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1. (WO2001004313) METHODS AND COMPOSITIONS FOR PREVENTING THE FORMATION OF ABERRANT RNA DURING TRANSCRIPTION OF A PLASMID SEQUENCE

Pub. No.:    WO/2001/004313    International Application No.:    PCT/US2000/017670
Publication Date: Fri Jan 19 00:59:59 CET 2001 International Filing Date: Wed Jun 28 01:59:59 CEST 2000
IPC: C07K 14/54
C12N 15/63
C12N 15/67
Applicants: AMERICAN HOME PRODUCTS CORPORATION
SATISHCHANDRAN, C.
PACHUK, Catherine, J.
Inventors: SATISHCHANDRAN, C.
PACHUK, Catherine, J.
Title: METHODS AND COMPOSITIONS FOR PREVENTING THE FORMATION OF ABERRANT RNA DURING TRANSCRIPTION OF A PLASMID SEQUENCE
Abstract:
Polynucleotide molecules, which include single stranded DNA or RNA, partially double-stranded DNA, and double-stranded DNA molecules, contain terminator sequences and/or other modifications which suppress the production of unwanted polynucleotide species from these molecules when transfected in a host cell. These molecules are useful in methods for enhancing the efficiency of transcription of a selected polynucleotide sequence in a transfected host cell, and reducing the potential for the products of unwanted transcripts. Further, the methods of the invention are useful in avoiding extinguishing or down regulating the expression of certain polynucleotides present in a host cell or host. These compositions and methods are useful in therapeutic, vaccine, diagnostic and research fields.