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1. (WO2001006009) RESTRICTION SITE PROFILING
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2001/006009 International Application No.: PCT/US2000/018608
Publication Date: 25.01.2001 International Filing Date: 07.07.2000
Chapter 2 Demand Filed: 14.02.2001
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
ANANIEV, Evgueni [RU/US]; US (UsOnly)
PIONEER HI-BRED INTERNATIONAL, INC. [US/US]; 800 Capital Square 400 Locust Street Des Moines, IA 50309, US (AllExceptUS)
Inventors:
ANANIEV, Evgueni; US
Agent:
RAN, David, B. ; Darwin Building 7100 N.W. 62nd Avenue Johnston, IA 50131-1000, US
Priority Data:
60/144,67520.07.1999US
Title (EN) RESTRICTION SITE PROFILING
(FR) PROFILAGE DE SITES DE RESTRICTION
Abstract:
(EN) The invention provides a method of characterizing a polynucleotide by restriction site profiling. In this method, a partial digest of a sample of polynucleotide molecule is obtained using a restriction enzyme. A second partial digest is obtained from another sample of the same polynucleotide molecule using a different restriction enzyme which is not an isoschizomer of the restriction enzyme used to obtain the first partial digest. This process can be repeated any number of times with different samples and different non-isoschizomeric restriction enzymes. The linear order of restriction sites along the polynucleotide molecule is determined from the partial digests and provides the restriction site profile.
(FR) L"invention concerne une méthode de caractérisation d"un polynucléotide par profilage de sites de restriction. Dans cette méthode, un condensé partiel d"un échantillon de molécule polynucléotidique est obtenu à l"aide d"une enzyme de restriction. Un second condensé partiel est obtenu à partir d"un autre échantillon de la même molécule polynucléotidique à l"aide d"une enzyme de restriction différente laquelle n"est pas un isoschizomère de l"enzyme de restriction utilisée pour obtenir le premier condensé partiel. Ce processus peut être répété autant de fois avec différents échantillons et différentes enzymes de restriction isoschizomères. L"ordre linéaire de sites de restriction le long de la molécule polynucléotidique est déterminé à partir des condensés partiels et donne le profil des sites de restriction.
Designated States: AE, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, CA, CH, CN, CR, CU, CZ, DE, DK, DM, EE, ES, FI, GB, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)