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1. WO2001020035 - METHODS AND COMPOSITIONS FOR LINEAR ISOTHERMAL AMPLIFICATION OF POLYNUCLEOTIDE SEQUENCES

Publication Number WO/2001/020035
Publication Date 22.03.2001
International Application No. PCT/US2000/025104
International Filing Date 13.09.2000
Chapter 2 Demand Filed 09.04.2001
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C12Q 1/68 (2006.01)
CPC
C12Q 1/6844
C12Q 1/6865
Applicants
  • NUGEN TECHNOLOGIES, INC. [US/US]; 821 Industrial Road San Carlos, CA 94070, US (AllExceptUS)
  • KURN, Nurith [IL/US]; US (UsOnly)
Inventors
  • KURN, Nurith; US
Agents
  • POLIZZI, Catherine, M. ; Morrison & Foerster LLP 755 Page Mill Road Palo Alto, CA 94304-1018, US
  • GOLDIN, Douglas, Michael; J.A. Kemp & Co. 14 South Square Gray's Inn London WC1R 5LX, GB
Priority Data
60/153,60413.09.1999US
60/175,78012.01.2000US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND COMPOSITIONS FOR LINEAR ISOTHERMAL AMPLIFICATION OF POLYNUCLEOTIDE SEQUENCES
(FR) PROCEDES ET COMPOSITIONS UTILES POUR L'AMPLIFICATION ISOTHERMIQUE LINEAIRE DE SEQUENCES DE POLYNUCLEOTIDES
Abstract
(EN)
The present invention provides novel isothermal, single primer linear nucleic acid amplification methods. Methods for amplifying complementary DNA using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. The invention further provides compositions and kits for practising said methods, as well as methods which use the amplification products.
(FR)
La présente invention concerne de nouveaux procédés d'amplification d'acide nucléique linéaire isothermique à amorce unique. On décrit des procédés permettant d'amplifier de l'ADN complémentaire à l'aide d'une amorce composite, d'une extension d'amorce, d'un déplacement de brin et facultativement d'une séquence de terminaison ; des procédés d'amplification d'ARN codant au moyen d'une amorce composite, d'une extension d'amorce, d'un déplacement de brin, facultativement d'une inversion de matrice, d'un oligonucléotide à fonction de propromoteur et de la transcription. On décrit également des compositions et des kits permettant de mettre en oeuvre lesdits procédés ainsi que des procédés dans lesquels on utilise les produits d'amplification.
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