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1. (WO2001030993) METHOD OF DETECTING TARGET NUCLEIC ACID

Pub. No.:    WO/2001/030993    International Application No.:    PCT/JP2000/007430
Publication Date: Fri May 04 01:59:59 CEST 2001 International Filing Date: Wed Oct 25 01:59:59 CEST 2000
IPC: C12Q 1/68
Applicants: WAKUNAGA PHARMACEUTICAL CO., LTD.

MITANI, Yasumasa

Inventors: MITANI, Yasumasa

Title: METHOD OF DETECTING TARGET NUCLEIC ACID
Abstract:
A method of specifically detecting a target nucleic acid in a biological material characterized by comprising: (a) the step of bringing the target nucleic acid or a chain complementary thereto into contact with a first labeled nucleic acid primer complementary to either, or a primer set containing this primer to thereby form a primer extension product; (b) the step of denaturing the first nucleic acid primer extension product as described above to give a denatured chain; (c) the step of bringing this denatured chain into contact with a second labeled nucleic acid primer complementary thereto to form a both-labeled primer extension product; and (d) the step of developing this both-labeled primer extension product on a chromatographic carrier having specifically binding reagents which bind specifically to the labels of the first labeled nucleic acid primer and the second labeled nucleic acid primer; and a kit for detecting a target nucleic acid with the use of this method. Use of the detection method/detection kit makes it possible to conveniently, quickly and highly sensitively detect the presence or absence of the target nucleic acid in a sample.