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1. (WO2001029187) PROCESS FOR PRODUCING MICROORGANISM-ORIGIN TRANSGLUTAMINASE
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2001/029187    International Application No.:    PCT/JP2000/007135
Publication Date: 26.04.2001 International Filing Date: 13.10.2000
Chapter 2 Demand Filed:    06.02.2001    
IPC:
C12N 9/10 (2006.01)
Applicants: AJINOMOTO CO., INC. [JP/JP]; 15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-0031 (JP) (For All Designated States Except US).
TAGUCHI, Seiichi [JP/JP]; (JP) (For US Only).
MOMOSE, Haruo [JP/JP]; (JP) (For US Only)
Inventors: TAGUCHI, Seiichi; (JP).
MOMOSE, Haruo; (JP)
Agent: NAKAMURA, Minoru; Room 646, Shin-Tokyo Building, 3-1, Marunouchi 3-Chome, Chiyoda-Ku, Tokyo 100-8355 (JP)
Priority Data:
11/295649 18.10.1999 JP
Title (EN) PROCESS FOR PRODUCING MICROORGANISM-ORIGIN TRANSGLUTAMINASE
(FR) PROCEDE DE PRODUCTION DE TRANSGLUTAMINASE DERIVEE D'UN MICRO-ORGANISME
Abstract: front page image
(EN)A process for the secretory production of transglutaminase by using a microorganism. A process for the mass production of transglutaminase characterized by comprising culturing a bacterium belonging to the genus Streptomyces which has an expression plasmid carrying a transglutaminase gene originating in actinomycetes and the inherent (intact) promoter thereof, thus making the bacterium to secret protransglutaminase in the early stage to the medium stage of the culture, and then cutting off and removing the pro-structure with Streptomyces-origin protease, etc. in the latter stage of the culture to thereby give matured (active) transglutaminase.
(FR)La présente invention concerne un procédé de production sécrétoire de transglutaminase mettant en oeuvre un micro-organisme. Ce procédé de production en série de transglutaminase est caractérisé en ce qu'il comporte: la culture d'une bactérie appartenant au genre Streptomyces qui présente une expression plasmidique portant le gène de la transglutaminase dérivé des actinomycètes et le promoteur inné (intact) de ceux-ci, permettant ainsi à la bactérie de sécréter de la protransglutaminase à un stade précoce au milieu de culture, et ensuite la coupe et l'extraction de la pro-structure avec la protéase dérivée des Streptomyces, et ainsi de suite, dans le stade avancé de la culture afin d'obtenir de la transglutaminase mûrie (active).
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)