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1. (WO2001005936) TRANSFORMATION METHOD AND TRANSGENIC PLANTS PRODUCED THEREBY
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2001/005936 International Application No.: PCT/US2000/019721
Publication Date: 25.01.2001 International Filing Date: 18.07.2000
Chapter 2 Demand Filed: 16.02.2001
IPC:
C12N 15/82 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
CHRISTOU, Paul [US/GB]; GB (UsOnly)
KOHLI, Ajay [IN/GB]; GB (UsOnly)
PLANT BIOSCIENCE LTD. [GB/GB]; Norwich Research Park Colney Lane Norwich NR4 7UH, GB (AllExceptUS)
Inventors:
CHRISTOU, Paul; GB
KOHLI, Ajay; GB
Agent:
SCHOFIELD, Mary, Anne; Fulbright & Jaworski L.L.P. 801 Pennsylvania Avenue, NW Washington, DC 20004-2615, US
Priority Data:
09/611,73607.07.2000US
60/144,51319.07.1999US
Title (EN) TRANSFORMATION METHOD AND TRANSGENIC PLANTS PRODUCED THEREBY
(FR) METHODE DE TRANSFORMATION ET PLANTES TRANSGENIQUES AINSI PRODUITES
Abstract:
(EN) This invention relates to methods for producing, at a high frequency, transgenic plants that contain little if any vector sequences, have simple integration patterns, contain few copies of the transgene at each locus, express the transgene at all stages of development and do not exhibit transgene silencing. The method comprises introducing minimal transgene expression cassettes, which are substantially or totally devoid of vector sequences, by direct DNA transfer, preferably by particle or microprojectile bombardment. This invention also relates to transformed plant cells, the transgenic plants regenerated therefrom, and subparts of the transgenic plants produced by the methods of this invention. The invention also includes all progeny and subsequent progeny (i.e., all subsequent generations) derived from primary transformants through selfing or crossing.
(FR) Cette invention a trait à des méthodes permettant de produire, à une cadence élevée, des plantes transgéniques contenant, éventuellement, de petites séquences de vecteurs, ayant des configurations d'intégration simples, contenant quelques copies du transgène à chaque locus, exprimant le transgène à tous les stades du développement et ne faisant pas montre de suppression du transgène. La méthode consiste à introduire des cassettes d'expression transgénique minimale, qui sont quasiment ou totalement exemptes de séquences de vecteurs, par transfert direct d'ADN, de préférence par bombardement de particules ou de microprojectiles. Cette invention a également trait à des cellules végétales transformées, à des plantes transgéniques régénérées à partir de celles-ci, et à des sous-parties des plantes transgéniques produites grâce aux méthodes selon l'invention. Elle porte, en outre, sur toute la descendance et la descendance ultérieure (ces à dire toutes les générations à venir) dérivée des premiers transformants par conjugaison intractonale ou croisement.
Designated States: AL, AM, AT, AU, AZ, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IL, IS, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ, TM, TR, TT, UA, UG, US, UZ, VN
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)