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1. (WO2001004323) 11-ARACHIDONATE-LIPOXYGENASE MUTANTS

Pub. No.:    WO/2001/004323    International Application No.:    PCT/EP2000/006539
Publication Date: Fri Jan 19 00:59:59 CET 2001 International Filing Date: Tue Jul 11 01:59:59 CEST 2000
IPC: C12N 9/02
C12N 15/82
C12P 7/64
Applicants: INSTITUT FÜR PFLANZENBIOCHEMIE - IPB
FEUSSNER, Ivo
HORNUNG, Ellen
ROSAHL, Sabine
Inventors: FEUSSNER, Ivo
HORNUNG, Ellen
ROSAHL, Sabine
Title: 11-ARACHIDONATE-LIPOXYGENASE MUTANTS
Abstract:
The invention relates to a method for producing a plant lipoxygenase with modified positional specificity toward arachidonic acid and to its utilization for hydroperoxydation of arachidonic acid. The inventive LOX makes it possible to produce for the first time (11S,14Z,12E,8Z,5Z)-11-hydroperoxy-14,12,8,5- eicosatetraenic acids at a large scale. To this end, arachidonic acid is incubated as substrate with the inventive LOX under appropriate conditions. Hydroperoxydation of the arachidonic acid is then effected, preferably in position 11, with secondary products which are hydroperoxylated in position 8 or position 5 or position 11 and 8 and 5.