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1. (WO2001031989) HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY

Pub. No.:    WO/2001/031989    International Application No.:    PCT/DK2000/000682
Publication Date: Fri May 11 01:59:59 CEST 2001 International Filing Date: Sat Dec 09 00:59:59 CET 2000
IPC: G01N 33/68
Applicants: NOVOZYMES A/S
Inventors: ROGGEN, Erwin, Ludo
ERNST, Steffen
PEDERSEN, Henrik
Title: HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY
Abstract:
The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. The present invention relates more specifically to a method for screening a library of protein variants for functional variants with reduced antibody binding capacity, comprising the steps of: (i) generating a diversified library of protein variants starting from a relevant protein backbone, (ii) transforming the library into suitable host cells, (iii) culturing host cells, (iv) sampling each cell culture, (v) analysing a sample by determining the antibody binding capacity of the variant protein, (vi) analysing a sample by determining the functionality of the variant protein.