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1. (WO2001004324) METHOD FOR PREPARING 1,3-PROPANEDIOL BY A RECOMBINANT MICRO-ORGANISM IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS

Pub. No.:    WO/2001/004324    International Application No.:    PCT/FR2000/001981
Publication Date: Fri Jan 19 00:59:59 CET 2001 International Filing Date: Sat Jul 08 01:59:59 CEST 2000
IPC: C12N 9/88
C12P 7/18
Applicants: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA)
INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
SARCABAL, Patricia
CROUX, Christian
SOUCAILLE, Philippe
Inventors: SARCABAL, Patricia
CROUX, Christian
SOUCAILLE, Philippe
Title: METHOD FOR PREPARING 1,3-PROPANEDIOL BY A RECOMBINANT MICRO-ORGANISM IN THE ABSENCE OF COENZYME B12 OR ONE OF ITS PRECURSORS
Abstract:
The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.