Search International and National Patent Collections

1. (WO2001031056) DETECTION METHOD BY PCR

Pub. No.:    WO/2001/031056    International Application No.:    PCT/BE2000/000131
Publication Date: Fri May 04 01:59:59 CEST 2001 International Filing Date: Sat Oct 28 01:59:59 CEST 2000
IPC: C12Q 1/68
Applicants: UNIVERSITE DE LIEGE
ZORZI, Willy
MELEN, Laurence
ZORZI, Danièle
EL MOUALIJ, Benaissa
HEINEN, Ernst
Inventors: ZORZI, Willy
MELEN, Laurence
ZORZI, Danièle
EL MOUALIJ, Benaissa
HEINEN, Ernst
Title: DETECTION METHOD BY PCR
Abstract:
The invention concerns a method using PCR, in particular immuno-PCR, for detecting microbiological entities and in particular elements or target molecules. The detection method comprises a step which consists in fixing an entity A, more particularly an antigen, directly or via a capture agent, for example an antibody, to one or several surface(s) inside the receptacle(s) of a real time PCR measuring apparatus and a step which consists in associating with said element A a coupling system with a nucleic acid, for example a DNA marker, a step which consists in amplifying the marker nucleic acid. The successive measurements are carried out during the phase amplifying the marker nucleic acid, in the presence of a capture agent and other optional intermediate fixing agents. The detection method is quantitative and can be used in particular for prion polypeptide assay.