants d'une molécule considérée. Selon un premier aspect, l'invention concerne une méthode de criblage à haut rendement réalisée sur une population importante de cellules hôtes et visant à détecter la production d'une molécule considérée, laquelle méthode consiste: a) à disposer les cellules hôtes selon un certain agencement spatial, de manière que chaque position dudit agencement spatial soit occupée par une seule cellule; b) à cultiver les cellules hôtes dans des conditions de croissance se prêtant à un criblage à haut rendement; c) à analyser chaque position de l'agencement pour y détecter la production de la molécule considérée; et d) à sélectionner les cellules provenant des positions où ladite molécule a été produite, tel que déterminé en c).
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)