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1. (WO2001004306) EXPRESSION VECTORS AND METHODS

Pub. No.:    WO/2001/004306    International Application No.:    PCT/US2000/018841
Publication Date: Fri Jan 19 00:59:59 CET 2001 International Filing Date: Wed Jul 12 01:59:59 CEST 2000
IPC: C07K 14/435
C07K 14/71
C07K 16/42
C12N 15/10
C12N 15/65
C12N 15/69
C12N 15/85
Applicants: GENENTECH, INC.
CHISHOLM, Vanessa
CROWLEY, Craig, W.
KRUMMEN, Lynne, A.
MENG, Yu-Ju, G.
Inventors: CHISHOLM, Vanessa
CROWLEY, Craig, W.
KRUMMEN, Lynne, A.
MENG, Yu-Ju, G.
Title: EXPRESSION VECTORS AND METHODS
Abstract:
Vectors and methods for efficient isolation of recombinant cells expressing high levels of a desired protein are provided. The vectors comprise an amplifiable selectable gene, a fluorescent protein gene, and a gene encoding a desired product in a manner that optimizes transcriptional and translational linkage.