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1. (WO2001003647) MICROBIAL PROCESS FOR PREPARING PRAVASTATIN

Pub. No.:    WO/2001/003647    International Application No.:    PCT/US2000/019384
Publication Date: Fri Jan 19 00:59:59 CET 2001 International Filing Date: Wed Jul 12 01:59:59 CEST 2000
IPC: C12P 7/42
C12P 17/06
Applicants: IVAX CORPORATION
JEKKEL, Antonia
AMBRUS, Gabor
ILKOY, Eva
HORVATH, Ildiko
KONYA, Attila
SZABO, Istvan, Mihaly
NAGY, Zsuzsanna
HORVATH, Gyula
MOZES, Julianna
BARTA, Istvan
SOMOGYI, Gyorgy
SALAT, Janos
BOROS, Sandor
Inventors: JEKKEL, Antonia
AMBRUS, Gabor
ILKOY, Eva
HORVATH, Ildiko
KONYA, Attila
SZABO, Istvan, Mihaly
NAGY, Zsuzsanna
HORVATH, Gyula
MOZES, Julianna
BARTA, Istvan
SOMOGYI, Gyorgy
SALAT, Janos
BOROS, Sandor
Title: MICROBIAL PROCESS FOR PREPARING PRAVASTATIN
Abstract:
The present invention relates to a new microbial process for the preparation of the compound of formula (I) from a compound of general formula (II) wherein R+ stands for an alkali metal or ammonium ion, by the submerged cultivation of the strain able to 6β-hydroxylate a compound of formula (II) in aerobic fermentation and by the separation and purification of the product of formula (I) formed in the course of the bioconversion, which comprises cultivating a strain of the genera Micromonospora able to 6β-hydroxylate a compound of the general formula (II), wherein R+ is as defined above, on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts at 25-32 °C, thereafter feeding the substrate to be transformed into the developed culture, then fermenting the substrate until the end of bioconversion, then separating the compound of formula (I) from the culture broth and, if desired, purifying the same.