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1. (WO2001005956) SIALYATION OF N-LINKED GLYCOPROTEINS IN THE BACULOVIRUS EXPRESSION VECTOR SYSTEM
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2001/005956    International Application No.:    PCT/US2000/019109
Publication Date: 25.01.2001 International Filing Date: 13.07.2000
Chapter 2 Demand Filed:    08.02.2001    
IPC:
C12N 5/07 (2010.01), C12N 9/16 (2006.01), C12P 21/00 (2006.01)
Applicants: BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC. [US/US]; Tower Road, Ithaca, NY 14853 (US)
Inventors: WOOD, H., Alan; (US)
Agent: BROWN, Michael, F.; Brown, Pinnisi & Michaels, P.C., 400 M & T Bank Building, 118 North Tioga Street, Ithaca, NY 14850 (US).
AQUILLA, Thomas, T.; Brown, Pinnisi & Michaels, P.C., 400 M & T Bank Building, 118 North Tioga Street, Ithaca, NY 14850 (US)
Priority Data:
09/353,897 15.07.1999 US
Title (EN) SIALYATION OF N-LINKED GLYCOPROTEINS IN THE BACULOVIRUS EXPRESSION VECTOR SYSTEM
(FR) SIALYATION DE GLYCOPROTEINES A LIAISON N DANS LE SYSTEME DE VECTEUR D'EXPRESSION DU BACULOVIRUS
Abstract: front page image
(EN)The present disclosure utilizes a novel approach to protein preparation in the baculovirus expression vector system (BEVS). Specifically, the present invention analyzes the effects of supplementing insect cell culture media with dexamethasone or N-acetylmannosamine on complex glycosylation of proteins prepared via BEVS, including the addition of terminal sialic acid residues to N-linked oligosaccharides.
(FR)La présente invention concerne une nouvelle approche pour la préparation de protéine dans le système de vecteur d'expression du baculovirus (SVEB). De manière plus spécifique, la présente invention analyse les effets de la supplémentation de milieux de culture de cellules d'insecte avec du dexaméthasone ou du N-acétylmannosamine sur la glycosylation complexe de protéines préparées par SVEB, y compris l'ajout de restes d'acide sialique terminal aux oligosaccharides à liaison N.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CR, CU, CZ, DE, DK, DM, DZ, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, UZ, VN, YU, ZA, ZW.
African Regional Intellectual Property Organization (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)